FastQCFastQC Report
Fri 17 Jun 2016
SRR1527859_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527859_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4566613
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATTGTGGGGAGTGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAAAAAAAAA868891.9027011923278807No Hit
TATTGTGGGAGTGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAAAAAAAAAA331770.7265121874789915No Hit
TATTGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA255480.5594518300543532No Hit
TATTGTGGGGTGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAAAAAAAAAAA223250.4888743583045027No Hit
TATTGTGGGCAGAGTGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAAAAAAA174920.3830409977810688No Hit
TATTGTGGGGAGTGCAGTGCTTATTGTGGGTAAAAAAAAAAAAAAAAAAAAA156440.34257336892791224No Hit
TATTGTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTATTGTGGGAAG151450.3316462332148575No Hit
TATTGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC140290.3072079898165227No Hit
TATTGTGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA115940.2538861953049229Illumina Single End Adapter 2 (100% over 34bp)
TATTGTGGGGAGTGCAGTGCTTATTGTGGGGAAAAAAAAAAAAAAAAAAAAA110370.24168897167331674No Hit
TATTGTGGGAGAGTGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAAAAAAAA90670.19854977857768985No Hit
TATTGTGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA88210.1931628539576268Illumina Single End Adapter 2 (97% over 34bp)
TATTGTGGGGAGTGCAGTGCTTATTGTGGGCAAAAAAAAAAAAAAAAAAAAA87300.1911701298095547No Hit
TATTGTGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA85360.1869219047026757Illumina Single End Adapter 2 (97% over 34bp)
TATTGTGGGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA78870.17271005885543617No Hit
TATTGTGGGGAGTGCAGTGCTTATTGGGGGAAAAAAAAAAAAAAAAAAAAAA76500.16752021684342422No Hit
TATTGTGGGTGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAAAAAAAAAAAA75160.16458587579021913No Hit
TATTGTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC73970.16198000575043253No Hit
TATTGTGGGACGCAGAGTGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAAAA71330.15619891591426732No Hit
TATTGTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG70240.15381202655009302No Hit
TATTGTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT70130.1535711478069195No Hit
TATTGTGGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA68060.14903824782174446Illumina Single End Adapter 2 (100% over 34bp)
TATTGTGGGAGTGCAGTGCTTATTGTGGGTAAAAAAAAAAAAAAAAAAAAAA56120.12289195515363356No Hit
TATTGTGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA56090.12282626095094985No Hit
TATTGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC55670.12190654211337812No Hit
TATTGTGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC55470.12146858076215349No Hit
TATTGTGGGAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATACCGTCGT52630.11524952957476362No Hit
TATTGTGGGGAGTGCAGTGCTTATTGGGGAAAAAAAAAAAAAAAAAAAAAAA52180.11426411653450819No Hit
TATTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA52140.11417652426426324No Hit
TATTGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT51730.11327870349425274No Hit
TATTGTGGGGCAGAGTGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAAAAAA49660.10874580350907774No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACGA206.313435E-446.041
CCGATAG850.045.99999621
GTGGGGG510800.040.5561875
TGGGGCT166150.040.3936166
GTGGGGC357400.040.3175745
TGGGATT73000.040.2657556
CGAATAG800.040.2524
CCGAATA800.040.2523
GTGGGGT501950.039.406325
TGTGGGG2343450.039.3525774
GTGGGAT225550.039.055645
TGGGGAG642950.038.36976
CGATAGG301.00203324E-438.33333244
TGGGGCC66000.038.2984856
TGGGGTG327550.038.212796
GTGGGAG554600.038.062395
TGGGGAC76700.037.9335066
TGGGGTC66400.037.8945776
CGAAAGT7300.037.80821615
TGGGGGC126000.037.8039676
Produced by FastQC (version 0.11.5)