##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527859_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4566613
Sequences flagged as poor quality	0
Sequence length	52
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.62888841248426	31.0	31.0	33.0	30.0	34.0
2	31.438536832440146	31.0	31.0	34.0	30.0	34.0
3	32.375867409828686	34.0	31.0	34.0	30.0	34.0
4	36.25039936600715	37.0	35.0	37.0	35.0	37.0
5	35.906594449759595	37.0	35.0	37.0	35.0	37.0
6	34.81863538688302	35.0	35.0	37.0	32.0	37.0
7	36.15268142932191	37.0	35.0	37.0	35.0	37.0
8	36.51153623046227	37.0	37.0	37.0	35.0	37.0
9	38.514236919134596	39.0	39.0	39.0	37.0	39.0
10	37.65406046012657	39.0	38.0	39.0	35.0	39.0
11	37.30156157309586	39.0	37.0	39.0	34.0	39.0
12	37.16441857455405	39.0	37.0	39.0	34.0	39.0
13	36.635036075971406	39.0	37.0	39.0	32.0	39.0
14	37.80559924828314	40.0	37.0	40.0	33.0	40.0
15	37.90901528112849	40.0	38.0	40.0	33.0	40.0
16	37.911733707235534	40.0	38.0	40.0	33.0	40.0
17	37.99168333292092	40.0	38.0	40.0	34.0	40.0
18	37.87363260254372	40.0	38.0	40.0	33.0	40.0
19	37.92758374751703	40.0	38.0	40.0	34.0	40.0
20	37.98916702597746	40.0	38.0	40.0	34.0	40.0
21	38.044378404738914	40.0	38.0	40.0	34.0	40.0
22	37.935190479245776	40.0	38.0	40.0	34.0	40.0
23	37.77533830872027	40.0	37.0	40.0	33.0	40.0
24	37.67856571161165	39.0	37.0	40.0	33.0	40.0
25	37.284920574614056	39.0	37.0	40.0	33.0	40.0
26	36.64819024515543	38.0	35.0	40.0	32.0	40.0
27	36.35554600313186	38.0	35.0	40.0	31.0	40.0
28	36.34580442879657	38.0	35.0	40.0	31.0	40.0
29	36.24137451542314	38.0	35.0	40.0	31.0	40.0
30	35.972487486896746	38.0	35.0	40.0	30.0	40.0
31	35.65326249454464	38.0	35.0	40.0	29.0	40.0
32	35.41656518737191	38.0	35.0	40.0	27.0	40.0
33	35.06824467061255	38.0	35.0	40.0	25.0	40.0
34	34.92628278332322	38.0	35.0	40.0	24.0	40.0
35	34.7367968338898	38.0	34.0	40.0	23.0	40.0
36	34.28545642032728	38.0	34.0	40.0	20.0	40.0
37	33.873470775824444	38.0	33.0	40.0	15.0	40.0
38	33.27264692672666	38.0	33.0	40.0	12.0	40.0
39	32.97018183936322	38.0	33.0	40.0	10.0	40.0
40	32.56143710885945	37.0	32.0	40.0	10.0	40.0
41	32.03294783245263	37.0	31.0	40.0	9.0	40.0
42	31.76461745280364	35.0	31.0	39.0	9.0	40.0
43	31.572977828425575	35.0	31.0	39.0	9.0	40.0
44	31.344552516274096	35.0	30.0	39.0	9.0	40.0
45	31.072259462319227	35.0	30.0	39.0	9.0	40.0
46	30.713032175049648	35.0	28.0	38.0	9.0	40.0
47	30.390267360076276	35.0	27.0	38.0	8.0	40.0
48	30.277311215117198	35.0	27.0	38.0	8.0	40.0
49	30.077732446344807	35.0	27.0	38.0	8.0	40.0
50	29.70314322671967	35.0	25.0	38.0	8.0	40.0
51	29.617169880609545	35.0	24.0	38.0	8.0	40.0
52	29.111566274610965	35.0	23.0	37.0	8.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	4.0
12	18.0
13	18.0
14	32.0
15	67.0
16	238.0
17	731.0
18	1845.0
19	4294.0
20	8419.0
21	15106.0
22	25670.0
23	41657.0
24	62034.0
25	82672.0
26	112202.0
27	141738.0
28	160620.0
29	147679.0
30	121401.0
31	120091.0
32	144455.0
33	186102.0
34	287324.0
35	357190.0
36	539111.0
37	904012.0
38	897586.0
39	204294.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	0.0	100.0	0.0	0.0
3	0.0	0.0	100.0	0.0
4	0.0	0.0	100.0	0.0
5	100.0	0.0	0.0	0.0
6	0.0	0.0	100.0	0.0
7	99.488242161094	0.2858354758767603	0.16710415355976083	0.05881820946946895
8	98.21705933916449	1.2525256683673436	0.30952918497801324	0.2208858074901464
9	94.79213587838514	2.813704599010251	1.1271154354441684	1.2670440871604403
10	46.30223756644148	34.26307418649227	9.29196321212242	10.142725034943842
11	26.366893800722767	43.78617150172349	16.813949419405585	13.03298527814816
12	36.782709636222734	29.801233430553452	18.614058165209094	14.801998768014718
13	21.796394833545126	30.617002141412026	26.371623783315997	21.214979241726855
14	29.030793719546633	33.35036273054012	18.909463096610114	18.709380453303137
15	24.607318377975098	31.84285596348979	19.470995243082783	24.078830415452327
16	25.064418640248253	38.42843700571956	21.147598887840946	15.359545466191246
17	32.66942042165605	31.08121927564258	19.298766941713694	16.95059336098767
18	25.327020266442545	32.55931693795818	24.950504892794726	17.163157902804553
19	29.624187554320898	30.13163147391732	21.67083131414902	18.573349657612763
20	20.426780197927876	31.310097877792582	24.37949088306804	23.883631041211505
21	22.298517522724172	31.833439794438462	30.81217961758529	15.055863065252082
22	20.651848536322216	31.227016609465263	30.223340580863763	17.89779427334876
23	21.915169951997246	37.638529912650796	25.76327356839741	14.683026566954545
24	24.23207309224583	30.104105602992853	31.096219451922025	14.567601852839292
25	25.09006127736246	29.221920053221062	28.10476823851726	17.583250430899223
26	32.38308129022538	28.18143337304913	24.123064511926014	15.312420824799474
27	28.08530085645532	28.03532946628059	26.384806419987854	17.494563257276237
28	35.48117609265335	31.658802705637633	17.02489350422293	15.83512769748608
29	34.65176488570413	31.256075345119015	18.818345237487826	15.273814531689023
30	31.961718674212158	34.26747569807207	17.553315772543023	16.21748985517275
31	28.09741048781668	38.77217097222822	16.99071062075985	16.139707919195253
32	26.207344480471633	40.694492833090955	18.131140081281245	14.967022605156163
33	26.030670871387613	41.06719794298313	17.769537291642624	15.132593893986638
34	23.456136966281136	42.71737062019488	18.15656373771984	15.669928675804146
35	22.771012126492874	44.57548734696809	18.093760079954222	14.55974044658481
36	24.044822716529733	42.970577975405405	17.387613095307177	15.596986212757683
37	24.62939163007682	42.731144504690896	17.322882407596175	15.316581457636108
38	25.35980167358171	41.27139742299161	17.001112202851434	16.36768870057524
39	24.832321022166756	39.72423325558789	17.284232318350604	18.159213403894746
40	24.011778532579836	39.20921260461528	18.994449496815253	17.78455936598963
41	23.761286537746905	38.50628463589973	19.921307104411955	17.811121721941404
42	23.629087903879746	38.78301051567102	19.001194101624115	18.586707478825115
43	23.35704821056656	37.85413828585869	20.653556585591993	18.13525691798276
44	23.710877186220948	38.09002426962827	20.081929429973595	18.11716911417718
45	24.507879253179546	38.194675134503406	19.26559136935843	18.031854242958623
46	23.839221760197326	38.896989957327236	19.257927045711998	18.00586123676344
47	24.452258161574015	38.34620976202713	18.943054732249042	18.258477344149814
48	24.60834758715048	37.96724180481245	19.112983736524203	18.31142687151287
49	24.455367687167712	37.49413405515203	19.34184044060664	18.708657817073618
50	22.2350131267966	38.348290078445444	19.681129099400366	19.735567695357588
51	21.823373252780563	38.29737707136559	20.20118630591206	19.678063369941793
52	22.535323225331332	36.97882872930113	20.7578789794537	19.72796906591384
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	148.0
2	296.0
3	382.0
4	468.0
5	1276.5
6	2085.0
7	19928.5
8	37772.0
9	28823.5
10	19875.0
11	17580.5
12	15286.0
13	15797.0
14	18885.0
15	21462.0
16	24478.0
17	27494.0
18	33067.5
19	38641.0
20	48384.0
21	58127.0
22	76075.5
23	94024.0
24	114657.5
25	135291.0
26	182069.0
27	228847.0
28	265145.5
29	301444.0
30	259829.5
31	218215.0
32	191600.0
33	164985.0
34	163179.0
35	161373.0
36	157902.0
37	154431.0
38	145263.0
39	137610.5
40	139126.0
41	151166.0
42	163206.0
43	159679.0
44	156152.0
45	163908.5
46	171665.0
47	202945.0
48	234225.0
49	260358.5
50	286492.0
51	279648.5
52	272805.0
53	243670.5
54	214536.0
55	216388.5
56	218241.0
57	205830.5
58	193420.0
59	188697.5
60	183975.0
61	163924.5
62	143874.0
63	133289.5
64	97311.0
65	71917.0
66	61562.5
67	51208.0
68	45797.5
69	40387.0
70	40347.5
71	40308.0
72	29176.5
73	18045.0
74	12413.5
75	6782.0
76	4972.5
77	3163.0
78	2160.5
79	1158.0
80	746.5
81	335.0
82	328.5
83	322.0
84	171.5
85	21.0
86	14.5
87	8.0
88	8.5
89	9.0
90	9.0
91	4.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	4566613.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.580684863166322
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.52821645964781	19.30277341661113
2	9.252200700117978	4.548508594007336
3	3.7441331529405355	2.761000713544938
4	1.785690307686981	1.7557396286585676
5	1.0504801839061553	1.2910761177799102
6	0.6719648944525478	0.9910414385789337
7	0.48253494519345763	0.8302727596285929
8	0.37755963347253957	0.7424539493952862
9	0.28514823607784373	0.6308225037285506
>10	2.5336530183658925	13.773817800657234
>50	0.7819215600019247	13.592563884452694
>100	0.4624967334008973	20.484839791089296
>500	0.027799273577877023	4.601493682626152
>1k	0.013439383915118605	5.876822842498423
>5k	0.001841011495221727	3.094942666867369
>10k+	9.205057476108635E-4	5.721830209875645
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATTGTGGGGAGTGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAAAAAAAAA	86889	1.9027011923278807	No Hit
TATTGTGGGAGTGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAAAAAAAAAA	33177	0.7265121874789915	No Hit
TATTGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	25548	0.5594518300543532	No Hit
TATTGTGGGGTGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAAAAAAAAAAA	22325	0.4888743583045027	No Hit
TATTGTGGGCAGAGTGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAAAAAAA	17492	0.3830409977810688	No Hit
TATTGTGGGGAGTGCAGTGCTTATTGTGGGTAAAAAAAAAAAAAAAAAAAAA	15644	0.34257336892791224	No Hit
TATTGTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTATTGTGGGAAG	15145	0.3316462332148575	No Hit
TATTGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	14029	0.3072079898165227	No Hit
TATTGTGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA	11594	0.2538861953049229	Illumina Single End Adapter 2 (100% over 34bp)
TATTGTGGGGAGTGCAGTGCTTATTGTGGGGAAAAAAAAAAAAAAAAAAAAA	11037	0.24168897167331674	No Hit
TATTGTGGGAGAGTGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAAAAAAAA	9067	0.19854977857768985	No Hit
TATTGTGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA	8821	0.1931628539576268	Illumina Single End Adapter 2 (97% over 34bp)
TATTGTGGGGAGTGCAGTGCTTATTGTGGGCAAAAAAAAAAAAAAAAAAAAA	8730	0.1911701298095547	No Hit
TATTGTGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA	8536	0.1869219047026757	Illumina Single End Adapter 2 (97% over 34bp)
TATTGTGGGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA	7887	0.17271005885543617	No Hit
TATTGTGGGGAGTGCAGTGCTTATTGGGGGAAAAAAAAAAAAAAAAAAAAAA	7650	0.16752021684342422	No Hit
TATTGTGGGTGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAAAAAAAAAAAA	7516	0.16458587579021913	No Hit
TATTGTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	7397	0.16198000575043253	No Hit
TATTGTGGGACGCAGAGTGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAAAA	7133	0.15619891591426732	No Hit
TATTGTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	7024	0.15381202655009302	No Hit
TATTGTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	7013	0.1535711478069195	No Hit
TATTGTGGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA	6806	0.14903824782174446	Illumina Single End Adapter 2 (100% over 34bp)
TATTGTGGGAGTGCAGTGCTTATTGTGGGTAAAAAAAAAAAAAAAAAAAAAA	5612	0.12289195515363356	No Hit
TATTGTGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5609	0.12282626095094985	No Hit
TATTGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC	5567	0.12190654211337812	No Hit
TATTGTGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC	5547	0.12146858076215349	No Hit
TATTGTGGGAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATACCGTCGT	5263	0.11524952957476362	No Hit
TATTGTGGGGAGTGCAGTGCTTATTGGGGAAAAAAAAAAAAAAAAAAAAAAA	5218	0.11426411653450819	No Hit
TATTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5214	0.11417652426426324	No Hit
TATTGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	5173	0.11327870349425274	No Hit
TATTGTGGGGCAGAGTGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAAAAAA	4966	0.10874580350907774	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.01377388449601488	0.0	0.0	0.0	0.0
8	0.15661497919793071	0.0	0.0	0.0	0.0
9	0.21766679155864532	0.0	0.0	0.0	0.0
10	0.6218175264687417	0.0	0.0	0.0	0.0
11	1.0355158188355351	0.0	0.0	0.0	0.0
12	1.1555391271386475	0.0	0.0	0.0	0.0
13	1.269080607443635	0.0	0.0	0.0	0.0
14	1.3722643017921599	0.0	0.0	0.0	0.0
15	1.5000395260119481	0.0	0.0	0.0	0.0
16	1.6669465969636577	0.0	0.0	0.0	0.0
17	1.7837946854703912	0.0	0.0	0.0	0.0
18	1.898584355626369	0.0	0.0	0.0	0.0
19	2.0123886127420914	0.0	0.0	0.0	0.0
20	2.1412806384075025	0.0	0.0	0.0	2.1898067561231924E-5
21	2.2686835954787496	0.0	0.0	0.0	2.1898067561231924E-5
22	2.4161451824360856	0.0	0.0	0.0	2.1898067561231924E-5
23	2.571862340864006	0.0	0.0	0.0	2.1898067561231924E-5
24	2.725236406062874	0.0	0.0	0.0	2.1898067561231924E-5
25	2.866873107048922	0.0	0.0	0.0	2.1898067561231924E-5
26	3.00886017711595	0.0	0.0	0.0	2.1898067561231924E-5
27	3.1372485472274527	0.0	0.0	0.0	2.1898067561231924E-5
28	3.2746808192417443	0.0	0.0	0.0	2.1898067561231924E-5
29	3.397463284057572	0.0	0.0	0.0	2.1898067561231924E-5
30	3.5376328145170173	2.1898067561231924E-5	0.0	0.0	6.569420268369577E-5
31	3.6840651922989753	2.1898067561231924E-5	0.0	0.0	8.75922702449277E-5
32	3.829468360905555	2.1898067561231924E-5	0.0	0.0	8.75922702449277E-5
33	3.9527106851401683	2.1898067561231924E-5	0.0	0.0	8.75922702449277E-5
34	4.072142745619128	2.1898067561231924E-5	0.0	0.0	8.75922702449277E-5
35	4.213582363997125	2.1898067561231924E-5	0.0	0.0	8.75922702449277E-5
36	4.3254376930998975	2.1898067561231924E-5	0.0	0.0	8.75922702449277E-5
37	4.446139841497407	2.1898067561231924E-5	0.0	0.0	1.0949033780615962E-4
38	4.5686595295025	2.1898067561231924E-5	0.0	0.0	1.0949033780615962E-4
39	4.702018760950402	2.1898067561231924E-5	0.0	0.0	1.0949033780615962E-4
40	4.8511664991099535	2.1898067561231924E-5	0.0	0.0	1.0949033780615962E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTACGA	20	6.313435E-4	46.0	41
CCGATAG	85	0.0	45.999996	21
GTGGGGG	51080	0.0	40.556187	5
TGGGGCT	16615	0.0	40.393616	6
GTGGGGC	35740	0.0	40.317574	5
TGGGATT	7300	0.0	40.265755	6
CGAATAG	80	0.0	40.25	24
CCGAATA	80	0.0	40.25	23
GTGGGGT	50195	0.0	39.40632	5
TGTGGGG	234345	0.0	39.352577	4
GTGGGAT	22555	0.0	39.05564	5
TGGGGAG	64295	0.0	38.3697	6
CGATAGG	30	1.00203324E-4	38.333332	44
TGGGGCC	6600	0.0	38.298485	6
TGGGGTG	32755	0.0	38.21279	6
GTGGGAG	55460	0.0	38.06239	5
TGGGGAC	7670	0.0	37.933506	6
TGGGGTC	6640	0.0	37.894577	6
CGAAAGT	730	0.0	37.808216	15
TGGGGGC	12600	0.0	37.803967	6
>>END_MODULE