FastQCFastQC Report
Fri 17 Jun 2016
SRR1527858_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527858_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences471399
Sequences flagged as poor quality0
Sequence length50
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAGGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC69401.4722135600627069No Hit
GCAGGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG39130.8300823718336271No Hit
GCAGGAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC26130.5543074974702958No Hit
GCAGGAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT23610.5008495987475577No Hit
GCAGGAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG16880.3580830676348486No Hit
GCAGGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTG12310.26113759257020064No Hit
GCAGGAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT12160.25795557478908526No Hit
GCAGGAGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCT11890.2522279427830776No Hit
GCAGGAGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCT11430.2424697549209905No Hit
GCAGGAGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCG10000.21213451874102407No Hit
GCAGGAGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGC9990.21192238422228304No Hit
GCAGGAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG9220.19558802627922417No Hit
GCAGGAGGGGATCGTGGTGTGCCCAGCTCTTCCAAGGACTGCTGCGCTTC8440.1790415338174243No Hit
GCAGGAGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACAGCT8310.176283785073791No Hit
GCAGGAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT8030.17034401854904233No Hit
GCAGGAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA7720.16376784846807058No Hit
GCAGGAGGGGGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCAT7470.15846448549954498No Hit
GCAGGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTGCTTGCCTAGC7180.15231258445605528No Hit
GCAGGAGGGAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATACCGTC7010.14870629763745785No Hit
GCAGGAGGGGAAAATCCTTTCCTTTATGCCCTGTCCCTTCCCCAGCACTC6800.14425147274389635No Hit
GCAGGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGT6780.14382720370641433No Hit
GCAGGAGGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCC6730.14276653111270918No Hit
GCAGGAGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAA6590.13979664785033485No Hit
GCAGGAGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCG6420.13619036103173743No Hit
GCAGGAGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAG6350.13470541940055028No Hit
GCAGGAGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTC5950.1262200386509093No Hit
GCAGGAGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC5820.123462289907276No Hit
GCAGGAGGGGGGGCTGGAGAGATGGCTCAGAGGTTAAGAGCACTGACTGC5620.11921959953245552No Hit
GCAGGAGGGGAGCCGGGCGTGGTGGCGCATGCCTTTAATCCCAGCACTCG5320.11285556397022481No Hit
GCAGGAGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGC5280.11200702589526071No Hit
GCAGGAGGGGGGTGCTGGAGAGATGGCTCAGCGGTTAAGAGCGTTGACTG5090.10797647003918125No Hit
GCAGGAGGGGGGCTGGAAAGTTGGCTCAGCGGTTAAGAACACTGACTGCT4990.10585512485177101No Hit
GCAGGAGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTC4910.10415804870184281No Hit
GCAGGAGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCG4870.10330951062687871No Hit
GCAGGAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCAT4850.10288524158939666No Hit
GCAGGAGGGGACTGTCCCCATAGTAGAGGCCACAGGAAGACCGTCGTGGA4830.10246097255191462No Hit
GCAGGAGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGT4760.10097603092072746No Hit
GCAGGAGGGGGTCTTCCGGTCGGGCCGAGCTCGTCGCTAGGTTTGTTGAA4760.10097603092072746No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAC600.044.00000411
GATAACC302.5245263E-644.0000049
CTCAAAT302.5245263E-644.00000424
TAACGAA302.5245263E-644.00000432
TACGACA302.5245263E-644.00000443
CGCTAAG302.5245263E-644.00000444
TCGTAAG302.5245263E-644.00000414
TTAGTCT302.5245263E-644.00000439
CGTAACT302.5245263E-644.00000442
ACGTAAC302.5245263E-644.00000441
ACGCGAT254.438832E-544.012
AACGTTG207.85175E-444.022
CTAGATG408.294592E-944.029
ACAACCG254.438832E-544.036
AACGTAC408.294592E-944.043
AGCGTAT254.438832E-544.020
AATCCGT351.4436591E-744.024
GCGACGT207.85175E-444.016
CGTATAT207.85175E-444.019
AATCACT254.438832E-544.040
Produced by FastQC (version 0.11.5)