##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527858_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	471399
Sequences flagged as poor quality	0
Sequence length	50
%GC	59
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.26839471445633	33.0	31.0	34.0	31.0	34.0
2	32.80098175855273	34.0	31.0	34.0	31.0	34.0
3	32.938506445707354	34.0	31.0	34.0	31.0	34.0
4	36.40872169860352	37.0	37.0	37.0	35.0	37.0
5	36.45754445809177	37.0	37.0	37.0	35.0	37.0
6	35.483238191001675	37.0	35.0	37.0	33.0	37.0
7	36.276390064467684	37.0	35.0	37.0	35.0	37.0
8	36.60519432582589	37.0	37.0	37.0	35.0	37.0
9	38.737890831333964	39.0	39.0	39.0	39.0	39.0
10	38.17784297378654	39.0	39.0	39.0	37.0	39.0
11	37.752846314905206	39.0	38.0	39.0	35.0	39.0
12	36.95783402170985	39.0	35.0	39.0	34.0	39.0
13	36.76824515962062	39.0	35.0	39.0	33.0	39.0
14	37.49653902532674	40.0	36.0	40.0	33.0	40.0
15	37.57810262643748	40.0	36.0	40.0	33.0	40.0
16	37.58101523337979	40.0	36.0	40.0	33.0	40.0
17	37.33980343615494	39.0	36.0	40.0	33.0	40.0
18	37.25737856889811	39.0	36.0	40.0	33.0	40.0
19	37.20459950063534	39.0	36.0	40.0	32.0	40.0
20	36.995564267213126	39.0	35.0	40.0	32.0	40.0
21	37.00653374317722	39.0	35.0	40.0	32.0	40.0
22	37.051527474602196	39.0	35.0	40.0	32.0	40.0
23	37.03357877297152	39.0	35.0	40.0	32.0	40.0
24	36.9584492118142	39.0	35.0	40.0	32.0	40.0
25	36.93632994554507	38.0	35.0	40.0	32.0	40.0
26	36.76674748991831	38.0	35.0	40.0	32.0	40.0
27	36.68692763455162	38.0	35.0	40.0	32.0	40.0
28	36.40417353452171	38.0	35.0	40.0	31.0	40.0
29	36.75551072446059	39.0	35.0	40.0	32.0	40.0
30	36.96501053247886	39.0	35.0	40.0	32.0	40.0
31	37.00307595052175	39.0	35.0	40.0	33.0	40.0
32	36.97438051417165	39.0	35.0	40.0	33.0	40.0
33	36.82607939346499	39.0	35.0	40.0	32.0	40.0
34	36.36753153909957	39.0	35.0	40.0	31.0	40.0
35	36.37027019573652	39.0	35.0	40.0	31.0	40.0
36	36.41696100331142	39.0	35.0	40.0	31.0	40.0
37	36.35619507041805	38.0	35.0	40.0	31.0	40.0
38	36.30624799798048	38.0	35.0	40.0	31.0	40.0
39	36.27727466541083	38.0	35.0	40.0	31.0	40.0
40	36.03526099970513	38.0	35.0	40.0	31.0	40.0
41	35.87514398630459	38.0	35.0	40.0	30.0	40.0
42	35.79274033249965	37.0	35.0	40.0	30.0	40.0
43	35.69529421997077	37.0	35.0	40.0	30.0	40.0
44	35.36485440147306	37.0	34.0	40.0	29.0	40.0
45	35.274275083315835	36.0	34.0	40.0	29.0	40.0
46	35.077749422463775	36.0	34.0	40.0	29.0	40.0
47	35.02100768139093	36.0	34.0	40.0	29.0	40.0
48	34.90320089775329	35.0	34.0	40.0	29.0	40.0
49	34.78334277331942	35.0	34.0	39.0	28.0	40.0
50	34.23007685633614	35.0	33.0	39.0	27.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	0.0
15	5.0
16	19.0
17	34.0
18	67.0
19	121.0
20	213.0
21	397.0
22	585.0
23	879.0
24	1431.0
25	2007.0
26	2702.0
27	3470.0
28	4555.0
29	5861.0
30	7754.0
31	10590.0
32	14416.0
33	21984.0
34	48459.0
35	41611.0
36	48119.0
37	76372.0
38	141200.0
39	38547.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	100.0	0.0	0.0	0.0
2	0.0	0.0	0.0	100.0
3	0.0	100.0	0.0	0.0
4	100.0	0.0	0.0	0.0
5	100.0	0.0	0.0	0.0
6	0.0	100.0	0.0	0.0
7	99.19134321455921	0.6603747568408078	0.10882500811414533	0.039457020485830475
8	99.41450872827477	0.45290719751208636	0.08442953845892757	0.04815453575421246
9	98.74522432164684	0.7199845566070356	0.27428993273214414	0.26050118901397756
10	67.9978107717666	25.21685451178301	2.2015320354943477	4.5838026809560475
11	42.41269073544917	24.31146438579632	17.929609523991353	15.346235354763163
12	35.01089310753735	16.752899348534893	24.90544103827119	23.330766505656566
13	27.184190038587268	16.199652523658305	27.69585849779062	28.92029893996381
14	24.98817350058019	16.99706617960581	31.10783009722125	26.906930222592752
15	27.614186708075323	18.35345429243592	28.776896005294876	25.255462994193877
16	33.96973688955641	18.10504477099018	23.915621373825573	24.009596965627843
17	33.11631972066127	21.58914210679276	21.816126041845653	23.478412130700317
18	33.1521704543285	21.137083447355636	22.048201205348334	23.66254489296753
19	31.14049881310737	23.46207777275726	20.75078648872823	24.64663692540714
20	32.53804102257323	23.52656666645453	20.92388825602091	23.011504054951327
21	33.3104228053093	21.603142985029667	22.78664146508584	22.29979274457519
22	30.60698049847369	20.842852869861836	24.231489672230953	24.318676959433517
23	32.25675065072264	20.42982696187306	23.56729649405281	23.74612589335149
24	31.734475465582236	21.238271612795106	22.715364266788857	24.3118886548338
25	30.336296852560146	20.49304304845789	21.629235530834812	27.541424568147153
26	28.06900311625608	21.511288738414805	24.038659394695365	26.381048750633752
27	25.360257446451946	19.707084656522394	23.259065038322102	31.673592858703557
28	27.32907791488739	23.151088568282923	21.330550128447452	28.18928338838224
29	31.044401876117682	22.830341175946494	20.788970702101615	25.33628624583421
30	29.045458306021015	20.565168784829837	24.30128192889675	26.0880909802524
31	32.14813777712723	20.643870691282757	22.08468834257179	25.12330318901822
32	34.39676367578209	21.10462686598826	21.316337115691802	23.18227234253785
33	34.495194092477924	20.02146801329659	22.51532141561607	22.968016478609414
34	30.134132656199945	22.346250204179473	25.647699719346033	21.871917420274546
35	28.09955048695479	26.426657672163074	24.890379487440576	20.583412353441563
36	29.27838200759866	27.662341243829538	21.258848661112985	21.80042808745882
37	30.309567903198776	26.744223046718385	19.690113895023114	23.25609515505973
38	30.006427675917852	27.41244678075261	20.240815105674812	22.340310437654725
39	30.13243558005002	24.221519349850126	21.152993536261214	24.49305153383864
40	30.107615841357322	22.838614422177393	19.135382128515335	27.918387607949953
41	25.776253237703088	25.048419703902642	21.060290751571387	28.115036306822883
42	23.69818349211602	24.162333819121383	22.02826056058668	30.111222128175918
43	25.90989798450994	19.823122238273736	24.691821577898978	29.575158199317354
44	26.920506831792174	20.575563376248144	23.865769761921428	28.638160030038247
45	28.316988368664337	22.367463656053577	22.414981788251566	26.900566187030524
46	28.12097607334763	24.246126954024085	21.899070638673397	25.73382633395489
47	25.238280098175853	23.512777922736365	23.249731119497497	27.999210859590285
48	28.100399025029756	21.104839000507	25.547784361019012	25.246977613444237
49	28.865356099609883	20.80848707782579	24.624999204495555	25.70115761806877
50	25.211763283333227	21.741454691248816	24.870650977197663	28.1761310482203
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	2.0
8	4.0
9	4.5
10	5.0
11	27.0
12	49.0
13	157.0
14	265.0
15	477.5
16	690.0
17	625.0
18	560.0
19	561.5
20	563.0
21	539.0
22	515.0
23	523.5
24	532.0
25	560.0
26	588.0
27	829.5
28	1071.0
29	1321.0
30	1571.0
31	1956.0
32	2341.0
33	2726.5
34	3112.0
35	3881.0
36	4650.0
37	5303.5
38	5957.0
39	6083.5
40	6210.0
41	7134.0
42	8058.0
43	9200.0
44	10342.0
45	9986.0
46	9630.0
47	12175.5
48	14721.0
49	14555.5
50	14390.0
51	15876.5
52	17363.0
53	19533.5
54	21704.0
55	24204.0
56	26704.0
57	29894.0
58	33084.0
59	38823.0
60	44562.0
61	46962.0
62	49362.0
63	47345.5
64	45329.0
65	47141.0
66	48953.0
67	41523.0
68	34093.0
69	27491.0
70	20889.0
71	18012.0
72	15135.0
73	12849.0
74	10563.0
75	8564.5
76	6566.0
77	6715.5
78	6865.0
79	4869.0
80	2873.0
81	1824.0
82	775.0
83	584.5
84	394.0
85	312.0
86	230.0
87	153.5
88	77.0
89	53.0
90	29.0
91	26.5
92	24.0
93	12.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	471399.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	9.666970019028467
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.69431643625192	6.640658974669017
2	9.141979372394118	1.7675048101502124
3	3.37502743032697	0.978788669471085
4	1.6150976519640112	0.6245240231735749
5	0.8865481676541584	0.4285117278568686
6	0.5420232609172702	0.31438335677419765
7	0.40816326530612246	0.2761991434008133
8	0.3445249067368883	0.2664409555387262
9	0.2852754004827737	0.24819738692699816
>10	8.775510204081632	25.055632277539836
>50	4.2089093701996925	28.175918913701558
>100	1.6545973228000876	27.18206869339986
>500	0.04827737546631556	3.311844106584868
>1k	0.017555409260478386	3.257113400749683
>5k	0.0021944261575597983	1.4722135600627069
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GCAGGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	6940	1.4722135600627069	No Hit
GCAGGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG	3913	0.8300823718336271	No Hit
GCAGGAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC	2613	0.5543074974702958	No Hit
GCAGGAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	2361	0.5008495987475577	No Hit
GCAGGAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG	1688	0.3580830676348486	No Hit
GCAGGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTG	1231	0.26113759257020064	No Hit
GCAGGAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT	1216	0.25795557478908526	No Hit
GCAGGAGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCT	1189	0.2522279427830776	No Hit
GCAGGAGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCT	1143	0.2424697549209905	No Hit
GCAGGAGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCG	1000	0.21213451874102407	No Hit
GCAGGAGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGC	999	0.21192238422228304	No Hit
GCAGGAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG	922	0.19558802627922417	No Hit
GCAGGAGGGGATCGTGGTGTGCCCAGCTCTTCCAAGGACTGCTGCGCTTC	844	0.1790415338174243	No Hit
GCAGGAGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACAGCT	831	0.176283785073791	No Hit
GCAGGAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	803	0.17034401854904233	No Hit
GCAGGAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA	772	0.16376784846807058	No Hit
GCAGGAGGGGGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCAT	747	0.15846448549954498	No Hit
GCAGGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTGCTTGCCTAGC	718	0.15231258445605528	No Hit
GCAGGAGGGAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATACCGTC	701	0.14870629763745785	No Hit
GCAGGAGGGGAAAATCCTTTCCTTTATGCCCTGTCCCTTCCCCAGCACTC	680	0.14425147274389635	No Hit
GCAGGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGT	678	0.14382720370641433	No Hit
GCAGGAGGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCC	673	0.14276653111270918	No Hit
GCAGGAGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAA	659	0.13979664785033485	No Hit
GCAGGAGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCG	642	0.13619036103173743	No Hit
GCAGGAGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAG	635	0.13470541940055028	No Hit
GCAGGAGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTC	595	0.1262200386509093	No Hit
GCAGGAGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC	582	0.123462289907276	No Hit
GCAGGAGGGGGGGCTGGAGAGATGGCTCAGAGGTTAAGAGCACTGACTGC	562	0.11921959953245552	No Hit
GCAGGAGGGGAGCCGGGCGTGGTGGCGCATGCCTTTAATCCCAGCACTCG	532	0.11285556397022481	No Hit
GCAGGAGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGC	528	0.11200702589526071	No Hit
GCAGGAGGGGGGTGCTGGAGAGATGGCTCAGCGGTTAAGAGCGTTGACTG	509	0.10797647003918125	No Hit
GCAGGAGGGGGGCTGGAAAGTTGGCTCAGCGGTTAAGAACACTGACTGCT	499	0.10585512485177101	No Hit
GCAGGAGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTC	491	0.10415804870184281	No Hit
GCAGGAGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCG	487	0.10330951062687871	No Hit
GCAGGAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCAT	485	0.10288524158939666	No Hit
GCAGGAGGGGACTGTCCCCATAGTAGAGGCCACAGGAAGACCGTCGTGGA	483	0.10246097255191462	No Hit
GCAGGAGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGT	476	0.10097603092072746	No Hit
GCAGGAGGGGGTCTTCCGGTCGGGCCGAGCTCGTCGCTAGGTTTGTTGAA	476	0.10097603092072746	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.030547370698707464	0.0	0.0	0.0	0.0
7	0.031183774254930536	0.0	0.0	0.0	0.0
8	0.03606286818597409	0.0	0.0	0.0	0.0
9	0.04263903826694584	0.0	0.0	0.0	0.0
10	0.05494284035392523	0.0	0.0	0.0	0.0
11	0.08018684808410709	0.0	0.0	0.0	0.0
12	0.09418772632101469	0.0	0.0	0.0	0.0
13	0.10543085581428896	0.0	0.0	0.0	0.0
14	0.11709825434504528	0.0	0.0	0.0	0.0
15	0.12982632546950673	0.0	0.0	0.0	0.0
16	0.14234226207522716	0.0	0.0	0.0	0.0
17	0.15634314031213473	0.0	0.0	0.0	0.0
18	0.16525279009925775	0.0	0.0	0.0	0.0
19	0.18264782063602172	0.0	0.0	0.0	0.0
20	0.19855790954159852	0.0	0.0	0.0	0.0
21	0.21319519133472917	0.0	0.0	0.0	0.0
22	0.22146843756562912	0.0	0.0	0.0	0.0
23	0.23928773713987514	0.0	0.0	0.0	0.0
24	0.25180367374559554	0.0	0.0	0.0	0.0
25	0.26580455198250313	0.0	0.0	0.0	0.0
26	0.28171464088807996	0.0	0.0	0.0	0.0
27	0.29316990490009526	0.0	0.0	0.0	0.0
28	0.3137469532179746	0.0	0.0	0.0	0.0
29	0.3273235624174001	0.0	0.0	0.0	0.0
30	0.3409001716168257	0.0	0.0	0.0	0.0
31	0.35384037726002815	0.0	0.0	0.0	0.0
32	0.3686897935718998	0.0	0.0	0.0	0.0
33	0.38247853729006637	0.0	0.0	0.0	0.0
34	0.39732795360193807	0.0	0.0	0.0	0.0
35	0.41302590798877387	0.0	0.0	0.0	0.0
36	0.43063307304427884	0.0	0.0	0.0	0.0
37	0.4461188929123736	0.0	0.0	0.0	0.0
38	0.4599076366305402	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATTAC	60	0.0	44.000004	11
GATAACC	30	2.5245263E-6	44.000004	9
CTCAAAT	30	2.5245263E-6	44.000004	24
TAACGAA	30	2.5245263E-6	44.000004	32
TACGACA	30	2.5245263E-6	44.000004	43
CGCTAAG	30	2.5245263E-6	44.000004	44
TCGTAAG	30	2.5245263E-6	44.000004	14
TTAGTCT	30	2.5245263E-6	44.000004	39
CGTAACT	30	2.5245263E-6	44.000004	42
ACGTAAC	30	2.5245263E-6	44.000004	41
ACGCGAT	25	4.438832E-5	44.0	12
AACGTTG	20	7.85175E-4	44.0	22
CTAGATG	40	8.294592E-9	44.0	29
ACAACCG	25	4.438832E-5	44.0	36
AACGTAC	40	8.294592E-9	44.0	43
AGCGTAT	25	4.438832E-5	44.0	20
AATCCGT	35	1.4436591E-7	44.0	24
GCGACGT	20	7.85175E-4	44.0	16
CGTATAT	20	7.85175E-4	44.0	19
AATCACT	25	4.438832E-5	44.0	40
>>END_MODULE