FastQCFastQC Report
Fri 17 Jun 2016
SRR1527857_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527857_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences716193
Sequences flagged as poor quality0
Sequence length52
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAGGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC107091.4952673371563252No Hit
GCAGGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT51160.7143325891205304No Hit
GCAGGAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT36620.5113146875213804No Hit
GCAGGAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG36090.5039144476419065No Hit
GCAGGAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG27220.38006515003637287No Hit
GCAGGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT19810.2766014188912765No Hit
GCAGGAGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCT18620.2599857859543447No Hit
GCAGGAGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCT18020.25160815590211016No Hit
GCAGGAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC16900.23596991313793908No Hit
GCAGGAGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG15750.21991278887115623No Hit
GCAGGAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG15280.21335031199690588No Hit
GCAGGAGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC14420.20134237558870305No Hit
GCAGGAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC13500.18849667617527677No Hit
GCAGGAGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACAGCTCT13490.18835704900773953No Hit
GCAGGAGGGGATCGTGGTGTGCCCAGCTCTTCCAAGGACTGCTGCGCTTCGG12860.1795605374528933No Hit
GCAGGAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT12360.17257917907603118No Hit
GCAGGAGGGAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATACCGTCGT11650.162665650180887No Hit
GCAGGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC10630.1484236790920883No Hit
GCAGGAGGGGAAAATCCTTTCCTTTATGCCCTGTCCCTTCCCCAGCACTCCT10600.14800479758947657No Hit
GCAGGAGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAAAA10520.14688778024917865No Hit
GCAGGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTGCTTGCCTAGCAA10390.14507262707119448No Hit
GCAGGAGGGGGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATGG10310.14395560973089655No Hit
GCAGGAGGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGC10300.1438159825633593No Hit
GCAGGAGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAA10220.14269896522306139No Hit
GCAGGAGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGAT9700.1354383525111248No Hit
GCAGGAGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC9480.13236655482530546No Hit
GCAGGAGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCA8990.1255248236159806No Hit
GCAGGAGGGGGGGCTGGAGAGATGGCTCAGAGGTTAAGAGCACTGACTGCTC8820.1231511617678475No Hit
GCAGGAGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGA8380.11700756639620885No Hit
GCAGGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTT8070.11267912420255433No Hit
GCAGGAGGGGGGCTGGAAAGTTGGCTCAGCGGTTAAGAACACTGACTGCTCT7710.10765254617121363No Hit
GCAGGAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGGCTGC7560.10555813865815498No Hit
GCAGGAGGGGAGTTGCTTCAGTGTCCTGGTGCGGAGCGTCACGTCTCGTGCG7540.10527888432308051No Hit
GCAGGAGGGGGGTGCTGGAGAGATGGCTCAGCGGTTAAGAGCGTTGACTGCT7480.10444112131785706No Hit
GCAGGAGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC7460.10416186698278258No Hit
GCAGGAGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT7250.10122969646450049No Hit
GCAGGAGGGGAGCCGGGCGTGGTGGCGCATGCCTTTAATCCCAGCACTCGGG7230.10095044212942601No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCGTA253.4158573E-546.017
ATTTACG253.4158573E-546.023
AACGTTG206.309853E-446.022
AACGTAT206.309853E-446.045
TGTCACG206.309853E-446.040
AGCGTAC206.309853E-446.025
TCGTTGA301.8604132E-646.027
TCGCACC206.309853E-446.046
GCGACGT253.4158573E-546.016
CGTATCC206.309853E-446.033
CTCAAAT301.8604132E-646.024
ACGTTTT206.309853E-446.019
TTAGCAC501.6370905E-1146.030
ACTCTCG206.309853E-446.021
CAACCCG206.309853E-446.018
GCGCCTA206.309853E-446.027
GTTAGCC206.309853E-446.028
CGTCTAC206.309853E-446.012
TAAGTCG206.309853E-446.030
TAACGTC206.309853E-446.029
Produced by FastQC (version 0.11.5)