FastQCFastQC Report
Fri 17 Jun 2016
SRR1527856_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527856_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences723581
Sequences flagged as poor quality0
Sequence length52
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAGGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC100911.3945916213941494No Hit
GCAGGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT50210.6939098732553785No Hit
GCAGGAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT36010.4976637031652296No Hit
GCAGGAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG33510.4631133211071048No Hit
GCAGGAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG25140.347438641976503No Hit
GCAGGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT18770.259404268492401No Hit
GCAGGAGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCT17650.24392569733036107No Hit
GCAGGAGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCT17260.23853583772929363No Hit
GCAGGAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC15740.21752920543795373No Hit
GCAGGAGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG14510.20053041746535633No Hit
GCAGGAGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC14280.19735178231600883No Hit
GCAGGAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG14010.19362034105373135No Hit
GCAGGAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC13930.19251472882787138No Hit
GCAGGAGGGGATCGTGGTGTGCCCAGCTCTTCCAAGGACTGCTGCGCTTCGG13190.18228781573866645No Hit
GCAGGAGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACAGCTCT12270.1695732751412765No Hit
GCAGGAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT11960.16528902776606905No Hit
GCAGGAGGGGGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATGG10780.14898124743463415No Hit
GCAGGAGGGAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATACCGTCGT10660.14732282909584415No Hit
GCAGGAGGGGAAAATCCTTTCCTTTATGCCCTGTCCCTTCCCCAGCACTCCT10470.14469700005942665No Hit
GCAGGAGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAAAA10380.14345318630533416No Hit
GCAGGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC10200.14096555879714917No Hit
GCAGGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTGCTTGCCTAGCAA10090.1394453419865917No Hit
GCAGGAGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGAT9710.13419368391375672No Hit
GCAGGAGGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGC9420.13018583959501423No Hit
GCAGGAGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAA8820.12189374790106428No Hit
GCAGGAGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCA8650.1195443219211118No Hit
GCAGGAGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC8550.11816230663878682No Hit
GCAGGAGGGGGGGCTGGAGAGATGGCTCAGAGGTTAAGAGCACTGACTGCTC8380.11581288065883433No Hit
GCAGGAGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGA8170.11291064856595184No Hit
GCAGGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTT7950.10987021494483687No Hit
GCAGGAGGGGAGTTGCTTCAGTGTCCTGGTGCGGAGCGTCACGTCTCGTGCG7570.10461855687200189No Hit
GCAGGAGGGGGGTGCTGGAGAGATGGCTCAGCGGTTAAGAGCGTTGACTGCT7270.10047251102502691No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTTCGC351.0188705E-746.00000445
GCGAGAC253.415892E-546.030
CTTAACG206.309897E-446.015
GGAATCG253.415892E-546.010
AACCGGC253.415892E-546.026
GATACGA253.415892E-546.015
CTCACAT206.309897E-446.043
TACCCGC253.415892E-546.012
CATATGA253.415892E-546.019
GATCGTT206.309897E-446.09
AATCACT206.309897E-446.040
CTCAAAT301.8604405E-646.024
ACGTTTC206.309897E-446.046
AGATACG301.8604405E-646.038
CATGCGG253.415892E-546.034
AACCCGT206.309897E-446.045
TAGACCC405.6061253E-946.015
GCGAACC405.6061253E-946.027
TAGCGTA206.309897E-446.019
TTAGACG206.309897E-446.043
Produced by FastQC (version 0.11.5)