##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527856_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	723581
Sequences flagged as poor quality	0
Sequence length	52
%GC	59
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.971393665671155	31.0	31.0	34.0	31.0	34.0
2	32.50401682741808	34.0	31.0	34.0	31.0	34.0
3	32.63984819944139	34.0	31.0	34.0	31.0	34.0
4	36.282171588253426	37.0	37.0	37.0	35.0	37.0
5	36.29075804920251	37.0	37.0	37.0	35.0	37.0
6	35.10487008365339	37.0	35.0	37.0	32.0	37.0
7	36.16454964959002	37.0	35.0	37.0	35.0	37.0
8	36.53527801310427	37.0	37.0	37.0	35.0	37.0
9	38.62996264412692	39.0	39.0	39.0	38.0	39.0
10	37.90763715465165	39.0	38.0	39.0	35.0	39.0
11	37.50372245816294	39.0	37.0	39.0	35.0	39.0
12	36.7077244980175	39.0	35.0	39.0	33.0	39.0
13	36.41044333668242	39.0	35.0	39.0	33.0	39.0
14	37.31494469865848	40.0	36.0	40.0	33.0	40.0
15	37.45891061263355	40.0	36.0	40.0	33.0	40.0
16	37.518815447061215	40.0	36.0	40.0	33.0	40.0
17	37.3827187281037	40.0	36.0	40.0	33.0	40.0
18	37.392504778317836	40.0	36.0	40.0	33.0	40.0
19	37.33117646814939	40.0	35.0	40.0	33.0	40.0
20	37.23172664843328	39.0	35.0	40.0	32.0	40.0
21	37.29245792799977	39.0	35.0	40.0	33.0	40.0
22	37.17354656907796	39.0	35.0	40.0	33.0	40.0
23	37.22094416520058	39.0	35.0	40.0	33.0	40.0
24	37.197765004885426	39.0	35.0	40.0	33.0	40.0
25	37.16049896279753	39.0	35.0	40.0	33.0	40.0
26	36.950617829931964	39.0	35.0	40.0	32.0	40.0
27	36.894488661255615	39.0	35.0	40.0	32.0	40.0
28	36.74320497636063	39.0	35.0	40.0	32.0	40.0
29	36.68209226057622	38.0	35.0	40.0	32.0	40.0
30	36.51177822524362	38.0	35.0	40.0	31.0	40.0
31	36.37757072117704	38.0	35.0	40.0	31.0	40.0
32	36.26431595080578	38.0	35.0	40.0	31.0	40.0
33	35.89539940932667	38.0	35.0	40.0	30.0	40.0
34	35.66955738196553	38.0	34.0	40.0	29.0	40.0
35	35.63084160584648	38.0	34.0	40.0	29.0	40.0
36	35.57703975090556	38.0	34.0	40.0	29.0	40.0
37	35.58243513856776	38.0	34.0	40.0	29.0	40.0
38	35.233304909885696	37.0	34.0	40.0	29.0	40.0
39	35.359440062688215	37.0	34.0	40.0	29.0	40.0
40	35.168691549391156	37.0	34.0	40.0	29.0	40.0
41	34.811335565748685	36.0	33.0	40.0	27.0	40.0
42	34.8374501265235	36.0	33.0	40.0	28.0	40.0
43	34.886547601443375	36.0	33.0	40.0	28.0	40.0
44	34.709845891475865	36.0	33.0	39.0	28.0	40.0
45	34.526471811725294	35.0	33.0	39.0	27.0	40.0
46	34.13647815517544	35.0	33.0	39.0	26.0	40.0
47	33.898958098678655	35.0	33.0	39.0	25.0	40.0
48	33.832045064754325	35.0	33.0	39.0	26.0	40.0
49	33.777427820796845	35.0	32.0	38.0	26.0	40.0
50	33.61647693900199	35.0	32.0	38.0	26.0	40.0
51	34.08728255717052	35.0	33.0	39.0	27.0	40.0
52	33.87830940834544	35.0	33.0	38.0	27.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	8.0
16	18.0
17	54.0
18	130.0
19	229.0
20	486.0
21	784.0
22	1321.0
23	1963.0
24	2850.0
25	3963.0
26	5337.0
27	7110.0
28	9202.0
29	11854.0
30	15298.0
31	19957.0
32	26945.0
33	41106.0
34	76583.0
35	68973.0
36	87324.0
37	125284.0
38	179423.0
39	37378.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	100.0	0.0	0.0	0.0
2	0.0	0.0	0.0	100.0
3	0.0	100.0	0.0	0.0
4	100.0	0.0	0.0	0.0
5	100.0	0.0	0.0	0.0
6	0.0	100.0	0.0	0.0
7	99.28356327764273	0.5989654233596515	0.09494444989572695	0.022526849101897367
8	99.4811914630152	0.4035484624388977	0.07186479468089958	0.04339527986500475
9	98.76364912843206	0.7355085332533607	0.2791670870296484	0.2216752512849287
10	68.13224780639624	24.994299186960408	2.2720331241422866	4.601419882501061
11	42.4701588350164	24.2887803853335	17.75972558704554	15.48133519260456
12	34.8978207001013	16.667104304839402	24.97177233785851	23.46330265720078
13	27.31926349641574	16.200535945526486	27.69793568377279	28.78226487428498
14	25.101819975925295	16.972667883761456	30.9115358197631	27.013976320550153
15	27.805732875794142	18.42654796076735	28.727398867576674	25.040320295861836
16	34.10495853263145	18.096660912876374	23.845429882763643	23.95295067172853
17	33.11819962105141	21.479834324007953	21.6879658255261	23.714000229414538
18	33.064162823512504	21.150776485286375	22.17070376364222	23.614356927558905
19	31.258836260211364	23.33988869248916	20.77901437434095	24.62226067295852
20	32.4798467621455	23.55810890556828	20.991291921706072	22.970752410580157
21	33.49866842827548	21.48135454081851	22.70222684122441	22.317750189681597
22	30.67313818356203	20.794216542446524	24.176146139824013	24.356499134167425
23	32.246147977904336	20.405455643528505	23.52894838311122	23.819447995455935
24	31.770596519256312	20.948864052538692	22.673204520295588	24.60733490790941
25	30.143688128903328	20.63943083082613	21.544512639220763	27.67236840104978
26	28.288747216966726	21.332649696440342	23.981005581959725	26.397597504633207
27	25.44663278886538	19.557865670878588	23.407054635210155	31.588446905045874
28	27.495885049496877	23.041096988450498	21.25788266966656	28.205135292386064
29	31.05457440148373	22.756401840291552	20.895932867225646	25.29309089099907
30	29.273018501038585	20.41886119176706	24.324989185730416	25.98313112146394
31	32.16046303040019	20.431437530836217	22.134633164773536	25.27346627399006
32	34.515831676066675	21.010363732602155	21.231209774717687	23.242594816613483
33	34.59059870284046	19.914978419831368	22.558220848806148	22.936202028522032
34	30.04183360259598	22.219627104636523	25.722206636160983	22.016332656606515
35	28.0449597211646	26.37465605094661	24.940953397062664	20.63943083082613
36	29.518049810594803	27.65771903905713	21.071587009609154	21.75264414073891
37	30.456714590349943	26.58527517997294	19.669532505690448	23.288477723986674
38	30.21721134192302	27.031804317692142	20.280659663534557	22.470324676850275
39	30.044321230104167	24.0072638723239	21.213934583688626	24.73448031388331
40	30.155020654218394	22.63741032448337	19.126953305849657	28.08061571544858
41	26.141095468233686	24.958781394204657	20.943059588352927	27.957063549208726
42	23.753111261904333	24.262522094969327	21.873017671829416	30.111348971296927
43	25.90946970691602	19.54363091347064	24.83840786311415	29.70849151649919
44	26.814413313782424	20.427015081932776	23.935122674586534	28.823448929698266
45	28.53239651124062	22.20290471972039	22.134909567830004	27.129789201208986
46	27.952641100305286	24.279106278357226	21.990765373883505	25.777487247453983
47	25.614824048724333	23.138390864326176	23.362139138534594	27.8846459484149
48	28.19794881291797	20.961716794664316	25.51255491783228	25.327779474585434
49	28.934148353812496	20.67660704192067	24.619634843922103	25.769609760344732
50	25.141622016056253	21.65866710154081	24.882908755205015	28.31680212719792
51	24.501057932698618	21.332096890327414	26.070474487306882	28.09637068966709
52	25.84714081768316	22.311531120911134	25.123379414329566	26.71794864707614
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	2.5
8	5.0
9	4.0
10	3.0
11	29.0
12	55.0
13	178.0
14	606.5
15	912.0
16	842.0
17	772.0
18	787.5
19	803.0
20	732.5
21	662.0
22	661.0
23	660.0
24	697.5
25	735.0
26	943.0
27	1151.0
28	1543.5
29	1936.0
30	2361.5
31	2787.0
32	3208.0
33	3629.0
34	4641.5
35	5654.0
36	6417.0
37	7180.0
38	7909.0
39	9392.0
40	10146.0
41	11796.5
42	13447.0
43	13399.0
44	13351.0
45	15658.5
46	17966.0
47	19554.0
48	21142.0
49	20611.5
50	20081.0
51	22973.0
52	25865.0
53	28646.0
54	31427.0
55	36048.0
56	40669.0
57	45877.5
58	51086.0
59	55394.0
60	59702.0
61	69467.0
62	79232.0
63	71661.5
64	69217.0
65	74343.0
66	61685.0
67	49027.0
68	44698.5
69	40370.0
70	33192.5
71	26015.0
72	22494.0
73	18973.0
74	15149.0
75	11325.0
76	10895.5
77	10466.0
78	7997.0
79	5528.0
80	3646.0
81	1764.0
82	1385.0
83	1006.0
84	754.5
85	503.0
86	306.5
87	110.0
88	82.0
89	29.5
90	5.0
91	3.5
92	2.0
93	2.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	723581.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	11.677476329533253
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.89343874266238	8.628888818252552
2	10.153143344063624	2.3712618214132215
3	3.440399545540617	1.2052555277156254
4	1.713690588903617	0.8004632515226354
5	0.9207536451429653	0.5376039448244219
6	0.5550558606324559	0.3888991004462527
7	0.349128952849839	0.28538615580011084
8	0.23196364324938457	0.21669999626855874
9	0.18817458814618443	0.19776638690070636
>10	3.514959288013634	11.848569821485086
>50	3.085353152811967	25.809273598947456
>100	1.8639935618254118	36.459359767600304
>500	0.06390835069115698	4.728427086946727
>1k	0.023669759515243326	4.433643227226806
>5k	0.0011834879757621662	0.6939098732553785
>10k+	0.0011834879757621662	1.3945916213941494
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GCAGGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	10091	1.3945916213941494	No Hit
GCAGGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	5021	0.6939098732553785	No Hit
GCAGGAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	3601	0.4976637031652296	No Hit
GCAGGAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	3351	0.4631133211071048	No Hit
GCAGGAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG	2514	0.347438641976503	No Hit
GCAGGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT	1877	0.259404268492401	No Hit
GCAGGAGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCT	1765	0.24392569733036107	No Hit
GCAGGAGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCT	1726	0.23853583772929363	No Hit
GCAGGAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	1574	0.21752920543795373	No Hit
GCAGGAGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG	1451	0.20053041746535633	No Hit
GCAGGAGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC	1428	0.19735178231600883	No Hit
GCAGGAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	1401	0.19362034105373135	No Hit
GCAGGAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	1393	0.19251472882787138	No Hit
GCAGGAGGGGATCGTGGTGTGCCCAGCTCTTCCAAGGACTGCTGCGCTTCGG	1319	0.18228781573866645	No Hit
GCAGGAGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACAGCTCT	1227	0.1695732751412765	No Hit
GCAGGAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT	1196	0.16528902776606905	No Hit
GCAGGAGGGGGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATGG	1078	0.14898124743463415	No Hit
GCAGGAGGGAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATACCGTCGT	1066	0.14732282909584415	No Hit
GCAGGAGGGGAAAATCCTTTCCTTTATGCCCTGTCCCTTCCCCAGCACTCCT	1047	0.14469700005942665	No Hit
GCAGGAGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAAAA	1038	0.14345318630533416	No Hit
GCAGGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC	1020	0.14096555879714917	No Hit
GCAGGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTGCTTGCCTAGCAA	1009	0.1394453419865917	No Hit
GCAGGAGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGAT	971	0.13419368391375672	No Hit
GCAGGAGGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGC	942	0.13018583959501423	No Hit
GCAGGAGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAA	882	0.12189374790106428	No Hit
GCAGGAGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCA	865	0.1195443219211118	No Hit
GCAGGAGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	855	0.11816230663878682	No Hit
GCAGGAGGGGGGGCTGGAGAGATGGCTCAGAGGTTAAGAGCACTGACTGCTC	838	0.11581288065883433	No Hit
GCAGGAGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGA	817	0.11291064856595184	No Hit
GCAGGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTT	795	0.10987021494483687	No Hit
GCAGGAGGGGAGTTGCTTCAGTGTCCTGGTGCGGAGCGTCACGTCTCGTGCG	757	0.10461855687200189	No Hit
GCAGGAGGGGGGTGCTGGAGAGATGGCTCAGCGGTTAAGAGCGTTGACTGCT	727	0.10047251102502691	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.023356058271292363	0.0	0.0	0.0	0.0
7	0.023494259799524863	0.0	0.0	0.0	0.0
8	0.029298723985289828	0.0	0.0	0.0	0.0
9	0.03565599428398479	0.0	0.0	0.0	0.0
10	0.05030535627662971	0.0	0.0	0.0	0.0
11	0.07518163135847956	0.0	0.0	0.0	0.0
12	0.08457933527828951	0.0	0.0	0.0	0.0
13	0.09480624836749445	0.0	0.0	0.0	0.0
14	0.10890280424720937	0.0	0.0	0.0	0.0
15	0.12258475554222678	0.0	0.0	0.0	0.0
16	0.1335026762725942	0.0	0.0	0.0	0.0
17	0.14372958936179916	0.0	0.0	0.0	0.0
18	0.1536800993945391	0.0	0.0	0.0	0.0
19	0.16708564763309153	0.0	0.0	0.0	0.0
20	0.17938558364578394	0.0	0.0	0.0	0.0
21	0.19251472882787138	0.0	0.0	0.0	0.0
22	0.2030180449735413	0.0	0.0	0.0	0.0
23	0.21476517487330374	0.0	0.0	1.382015282324992E-4	0.0
24	0.22720331241422867	0.0	0.0	1.382015282324992E-4	0.0
25	0.2388122407857586	0.0	0.0	1.382015282324992E-4	0.0
26	0.253323401250171	0.0	0.0	1.382015282324992E-4	0.0
27	0.26493232962170093	0.0	0.0	1.382015282324992E-4	0.0
28	0.27902888550141586	0.0	0.0	1.382015282324992E-4	0.0
29	0.2895322016470858	0.0	0.0	1.382015282324992E-4	0.0
30	0.30210854071624327	0.0	0.0	1.382015282324992E-4	0.0
31	0.31205905074898316	0.0	0.0	1.382015282324992E-4	0.0
32	0.3256028005157681	0.0	0.0	1.382015282324992E-4	0.0
33	0.33569151207674053	0.0	0.0	1.382015282324992E-4	0.0
34	0.34647123127887547	0.0	0.0	1.382015282324992E-4	0.0
35	0.3611205932715204	0.0	0.0	1.382015282324992E-4	0.0
36	0.3796395980546753	0.0	0.0	1.382015282324992E-4	0.0
37	0.3938743554626227	0.0	0.0	1.382015282324992E-4	0.0
38	0.40244285021303766	0.0	0.0	1.382015282324992E-4	0.0
39	0.41294616635870757	0.0	0.0	1.382015282324992E-4	0.0
40	0.427733729879585	0.0	0.0	1.382015282324992E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTTCGC	35	1.0188705E-7	46.000004	45
GCGAGAC	25	3.415892E-5	46.0	30
CTTAACG	20	6.309897E-4	46.0	15
GGAATCG	25	3.415892E-5	46.0	10
AACCGGC	25	3.415892E-5	46.0	26
GATACGA	25	3.415892E-5	46.0	15
CTCACAT	20	6.309897E-4	46.0	43
TACCCGC	25	3.415892E-5	46.0	12
CATATGA	25	3.415892E-5	46.0	19
GATCGTT	20	6.309897E-4	46.0	9
AATCACT	20	6.309897E-4	46.0	40
CTCAAAT	30	1.8604405E-6	46.0	24
ACGTTTC	20	6.309897E-4	46.0	46
AGATACG	30	1.8604405E-6	46.0	38
CATGCGG	25	3.415892E-5	46.0	34
AACCCGT	20	6.309897E-4	46.0	45
TAGACCC	40	5.6061253E-9	46.0	15
GCGAACC	40	5.6061253E-9	46.0	27
TAGCGTA	20	6.309897E-4	46.0	19
TTAGACG	20	6.309897E-4	46.0	43
>>END_MODULE