FastQCFastQC Report
Fri 17 Jun 2016
SRR1527855_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527855_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1336604
Sequences flagged as poor quality0
Sequence length50
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC147121.1006999829418436No Hit
AGATGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG67290.5034400615290692No Hit
AGATGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC43180.32305753985473634No Hit
AGATGGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC40730.3047275034340762No Hit
AGATGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC40340.3018096609018079No Hit
AGATGGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT37980.28415297275782503No Hit
AGATGGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG32980.24674473516464115No Hit
AGATGGGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGC24030.17978398987284192No Hit
AGATGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTG23760.17776394504280998No Hit
AGATGGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC23520.17596834963833716No Hit
AGATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT20750.15524418601171325No Hit
AGATGGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA19320.14454543006006268No Hit
AGATGGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAA18410.1377371308181032No Hit
AGATGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG18100.13541782008732578No Hit
AGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGT14780.11057875032545167No Hit
AGATGGGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT14310.10706237599169238No Hit
AGATGGGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGT14080.10534159706240592No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTTA207.856941E-444.00000426
TCGTTAA207.856941E-444.00000429
TTCGTAA207.856941E-444.00000439
CGATTAT408.314601E-944.00000414
TCGATAA207.856941E-444.00000433
ACGATTT408.314601E-944.00000432
CTATTCG408.314601E-944.00000411
CACGTAC207.856941E-444.00000417
CGCGTAG207.856941E-444.00000441
TATCGCC408.314601E-944.00000428
ACGGTAT207.856941E-444.00000424
AAGCGTA254.44323E-544.010
TAACGCG254.44323E-544.035
TACGCTT254.44323E-544.037
CGGATAA351.4463149E-744.031
ATACGCC254.44323E-544.041
GTACGTC254.44323E-544.018
AGATGGG1347200.042.9467051
GATGGGG1311550.042.9231072
ATGGGGG1193700.042.923
Produced by FastQC (version 0.11.5)