##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527855_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1336604
Sequences flagged as poor quality	0
Sequence length	50
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.577507623798823	31.0	31.0	34.0	3.0	34.0
2	30.152205140789643	33.0	31.0	34.0	16.0	34.0
3	31.414807227870035	31.0	31.0	34.0	28.0	34.0
4	36.10337616825926	37.0	35.0	37.0	35.0	37.0
5	36.22815358924558	37.0	35.0	37.0	35.0	37.0
6	36.51767539226278	37.0	37.0	37.0	35.0	37.0
7	36.65938004076001	37.0	37.0	37.0	35.0	37.0
8	36.68343952285045	37.0	37.0	37.0	35.0	37.0
9	38.56910274097638	39.0	39.0	39.0	38.0	39.0
10	36.237050764474745	35.0	35.0	39.0	33.0	39.0
11	35.77477397942846	35.0	35.0	39.0	33.0	39.0
12	35.584648108190606	35.0	35.0	39.0	33.0	39.0
13	35.68046257530278	35.0	35.0	39.0	33.0	39.0
14	36.31119015056068	36.0	35.0	40.0	33.0	40.0
15	36.42836921032707	36.0	35.0	40.0	33.0	40.0
16	36.51769110372257	36.0	35.0	40.0	33.0	40.0
17	36.34910489569087	36.0	35.0	40.0	33.0	40.0
18	36.271696777804046	36.0	35.0	40.0	33.0	40.0
19	36.24713901798887	36.0	35.0	40.0	33.0	40.0
20	36.11288683858495	35.0	35.0	40.0	32.0	40.0
21	36.11458442440693	35.0	35.0	40.0	32.0	40.0
22	36.158371514674506	35.0	35.0	40.0	32.0	40.0
23	36.11507821314316	35.0	35.0	40.0	33.0	40.0
24	36.04559839713183	35.0	35.0	40.0	33.0	40.0
25	35.98611855119392	35.0	35.0	40.0	33.0	40.0
26	35.91233229887087	35.0	35.0	40.0	32.0	40.0
27	35.79218601769858	35.0	35.0	40.0	32.0	40.0
28	35.64967185493983	35.0	35.0	40.0	31.0	40.0
29	35.63613231742536	35.0	35.0	40.0	32.0	40.0
30	35.78783244700749	35.0	35.0	40.0	32.0	40.0
31	35.82960398143354	35.0	35.0	40.0	32.0	40.0
32	35.846295537047624	35.0	35.0	40.0	33.0	40.0
33	35.74179338083681	35.0	35.0	40.0	32.0	40.0
34	35.33658286223893	35.0	35.0	40.0	31.0	40.0
35	35.33005362844941	35.0	35.0	40.0	31.0	40.0
36	35.36245365119362	35.0	35.0	40.0	31.0	40.0
37	35.27240080083555	35.0	35.0	40.0	31.0	40.0
38	35.18286193966201	35.0	35.0	40.0	31.0	40.0
39	35.054903322150764	35.0	35.0	40.0	31.0	40.0
40	34.90612627225416	35.0	35.0	40.0	30.0	40.0
41	34.82573522150166	35.0	34.0	40.0	30.0	40.0
42	34.76430116923187	35.0	34.0	40.0	30.0	40.0
43	34.68749906479406	35.0	34.0	40.0	30.0	40.0
44	34.37373672381648	35.0	34.0	40.0	28.0	40.0
45	34.43338864764732	35.0	34.0	39.0	29.0	40.0
46	34.25251158907201	35.0	34.0	39.0	28.0	40.0
47	34.24189438307831	35.0	34.0	39.0	28.0	40.0
48	34.18213921251171	35.0	34.0	39.0	28.0	40.0
49	34.13975867197764	35.0	34.0	39.0	28.0	40.0
50	33.64242513115328	35.0	33.0	38.0	26.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	1.0
12	2.0
13	5.0
14	15.0
15	44.0
16	104.0
17	204.0
18	378.0
19	774.0
20	1221.0
21	1945.0
22	3191.0
23	4828.0
24	6940.0
25	9181.0
26	12215.0
27	16318.0
28	19819.0
29	22984.0
30	27675.0
31	38650.0
32	57965.0
33	122722.0
34	250630.0
35	168772.0
36	68163.0
37	143160.0
38	230264.0
39	128432.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	100.0	0.0	0.0	0.0
3	0.0	100.0	0.0	0.0
4	0.0	0.0	100.0	0.0
5	100.0	0.0	0.0	0.0
6	100.0	0.0	0.0	0.0
7	98.3568057554818	1.2690370521111711	0.21853892401938046	0.15561826838764511
8	96.93409566333783	2.128229453151419	0.6316006835233173	0.30607419998743085
9	90.27385822577219	5.323341842460445	2.8269405149169087	1.5758594168504658
10	51.43146361974078	33.49705671986617	7.084521668347543	7.986957992045513
11	35.99039057192706	23.993868041693727	21.302046080963397	18.713695305415815
12	30.783986880182912	19.280504921427738	25.415829969085834	24.51967822930352
13	25.81879150443961	18.425053344146807	27.70042585537676	28.055729296036823
14	25.084243351059847	19.98864285906671	29.783241708090053	25.143872081783385
15	27.173268971213616	21.262094083213874	26.82896355240595	24.735673393166564
16	31.713656400848716	21.89661260926946	22.993272502551243	23.39645848733058
17	30.31705725854479	22.363467414432396	23.237697926985103	24.08177740003771
18	30.423221836834248	22.04040987457766	23.35560869187882	24.180759596709272
19	29.45584481267451	24.289093852779136	21.003229079069044	25.251832255477314
20	30.955241791884507	23.803086029968487	22.162136279705884	23.079535898441126
21	30.487564005494523	23.291416156168918	23.422719070120994	22.79830076821557
22	28.614084650352684	21.556048014221115	25.492217590251116	24.337649745175085
23	29.13570511535204	21.837881676248163	24.343410613764433	24.683002594635358
24	30.703110270506446	22.694081418280955	22.639091309018976	23.96371700219362
25	28.490637466295176	23.14290545292398	21.414495243168506	26.951961837612338
26	27.334947374091357	23.32141756271865	23.168866769813647	26.17476829337635
27	26.804274115594446	21.41172703358661	23.02499468803026	28.75900416278868
28	28.105257802610197	24.16617038404793	21.48946135130525	26.239110462036624
29	30.490332215076414	22.60295495150396	21.609317344553812	25.297395488865813
30	28.052886269979737	21.751992362734214	23.343937321749745	26.851184045536296
31	30.367857645196334	23.054173113352945	22.603628299780638	23.974340941670082
32	33.29228402728108	21.541907700410892	21.288279849529104	23.877528422778923
33	31.574048858150956	22.854562757555716	22.00771507492122	23.56367330937211
34	27.922855236105832	24.71158248815655	25.417999646866235	21.947562628871378
35	27.72309524735823	26.260732423365486	24.32410796316635	21.692064366109932
36	27.928840554120743	27.318487749550354	21.375889941972343	23.376781754356564
37	30.326858216794207	25.74711732121107	20.657576963708024	23.268447498286704
38	30.668170976594418	25.480845486022787	20.856738420654136	22.994245116728663
39	30.680066796149045	23.919500465358475	20.68645612312996	24.713976615362515
40	28.792447127196986	23.119936795041763	22.255806506639214	25.83180957112204
41	26.946425418448545	23.82568060547477	22.609089902469243	26.61880407360744
42	26.538002280406165	22.562030339577017	23.108190608437503	27.79177677157932
43	25.85141148762087	21.106176548925486	24.53194813123408	28.510463832219568
44	26.145290602152922	21.000236420061587	25.00905279349755	27.84542018428794
45	27.820655931001255	21.691241384882883	23.76926898318425	26.718833700931615
46	27.511289806105623	24.022896834066035	22.428857013745283	26.036956346083056
47	25.09636362004004	23.843112844193193	24.469326741503092	26.591196794263674
48	26.95046550810861	22.63310599100407	25.38956938629542	25.026859114591904
49	27.60974828745088	23.16445259777765	23.84977151048478	25.376027604286683
50	25.840114199867724	22.588814637693737	24.1350467303704	27.436024432068134
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	12.0
6	24.0
7	132.5
8	241.0
9	535.5
10	830.0
11	1145.0
12	1460.0
13	1734.0
14	2008.0
15	2431.5
16	2855.0
17	2476.0
18	2097.0
19	2317.5
20	2538.0
21	3086.5
22	3635.0
23	4413.5
24	5192.0
25	6320.5
26	7449.0
27	8314.5
28	9180.0
29	9440.0
30	9700.0
31	11149.5
32	12599.0
33	14783.0
34	16967.0
35	19075.0
36	21183.0
37	21918.5
38	22654.0
39	26404.0
40	30154.0
41	31450.5
42	32747.0
43	35136.0
44	37525.0
45	40941.0
46	44357.0
47	45353.5
48	46350.0
49	51527.0
50	56704.0
51	59879.5
52	63055.0
53	70726.0
54	78397.0
55	83064.0
56	87731.0
57	101093.5
58	114456.0
59	119561.0
60	124666.0
61	123407.0
62	122148.0
63	120665.0
64	119182.0
65	101406.0
66	83630.0
67	71486.0
68	59342.0
69	48628.0
70	37914.0
71	33018.0
72	28122.0
73	21834.5
74	15547.0
75	18426.5
76	21306.0
77	14697.5
78	8089.0
79	5602.5
80	3116.0
81	2016.5
82	917.0
83	669.5
84	422.0
85	248.5
86	75.0
87	45.5
88	16.0
89	14.5
90	13.0
91	9.0
92	5.0
93	5.5
94	6.0
95	3.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1336604.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	9.846538863531034
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.47228466184497	6.8406155086114735
2	9.134101791276086	1.7987857654249686
3	3.6822616535951154	1.0877259743344307
4	1.7621938599820828	0.6940604130955738
5	1.0154656529340367	0.4999411008097954
6	0.6431132128312476	0.3799463546275911
7	0.44957922828782904	0.30987595405006796
8	0.3591272244583631	0.2828928138065044
9	0.30736254557557025	0.2723811525183313
>10	7.311622949713259	20.99023603392522
>50	4.041932521979817	27.605968531325985
>100	1.735235456035279	29.42331127360439
>500	0.06409636976009263	4.314858322618753
>1k	0.020078380888703715	3.869039054920916
>5k	7.722454187962968E-4	0.5116694273134477
>10k+	7.722454187962968E-4	1.118692319012549
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	14712	1.1006999829418436	No Hit
AGATGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG	6729	0.5034400615290692	No Hit
AGATGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC	4318	0.32305753985473634	No Hit
AGATGGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC	4073	0.3047275034340762	No Hit
AGATGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC	4034	0.3018096609018079	No Hit
AGATGGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	3798	0.28415297275782503	No Hit
AGATGGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG	3298	0.24674473516464115	No Hit
AGATGGGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGC	2403	0.17978398987284192	No Hit
AGATGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTG	2376	0.17776394504280998	No Hit
AGATGGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC	2352	0.17596834963833716	No Hit
AGATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	2075	0.15524418601171325	No Hit
AGATGGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA	1932	0.14454543006006268	No Hit
AGATGGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAA	1841	0.1377371308181032	No Hit
AGATGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG	1810	0.13541782008732578	No Hit
AGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGT	1478	0.11057875032545167	No Hit
AGATGGGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT	1431	0.10706237599169238	No Hit
AGATGGGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGT	1408	0.10534159706240592	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.03651043989094751	0.0	0.0	0.0	0.0
8	0.08790935834398221	0.0	0.0	0.0	0.0
9	0.11880856259595213	0.0	0.0	0.0	0.0
10	0.15090483045090394	0.0	0.0	0.0	0.0
11	0.17222752587901877	0.0	0.0	0.0	0.0
12	0.18621820673886955	0.0	0.0	0.0	0.0
13	0.20088223587539766	0.0	0.0	0.0	0.0
14	0.21996043704792145	0.0	0.0	0.0	0.0
15	0.24158239837678175	0.0	0.0	0.0	0.0
16	0.2614087643011692	0.0	0.0	0.0	0.0
17	0.2816840290766749	0.0	0.0	0.0	0.0
18	0.304278604582958	0.0	0.0	0.0	0.0
19	0.33929271497017816	0.0	0.0	0.0	0.0
20	0.3656281142357796	0.0	0.0	0.0	0.0
21	0.391739064075822	0.0	0.0	0.0	0.0
22	0.4159796020362052	0.0	0.0	0.0	0.0
23	0.44171646950031573	0.0	0.0	0.0	0.0
24	0.46835113466666267	0.0	0.0	0.0	0.0
25	0.49326502090372315	0.0	0.0	0.0	0.0
26	0.5163084952611244	0.0	0.0	0.0	0.0
27	0.5346385316817845	0.0	0.0	0.0	0.0
28	0.5555123282587812	0.0	0.0	0.0	0.0
29	0.5689792937923274	0.0	0.0	0.0	0.0
30	0.584541120631092	0.0	0.0	0.0	0.0
31	0.5980080861646382	0.0	0.0	0.0	0.0
32	0.6114750516981844	0.0	0.0	0.0	0.0
33	0.6241190360046805	0.0	0.0	0.0	0.0
34	0.6384837992404632	0.0	0.0	0.0	0.0
35	0.6538959931288549	0.0	0.0	0.0	0.0
36	0.6697570858683649	0.0	0.0	0.0	0.0
37	0.6839722161537748	0.0	0.0	0.0	0.0
38	0.6966910169354573	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCGTTA	20	7.856941E-4	44.000004	26
TCGTTAA	20	7.856941E-4	44.000004	29
TTCGTAA	20	7.856941E-4	44.000004	39
CGATTAT	40	8.314601E-9	44.000004	14
TCGATAA	20	7.856941E-4	44.000004	33
ACGATTT	40	8.314601E-9	44.000004	32
CTATTCG	40	8.314601E-9	44.000004	11
CACGTAC	20	7.856941E-4	44.000004	17
CGCGTAG	20	7.856941E-4	44.000004	41
TATCGCC	40	8.314601E-9	44.000004	28
ACGGTAT	20	7.856941E-4	44.000004	24
AAGCGTA	25	4.44323E-5	44.0	10
TAACGCG	25	4.44323E-5	44.0	35
TACGCTT	25	4.44323E-5	44.0	37
CGGATAA	35	1.4463149E-7	44.0	31
ATACGCC	25	4.44323E-5	44.0	41
GTACGTC	25	4.44323E-5	44.0	18
AGATGGG	134720	0.0	42.946705	1
GATGGGG	131155	0.0	42.923107	2
ATGGGGG	119370	0.0	42.92	3
>>END_MODULE