FastQCFastQC Report
Fri 17 Jun 2016
SRR1527854_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527854_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1673329
Sequences flagged as poor quality0
Sequence length52
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC183701.097811607878666No Hit
AGATGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT87190.521057126243554No Hit
AGATGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC52750.31523986018290484No Hit
AGATGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC52090.3112956268611851No Hit
AGATGGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT49970.2986262713429338No Hit
AGATGGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG47240.2823114880576384No Hit
AGATGGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGAT37790.2258372382239237No Hit
AGATGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT31880.19051842166125132No Hit
AGATGGGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCAT30200.18047855502414648No Hit
AGATGGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA29500.17629527725868613No Hit
AGATGGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC24550.14671352734578794No Hit
AGATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT24050.14372547179903056No Hit
AGATGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC23580.1409166995850786No Hit
AGATGGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA22770.13607604959933162No Hit
AGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGTTC19420.1160560774360571No Hit
AGATGGGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCT18900.11294849966742941No Hit
AGATGGGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC17520.10470146635837901No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTAGT700.046.00000417
ATAACGC351.01994374E-746.00000434
TCGGTAA351.01994374E-746.00000436
TAATACG253.417828E-546.029
GCGACGT301.8619157E-646.017
CGTATAA301.8619157E-646.019
CCGATAC253.417828E-546.030
TAACGTA253.417828E-546.011
CGTTAAC206.3122844E-446.012
CATACCG206.3122844E-446.011
CAATGCG253.417828E-546.028
ACGTATT206.3122844E-446.013
ACCGTAT206.3122844E-446.044
GACGATA453.110472E-1046.040
TCGCGTA206.3122844E-446.019
AATAACG206.3122844E-446.022
CGCTTAT206.3122844E-446.014
GATGGGG1639200.044.887322
ATGGGGG1506400.044.8808443
AGATGGG1689200.044.864431
Produced by FastQC (version 0.11.5)