##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527854_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1673329
Sequences flagged as poor quality	0
Sequence length	52
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.03542041045126	31.0	31.0	34.0	31.0	34.0
2	32.53955856857797	34.0	31.0	34.0	31.0	34.0
3	31.96829135214892	31.0	31.0	34.0	30.0	34.0
4	36.20515511295149	37.0	35.0	37.0	35.0	37.0
5	36.201304704573936	37.0	37.0	37.0	35.0	37.0
6	36.3417923193825	37.0	37.0	37.0	35.0	37.0
7	36.61622490257445	37.0	37.0	37.0	35.0	37.0
8	36.62549265565827	37.0	37.0	37.0	35.0	37.0
9	38.48164825924848	39.0	39.0	39.0	37.0	39.0
10	35.98333382138241	35.0	35.0	39.0	33.0	39.0
11	35.37714639500062	35.0	35.0	39.0	31.0	39.0
12	35.143898779020745	35.0	35.0	39.0	31.0	39.0
13	35.26980528037224	35.0	35.0	39.0	31.0	39.0
14	35.952787527139016	35.0	35.0	40.0	32.0	40.0
15	36.09152354378607	35.0	35.0	40.0	32.0	40.0
16	36.19062778449426	35.0	35.0	40.0	33.0	40.0
17	36.03397777723329	35.0	35.0	40.0	32.0	40.0
18	36.0054382610951	35.0	35.0	40.0	32.0	40.0
19	35.99852509578212	35.0	35.0	40.0	32.0	40.0
20	35.931480898257306	35.0	35.0	40.0	32.0	40.0
21	35.902664688175484	35.0	35.0	40.0	31.0	40.0
22	35.89819276424421	35.0	35.0	40.0	31.0	40.0
23	35.87142396982303	35.0	35.0	40.0	32.0	40.0
24	35.78885323806615	35.0	35.0	40.0	32.0	40.0
25	35.74070072293016	35.0	35.0	40.0	31.0	40.0
26	35.64164548633293	35.0	35.0	40.0	31.0	40.0
27	35.548072734052894	35.0	35.0	40.0	31.0	40.0
28	35.43738679004547	35.0	34.0	40.0	31.0	40.0
29	35.36731031375181	35.0	34.0	40.0	31.0	40.0
30	35.21309616937255	35.0	34.0	40.0	30.0	40.0
31	35.08908648568213	35.0	34.0	40.0	30.0	40.0
32	35.05047662474026	35.0	34.0	40.0	30.0	40.0
33	34.91830895179609	35.0	34.0	40.0	30.0	40.0
34	34.64899371253352	35.0	34.0	40.0	27.0	40.0
35	34.55659466847225	35.0	34.0	40.0	27.0	40.0
36	34.631497452085036	35.0	34.0	40.0	29.0	40.0
37	34.53919462341237	35.0	34.0	40.0	29.0	40.0
38	34.44100413009038	35.0	34.0	39.0	28.0	40.0
39	34.31419105268599	35.0	34.0	39.0	27.0	40.0
40	34.13033240922736	35.0	33.0	39.0	27.0	40.0
41	34.04307700398427	35.0	33.0	39.0	26.0	40.0
42	33.98811530786833	35.0	33.0	39.0	26.0	40.0
43	33.90634537499798	35.0	33.0	39.0	26.0	40.0
44	33.81927702203213	35.0	33.0	39.0	26.0	40.0
45	33.772193633170765	35.0	33.0	38.0	25.0	40.0
46	33.62031555061796	35.0	33.0	38.0	24.0	40.0
47	33.466483877348686	35.0	33.0	38.0	23.0	40.0
48	33.29001409764607	35.0	33.0	38.0	23.0	40.0
49	33.63672714690297	35.0	33.0	38.0	26.0	40.0
50	33.753644979558715	35.0	33.0	38.0	27.0	40.0
51	33.79710983315295	35.0	33.0	37.0	27.0	40.0
52	33.324483111211244	35.0	33.0	37.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	0.0
13	1.0
14	20.0
15	48.0
16	143.0
17	376.0
18	744.0
19	1403.0
20	2325.0
21	3860.0
22	5820.0
23	8331.0
24	11471.0
25	15307.0
26	20615.0
27	25325.0
28	29166.0
29	34421.0
30	42110.0
31	55243.0
32	79574.0
33	140330.0
34	352669.0
35	168313.0
36	92054.0
37	149935.0
38	308011.0
39	125712.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	100.0	0.0	0.0	0.0
3	0.0	100.0	0.0	0.0
4	0.0	0.0	100.0	0.0
5	100.0	0.0	0.0	0.0
6	100.0	0.0	0.0	0.0
7	98.46234661563864	1.2255211019470766	0.18370565501464445	0.1284266273996327
8	97.05628719755649	2.0861408605241407	0.5589456705764377	0.2986262713429338
9	90.51621050014671	5.140292195975806	2.82956908055738	1.5139282233201001
10	51.49650785948251	33.52927009571937	7.023603845986055	7.950618198812069
11	36.09098987706542	23.956914629460197	21.217166498638342	18.734928994836043
12	30.89237083681691	19.19807760458344	25.39614146411136	24.513410094488293
13	25.89054513487784	18.351860273741742	27.817721440314486	27.93987315106593
14	25.255702853413766	19.79443373060528	29.689618718136124	25.260244697844836
15	27.21562824764287	21.049656104687124	26.865189093119163	24.869526554550838
16	31.750480628734696	21.71999648604668	22.952509637973165	23.57701324724546
17	30.40041737159877	22.30200994544408	23.223944603840607	24.073628079116542
18	30.578266437741767	21.80688914134638	23.439801736538364	24.175042684373484
19	29.506630196452697	24.12484335118796	20.994078271517434	25.374448180841902
20	31.015717769787056	23.69157529690814	22.133483612606966	23.15922332069784
21	30.637609220900373	23.26517977038586	23.330797470192653	22.766413538521117
22	28.628978521259118	21.495234947819586	25.49456801382155	24.381218517099747
23	29.26160964161859	21.653900697352405	24.331258228357964	24.75323143267104
24	30.70782852624917	22.51278738371235	22.701931299822093	24.077452790216388
25	28.561149660347724	23.03450188217619	21.368362109304268	27.03598634817182
26	27.314353602907737	23.248566181545886	23.201773231683667	26.235306983862706
27	26.874272781981308	21.307525298372287	23.03438235995432	28.783819559692088
28	28.12142740608691	24.03812997922106	21.53282468659779	26.307617928094235
29	30.400477132709707	22.462109961639342	21.6921478083509	25.44526509730005
30	28.01756259528162	21.650673597361905	23.472969153107368	26.858794654249106
31	30.5167722545895	22.91019877143108	22.524560322566572	24.04846865141284
32	33.26907021870774	21.352107087129905	21.415692909164903	23.96312978499745
33	31.70488290109118	22.7043217442595	21.97392144641012	23.616873908239207
34	27.857522340197292	24.70052213282624	25.415922391830897	22.02603313514557
35	27.856924729087947	26.196940350642343	24.17301080660169	21.773124113668025
36	27.995510745346554	27.25799887529589	21.321091070554566	23.42539930880299
37	30.426234171522754	25.72118214648763	20.512104911825467	23.340478770164147
38	30.572051282204516	25.588871046877216	20.764356561082728	23.074721109835544
39	30.712131326236502	23.987452557148057	20.60443582822027	24.69598028839517
40	28.791349459669917	23.1259961430179	22.17149167916172	25.911162718150464
41	26.927280887380782	23.783248841082656	22.528624078110163	26.760846193426396
42	26.45110435544953	22.48099447269485	23.124860681910135	27.943040489945492
43	25.859170551636883	21.106787726741125	24.453768505775013	28.580273215846972
44	25.93865282918063	20.96371962716238	24.97357064868893	28.124056894968053
45	27.782641668195556	21.52475693662155	23.836675274258678	26.855926120924217
46	27.33240145841015	23.97310989052362	22.477349044927806	26.217139606138424
47	25.17723651475591	23.75671490782745	24.464704789076148	26.601343788340486
48	26.885985959724596	22.547090261389123	25.37289439195759	25.19402938692869
49	27.500330180137915	23.123725220802367	23.883049896344353	25.49289470271536
50	25.694588452121494	22.56967996132261	24.14468404001843	27.591047546537474
51	25.31193805880374	21.998363740782594	25.07731593727235	27.612382263141317
52	25.38508565858836	22.566034533555566	24.886797515611097	27.162082292244982
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	17.0
6	34.0
7	148.0
8	262.0
9	555.5
10	849.0
11	1233.5
12	1618.0
13	1902.5
14	2738.0
15	3289.0
16	2759.0
17	2229.0
18	2469.0
19	2709.0
20	3070.5
21	3432.0
22	4093.0
23	4754.0
24	5795.0
25	6836.0
26	7711.5
27	8587.0
28	9700.5
29	10814.0
30	11948.0
31	13082.0
32	14355.0
33	15628.0
34	18990.0
35	22352.0
36	24886.0
37	27420.0
38	30050.0
39	33678.0
40	34676.0
41	37692.0
42	40708.0
43	44439.5
44	48171.0
45	50455.5
46	52740.0
47	56141.5
48	59543.0
49	62168.0
50	64793.0
51	70354.5
52	75916.0
53	85167.0
54	94418.0
55	101431.5
56	108445.0
57	122542.5
58	136640.0
59	143727.5
60	150815.0
61	147066.0
62	143317.0
63	148103.5
64	127355.0
65	101820.0
66	92029.0
67	82238.0
68	68589.5
69	54941.0
70	46006.5
71	37072.0
72	32022.0
73	26972.0
74	27176.0
75	27380.0
76	20447.0
77	13514.0
78	8754.5
79	3995.0
80	3433.5
81	2872.0
82	1715.5
83	559.0
84	329.0
85	99.0
86	59.5
87	20.0
88	13.0
89	5.0
90	4.0
91	2.5
92	1.0
93	1.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1673329.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	9.57553383183265
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.39118703978956	6.836087267941976
2	8.806456734886495	1.6865304880695229
3	3.952842671367043	1.135517361947606
4	1.9731316615072376	0.7557515591769091
5	1.0827828854508985	0.5184112076082228
6	0.650915317124658	0.3739716984471145
7	0.4554004657735943	0.30524917969331794
8	0.3230136224145027	0.24744222956583103
9	0.24560671947417861	0.21166339064853856
>10	4.9189848349080005	13.589843822938485
>50	3.9197239828062203	26.869636142347947
>100	2.176376512580086	35.95354351519698
>500	0.07425137757942378	4.791160974419319
>1k	0.0268303297135733	4.480294151250549
>5k	0.001871883468388835	1.1473333856063623
>10k+	6.239611561296118E-4	1.0975636251413254
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	18370	1.097811607878666	No Hit
AGATGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	8719	0.521057126243554	No Hit
AGATGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	5275	0.31523986018290484	No Hit
AGATGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	5209	0.3112956268611851	No Hit
AGATGGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	4997	0.2986262713429338	No Hit
AGATGGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	4724	0.2823114880576384	No Hit
AGATGGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGAT	3779	0.2258372382239237	No Hit
AGATGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT	3188	0.19051842166125132	No Hit
AGATGGGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCAT	3020	0.18047855502414648	No Hit
AGATGGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	2950	0.17629527725868613	No Hit
AGATGGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	2455	0.14671352734578794	No Hit
AGATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT	2405	0.14372547179903056	No Hit
AGATGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	2358	0.1409166995850786	No Hit
AGATGGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA	2277	0.13607604959933162	No Hit
AGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGTTC	1942	0.1160560774360571	No Hit
AGATGGGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCT	1890	0.11294849966742941	No Hit
AGATGGGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC	1752	0.10470146635837901	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.033107655458071907	0.0	0.0	0.0	0.0
8	0.0830679441998555	0.0	0.0	0.0	0.0
9	0.11157399411592102	0.0	0.0	0.0	0.0
10	0.1435461884662251	0.0	0.0	0.0	0.0
11	0.15830718286720663	0.0	0.0	0.0	0.0
12	0.17097653838545798	0.0	0.0	0.0	0.0
13	0.18394469945838504	0.0	0.0	0.0	0.0
14	0.20031924385461555	0.0	0.0	0.0	0.0
15	0.21908423268825197	0.0	0.0	0.0	0.0
16	0.23766993818908297	0.0	0.0	0.0	0.0
17	0.2578094325742278	0.0	0.0	0.0	0.0
18	0.27717203251721567	0.0	0.0	0.0	0.0
19	0.30687330465198415	0.0	0.0	0.0	0.0
20	0.3300008545838864	0.0	0.0	0.0	0.0
21	0.35306864340485344	0.0	0.0	0.0	0.0
22	0.37476192667431213	0.0	0.0	0.0	0.0
23	0.39878589327024155	0.0	0.0	0.0	0.0
24	0.4249612598598363	0.0	0.0	0.0	0.0
25	0.44510075424498113	0.0	0.0	0.0	0.0
26	0.46350717641300665	0.0	0.0	0.0	0.0
27	0.4780291263702476	0.0	0.0	0.0	0.0
28	0.4930291652149697	0.0	0.0	0.0	0.0
29	0.506116848509767	0.0	0.0	0.0	0.0
30	0.5180690706967966	0.0	0.0	0.0	0.0
31	0.5293041595526045	0.0	0.0	0.0	0.0
32	0.5410770984068285	0.0	0.0	0.0	0.0
33	0.5508779206001928	0.0	0.0	0.0	0.0
34	0.5660572427777203	0.0	0.0	0.0	0.0
35	0.5797425371818692	0.0	0.0	0.0	0.0
36	0.5930692649204071	0.0	0.0	0.0	0.0
37	0.6044238759980852	0.0	0.0	0.0	0.0
38	0.6170334704054015	0.0	0.0	0.0	0.0
39	0.6308980481423557	0.0	0.0	0.0	0.0
40	0.6427905092184502	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTAGT	70	0.0	46.000004	17
ATAACGC	35	1.01994374E-7	46.000004	34
TCGGTAA	35	1.01994374E-7	46.000004	36
TAATACG	25	3.417828E-5	46.0	29
GCGACGT	30	1.8619157E-6	46.0	17
CGTATAA	30	1.8619157E-6	46.0	19
CCGATAC	25	3.417828E-5	46.0	30
TAACGTA	25	3.417828E-5	46.0	11
CGTTAAC	20	6.3122844E-4	46.0	12
CATACCG	20	6.3122844E-4	46.0	11
CAATGCG	25	3.417828E-5	46.0	28
ACGTATT	20	6.3122844E-4	46.0	13
ACCGTAT	20	6.3122844E-4	46.0	44
GACGATA	45	3.110472E-10	46.0	40
TCGCGTA	20	6.3122844E-4	46.0	19
AATAACG	20	6.3122844E-4	46.0	22
CGCTTAT	20	6.3122844E-4	46.0	14
GATGGGG	163920	0.0	44.88732	2
ATGGGGG	150640	0.0	44.880844	3
AGATGGG	168920	0.0	44.86443	1
>>END_MODULE