FastQCFastQC Report
Fri 17 Jun 2016
SRR1527853_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527853_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1690578
Sequences flagged as poor quality0
Sequence length52
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC174711.0334335357493118No Hit
AGATGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT83910.49633912188612417No Hit
AGATGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC50090.2962891981322364No Hit
AGATGGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT49670.2938048407112833No Hit
AGATGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC48890.2891910340723705No Hit
AGATGGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG45830.27109071571971244No Hit
AGATGGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGAT35670.21099292667951433No Hit
AGATGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT29960.1772174960279857No Hit
AGATGGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA28950.17124320794426523No Hit
AGATGGGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCAT27450.16237050286943283No Hit
AGATGGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC23120.13675796088674996No Hit
AGATGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC23020.1361664472150945No Hit
AGATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT22910.13551578217627344No Hit
AGATGGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA22240.13155264057618163No Hit
AGATGGGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCT19210.11362977632502021No Hit
AGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGTTC18340.10848360738161741No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTAAC206.312302E-446.012
ACGATAG253.4178433E-546.032
CCCGTAA405.6134013E-946.019
TATTACG301.8619266E-646.028
GCGATAT206.312302E-446.022
TCCCGTA453.110472E-1046.018
CGTAATC206.312302E-446.027
ACGTACT206.312302E-446.011
ATACGAG253.4178433E-546.031
TATACGC206.312302E-446.028
CGATAAT206.312302E-446.010
CGCTTAT253.4178433E-546.014
ATGGGGG1511600.044.940993
GATGGGG1655100.044.920252
AGATGGG1704650.044.9165531
TGGGGGG812400.044.658054
TGGGGGA512700.044.2683834
TGGGGGT90300.042.7397584
ATGGGGT49200.042.6808973
ACGTATT650.042.4615413
Produced by FastQC (version 0.11.5)