##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527853_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1690578
Sequences flagged as poor quality	0
Sequence length	52
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.01415787973107	31.0	31.0	34.0	31.0	34.0
2	32.52988800280141	34.0	31.0	34.0	31.0	34.0
3	31.97986901521255	31.0	31.0	34.0	30.0	34.0
4	36.202847783420815	37.0	35.0	37.0	35.0	37.0
5	36.21358257353402	37.0	37.0	37.0	35.0	37.0
6	36.24939695181175	37.0	37.0	37.0	35.0	37.0
7	36.57155659188751	37.0	37.0	37.0	35.0	37.0
8	36.59430857375406	37.0	37.0	37.0	35.0	37.0
9	38.44316381734531	39.0	39.0	39.0	37.0	39.0
10	35.904501300738566	35.0	35.0	39.0	33.0	39.0
11	35.3472930559844	35.0	35.0	39.0	31.0	39.0
12	35.09434288154702	35.0	35.0	39.0	31.0	39.0
13	35.066755866928354	35.0	35.0	39.0	30.0	39.0
14	35.874367228249746	35.0	35.0	40.0	31.0	40.0
15	36.037391353726356	35.0	35.0	40.0	32.0	40.0
16	36.16954970430231	35.0	35.0	40.0	33.0	40.0
17	36.04006558703591	35.0	35.0	40.0	32.0	40.0
18	36.02697243191382	35.0	35.0	40.0	32.0	40.0
19	35.9776183056919	35.0	35.0	40.0	32.0	40.0
20	35.91838176055763	35.0	35.0	40.0	31.0	40.0
21	35.954335144548196	35.0	35.0	40.0	32.0	40.0
22	35.83619862555883	35.0	35.0	40.0	31.0	40.0
23	35.824464768854206	35.0	35.0	40.0	32.0	40.0
24	35.7983121748893	35.0	35.0	40.0	32.0	40.0
25	35.72079194216416	35.0	35.0	40.0	31.0	40.0
26	35.57980051792937	35.0	35.0	40.0	31.0	40.0
27	35.48477562111893	35.0	34.0	40.0	31.0	40.0
28	35.38685112429003	35.0	34.0	40.0	31.0	40.0
29	35.315125359492434	35.0	34.0	40.0	31.0	40.0
30	35.15447142929815	35.0	34.0	40.0	30.0	40.0
31	35.052903208251855	35.0	34.0	40.0	30.0	40.0
32	35.0122478820853	35.0	34.0	40.0	30.0	40.0
33	34.72426057833475	35.0	34.0	40.0	29.0	40.0
34	34.55327941094703	35.0	33.0	40.0	27.0	40.0
35	34.53770781354069	35.0	33.0	40.0	27.0	40.0
36	34.468752107267456	35.0	34.0	40.0	28.0	40.0
37	34.44600249145559	35.0	34.0	40.0	28.0	40.0
38	34.08376957466618	35.0	33.0	39.0	26.0	40.0
39	34.11602718123624	35.0	33.0	39.0	26.0	40.0
40	34.00864438079758	35.0	33.0	39.0	26.0	40.0
41	33.74128848240069	35.0	33.0	39.0	24.0	40.0
42	33.822071504538684	35.0	33.0	39.0	25.0	40.0
43	33.85438826247591	35.0	33.0	39.0	26.0	40.0
44	33.77231988112941	35.0	33.0	39.0	25.0	40.0
45	33.71906590527027	35.0	33.0	38.0	25.0	40.0
46	33.39667675788991	35.0	33.0	38.0	23.0	40.0
47	33.15930113842721	35.0	33.0	38.0	23.0	40.0
48	33.14518052405745	35.0	33.0	38.0	23.0	40.0
49	33.13028266072314	35.0	33.0	37.0	24.0	40.0
50	33.037442815415794	35.0	32.0	37.0	24.0	40.0
51	33.39768942929578	35.0	33.0	37.0	25.0	40.0
52	33.12933564733482	35.0	33.0	37.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	2.0
12	1.0
13	6.0
14	18.0
15	54.0
16	126.0
17	401.0
18	842.0
19	1476.0
20	2571.0
21	4036.0
22	6218.0
23	8785.0
24	11905.0
25	16088.0
26	20899.0
27	26367.0
28	30867.0
29	35690.0
30	44755.0
31	59399.0
32	86864.0
33	155523.0
34	348715.0
35	157513.0
36	98208.0
37	160424.0
38	303608.0
39	109216.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	100.0	0.0	0.0	0.0
3	0.0	100.0	0.0	0.0
4	0.0	0.0	100.0	0.0
5	100.0	0.0	0.0	0.0
6	100.0	0.0	0.0	0.0
7	98.4485187906148	1.2354946059868284	0.1902899481715721	0.12569665522679227
8	97.0512451954302	2.0813000050870176	0.5785003708790721	0.28895442860370835
9	90.56216276326795	5.104585532285408	2.836189752853758	1.4970619515928871
10	51.557514648836076	33.48062023757555	7.069712252259286	7.892152861329084
11	36.07186417899677	23.912945749915117	21.291534611239467	18.723655459848644
12	30.863231391867156	19.154691472384002	25.465314229807795	24.516762905941043
13	26.090070969810323	18.3023794228956	27.773045668404535	27.834503938889537
14	25.161808564881362	19.847649738728414	29.732316403029024	25.258225293361203
15	27.292381658817284	21.084978037097372	26.834904985158918	24.787735318926426
16	31.6957277333551	21.765455364969853	22.979596327409915	23.559220574265133
17	30.434029071713937	22.293677073758207	23.164621803903753	24.107672050624107
18	30.556294947645124	21.845960375682164	23.396436011825543	24.201308664847172
19	29.53977870290516	24.084188957859386	20.987023373071224	25.389008966164234
20	30.978103346902657	23.675630464846932	22.096762172464093	23.249504015786318
21	30.6406447972232	23.20443067400617	23.341898451298903	22.81302607747173
22	28.834339498088816	21.433734497905448	25.39853233627789	24.333393667727844
23	29.25987443347778	21.687789619881485	24.292165164813454	24.76017078182728
24	30.617634915395797	22.51555385199618	22.772034180026	24.094777052582018
25	28.514093996254537	23.068678286361234	21.378487120972828	27.038740596411404
26	27.395245886318172	23.17012288105015	23.149715659378035	26.28491557325364
27	26.801839370913378	21.32394956044619	23.07098518968069	28.803225878959744
28	28.074658489581672	24.033377933464177	21.585990116989574	26.305973459964584
29	30.442960928155934	22.451256315887232	21.67081317750497	25.434969578451867
30	28.058510166345474	21.659574417743517	23.394779773544904	26.8871356423661
31	30.491287595130185	22.877678521783672	22.5726940726781	24.05833981040804
32	33.30156904916544	21.37127065417863	21.349325496960212	23.977834799695724
33	31.663076178679717	22.726665081410026	22.003303012342524	23.60695572756773
34	27.846925725994303	24.617438532856813	25.51908282256128	22.016552918587607
35	27.815634652763727	26.253565348655904	24.192672565241	21.738127433339365
36	28.162675723924007	27.178751882492257	21.371034048709966	23.287538344873767
37	30.535414515035686	25.76444269356398	20.504111611531677	23.19603117986866
38	30.61272535192106	25.571490933869956	20.708716190557315	23.107067523651676
39	30.716476849929432	23.958492302632592	20.631996867343595	24.69303398009438
40	28.83510846586197	23.096361126194708	22.181112022042164	25.88741838590115
41	27.16437810027103	23.79541198335717	22.545484443781948	26.49472547258985
42	26.45834738178304	22.474029592245966	23.09902293771716	27.968600088253844
43	25.890375954259433	21.097222370100642	24.395739208720332	28.616662466919596
44	25.89262370621172	20.9776183056919	25.015586385248124	28.11417160284826
45	27.85532522013181	21.535888909000352	23.846696218689704	26.76208965217813
46	27.45469301031955	23.934476847563378	22.44185124850791	26.168978893609168
47	25.401371601901836	23.720053141588263	24.438860555384018	26.439714701125887
48	26.946464463633145	22.44380324362437	25.381141834331217	25.228590458411265
49	27.598253378430336	23.01130146021065	23.876271902272478	25.514173259086537
50	25.78928626777351	22.552464305107485	24.16238706525224	27.49586236186677
51	25.373866216169855	21.9762116861807	25.046404247541375	27.603517850108066
52	25.397526763036076	22.5302825424204	24.88752367533471	27.184667019208813
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	17.0
6	34.0
7	146.5
8	259.0
9	559.0
10	859.0
11	1249.0
12	1639.0
13	1982.0
14	2700.5
15	3076.0
16	2678.5
17	2281.0
18	2505.0
19	2729.0
20	3086.5
21	3444.0
22	4106.5
23	4769.0
24	5772.0
25	6775.0
26	7639.5
27	8504.0
28	9757.0
29	11010.0
30	12144.0
31	13278.0
32	14393.0
33	15508.0
34	18880.0
35	22252.0
36	24943.5
37	27635.0
38	30364.5
39	34045.5
40	34997.0
41	38189.0
42	41381.0
43	45131.5
44	48882.0
45	51048.5
46	53215.0
47	56734.5
48	60254.0
49	62997.0
50	65740.0
51	71411.5
52	77083.0
53	85902.5
54	94722.0
55	102343.5
56	109965.0
57	123847.5
58	137730.0
59	144706.0
60	151682.0
61	148750.5
62	145819.0
63	149708.0
64	128706.5
65	103816.0
66	93400.0
67	82984.0
68	69293.5
69	55603.0
70	46483.5
71	37364.0
72	32448.5
73	27533.0
74	27587.0
75	27641.0
76	20568.0
77	13495.0
78	8793.0
79	4091.0
80	3455.0
81	2819.0
82	1690.5
83	562.0
84	325.0
85	88.0
86	55.0
87	22.0
88	17.5
89	9.0
90	5.0
91	3.5
92	2.0
93	2.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1690578.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	10.127618768135981
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.55619432603486	7.246938566327361
2	9.019598544334704	1.8269411099731223
3	3.8098022117946044	1.1575267314917104
4	2.007479149692908	0.8132393405228623
5	1.0926697214238268	0.5533071189032931
6	0.6396378917605953	0.38868052304433215
7	0.44782598459641754	0.3174787592520358
8	0.3073642785203757	0.24902945886380257
9	0.25898503412922036	0.2360611523082087
>10	4.991725840914534	14.481829524114337
>50	3.819401360958304	27.4729194629367
>100	1.95277663567793	34.106498834483325
>500	0.07026574252282915	4.757489265328807
>1k	0.02444025826881014	4.481750182496607
>5k	0.001222012913440507	0.8291974214433288
>10k+	6.110064567202535E-4	1.081112548510179
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	17471	1.0334335357493118	No Hit
AGATGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	8391	0.49633912188612417	No Hit
AGATGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	5009	0.2962891981322364	No Hit
AGATGGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	4967	0.2938048407112833	No Hit
AGATGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	4889	0.2891910340723705	No Hit
AGATGGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	4583	0.27109071571971244	No Hit
AGATGGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGAT	3567	0.21099292667951433	No Hit
AGATGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT	2996	0.1772174960279857	No Hit
AGATGGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	2895	0.17124320794426523	No Hit
AGATGGGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCAT	2745	0.16237050286943283	No Hit
AGATGGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	2312	0.13675796088674996	No Hit
AGATGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	2302	0.1361664472150945	No Hit
AGATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT	2291	0.13551578217627344	No Hit
AGATGGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA	2224	0.13155264057618163	No Hit
AGATGGGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCT	1921	0.11362977632502021	No Hit
AGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGTTC	1834	0.10848360738161741	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.031823435535065524	0.0	0.0	0.0	0.0
8	0.07849386422868392	0.0	0.0	0.0	0.0
9	0.10315998433671797	0.0	0.0	0.0	0.0
10	0.13279481928665818	0.0	0.0	0.0	0.0
11	0.1491205966243498	0.0	0.0	0.0	0.0
12	0.16112832415895628	0.0	0.0	0.0	0.0
13	0.17272199212340394	0.0	0.0	0.0	0.0
14	0.18975758586708213	0.0	0.0	0.0	0.0
15	0.206970633712257	0.0	0.0	0.0	0.0
16	0.22489349796341843	0.0	0.0	0.0	0.0
17	0.2404503075279579	0.0	0.0	0.0	0.0
18	0.25955619912243033	0.0	0.0	0.0	0.0
19	0.28883612586937724	0.0	0.0	0.0	0.0
20	0.3093024989086573	0.0	0.0	0.0	0.0
21	0.3322532293688904	0.0	0.0	0.0	0.0
22	0.3541983865873092	0.0	0.0	0.0	0.0
23	0.3776814793520323	0.0	0.0	0.0	0.0
24	0.40287996176455626	0.0	0.0	0.0	0.0
25	0.42376039437399515	0.0	0.0	0.0	0.0
26	0.4416832586251566	0.0	0.0	0.0	0.0
27	0.45806818733001375	0.0	0.0	0.0	0.0
28	0.4743939646677054	0.0	0.0	0.0	0.0
29	0.4869932058739674	0.0	0.0	0.0	0.0
30	0.49982905254889154	0.0	0.0	0.0	0.0
31	0.510535450005856	0.0	0.0	0.0	0.0
32	0.5233121453136146	0.0	0.0	0.0	0.0
33	0.5337227859347513	0.0	0.0	0.0	0.0
34	0.5476233572186554	0.0	0.0	0.0	0.0
35	0.5609915661980696	0.0	0.0	0.0	0.0
36	0.5730584450998416	0.0	0.0	0.0	0.0
37	0.5841197507597993	0.0	0.0	0.0	0.0
38	0.5950627536854259	0.0	0.0	0.0	0.0
39	0.606597270282708	0.0	0.0	0.0	0.0
40	0.6180726355128247	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTAAC	20	6.312302E-4	46.0	12
ACGATAG	25	3.4178433E-5	46.0	32
CCCGTAA	40	5.6134013E-9	46.0	19
TATTACG	30	1.8619266E-6	46.0	28
GCGATAT	20	6.312302E-4	46.0	22
TCCCGTA	45	3.110472E-10	46.0	18
CGTAATC	20	6.312302E-4	46.0	27
ACGTACT	20	6.312302E-4	46.0	11
ATACGAG	25	3.4178433E-5	46.0	31
TATACGC	20	6.312302E-4	46.0	28
CGATAAT	20	6.312302E-4	46.0	10
CGCTTAT	25	3.4178433E-5	46.0	14
ATGGGGG	151160	0.0	44.94099	3
GATGGGG	165510	0.0	44.92025	2
AGATGGG	170465	0.0	44.916553	1
TGGGGGG	81240	0.0	44.65805	4
TGGGGGA	51270	0.0	44.268383	4
TGGGGGT	9030	0.0	42.739758	4
ATGGGGT	4920	0.0	42.680897	3
ACGTATT	65	0.0	42.46154	13
>>END_MODULE