SRR1527852_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1527852_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	18205
Sequences flagged as poor quality	0
Sequence length	50
%GC	46

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GTGGTAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC	345	1.8950837681955508	No Hit
GTGGTAGGGGTACGCTTAGGTGGGCAGTCCTGTGGGGAAAACATCTTGTC	345	1.8950837681955508	No Hit
GTGGTAGGGGAGCCGGCGTGGTGGCACACGCCTTTAATCCCAGCACTCGG	273	1.4995880252677836	No Hit
GTGGTAGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	239	1.3128261466630047	No Hit
GTGGTAGAAGTCGTCATTTGGCGAGAAAGCTCAGTCTCAGGAGGAAGCGG	200	1.098599285910464	No Hit
GTGGTAGGGTCACATCGCGGAAGGGGATGAACCGCTTCCCGGTGCCGTTC	180	0.9887393573194178	No Hit
GTGGTAGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAA	166	0.9118374073056853	No Hit
GTGGTAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	165	0.906344410876133	No Hit
GTGGTAGGGCCTCGACCCCAAAAAAACTTGATTAGGGTGATGGTTCACGT	133	0.7305685251304587	No Hit
GTGGTATCGTTATGCGCCTTCGTATGTTTCTCCTGCTTATCACCTTCTTG	110	0.6042296072507553	No Hit
GTGGTAGGGAGGACTCTCGCCAAGCTCTCTCTTTTTCGGCAATTTTACAT	90	0.4943696786597089	No Hit
GTGGTAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGTGGTAGGGA	90	0.4943696786597089	No Hit
GTGGTAGGGGAGTGCAGTGCTGTGGTAGGGAAAAAAAAAAAAAAAAAAAA	89	0.48887668223015657	No Hit
GTGGTAGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAA	88	0.4833836858006042	No Hit
GTGGTAGGGAGTTGGTGGAGTGATTTGTCTGCTTAATTGCGATAACGAAC	85	0.46690469651194727	No Hit
GTGGTAGGGACAGCCATCTGTAATGGGATCTGGTGCCCTCTTCTGGCCTG	80	0.43943971436418566	No Hit
GTGGTAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	77	0.4229607250755287	No Hit
GTGGTAGGGAAGCAGATCAACGCAGAGTACAGTGCTGTGGTAGGGACACA	71	0.39000274649821476	No Hit
GTGGTAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA	66	0.36253776435045315	Illumina Single End Adapter 2 (100% over 34bp)
GTGGTAGGGGGAGAGGTGATTTAAGCCAACTGGGAGTCAACTACGAACCT	66	0.36253776435045315	No Hit
GTGGTAGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCG	66	0.36253776435045315	No Hit
GTGGTAGGCCAGTGCTGTGGTAGGGGGGAATTCCTTCGGCAACATTAACT	64	0.35155177149134853	No Hit
GTGGTAGGGTGGTGCGATGCAGGCCGCTGAGTTCCGCCAGCAGCCCGACG	62	0.3405657786322439	No Hit
GTGGTAGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAAC	60	0.32957978577313923	No Hit
GTGGTAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTTGTAGCGC	58	0.3185937929140346	No Hit
GTGGTAGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA	58	0.3185937929140346	No Hit
GTGGTAGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	56	0.30760780005492994	No Hit
GTGGTAGGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	56	0.30760780005492994	No Hit
GTGGTAGGGGGGAATTCGTGGAGAAAGAAATGGCTCCCCTGGCAGCATTT	52	0.2856358143367207	No Hit
GTGGTAGGGAAGCATTGGTATCACTGTGGTAGGGTAAAAAAAAAAAAAAA	49	0.2691568250480637	No Hit
GTGGTAGGGGCCTCAGCCTCCCGAGTAGCTGGAATTAAAGGCATGCACCA	49	0.2691568250480637	No Hit
GTGGTAGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA	49	0.2691568250480637	Illumina Single End Adapter 2 (97% over 34bp)
GTGGTAGGGGTACCTGCCGCGCCTTCGTGCGGCTGCATCTCCGGCGCATC	48	0.2636638286185114	No Hit
GTGGTAGGGGTTGTTGTTTGGGAATGCAGCTCTAAGTGGGTGGTAAATTC	47	0.2581708321889591	No Hit
GTGGTAGGGAGCGCCATTTTTGTGGACGCCATCTGCCAGCAGAACTTTGA	45	0.24718483932985444	No Hit
GTGGTAGGGGTACAGTGCTGTGGTAGGGGGAAAAAAAAAAAAAAAAAAAA	45	0.24718483932985444	No Hit
GTGGTAGGGGGTGTGGGAATTCGTGGAGAAAGAAATGGCTCGGGGGGCTG	44	0.2416918429003021	No Hit
GTGGTAGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAA	43	0.2361988464707498	Illumina Single End Adapter 2 (100% over 34bp)
GTGGTAGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	42	0.23070585004119748	No Hit
GTGGTAGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA	42	0.23070585004119748	Illumina Single End Adapter 2 (97% over 34bp)
GTGGTAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGTGGTAGGGG	41	0.22521285361164514	No Hit
GTGGTAGGGGGAATTCATCTGCGTGCGAAGAGGTGGCAGCCGTCTCGTTG	41	0.22521285361164514	No Hit
GTGGTAGGGGGGAATTCGTGGAGAAAGAAATGGCCCGGTGGGCTGCAATA	39	0.21422686075254052	No Hit
GTGGTATTTAAGTATTCATTTGCCTAATTACACAGGAGATACATGCATGC	37	0.20324086789343587	No Hit
GTGGTAGGGGGGAATTCCTTTCGATTCTGTTTTTGCTACCCGTTGTAGCG	35	0.19225487503433122	No Hit
GTGGTAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTTTAGCGCC	35	0.19225487503433122	No Hit
GTGGTAGGGAGTGCAGTGCTGTGGTAGGGAAAAAAAAAAAAAAAAAAAAA	35	0.19225487503433122	No Hit
GTGGTAGGGAAGCGTGGTATCAATGCTGTGGTAGGGGAAAAAAAAAAAAA	35	0.19225487503433122	No Hit
GTGGTAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	35	0.19225487503433122	No Hit
GTGGTAGGTAGTAATACTATCATTGAAGTTAATGGCAAAAGAGTCAGAGG	34	0.1867618786047789	No Hit
GTGGTAGGGGCTCCCCAAACACCTCCTCCAACATCCAAAAATACAACCCG	34	0.1867618786047789	No Hit
GTGGTAGGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAA	33	0.18126888217522658	Illumina Single End Adapter 2 (100% over 34bp)
GTGGTAGGAAGGAAGACAATGAGGTTGGGAGGGGGGTACGTTACTGATGG	31	0.17028288931612195	No Hit
GTGGTAGGGGTGCAGTGCTGTGGTGGGGCACAATTTCGGTCACGTTAACG	31	0.17028288931612195	No Hit
GTGGTAGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAG	30	0.16478989288656962	No Hit
GTGGTAGGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAA	30	0.16478989288656962	Illumina Single End Adapter 2 (97% over 34bp)
GTGGTAGGGAAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCG	29	0.1592968964570173	Illumina Single End Adapter 2 (100% over 23bp)
GTGGTAGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTT	28	0.15380390002746497	No Hit
GTGGTAGGGGGTGCCAGCGTCGGGCTACGGCGGGCATATCCCTCTTCCTG	28	0.15380390002746497	No Hit
GTGGTAGGGGGGAATTCGTGGAGAAAGAAATGGCTCCCCTGGCCGCATTT	28	0.15380390002746497	No Hit
GTGGTAGGCAATAGAAATCAGACTTTAAAAGCTCAACATGTTGATTGAAA	27	0.14831090359791266	No Hit
GTGGTAGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA	27	0.14831090359791266	Illumina Single End Adapter 2 (97% over 34bp)
GTGGTAGGGGGTGTGGGAATTCGTGGAGAAAGAAATGGCTCGGGGGGGTG	27	0.14831090359791266	No Hit
GTGGTAGGGGGTGTGGGAATTCGTGGAGAAAGAAATGGCTCCGGGGGCTG	27	0.14831090359791266	No Hit
GTGGTAGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	27	0.14831090359791266	No Hit
GTGGTAGGGGAGTGCAGTGCTGTGGTAGGGTTAAAAAAAAAAAAAAAAAA	26	0.14281790716836035	No Hit
GTGGTAGGGGTGCAGTGCTGTGGTAGGGACCAGAGTGAGTGCTGTGGTAG	26	0.14281790716836035	No Hit
GTGGTAGGGGGTGTGGGAATTCGTGGAGAAAGAAATGGCTCCGGGGGGTG	26	0.14281790716836035	No Hit
GTGGTAGGGGAGACCGGATGCTGAAAGTAGATTACAAGGGGGTTAATACT	25	0.137324910738808	No Hit
GTGGTAGGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG	24	0.1318319143092557	No Hit
GTGGTAGGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGGCAGCATTT	23	0.1263389178797034	No Hit
GTGGTAGGGGTGGTGAGTGCAGTGCTGTGGTAGGGAAAAAAAAAAAAAAA	23	0.1263389178797034	No Hit
GTGGTAGGGAAGCAGTGGTATCAACGCAGGATCGGAAGAGCTCGTATGCC	23	0.1263389178797034	Illumina Single End Adapter 2 (95% over 22bp)
GTGGTAGGGGAGTGCAGTGCTGTGGTAGGGTGAGTGCAGTGCTGTGGTAG	23	0.1263389178797034	No Hit
GTGGTAGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAGG	22	0.12084592145015105	No Hit
GTGGTAGGGGGAATTCGTGGAGAAAGAAATGGCTCCCCTGGCAGCATTTT	22	0.12084592145015105	No Hit
GTGGTAGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	21	0.11535292502059874	No Hit
GTGGTAGGGTAACAATGGCTCAAATTAAAATTACTTTGACTAAGTCTCCA	21	0.11535292502059874	No Hit
GTGGTAGGGGGAATTCGTGGAGAAAGAAATGGCTCCCCTGGCCGCATTTT	21	0.11535292502059874	No Hit
GTGGTAGGGAGGGCACCGGGCCCGAGCTGCTGCGCGACCTGCCGACGATC	21	0.11535292502059874	No Hit
GTGGTAGGGATCTTAATGGCCCGCTTGTCGTAGACTGGCACCTTGATAGC	20	0.10985992859104642	No Hit
GTGGTAGGGGTACCTGCCGCGCCTTCGTGCGGCTGCATCTCTGGCGCATC	20	0.10985992859104642	No Hit
GTGGTAGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG	19	0.10436693216149409	No Hit
GTGGTAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19	0.10436693216149409	No Hit
GTGGTAGGGGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTCTTC	19	0.10436693216149409	Illumina Single End Adapter 2 (100% over 28bp)
GTGGTAGGGGGGAATTCGTGGAGAAAGAAATGGCTCCCCTGGCAGCCTTT	19	0.10436693216149409	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAGCCAT	20	7.653794E-4	44.000004	11
ACAGCCA	20	7.653794E-4	44.000004	10
ATGGGAT	20	7.653794E-4	44.000004	23
ACGCTTA	65	0.0	44.000004	12
ATCTTGT	65	0.0	44.000004	43
AGCCATC	20	7.653794E-4	44.000004	12
AAACATC	65	0.0	44.000004	39
GACAGCC	20	7.653794E-4	44.000004	9
CATCTTG	65	0.0	44.000004	42
GGATCTG	20	7.653794E-4	44.000004	26
GAAAACA	65	0.0	44.000004	37
CGCTTAG	65	0.0	44.000004	13
AGGGTGC	20	7.653794E-4	44.000004	6
GTATCAC	20	7.653794E-4	44.000004	18
GGACAGC	20	7.653794E-4	44.000004	8
TAATGGG	20	7.653794E-4	44.000004	21
ACATCTT	65	0.0	44.000004	41
GCTACGG	20	7.653794E-4	44.000004	24
CGGGCTA	20	7.653794E-4	44.000004	21
AGGGACG	20	7.653794E-4	44.000004	6

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content