FastQCFastQC Report
Fri 17 Jun 2016
SRR1527851_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527851_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26997
Sequences flagged as poor quality0
Sequence length52
%GC46

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTAGGGGTACGCTTAGGTGGGCAGTCCTGTGGGGAAAACATCTTGTCTC4891.8113123680408936No Hit
GTGGTAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGG4701.7409341778716152No Hit
GTGGTAGGGGAGCCGGCGTGGTGGCACACGCCTTTAATCCCAGCACTCGGGA4141.5335037226358486No Hit
GTGGTAGGGTCACATCGCGGAAGGGGATGAACCGCTTCCCGGTGCCGTTCAC2931.085305774715709No Hit
GTGGTAGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAAAA2791.0334481609067674No Hit
GTGGTAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGTGGTAGGGAAG2520.9334370485609512No Hit
GTGGTAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2410.8926917805682113No Hit
GTGGTAGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2050.7593436307737897No Hit
GTGGTAGGGCCTCGACCCCAAAAAAACTTGATTAGGGTGATGGTTCACGTAG2020.7482312849575878No Hit
GTGGTAGGGGGTGCCAGCGTCGGGCTACGGCGGACATATCCCTCTTCCTGCA1540.5704337518983591No Hit
GTGGTAGGGAGTTGGTGGAGTGATTTGTCTGCTTAATTGCGATAACGAACGA1500.55561729081009No Hit
GTGGTAGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAAC1440.5333925991776864No Hit
GTGGTAGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGA1240.4593102937363411No Hit
GTGGTAGGGGGAGAGGTGATTTAAGCCAACTGGGAGTCAACTACGAACCTTA1230.4556061784642738No Hit
GTGGTAGGGAGGACTCTCGCCAAGCTCTCTCTTTTTCGGCAATTTTACATGG1220.45190206319220655No Hit
GTGGTAGAAGTCGTCATTTGGCGAGAAAGCTCAGTCTCAGGAGGAAGCGGAG1200.44449383264807196No Hit
GTGGTAGGGTGGTGCGATGCAGGCCGCTGAGTTCCGCCAGCAGCCCGACGCT1130.41856502574360116No Hit
GTGGTAGGGGAGTGCAGTGCTGTGGTAGGGAAAAAAAAAAAAAAAAAAAAAA1120.4148609104715339No Hit
GTGGTAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA980.3630032966625921No Hit
GTGGTAGGGACAGCCATCTGTAATGGGATCTGGTGCCCTCTTCTGGCCTGCA960.35559506611845765No Hit
GTGGTAGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACAT930.3444827203022558No Hit
GTGGTAGGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGGCAGCATTTTA910.33707448975812127No Hit
GTGGTAGGGGGTGTGGGAATTCGTGGAGAAAGAAATGGCTCGGGGGGCTGCA900.333370374486054No Hit
GTGGTATCGTTATGCGCCTTCGTATGTTTCTCCTGCTTATCACCTTCTTGAA870.3222580286698522No Hit
GTGGTAGGGGTACCTGCCGCGCCTTCGTGCGGCTGCATCTCCGGCGCATCCA860.31855391339778494No Hit
GTGGTAGGCCAGTGCTGTGGTAGGGGGGAATTCCTTCGGCAACATTAACTGG820.3037374523095159No Hit
GTGGTAGGGAAGCAGATCAACGCAGAGTACAGTGCTGTGGTAGGGACACAGA770.28521687594917955No Hit
GTGGTAGGTAGTAATACTATCATTGAAGTTAATGGCAAAAGAGTCAGAGGAA690.2555839537726414No Hit
GTGGTAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTTGTAGCGCCG680.2518798385005741No Hit
GTGGTAGGGAAGCATTGGTATCACTGTGGTAGGGTAAAAAAAAAAAAAAAAA660.24447160795643957No Hit
GTGGTAGGGGCTCCCCAAACACCTCCTCCAACATCCAAAAATACAACCCGGT650.24076749268437236No Hit
GTGGTAGGGGCCTCAGCCTCCCGAGTAGCTGGAATTAAAGGCATGCACCACC630.2333592621402378No Hit
GTGGTAGGGGTTGTTGTTTGGGAATGCAGCTCTAAGTGGGTGGTAAATTCCA620.22965514686817054No Hit
GTGGTAGGGGGGAATTCGTGGAGAAAGAAATGGCCCGGTGGGCTGCAATAAA600.22224691632403598No Hit
GTGGTAGGGGGGAATTCCTTTCGATTCTGTTTTTGCTACCCGTTGTAGCGCC590.21854280105196872No Hit
GTGGTAGGGGGAATTCATCTGCGTGCGAAGAGGTGGCAGCCGTCTCGTTGCA540.20002222469163242No Hit
GTGGTAGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA530.19631810941956512Illumina Single End Adapter 2 (100% over 34bp)
GTGGTAGGGGTACAGTGCTGTGGTAGGGGGAAAAAAAAAAAAAAAAAAAAAA530.19631810941956512No Hit
GTGGTAGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA500.18520576360336333No Hit
GTGGTAGGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA500.18520576360336333No Hit
GTGGTAGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA500.18520576360336333No Hit
GTGGTAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGTGGTAGGGGAG490.18150164833129606No Hit
GTGGTAGGGAGCGCCATTTTTGTGGACGCCATCTGCCAGCAGAACTTTGACT490.18150164833129606No Hit
GTGGTAGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGGCAGCATTTTAT480.17779753305922882No Hit
GTGGTAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA480.17779753305922882No Hit
GTGGTAGGGAGTGCAGTGCTGTGGTAGGGAAAAAAAAAAAAAAAAAAAAAAA470.17409341778716153No Hit
GTGGTAGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA460.17038930251509427Illumina Single End Adapter 2 (97% over 34bp)
GTGGTAGGGGTGCAGTGCTGTGGTGGGGCACAATTTCGGTCACGTTAACGCG450.166685187243027No Hit
GTGGTAGGGAAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTC440.16298107197095973Illumina Single End Adapter 2 (100% over 25bp)
GTGGTAGGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA440.16298107197095973Illumina Single End Adapter 2 (100% over 34bp)
GTGGTAGGGTAACAATGGCTCAAATTAAAATTACTTTGACTAAGTCTCCAAT420.1555728414268252No Hit
GTGGTAGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG420.1555728414268252No Hit
GTGGTAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA410.15186872615475794Illumina Single End Adapter 2 (100% over 34bp)
GTGGTAGGGATCTTAATGGCCCGCTTGTCGTAGACTGGCACCTTGATAGCTT410.15186872615475794No Hit
GTGGTAGGGGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTCTTCTG400.14816461088269067Illumina Single End Adapter 2 (100% over 30bp)
GTGGTAGGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGGCAGCATTTGA400.14816461088269067No Hit
GTGGTATTTAAGTATTCATTTGCCTAATTACACAGGAGATACATGCATGCCC390.1444604956106234No Hit
GTGGTAGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA370.13705226506648885No Hit
GTGGTAGGGGGTGTGGGAATTCGTGGAGAAAGAAATGGCTCGGTGGGCTGCA360.1333481497944216No Hit
GTGGTAGGCAATAGAAATCAGACTTTAAAAGCTCAACATGTTGATTGAAATA360.1333481497944216No Hit
GTGGTAGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAGG360.1333481497944216No Hit
GTGGTAGGAAGGAAGACAATGAGGTTGGGAGGGGGGTACGTTACTGATGGTG360.1333481497944216No Hit
GTGGTAGGGGTGCAGTGCTGTGGTAGGGACCAGAGTGAGTGCTGTGGTAGGG360.1333481497944216No Hit
GTGGTAGGGGAGACCGGATGCTGAAAGTAGATTACAAGGGGGTTAATACTGG340.12593991925028705No Hit
GTGGTAGGGGGTGTGGGAATTCGTGGAGAAAGAAATGGCTCGGGGGGGTGCA330.12223580397821979No Hit
GTGGTAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTTTAGCGCCGG330.12223580397821979No Hit
GTGGTAGGGAGAGTGCAGTGCTGTGGTAGGGAAAAAAAAAAAAAAAAAAAAA330.12223580397821979No Hit
GTGGTAGGGAAGCAGTGGTATCAACAGATCGGAAGAGCTCGTATGCCGTCTT330.12223580397821979Illumina Single End Adapter 2 (100% over 27bp)
GTGGTAGGGAGGGCACCGGGCCCGAGCTGCTGCGCGACCTGCCGACGATCAC320.11853168870615255No Hit
GTGGTAGGGGGTGTGGGAATTCGTGGAGAAAGAAATGGCTCCGGGGGCTGCA320.11853168870615255No Hit
GTGGTAGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCG310.11482757343408527No Hit
GTGGTAGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA300.11112345816201799Illumina Single End Adapter 2 (97% over 34bp)
GTGGTAGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA300.11112345816201799Illumina Single End Adapter 2 (100% over 34bp)
GTGGTAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGTGGGAGGGAAG290.10741934288995073No Hit
GTGGTAGGGAAGCAGTGGTATCAACGCAGGATCGGAAGAGCTCGTATGCCGT280.10371522761788347Illumina Single End Adapter 2 (95% over 24bp)
GTGGTAGGGAAGCAGTGGTATCAACGCAGAGATCGGAAGAGCTCGTATGCCG270.10001111234581621Illumina Single End Adapter 2 (100% over 23bp)
GTGGTAGGGGTACCTGCCGCGCCTTCGTGCGGCTGCATCTCTGGCGCATCCA270.10001111234581621No Hit
GTGGTAGGGGTGGTGAGTGCAGTGCTGTGGTAGGGAAAAAAAAAAAAAAAAA270.10001111234581621No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCCCG253.3285596E-546.041
CGTCATT206.201845E-446.012
AGGGGTT206.201845E-446.06
AGCCCGA253.3285596E-546.042
TAGAAGT206.201845E-446.05
TTGTCTG253.3285596E-546.025
TTGTCTC800.046.046
TGATGCT206.201845E-446.039
ATCCCAG550.046.037
CGCTGAG253.3285596E-546.025
TCCTTTT206.201845E-446.016
GTTGATG206.201845E-446.037
TTTTGCG206.201845E-446.019
TGGTTGA206.201845E-446.035
GTAGAAG253.3285596E-546.04
ACGCCTT550.046.028
ACACGCC550.046.026
ACGCTTA850.046.012
GCCTTTA550.046.030
CAGTCTC206.201845E-446.032
Produced by FastQC (version 0.11.5)