FastQCFastQC Report
Fri 17 Jun 2016
SRR1527850_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527850_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27297
Sequences flagged as poor quality0
Sequence length52
%GC46

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTAGGGGTACGCTTAGGTGGGCAGTCCTGTGGGGAAAACATCTTGTCTC4721.7291277429754184No Hit
GTGGTAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGG4621.6924936806242445No Hit
GTGGTAGGGGAGCCGGCGTGGTGGCACACGCCTTTAATCCCAGCACTCGGGA4321.582591493570722No Hit
GTGGTAGGGTCACATCGCGGAAGGGGATGAACCGCTTCCCGGTGCCGTTCAC2951.0807048393596366No Hit
GTGGTAGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAAAA2520.9231783712495879No Hit
GTGGTAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2180.7986225592555959No Hit
GTGGTAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGTGGTAGGGAAG1840.6740667472616038No Hit
GTGGTAGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1800.6594131223211342No Hit
GTGGTAGGGCCTCGACCCCAAAAAAACTTGATTAGGGTGATGGTTCACGTAG1680.6154522474997253No Hit
GTGGTAGGGGGTGCCAGCGTCGGGCTACGGCGGACATATCCCTCTTCCTGCA1430.5238670916217899No Hit
GTGGTAGGGAGTTGGTGGAGTGATTTGTCTGCTTAATTGCGATAACGAACGA1410.5165402791515551No Hit
GTGGTAGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAAC1360.49822324797596806No Hit
GTGGTAGGGGAGTGCAGTGCTGTGGTAGGGAAAAAAAAAAAAAAAAAAAAAA1270.46525259185991136No Hit
GTGGTAGGGGGAGAGGTGATTTAAGCCAACTGGGAGTCAACTACGAACCTTA1260.46158918562479395No Hit
GTGGTAGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGA1190.43594534197897206No Hit
GTGGTAGGGACAGCCATCTGTAATGGGATCTGGTGCCCTCTTCTGGCCTGCA1130.4139649045682676No Hit
GTGGTAGAAGTCGTCATTTGGCGAGAAAGCTCAGTCTCAGGAGGAAGCGGAG1090.39931127962779794No Hit
GTGGTAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGTGGTAGGGGAG1060.38832106092244567No Hit
GTGGTAGGGTGGTGCGATGCAGGCCGCTGAGTTCCGCCAGCAGCCCGACGCT1060.38832106092244567No Hit
GTGGTAGGGAGGACTCTCGCCAAGCTCTCTCTTTTTCGGCAATTTTACATGG1000.36634062351174124No Hit
GTGGTAGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACAT950.34802359233615415No Hit
GTGGTAGGGGGTGTGGGAATTCGTGGAGAAAGAAATGGCTCGGGGGGCTGCA940.34436018610103675No Hit
GTGGTAGGGAAGCAGATCAACGCAGAGTACAGTGCTGTGGTAGGGACACAGA900.3297065611605671No Hit
GTGGTAGGCCAGTGCTGTGGTAGGGGGGAATTCCTTCGGCAACATTAACTGG860.31505293622009745No Hit
GTGGTAGGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGGCAGCATTTTA830.30406271751474523No Hit
GTGGTATCGTTATGCGCCTTCGTATGTTTCTCCTGCTTATCACCTTCTTGAA830.30406271751474523No Hit
GTGGTAGGGGTACCTGCCGCGCCTTCGTGCGGCTGCATCTCCGGCGCATCCA800.29307249880939296No Hit
GTGGTAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTTGTAGCGCCG780.28574568633915814No Hit
GTGGTAGGGGGGAATTCGTGGAGAAAGAAATGGCCCGGTGGGCTGCAATAAA680.24911162398798403No Hit
GTGGTAGGGAGCGCCATTTTTGTGGACGCCATCTGCCAGCAGAACTTTGACT650.2381214052826318No Hit
GTGGTAGGGGCTCCCCAAACACCTCCTCCAACATCCAAAAATACAACCCGGT630.23079459281239698No Hit
GTGGTAGGGGTTGTTGTTTGGGAATGCAGCTCTAAGTGGGTGGTAAATTCCA630.23079459281239698No Hit
GTGGTAGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA620.22713118657727957No Hit
GTGGTAGGTAGTAATACTATCATTGAAGTTAATGGCAAAAGAGTCAGAGGAA620.22713118657727957No Hit
GTGGTAGGGGGGAATTCCTTTCGATTCTGTTTTTGCTACCCGTTGTAGCGCC610.22346778034216214No Hit
GTGGTAGGGAGTGCAGTGCTGTGGTAGGGAAAAAAAAAAAAAAAAAAAAAAA610.22346778034216214No Hit
GTGGTAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA590.2161409678719273No Hit
GTGGTAGGGAAGCATTGGTATCACTGTGGTAGGGTAAAAAAAAAAAAAAAAA580.21247756163680992No Hit
GTGGTAGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGGCAGCATTTTAT570.2088141554016925No Hit
GTGGTAGGGGGAATTCATCTGCGTGCGAAGAGGTGGCAGCCGTCTCGTTGCA570.2088141554016925No Hit
GTGGTAGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG560.20515074916657508No Hit
GTGGTAGGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA540.19782393669634024No Hit
GTGGTAGGGGTACAGTGCTGTGGTAGGGGGAAAAAAAAAAAAAAAAAAAAAA520.19049712422610543No Hit
GTGGTATTTAAGTATTCATTTGCCTAATTACACAGGAGATACATGCATGCCC510.18683371799098802No Hit
GTGGTAGGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA510.18683371799098802Illumina Single End Adapter 2 (100% over 34bp)
GTGGTAGGGGCCTCAGCCTCCCGAGTAGCTGGAATTAAAGGCATGCACCACC500.18317031175587062No Hit
GTGGTAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA500.18317031175587062No Hit
GTGGTAGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA490.17950690552075318Illumina Single End Adapter 2 (100% over 34bp)
GTGGTAGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA490.17950690552075318No Hit
GTGGTAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA440.16118987434516613Illumina Single End Adapter 2 (100% over 34bp)
GTGGTAGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCG440.16118987434516613No Hit
GTGGTAGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA430.15752646811004872Illumina Single End Adapter 2 (97% over 34bp)
GTGGTAGGGGGTGTGGGAATTCGTGGAGAAAGAAATGGCTCGGGGGGGTGCA420.15386306187493132No Hit
GTGGTAGGGTAACAATGGCTCAAATTAAAATTACTTTGACTAAGTCTCCAAT410.15019965563981388No Hit
GTGGTAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTTTAGCGCCGG400.14653624940469648No Hit
GTGGTAGGGGTGGTGAGTGCAGTGCTGTGGTAGGGAAAAAAAAAAAAAAAAA400.14653624940469648No Hit
GTGGTAGGGAAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTC380.13920943693446167Illumina Single End Adapter 2 (100% over 25bp)
GTGGTAGGGGAGACCGGATGCTGAAAGTAGATTACAAGGGGGTTAATACTGG370.13554603069934426No Hit
GTGGTAGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA360.13188262446422683No Hit
GTGGTAGGGAGGGCACCGGGCCCGAGCTGCTGCGCGACCTGCCGACGATCAC340.12455581199399202No Hit
GTGGTAGGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA330.1208924057588746Illumina Single End Adapter 2 (97% over 34bp)
GTGGTAGGGGGTGTGGGAATTCGTGGAGAAAGAAATGGCTCGGTGGGCTGCA320.11722899952375718No Hit
GTGGTAGGGGTACCTGCCGCGCCTTCGTGCGGCTGCATCTCTGGCGCATCCA320.11722899952375718No Hit
GTGGTAGGGATCTTAATGGCCCGCTTGTCGTAGACTGGCACCTTGATAGCTT310.11356559328863979No Hit
GTGGTAGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA300.10990218705352237Illumina Single End Adapter 2 (97% over 34bp)
GTGGTAGGGGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTCTTCTG290.10623878081840496Illumina Single End Adapter 2 (100% over 30bp)
GTGGTAGGAAGGAAGACAATGAGGTTGGGAGGGGGGTACGTTACTGATGGTG290.10623878081840496No Hit
GTGGTAGGGGTGCAGTGCTGTGGTGGGGCACAATTTCGGTCACGTTAACGCG290.10623878081840496No Hit
GTGGTAGGGGTGCAGTGCTGTGGTAGGGACCAGAGTGAGTGCTGTGGTAGGG280.10257537458328754No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTCAC405.2714313E-946.046
ATCCCAG452.8740033E-1046.037
GAGGTGA206.203073E-446.013
GGTGATT206.203073E-446.015
CCAACTG206.203073E-446.026
ACGCCTT452.8740033E-1046.028
ACACGCC452.8740033E-1046.026
ACGCTTA650.046.012
GCCTTTT206.203073E-446.045
GCCTTTA452.8740033E-1046.030
ACTCGGG452.8740033E-1046.045
AGCTCTC253.3295488E-546.023
TGGCACA452.8740033E-1046.022
TCCCAGC452.8740033E-1046.038
AATCCCA452.8740033E-1046.036
AACCGCT405.2714313E-946.030
GGCACAC452.8740033E-1046.023
CACACGC452.8740033E-1046.025
CCCAGCA452.8740033E-1046.039
CACATCG405.2714313E-946.011
Produced by FastQC (version 0.11.5)