FastQCFastQC Report
Fri 17 Jun 2016
SRR1527849_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527849_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences757746
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG33670.44434414698328994No Hit
ATACAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC19450.2566823183494205No Hit
ATACAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC18760.24757636463933827No Hit
ATACAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGT13860.1829108962633917No Hit
ATACAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGC13240.17472873495868008No Hit
ATACAGGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG10590.13975659389821918No Hit
ATACAGGGGGCACTCTAGCCACTTGCCCCAATTTAAGTTTAGAAATTACA10540.1390967421800973No Hit
ATACAGGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCA8820.11639784307670381No Hit
ATACAGGGGGGGCTGGAGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC8130.10729188936662154No Hit
ATACAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGT8090.10676400799212402No Hit
ATACAGGGTTTCAACCAGTTGATTACCCAGGTGATTGGTACCATCCTGTT7950.10491642318138268No Hit
ATACAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7620.10056140184177811No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGAG207.854781E-444.014
AACGTAC207.854781E-444.029
ACTATCG302.5265708E-644.027
ACCGGTC207.854781E-444.017
ACCGGTA254.441399E-544.011
ACACGTA254.441399E-544.033
TACCCGA502.7284841E-1144.027
CAAACGT207.854781E-444.027
AGATACG207.854781E-444.010
CGACGGC302.5265708E-644.044
GCGAATA551.8189894E-1244.033
CGACGAA207.854781E-444.011
ACGCACG302.5265708E-644.011
CGCATCG207.854781E-444.030
ACCGATT207.854781E-444.019
CCGCTTA502.7284841E-1144.020
CGCGAAA207.854781E-444.026
CGACAAT207.854781E-444.031
TAACGAG302.5265708E-644.021
CGTTCGT408.305506E-944.027
Produced by FastQC (version 0.11.5)