##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527849_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	757746
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.901662298448294	33.0	31.0	34.0	3.0	34.0
2	30.36487424545956	34.0	31.0	34.0	16.0	34.0
3	32.385316451686975	34.0	31.0	34.0	28.0	34.0
4	36.28562737381655	37.0	35.0	37.0	35.0	37.0
5	36.23698310515661	37.0	35.0	37.0	35.0	37.0
6	36.445009277515155	37.0	37.0	37.0	35.0	37.0
7	36.68308245771011	37.0	37.0	37.0	35.0	37.0
8	36.72637796834295	37.0	37.0	37.0	35.0	37.0
9	38.68760772079298	39.0	39.0	39.0	39.0	39.0
10	38.25701752302223	39.0	39.0	39.0	37.0	39.0
11	37.2980418240413	39.0	37.0	39.0	35.0	39.0
12	37.290696354715166	39.0	37.0	39.0	35.0	39.0
13	37.29109754455979	39.0	37.0	39.0	34.0	39.0
14	38.14828319779979	40.0	38.0	40.0	35.0	40.0
15	38.22682138869753	40.0	38.0	40.0	35.0	40.0
16	38.298190950529595	40.0	38.0	40.0	35.0	40.0
17	38.21596814763786	40.0	38.0	40.0	35.0	40.0
18	38.13633460288804	40.0	38.0	40.0	34.0	40.0
19	38.18074922203483	40.0	38.0	40.0	34.0	40.0
20	38.07350616169534	40.0	38.0	40.0	34.0	40.0
21	38.04843575551702	40.0	38.0	40.0	34.0	40.0
22	38.0944195020495	40.0	38.0	40.0	34.0	40.0
23	38.057983809878245	40.0	38.0	40.0	34.0	40.0
24	37.98097251585624	40.0	37.0	40.0	34.0	40.0
25	37.98077324063736	40.0	37.0	40.0	34.0	40.0
26	37.87936063007921	40.0	37.0	40.0	34.0	40.0
27	37.829735821766135	40.0	37.0	40.0	34.0	40.0
28	37.70828615393549	40.0	37.0	40.0	34.0	40.0
29	37.69138603173095	40.0	37.0	40.0	34.0	40.0
30	37.87174198214177	40.0	37.0	40.0	34.0	40.0
31	37.92711937773343	40.0	37.0	40.0	34.0	40.0
32	37.89952041977127	40.0	37.0	40.0	34.0	40.0
33	37.88095483183019	40.0	37.0	40.0	34.0	40.0
34	37.58033694668134	40.0	36.0	40.0	34.0	40.0
35	37.59744690173224	40.0	36.0	40.0	34.0	40.0
36	37.657857646229736	40.0	36.0	40.0	34.0	40.0
37	37.588582453750995	40.0	36.0	40.0	34.0	40.0
38	37.53057752861777	40.0	35.0	40.0	34.0	40.0
39	37.438833065433535	40.0	35.0	40.0	34.0	40.0
40	37.36277987610624	40.0	35.0	40.0	34.0	40.0
41	37.26836697257392	40.0	35.0	40.0	33.0	40.0
42	37.20701395982295	40.0	35.0	40.0	33.0	40.0
43	37.149725633655606	40.0	35.0	40.0	33.0	40.0
44	36.83662599340676	40.0	35.0	40.0	33.0	40.0
45	36.86455487722799	39.0	35.0	40.0	33.0	40.0
46	36.69240616248717	39.0	35.0	40.0	33.0	40.0
47	36.664642241595466	39.0	35.0	40.0	33.0	40.0
48	36.64931784529381	39.0	35.0	40.0	33.0	40.0
49	36.56700002375466	39.0	35.0	40.0	33.0	40.0
50	36.145621355968885	38.0	35.0	40.0	31.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	2.0
15	6.0
16	27.0
17	51.0
18	83.0
19	145.0
20	231.0
21	359.0
22	611.0
23	906.0
24	1347.0
25	2119.0
26	2918.0
27	3936.0
28	5258.0
29	6433.0
30	8137.0
31	10633.0
32	14941.0
33	25653.0
34	49781.0
35	53130.0
36	54616.0
37	127654.0
38	229362.0
39	159406.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	0.0	0.0	100.0	0.0
3	0.0	100.0	0.0	0.0
4	0.0	0.0	0.0	100.0
5	0.0	100.0	0.0	0.0
6	100.0	0.0	0.0	0.0
7	99.16409984348317	0.5982215676493178	0.2053458546795364	0.03233273418797328
8	98.55703626281102	1.1229356538998556	0.26011354728365443	0.059914536005468845
9	94.81026623697123	2.2292430445030393	2.1393712404948357	0.8211194780308968
10	51.73290258213174	28.55653477550525	13.368199898118895	6.342362744244114
11	25.680505076899117	26.232009142905405	34.32337485120344	13.764110928992038
12	26.947156435005926	24.928142147896526	26.227126240191307	21.897575176906244
13	21.934790813808323	24.216030173699366	29.962810757166647	23.886368255325664
14	22.00249160008763	27.18417517215531	28.57316303880192	22.240170188955137
15	23.70121386322066	28.12037278982667	26.65484212387792	21.52357122307475
16	28.43960905105405	27.299253311795773	24.403428061645986	19.85770957550419
17	26.515349470666948	26.15375072913615	25.068822534200113	22.262077265996787
18	25.9239903608861	24.489604696032707	26.32425641309885	23.262148529982344
19	24.87944509109913	27.078730867599432	26.065066658220566	21.976757383080876
20	26.075888226397765	26.39776389449763	25.37868890103016	22.147658978074446
21	25.485848820053157	25.193534508925154	28.115489887112567	21.20512678390912
22	23.24644405909104	25.503532846098825	28.091471284572933	23.158551810237206
23	22.192132983875865	26.410960928860067	29.384120800373744	22.01278528689033
24	23.11236218996867	28.32096771213573	27.62403232745537	20.942637770440225
25	24.45568831772124	29.480327180875914	24.169048731369084	21.894935770033758
26	22.82440290018027	29.18827681043516	25.76588988922409	22.22143040016048
27	22.51229303750861	26.542667331797198	25.635371219379582	25.30966841131461
28	22.402889622643997	28.12908283250588	24.4484299488219	25.01959759602822
29	24.822169961966146	27.146299683535112	25.654902830235986	22.376627524262748
30	24.798547270457384	25.390830172643604	26.951907367376403	22.85871518952261
31	24.566279465678473	25.674962322466893	26.71766000744313	23.041098204411504
32	26.20271172662079	26.049098246642018	25.891525656354503	21.856664370382685
33	27.955673800983444	26.034581508843335	25.571497573065383	20.438247117107842
34	23.77300573015232	27.03610444660876	27.578634529248585	21.612255293990334
35	23.98178281376609	28.714767217510882	26.63016366962016	20.673286299102866
36	24.106890699522005	29.69504292995278	24.288217951661903	21.909848418863312
37	24.856086340277614	28.767291414273384	24.96562172548585	21.411000519963153
38	25.22388768795876	29.692535493423915	24.25522536575581	20.82835145286151
39	23.670464773156176	27.696879957136034	24.63094493405442	24.001710335653375
40	22.889200338899844	28.517999435166928	25.38475953683688	23.208040689096347
41	22.25138766816321	29.084548120346398	25.317982542962948	23.34608166852745
42	22.99490858414297	28.177647918959654	25.55434142839421	23.273102068503164
43	22.082333657980378	27.824231338733558	25.967408603938523	24.126026399347538
44	22.932618581952262	27.413803570061734	25.997761782972127	23.65581606501387
45	24.014115547954063	28.04330210915003	24.75473311637409	23.187849226521816
46	23.610550237150708	29.258353062899705	24.570898427705327	22.56019827224426
47	22.15980024968789	29.785574585679107	25.336590361413986	22.71803480321902
48	22.469666616517937	29.849316261649683	25.190631161365417	22.490385960466963
49	23.834899821312156	29.428726776518783	24.71989294565725	22.016480456511815
50	22.76528018623655	30.034734594441936	24.81504356341043	22.384941655911085
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	5.0
4	10.0
5	13.0
6	16.0
7	54.0
8	92.0
9	522.0
10	952.0
11	1871.0
12	2790.0
13	3263.5
14	3737.0
15	3832.0
16	3927.0
17	4170.5
18	4414.0
19	5014.0
20	5614.0
21	5488.5
22	5363.0
23	5880.5
24	6398.0
25	7895.5
26	9393.0
27	10468.5
28	11544.0
29	12402.0
30	13260.0
31	15073.0
32	16886.0
33	20019.5
34	23153.0
35	23879.5
36	24606.0
37	26695.0
38	28784.0
39	32073.5
40	35363.0
41	36311.0
42	37259.0
43	38949.0
44	40639.0
45	40230.5
46	39822.0
47	43103.5
48	46385.0
49	48782.5
50	51180.0
51	50345.0
52	49510.0
53	52507.0
54	55504.0
55	54001.5
56	52499.0
57	51996.5
58	51494.0
59	48768.0
60	46042.0
61	42853.5
62	39665.0
63	30534.0
64	21403.0
65	17079.5
66	12756.0
67	9511.0
68	6266.0
69	5287.0
70	4308.0
71	3446.0
72	2584.0
73	2397.0
74	2210.0
75	1643.5
76	1077.0
77	644.5
78	212.0
79	248.0
80	284.0
81	286.0
82	288.0
83	165.5
84	43.0
85	25.5
86	8.0
87	6.0
88	4.0
89	3.0
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	757746.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	9.0942083494997
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.62878205221227	6.423128594542235
2	9.175603314420048	1.6688969654739187
3	3.3318338146304654	0.9090117268847345
4	1.5353136654525403	0.5584984942183793
5	0.872139426216424	0.3965708825912641
6	0.5398267330324622	0.2945578069696178
7	0.33086154605215423	0.21062466842451164
8	0.2887782792297311	0.2100967870500141
9	0.20461174558488482	0.16747036605933915
>10	6.048381245374468	16.180487920754448
>50	4.30410239294162	27.44587236356246
>100	2.7005848122941183	42.16333705489702
>500	0.029022942636153883	1.7863505712996175
>1k	0.010158029922653859	1.5850957972724369
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ATACAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG	3367	0.44434414698328994	No Hit
ATACAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC	1945	0.2566823183494205	No Hit
ATACAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	1876	0.24757636463933827	No Hit
ATACAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGT	1386	0.1829108962633917	No Hit
ATACAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGC	1324	0.17472873495868008	No Hit
ATACAGGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG	1059	0.13975659389821918	No Hit
ATACAGGGGGCACTCTAGCCACTTGCCCCAATTTAAGTTTAGAAATTACA	1054	0.1390967421800973	No Hit
ATACAGGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCA	882	0.11639784307670381	No Hit
ATACAGGGGGGGCTGGAGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	813	0.10729188936662154	No Hit
ATACAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGT	809	0.10676400799212402	No Hit
ATACAGGGTTTCAACCAGTTGATTACCCAGGTGATTGGTACCATCCTGTT	795	0.10491642318138268	No Hit
ATACAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	762	0.10056140184177811	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.04882902714102087	0.0	0.0	0.0	0.0
6	0.04882902714102087	0.0	0.0	0.0	0.0
7	0.06294985390882961	0.0	0.0	0.0	0.0
8	0.08366919785785738	0.0	0.0	0.0	0.0
9	0.08960786332095451	0.0	0.0	0.0	0.0
10	0.10742385971024591	0.0	0.0	0.0	0.0
11	0.11943316098006455	0.0	0.0	0.0	0.0
12	0.1293309367518931	0.0	0.0	0.0	0.0
13	0.13619339462036092	0.0	0.0	0.0	0.0
14	0.15044619173179402	0.0	0.0	0.0	0.0
15	0.16205958197073952	0.0	0.0	0.0	0.0
16	0.1752566163331776	0.0	0.0	0.0	0.0
17	0.1883216803519913	0.0	0.0	0.0	0.0
18	0.1992752188728149	0.0	0.0	0.0	0.0
19	0.21748712629297945	0.0	0.0	0.0	0.0
20	0.23121204202991505	0.0	0.0	0.0	0.0
21	0.24216558055073864	0.0	0.0	0.0	0.0
22	0.2549667038823036	0.0	0.0	0.0	0.0
23	0.27027526374273175	0.0	0.0	0.0	0.0
24	0.28545185325953554	0.0	0.0	0.0	0.0
25	0.29812100624747606	0.0	0.0	0.0	0.0
26	0.31118607026628975	0.0	0.0	0.0	0.0
27	0.32755039287571297	0.0	0.0	0.0	0.0
28	0.3444425968596337	0.0	0.0	0.0	0.0
29	0.35711174984757427	0.0	0.0	0.0	0.0
30	0.3711006062717586	0.0	0.0	0.0	0.0
31	0.3821861151362066	0.0	0.0	0.0	0.0
32	0.3943273867496496	0.0	0.0	0.0	0.0
33	0.4033013701161075	0.0	0.0	0.0	0.0
34	0.4157065824167993	0.0	0.0	0.0	0.0
35	0.4312790829644762	0.0	0.0	0.0	0.0
36	0.4439482359524168	0.0	0.0	0.0	0.0
37	0.4559575372222354	0.0	0.0	0.0	0.0
38	0.466911075743059	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCGAG	20	7.854781E-4	44.0	14
AACGTAC	20	7.854781E-4	44.0	29
ACTATCG	30	2.5265708E-6	44.0	27
ACCGGTC	20	7.854781E-4	44.0	17
ACCGGTA	25	4.441399E-5	44.0	11
ACACGTA	25	4.441399E-5	44.0	33
TACCCGA	50	2.7284841E-11	44.0	27
CAAACGT	20	7.854781E-4	44.0	27
AGATACG	20	7.854781E-4	44.0	10
CGACGGC	30	2.5265708E-6	44.0	44
GCGAATA	55	1.8189894E-12	44.0	33
CGACGAA	20	7.854781E-4	44.0	11
ACGCACG	30	2.5265708E-6	44.0	11
CGCATCG	20	7.854781E-4	44.0	30
ACCGATT	20	7.854781E-4	44.0	19
CCGCTTA	50	2.7284841E-11	44.0	20
CGCGAAA	20	7.854781E-4	44.0	26
CGACAAT	20	7.854781E-4	44.0	31
TAACGAG	30	2.5265708E-6	44.0	21
CGTTCGT	40	8.305506E-9	44.0	27
>>END_MODULE