FastQCFastQC Report
Fri 17 Jun 2016
SRR1527848_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527848_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences955680
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT42700.4468022769127742No Hit
ATACAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA24970.26127992633517494No Hit
ATACAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC23260.24338690775154861No Hit
ATACAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC17700.18520843797086892No Hit
ATACAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC17480.18290641218818013No Hit
ATACAGGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG13800.1443997990959317No Hit
ATACAGGGGGCACTCTAGCCACTTGCCCCAATTTAAGTTTAGAAATTACAAG13140.1374937217478654No Hit
ATACAGGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACA10380.10861376192867905No Hit
ATACAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGTTC10150.10620709861041353No Hit
ATACAGGGGGGGCTGGAGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC9900.10359116022099447No Hit
ATACAGGGGGTGAACTCTTGGAAATGCTGTCAATCAAGCTTACTGCTCTAGC9680.10128913443830571No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGCC351.0193253E-746.00000431
ATACTAG351.0193253E-746.00000435
AAACGCG351.0193253E-746.00000436
AATTGCG351.0193253E-746.00000444
TCGCTAG351.0193253E-746.00000414
AACGCGT351.0193253E-746.00000437
CATACGG551.8189894E-1246.00000438
CCGACGC351.0193253E-746.00000424
CTAGGTC351.0193253E-746.00000430
TCGCATT253.4167213E-546.030
TCGTTAC301.8610717E-646.027
CCGGGTC253.4167213E-546.012
TCGCAAT206.310917E-446.029
CAAACGA301.8610717E-646.014
ACCGGGT405.6097633E-946.018
CAACGAC206.310917E-446.044
CGACGCT206.310917E-446.044
GCGCGTA206.310917E-446.030
CCGATAC206.310917E-446.044
GCAACCG301.8610717E-646.023
Produced by FastQC (version 0.11.5)