##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527848_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	955680
Sequences flagged as poor quality	0
Sequence length	52
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.24697492884648	33.0	31.0	34.0	31.0	34.0
2	32.664066423907585	34.0	31.0	34.0	31.0	34.0
3	32.978907165578434	34.0	31.0	34.0	31.0	34.0
4	36.4363479407333	37.0	37.0	37.0	35.0	37.0
5	36.18586451531893	37.0	37.0	37.0	35.0	37.0
6	36.25604386405492	37.0	37.0	37.0	35.0	37.0
7	36.6269870668006	37.0	37.0	37.0	35.0	37.0
8	36.66390214297673	37.0	37.0	37.0	35.0	37.0
9	38.61104449188013	39.0	39.0	39.0	38.0	39.0
10	37.99205068642223	39.0	38.0	39.0	35.0	39.0
11	36.93475849656789	39.0	35.0	39.0	34.0	39.0
12	36.96193286455718	39.0	37.0	39.0	33.0	39.0
13	36.98365875606898	39.0	37.0	39.0	33.0	39.0
14	37.852872300351585	40.0	37.0	40.0	33.0	40.0
15	37.95026473296501	40.0	37.0	40.0	34.0	40.0
16	38.01932341369496	40.0	37.0	40.0	34.0	40.0
17	37.926051607232544	40.0	37.0	40.0	34.0	40.0
18	37.89355223505776	40.0	37.0	40.0	34.0	40.0
19	37.952076008705845	40.0	37.0	40.0	34.0	40.0
20	37.913215720743345	40.0	37.0	40.0	34.0	40.0
21	37.87130524861878	40.0	37.0	40.0	34.0	40.0
22	37.893731165243594	40.0	37.0	40.0	34.0	40.0
23	37.87275343211117	40.0	37.0	40.0	34.0	40.0
24	37.804317344717894	40.0	37.0	40.0	34.0	40.0
25	37.79284488531726	40.0	36.0	40.0	34.0	40.0
26	37.685849866063954	40.0	36.0	40.0	34.0	40.0
27	37.6268688263854	40.0	36.0	40.0	34.0	40.0
28	37.5615655868073	40.0	36.0	40.0	34.0	40.0
29	37.49672484513645	40.0	36.0	40.0	33.0	40.0
30	37.386323874100114	40.0	36.0	40.0	33.0	40.0
31	37.30326887661142	40.0	35.0	40.0	33.0	40.0
32	37.21590490540767	40.0	35.0	40.0	33.0	40.0
33	37.169090071990624	39.0	35.0	40.0	33.0	40.0
34	37.02767662816005	39.0	35.0	40.0	32.0	40.0
35	36.949521806462414	39.0	35.0	40.0	32.0	40.0
36	37.01408211953792	39.0	35.0	40.0	33.0	40.0
37	36.92302653607902	39.0	35.0	40.0	33.0	40.0
38	36.85650531558681	39.0	35.0	40.0	32.0	40.0
39	36.768051021262345	39.0	35.0	40.0	32.0	40.0
40	36.675387158881634	39.0	35.0	40.0	32.0	40.0
41	36.575908253808805	39.0	35.0	40.0	32.0	40.0
42	36.527623263016906	39.0	35.0	40.0	31.0	40.0
43	36.42887682069312	38.0	35.0	40.0	31.0	40.0
44	36.3450652938222	38.0	35.0	40.0	31.0	40.0
45	36.247769127741506	38.0	35.0	40.0	31.0	40.0
46	36.120723463920974	38.0	35.0	40.0	31.0	40.0
47	35.97001402142977	38.0	35.0	40.0	31.0	40.0
48	35.86654214799933	38.0	35.0	40.0	30.0	40.0
49	36.122094215637034	38.0	35.0	40.0	31.0	40.0
50	36.14297672861209	38.0	35.0	40.0	31.0	40.0
51	36.12613950276243	38.0	35.0	40.0	31.0	40.0
52	35.69112987610916	37.0	35.0	40.0	30.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	8.0
16	24.0
17	59.0
18	117.0
19	232.0
20	370.0
21	669.0
22	1073.0
23	1625.0
24	2499.0
25	3551.0
26	4826.0
27	6527.0
28	8295.0
29	10156.0
30	13208.0
31	17456.0
32	23231.0
33	33747.0
34	73017.0
35	68069.0
36	73398.0
37	124273.0
38	324433.0
39	164815.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	0.0	0.0	100.0	0.0
3	0.0	100.0	0.0	0.0
4	0.0	0.0	0.0	100.0
5	0.0	100.0	0.0	0.0
6	100.0	0.0	0.0	0.0
7	99.30175372509626	0.5204671019588146	0.15423572744014735	0.023543445504771474
8	98.668905909928	1.078499079189687	0.1993345052737318	0.05326050560857191
9	94.83687008203583	2.258078017746526	2.1057257659467603	0.7993261342708856
10	51.626485853005185	28.626946258161727	13.366189519504438	6.380378369328646
11	25.728381885149844	26.180416038841454	34.342353088900055	13.748848987108655
12	27.034153691612257	24.92277749874435	26.085614431608906	21.957454378034488
13	21.938410346559518	24.174409844299348	29.981688431274065	23.90549137786707
14	22.242696300016743	27.0190858864892	28.569500251130087	22.16871756236397
15	23.880692281935374	27.917085216808974	26.57573664825046	21.62648585300519
16	28.487150510631174	27.220722417545623	24.40042692114515	19.89170015067805
17	26.598652268541773	25.813347564038168	25.171500920810313	22.416499246609746
18	26.054641721078188	24.31263602879625	26.357148836430603	23.27557341369496
19	25.093650594341206	26.821320944249123	25.995730788548464	22.089297672861207
20	26.145467101958815	26.13646827389921	25.458103130755067	22.259961493386907
21	25.63033651431442	25.04499414029801	28.094655114682737	21.23001423070484
22	23.470617780010045	25.294450025113008	27.878892516323457	23.356039678553493
23	22.187238406161057	26.222584965678884	29.32309978235393	22.267076845806127
24	23.17281935375858	28.141951280763433	27.635191695965176	21.050037669512808
25	24.5100870584296	29.03220743345053	24.315461242256823	22.142244265863052
26	23.045580110497237	28.83140800267872	25.77850326469111	22.34450862213293
27	22.717855349070817	26.21473715051063	25.599363803783692	25.468043696634858
28	22.531181985601876	27.907249288464758	24.49010128913444	25.071467436798926
29	24.875376695128075	26.89111418047882	25.627720575924997	22.605788548468105
30	24.982734806629832	25.28084714548803	26.889335342374014	22.84708270550812
31	24.68880796919471	25.310982755734134	26.778942742340533	23.221266532730624
32	26.296354428260504	25.89444165411016	25.85384229030638	21.955361627322954
33	28.177214130252803	25.62259333668174	25.631592164741335	20.568600368324123
34	23.79488950276243	26.73635526536079	27.738364306043866	21.730390925832914
35	24.19376778838105	28.30560438640549	26.680374183827222	20.820253641386238
36	24.16394609074167	29.402519671856687	24.40994893688264	22.023585300519002
37	24.938368491545287	28.495730788548467	25.108613761928677	21.457286957977566
38	25.381194542106144	29.2817679558011	24.352921479993302	20.984116022099446
39	23.79070400133936	27.28266783860707	24.803699983257992	24.12292817679558
40	23.011782186505943	28.117152184831745	25.542440984429938	23.32862464423238
41	22.410430269546293	28.796040515653775	25.348756906077348	23.444772308722587
42	23.053323288129917	27.79832161392935	25.61757073497405	23.530784362966685
43	22.224384731290808	27.36899380545789	26.080591829901223	24.326029633350075
44	22.84059517830236	26.98298593671522	26.217143813828898	23.959275071153524
45	24.210300519002175	27.499790724928847	24.907500418550143	23.382408337518832
46	23.546898543445504	28.74518667336347	24.81154779842625	22.896366984764775
47	22.27921479993303	29.338795412690438	25.496609743847316	22.885380043529217
48	22.569897873765278	29.379604051565376	25.367068474803283	22.683429599866063
49	24.05282102795915	29.017976728612087	24.952389921312573	21.97681232211619
50	22.984890339862716	29.579147831910262	24.96703917629332	22.468922651933703
51	22.50575506445672	29.218776159383896	25.599886991461577	22.67558178469781
52	22.17426335174954	29.722815168257156	25.77243428762766	22.330487192365645
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	5.0
6	10.0
7	61.5
8	113.0
9	579.5
10	1046.0
11	2125.5
12	3205.0
13	3707.0
14	4294.0
15	4379.0
16	4581.0
17	4783.0
18	5445.5
19	6108.0
20	6074.5
21	6041.0
22	5889.5
23	5738.0
24	8050.0
25	10362.0
26	11052.5
27	11743.0
28	12389.5
29	13036.0
30	16920.0
31	20804.0
32	20870.0
33	20936.0
34	24541.0
35	28146.0
36	29462.0
37	30778.0
38	35728.5
39	39214.5
40	37750.0
41	43719.5
42	49689.0
43	48883.5
44	48078.0
45	48851.5
46	49625.0
47	54894.5
48	60164.0
49	58310.5
50	56457.0
51	59493.0
52	62529.0
53	64544.0
54	66559.0
55	67251.0
56	67943.0
57	64879.0
58	61815.0
59	60154.5
60	58494.0
61	54397.0
62	50300.0
63	40097.5
64	24413.0
65	18931.0
66	13958.0
67	8985.0
68	7528.0
69	6071.0
70	5143.0
71	4215.0
72	3352.5
73	2490.0
74	2320.5
75	2151.0
76	1328.5
77	506.0
78	373.5
79	241.0
80	247.5
81	254.0
82	308.5
83	363.0
84	200.0
85	37.0
86	20.5
87	4.0
88	5.0
89	9.0
90	12.0
91	6.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	955680.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	7.469445839611585
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.75070043707274	5.1352963335007535
2	9.203743135716687	1.3749372174786538
3	4.034517538944301	0.9040683073832245
4	2.0999103440546905	0.6274066633182656
5	1.133307183682618	0.42325883140800263
6	0.666816093242183	0.2988448016072326
7	0.4580858455676342	0.23951531893520844
8	0.3165975568754903	0.18918466432278588
9	0.2577608427658859	0.17327975891511802
>10	4.741958982405021	10.31286623137452
>50	4.5052112518211365	24.522225012556504
>100	3.7473383391236132	50.53574418215302
>500	0.07144458141880533	3.4470743345052735
>1k	0.012607867309200941	1.8162983425414363
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ATACAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	4270	0.4468022769127742	No Hit
ATACAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	2497	0.26127992633517494	No Hit
ATACAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	2326	0.24338690775154861	No Hit
ATACAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC	1770	0.18520843797086892	No Hit
ATACAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC	1748	0.18290641218818013	No Hit
ATACAGGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG	1380	0.1443997990959317	No Hit
ATACAGGGGGCACTCTAGCCACTTGCCCCAATTTAAGTTTAGAAATTACAAG	1314	0.1374937217478654	No Hit
ATACAGGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACA	1038	0.10861376192867905	No Hit
ATACAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGTTC	1015	0.10620709861041353	No Hit
ATACAGGGGGGGCTGGAGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	990	0.10359116022099447	No Hit
ATACAGGGGGTGAACTCTTGGAAATGCTGTCAATCAAGCTTACTGCTCTAGC	968	0.10128913443830571	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.047610078687426755	0.0	0.0	0.0	0.0
6	0.047610078687426755	0.0	0.0	0.0	0.0
7	0.059434120207600874	0.0	0.0	0.0	0.0
8	0.07502511300853842	0.0	0.0	0.0	0.0
9	0.07973380210949271	0.0	0.0	0.0	0.0
10	0.09511551983927674	0.0	0.0	0.0	0.0
11	0.10809057425079524	0.0	0.0	0.0	0.0
12	0.11688012723924326	0.0	0.0	0.0	0.0
13	0.1256696802276913	0.0	0.0	0.0	0.0
14	0.1368658965344048	0.0	0.0	0.0	0.0
15	0.14994558848150008	0.0	0.0	0.0	0.0
16	0.1607232546459066	0.0	0.0	0.0	0.0
17	0.1736983090574251	0.0	0.0	0.0	0.0
18	0.1880336514314415	0.0	0.0	0.0	0.0
19	0.20352000669680229	0.0	0.0	0.0	0.0
20	0.21482086053909258	0.0	0.0	0.0	0.0
21	0.22842374016407166	0.0	0.0	0.0	0.0
22	0.2377364808304035	0.0	0.0	0.0	0.0
23	0.24966515988615437	0.0	0.0	0.0	0.0
24	0.2632680395111334	0.0	0.0	0.0	0.0
25	0.27435961828227023	0.0	0.0	0.0	0.0
26	0.2877532228360958	0.0	0.0	0.0	0.0
27	0.30030972710530723	0.0	0.0	0.0	0.0
28	0.31537753222836096	0.0	0.0	0.0	0.0
29	0.3265737485350745	0.0	0.0	0.0	0.0
30	0.3370375020927507	0.0	0.0	0.0	0.0
31	0.3486522685417713	0.0	0.0	0.0	0.0
32	0.3594299347061778	0.0	0.0	0.0	0.0
33	0.36874267537250965	0.0	0.0	0.0	0.0
34	0.3791017913946091	0.0	0.0	0.0	0.0
35	0.39050728277247615	0.0	0.0	0.0	0.0
36	0.40264523689938053	0.0	0.0	0.0	0.0
37	0.41310899045705674	0.0	0.0	0.0	0.0
38	0.42388665662146324	0.0	0.0	0.0	0.0
39	0.43382722250125566	0.0	0.0	0.0	0.0
40	0.4434538757743178	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGCC	35	1.0193253E-7	46.000004	31
ATACTAG	35	1.0193253E-7	46.000004	35
AAACGCG	35	1.0193253E-7	46.000004	36
AATTGCG	35	1.0193253E-7	46.000004	44
TCGCTAG	35	1.0193253E-7	46.000004	14
AACGCGT	35	1.0193253E-7	46.000004	37
CATACGG	55	1.8189894E-12	46.000004	38
CCGACGC	35	1.0193253E-7	46.000004	24
CTAGGTC	35	1.0193253E-7	46.000004	30
TCGCATT	25	3.4167213E-5	46.0	30
TCGTTAC	30	1.8610717E-6	46.0	27
CCGGGTC	25	3.4167213E-5	46.0	12
TCGCAAT	20	6.310917E-4	46.0	29
CAAACGA	30	1.8610717E-6	46.0	14
ACCGGGT	40	5.6097633E-9	46.0	18
CAACGAC	20	6.310917E-4	46.0	44
CGACGCT	20	6.310917E-4	46.0	44
GCGCGTA	20	6.310917E-4	46.0	30
CCGATAC	20	6.310917E-4	46.0	44
GCAACCG	30	1.8610717E-6	46.0	23
>>END_MODULE