FastQCFastQC Report
Fri 17 Jun 2016
SRR1527847_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527847_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences965476
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT41040.42507529964494195No Hit
ATACAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA23810.24661410537392953No Hit
ATACAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC21190.21947723195605068No Hit
ATACAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC16420.17007155019907277No Hit
ATACAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC16410.1699679743463328No Hit
ATACAGGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG13510.13993097705173407No Hit
ATACAGGGGGCACTCTAGCCACTTGCCCCAATTTAAGTTTAGAAATTACAAG11390.117972896270855No Hit
ATACAGGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACA10030.1038865802982156No Hit
ATACAGGGGGGGCTGGAGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC9890.10243651835985566No Hit
ATACAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGTTC9750.10098645642149572No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGATAG351.0193435E-746.00000430
CTTATCG351.0193435E-746.00000426
TACGATC351.0193435E-746.00000439
TAACACG351.0193435E-746.00000413
TCTCGCG351.0193435E-746.00000412
CGCACGT351.0193435E-746.00000422
CCGCGCA351.0193435E-746.00000433
ATACGCA351.0193435E-746.00000431
TATCGAT351.0193435E-746.00000428
CGATAGA351.0193435E-746.00000431
ACGCGAG253.416746E-546.014
AACCGGC253.416746E-546.030
ACCGGTC253.416746E-546.017
ACACGTT206.3109514E-446.023
ACACGTA301.8610917E-646.033
CGAACAG405.6097633E-946.035
CGTATAG206.3109514E-446.09
CGAAACC206.3109514E-446.016
CCGATGT453.092282E-1046.025
TTAGCGT206.3109514E-446.040
Produced by FastQC (version 0.11.5)