SRR1527846_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1527846_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1792157
Sequences flagged as poor quality	0
Sequence length	50
%GC	45

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
TAAGGGGGGGAGTGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAAAAA	15624	0.8717986203217686	No Hit
TAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	14005	0.7814605528421895	No Hit
TAAGGGGGGGAGTGCAGTGCTTAAGGGGGAAAAAAAAAAAAAAAAAAAAA	13587	0.7581367034249789	No Hit
TAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	12409	0.6924058550673853	No Hit
TAAGGGGGGCAGAGTGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAAA	10869	0.6064758835302934	No Hit
TAAGGGGGGCAGAGTGCAGTGCTTAAGGGGGAAAAAAAAAAAAAAAAAAA	10359	0.5780185552939837	No Hit
TAAGGGGGGAGTGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAAAAAA	7759	0.43294197997162076	No Hit
TAAGGGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA	7334	0.40922753977469606	Illumina Single End Adapter 2 (97% over 34bp)
TAAGGGGGGAGAGTGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAAAA	6543	0.365090781667008	No Hit
TAAGGGGGGAGTGCAGTGCTTAAGGGGGAAAAAAAAAAAAAAAAAAAAAA	6409	0.35761375816962465	No Hit
TAAGGGGGGAGAGTGCAGTGCTTAAGGGGGAAAAAAAAAAAAAAAAAAAA	6175	0.34455686639061195	No Hit
TAAGGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA	5852	0.3265338918409492	Illumina Single End Adapter 2 (97% over 34bp)
TAAGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA	5227	0.2916597150807658	Illumina Single End Adapter 2 (100% over 34bp)
TAAGGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA	4795	0.267554684104127	Illumina Single End Adapter 2 (100% over 34bp)
TAAGGGGGGGTGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAAAAAAA	4791	0.26733148937286183	No Hit
TAAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4699	0.2621980105537629	No Hit
TAAGGGGGGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAA	4531	0.25282383184062557	No Hit
TAAGGGGGGGCAGAGTGCAGTGCTTAAGGGGGAAAAAAAAAAAAAAAAAA	3860	0.21538291567089266	No Hit
TAAGGGGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA	3786	0.21125381314248695	Illumina Single End Adapter 2 (97% over 34bp)
TAAGGGGGGCAGTGCTTAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA	3775	0.21064002763150771	No Hit
TAAGGGGGGGCAGAGTGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAA	3775	0.21064002763150771	No Hit
TAAGGGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC	3578	0.19964768711669795	No Hit
TAAGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA	3519	0.1963555648305366	Illumina Single End Adapter 2 (97% over 34bp)
TAAGGGGGGGTGCAGTGCTTAAGGGGGAAAAAAAAAAAAAAAAAAAAAAA	3430	0.19138948205988648	No Hit
TAAGGGGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAA	3074	0.17152515097728604	Illumina Single End Adapter 2 (100% over 34bp)
TAAGGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC	3055	0.17046497600377647	No Hit
TAAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	2694	0.15032165150709453	No Hit
TAAGGGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTAAGGGGGGA	2539	0.14167285567056903	No Hit
TAAGGGGGGGAGTGCAGTGCTTAAGGGGAAAAAAAAAAAAAAAAAAAAAA	2475	0.13810173997032626	No Hit
TAAGGGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA	2206	0.12309189429274334	Illumina Single End Adapter 2 (100% over 34bp)
TAAGGGGGGGAGTGCAGTGCTTAAGGGGGGTAAAAAAAAAAAAAAAAAAA	1958	0.10925382095430256	No Hit
TAAGGGGGGCAGAGTGCAGTGCTTAAGGGGAAAAAAAAAAAAAAAAAAAA	1873	0.1045109329149176	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGAATT	20	7.85766E-4	44.000004	13
TATACTA	20	7.85766E-4	44.000004	30
CGTTTCG	20	7.85766E-4	44.000004	44
TGCGATG	50	2.7284841E-11	44.0	15
TCGCGAC	25	4.4438406E-5	44.0	23
TAATCGC	25	4.4438406E-5	44.0	28
CCGATAA	40	4.12625E-7	38.500004	23
ACATTCG	35	7.2924868E-6	37.714287	28
TTCGTAC	35	7.2924868E-6	37.714287	18
CAATTAG	35	7.2924868E-6	37.714287	38
ATATACG	35	7.2924868E-6	37.714287	37
CATTTCG	35	7.2924868E-6	37.714287	36
AGGGGGG	162290	0.0	37.161007	3
ACCGACC	30	1.3013858E-4	36.666664	38
GTTACGC	30	1.3013858E-4	36.666664	23
CTTATAC	30	1.3013858E-4	36.666664	20
TCGTACT	30	1.3013858E-4	36.666664	27
TCGTACA	30	1.3013858E-4	36.666664	19
TTACGCG	30	1.3013858E-4	36.666664	24
GTCCGAC	85	0.0	36.235294	10

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content