##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527846_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1792157
Sequences flagged as poor quality	0
Sequence length	50
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.072147696881466	33.0	31.0	34.0	30.0	34.0
2	31.872796300770524	33.0	31.0	34.0	30.0	34.0
3	32.6263541642836	34.0	31.0	34.0	31.0	34.0
4	36.159639473550584	37.0	35.0	37.0	35.0	37.0
5	36.29716537111425	37.0	37.0	37.0	35.0	37.0
6	36.385423821685265	37.0	37.0	37.0	35.0	37.0
7	36.531774838923155	37.0	37.0	37.0	35.0	37.0
8	36.49626957906032	37.0	37.0	37.0	35.0	37.0
9	34.75814172530643	35.0	35.0	35.0	33.0	39.0
10	33.78308987438042	35.0	33.0	35.0	27.0	39.0
11	33.44197857665372	35.0	33.0	35.0	25.0	39.0
12	33.862485820159726	35.0	33.0	35.0	27.0	39.0
13	33.91769917479328	35.0	33.0	35.0	30.0	39.0
14	34.404939968987094	35.0	34.0	35.0	30.0	40.0
15	34.47492323496211	35.0	34.0	35.0	31.0	40.0
16	34.56610107261808	35.0	34.0	35.0	31.0	40.0
17	34.59657384927771	35.0	34.0	35.0	31.0	40.0
18	34.51324744428083	35.0	34.0	35.0	31.0	40.0
19	34.52128357058003	35.0	34.0	35.0	31.0	40.0
20	34.51145519058877	35.0	34.0	35.0	31.0	40.0
21	34.47629811450671	35.0	34.0	36.0	31.0	40.0
22	34.27977738557504	35.0	34.0	35.0	31.0	39.0
23	33.96388597650764	35.0	34.0	35.0	31.0	38.0
24	33.770785148845775	35.0	34.0	35.0	30.0	37.0
25	33.66161167799473	35.0	34.0	35.0	30.0	37.0
26	33.488691001960206	35.0	34.0	35.0	29.0	37.0
27	33.336825958886415	35.0	34.0	35.0	29.0	37.0
28	33.104749751277375	35.0	33.0	35.0	27.0	36.0
29	32.79240044259515	35.0	33.0	35.0	24.0	36.0
30	32.619456889100675	35.0	33.0	35.0	23.0	36.0
31	32.443841136686125	35.0	33.0	35.0	23.0	36.0
32	32.30316205555652	35.0	33.0	35.0	22.0	36.0
33	32.03594327952295	35.0	33.0	35.0	21.0	36.0
34	31.554441379856787	35.0	33.0	35.0	18.0	36.0
35	31.22097003778129	35.0	33.0	35.0	15.0	36.0
36	30.792916022424375	35.0	32.0	35.0	12.0	36.0
37	30.335097315692767	35.0	31.0	35.0	10.0	36.0
38	29.946100704346772	35.0	31.0	35.0	9.0	36.0
39	29.643901734055667	35.0	30.0	35.0	9.0	36.0
40	29.37003566093819	35.0	29.0	35.0	8.0	35.0
41	29.147182975598678	35.0	27.0	35.0	8.0	35.0
42	29.011562603053193	35.0	26.0	35.0	8.0	35.0
43	28.92863404266479	35.0	25.0	35.0	8.0	35.0
44	28.670226994621565	35.0	23.0	35.0	8.0	35.0
45	28.63628856177221	35.0	23.0	35.0	8.0	35.0
46	28.473018825917595	35.0	23.0	35.0	8.0	35.0
47	28.334553836522137	35.0	23.0	35.0	8.0	35.0
48	28.11803262772179	35.0	23.0	35.0	8.0	35.0
49	27.919657150573304	34.0	22.0	35.0	8.0	35.0
50	27.285731105031534	34.0	18.0	35.0	8.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	6.0
11	20.0
12	22.0
13	39.0
14	120.0
15	366.0
16	1001.0
17	2240.0
18	3923.0
19	5889.0
20	8696.0
21	12919.0
22	19236.0
23	27265.0
24	37762.0
25	50923.0
26	65620.0
27	79955.0
28	78346.0
29	64953.0
30	61076.0
31	71545.0
32	94836.0
33	169091.0
34	526982.0
35	234735.0
36	50182.0
37	40966.0
38	60510.0
39	22932.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	0.0	100.0	0.0	0.0
3	0.0	100.0	0.0	0.0
4	100.0	0.0	0.0	0.0
5	100.0	0.0	0.0	0.0
6	100.0	0.0	0.0	0.0
7	97.97088089938549	1.515157433193632	0.3045492108113296	0.20941245660954927
8	94.10777069196504	4.3338836943415115	0.9371388778996483	0.6212067357937948
9	79.32809458099932	14.919675006151806	3.59505333517097	2.1571770776779045
10	34.19254005089955	45.652975715855256	8.483296943292357	11.671187289952833
11	22.910102184127844	48.38995690667726	15.123898185259439	13.576042723935458
12	30.758465915653595	36.99508469403071	17.468447239834457	14.778002150481237
13	21.947909697643677	37.53571813183778	22.76111970100834	17.755252469510204
14	25.908556002627	39.972111818328415	17.859372811645407	16.259959367399173
15	22.592607678903132	39.745066977948916	18.208058780564425	19.454266562583523
16	26.0458765610379	41.281985897440904	17.773275444059866	14.898862097461327
17	27.559192637698594	38.65040841845887	16.75706983260953	17.033329111233
18	24.087510190234447	40.68901329515215	18.730278653042117	16.49319786157128
19	26.336810893242053	38.56888654286427	18.44710033774943	16.647202226144252
20	23.511332991473402	35.939373615146444	21.145245645331297	19.404047748048857
21	23.552233425977747	35.9765913365849	24.014413915745106	16.45676132169224
22	21.250035571660295	38.62741936113856	21.497446931267742	18.625098135933406
23	21.80127075920246	42.69893764887786	20.655500606252687	14.84429098566699
24	23.682858142450687	43.15358531646502	19.135265492922773	14.028291048161517
25	28.42931729753587	40.62166428499289	16.05830292770109	14.890715489770148
26	29.78020340851834	39.18155608018717	15.46560931882642	15.57263119246807
27	31.741303914779788	36.41885169658685	14.62980084892116	17.210043539712203
28	32.60841544574499	36.936775070487684	14.521551404257552	15.933258079509773
29	32.17943517225332	37.31263499793824	14.52093761874657	15.986992211061866
30	28.420612703016534	39.819502420825856	15.766085225792159	15.993799650365453
31	26.42798594096388	42.33088953702159	15.42359291066575	15.817531611348782
32	24.145987209825925	43.835780012577025	16.476569854092023	15.54166292350503
33	22.932923845399706	44.70361692641884	17.02233677071819	15.34112245746327
34	21.503194195597818	45.60303589473467	17.44852711006904	15.445242799598471
35	21.918615389165126	45.37180615314395	17.319074165935238	15.390504291755688
36	23.620754208476153	43.81541349334908	16.746468082874436	15.817364215300334
37	24.568550634793716	41.9768468945522	16.542189105083985	16.912413365570092
38	25.037984953327193	40.5778623189821	17.465434110962377	16.918718616728334
39	24.917627194492447	39.20086242444161	18.192825740155577	17.688684640910367
40	25.461162163805962	38.721551739049644	18.40011784681811	17.41716825032628
41	24.67758126101675	39.39013155655447	17.99847892790643	17.933808254522344
42	23.9404248623307	39.63648274118841	18.019012843182825	18.404079553298065
43	23.859963161709604	39.28723878544123	18.437391366939394	18.415406685909772
44	24.506502499501998	39.09942041908159	18.141100361184872	18.252976720231544
45	24.432904036867306	39.49905058541188	17.698951598548565	18.36909377917225
46	23.78764806877969	40.141349223310236	18.119227277520885	17.95177543038919
47	23.136477440313545	39.68686895177152	18.153264474038828	19.023389133876105
48	22.900728005414706	38.90730555414509	19.405610111167714	18.78635632927249
49	22.77479037829833	38.653812138110666	19.35198757698126	19.219409906609744
50	21.43751914592304	38.13739532864587	20.352792751974295	20.07229277345679
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	461.0
6	922.0
7	3233.0
8	5544.0
9	11198.0
10	16852.0
11	18059.5
12	19267.0
13	13450.0
14	7633.0
15	7910.0
16	8187.0
17	9255.0
18	10323.0
19	12311.5
20	14300.0
21	16642.0
22	18984.0
23	23380.0
24	27776.0
25	32758.5
26	37741.0
27	43336.0
28	48931.0
29	53079.0
30	57227.0
31	61171.5
32	65116.0
33	71948.0
34	78780.0
35	90575.0
36	102370.0
37	101570.5
38	100771.0
39	90471.5
40	80172.0
41	78014.0
42	75856.0
43	74221.0
44	72586.0
45	73232.5
46	73879.0
47	73592.5
48	73306.0
49	71588.5
50	69871.0
51	71739.0
52	73607.0
53	79157.0
54	84707.0
55	91823.5
56	98940.0
57	98643.5
58	98347.0
59	94308.0
60	90269.0
61	79761.5
62	69254.0
63	66312.0
64	63370.0
65	54808.5
66	46247.0
67	38413.5
68	30580.0
69	26462.0
70	22344.0
71	18023.5
72	13703.0
73	12792.0
74	11881.0
75	13369.5
76	14858.0
77	10165.5
78	5473.0
79	3448.5
80	1424.0
81	941.5
82	459.0
83	328.5
84	198.0
85	130.5
86	63.0
87	45.0
88	27.0
89	16.5
90	6.0
91	5.0
92	4.0
93	3.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1792157.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.28609702658777
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.79958491486971	21.501331196419766
2	9.451493759073298	5.157887515125255
3	3.4619231077060815	2.833871094463632
4	1.6369160896741266	1.7866020498892343
5	0.8997724478816033	1.2275639157373905
6	0.6146620249349513	1.0063036590560392
7	0.42595426392611735	0.8135840562063767
8	0.3412873495779508	0.7449919787624754
9	0.2630780088851185	0.6460534868400776
>10	2.9910460393410325	20.256031408078513
>50	0.7793176795695426	14.457678621449624
>100	0.30743300176659316	14.461480432864713
>500	0.017728496874859164	3.3581333948607037
>1k	0.007091398749943665	4.79672366305412
>5k	0.0014599938602825192	2.578000655071348
>10k+	0.001251423308813588	4.37376287212076
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TAAGGGGGGGAGTGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAAAAA	15624	0.8717986203217686	No Hit
TAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	14005	0.7814605528421895	No Hit
TAAGGGGGGGAGTGCAGTGCTTAAGGGGGAAAAAAAAAAAAAAAAAAAAA	13587	0.7581367034249789	No Hit
TAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	12409	0.6924058550673853	No Hit
TAAGGGGGGCAGAGTGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAAA	10869	0.6064758835302934	No Hit
TAAGGGGGGCAGAGTGCAGTGCTTAAGGGGGAAAAAAAAAAAAAAAAAAA	10359	0.5780185552939837	No Hit
TAAGGGGGGAGTGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAAAAAA	7759	0.43294197997162076	No Hit
TAAGGGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA	7334	0.40922753977469606	Illumina Single End Adapter 2 (97% over 34bp)
TAAGGGGGGAGAGTGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAAAA	6543	0.365090781667008	No Hit
TAAGGGGGGAGTGCAGTGCTTAAGGGGGAAAAAAAAAAAAAAAAAAAAAA	6409	0.35761375816962465	No Hit
TAAGGGGGGAGAGTGCAGTGCTTAAGGGGGAAAAAAAAAAAAAAAAAAAA	6175	0.34455686639061195	No Hit
TAAGGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA	5852	0.3265338918409492	Illumina Single End Adapter 2 (97% over 34bp)
TAAGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA	5227	0.2916597150807658	Illumina Single End Adapter 2 (100% over 34bp)
TAAGGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA	4795	0.267554684104127	Illumina Single End Adapter 2 (100% over 34bp)
TAAGGGGGGGTGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAAAAAAA	4791	0.26733148937286183	No Hit
TAAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4699	0.2621980105537629	No Hit
TAAGGGGGGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAA	4531	0.25282383184062557	No Hit
TAAGGGGGGGCAGAGTGCAGTGCTTAAGGGGGAAAAAAAAAAAAAAAAAA	3860	0.21538291567089266	No Hit
TAAGGGGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA	3786	0.21125381314248695	Illumina Single End Adapter 2 (97% over 34bp)
TAAGGGGGGCAGTGCTTAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA	3775	0.21064002763150771	No Hit
TAAGGGGGGGCAGAGTGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAA	3775	0.21064002763150771	No Hit
TAAGGGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC	3578	0.19964768711669795	No Hit
TAAGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA	3519	0.1963555648305366	Illumina Single End Adapter 2 (97% over 34bp)
TAAGGGGGGGTGCAGTGCTTAAGGGGGAAAAAAAAAAAAAAAAAAAAAAA	3430	0.19138948205988648	No Hit
TAAGGGGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAA	3074	0.17152515097728604	Illumina Single End Adapter 2 (100% over 34bp)
TAAGGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC	3055	0.17046497600377647	No Hit
TAAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	2694	0.15032165150709453	No Hit
TAAGGGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTAAGGGGGGA	2539	0.14167285567056903	No Hit
TAAGGGGGGGAGTGCAGTGCTTAAGGGGAAAAAAAAAAAAAAAAAAAAAA	2475	0.13810173997032626	No Hit
TAAGGGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA	2206	0.12309189429274334	Illumina Single End Adapter 2 (100% over 34bp)
TAAGGGGGGGAGTGCAGTGCTTAAGGGGGGTAAAAAAAAAAAAAAAAAAA	1958	0.10925382095430256	No Hit
TAAGGGGGGCAGAGTGCAGTGCTTAAGGGGAAAAAAAAAAAAAAAAAAAA	1873	0.1045109329149176	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.42574394988831893	0.0	0.0	0.0	0.0
8	0.780009787088966	5.579868281629344E-5	0.0	0.0	0.0
9	0.9514791393834358	5.579868281629344E-5	0.0	0.0	0.0
10	1.2574233172651725	5.579868281629344E-5	0.0	0.0	0.0
11	1.5858543643218759	5.579868281629344E-5	0.0	0.0	0.0
12	1.783437500174371	5.579868281629344E-5	0.0	0.0	0.0
13	1.9162941639599655	5.579868281629344E-5	0.0	0.0	0.0
14	2.0834112189947644	5.579868281629344E-5	0.0	0.0	0.0
15	2.259846654059884	5.579868281629344E-5	0.0	0.0	0.0
16	2.4378444522438603	5.579868281629344E-5	0.0	0.0	0.0
17	2.5907328431605046	5.579868281629344E-5	0.0	0.0	0.0
18	2.7624811888690557	5.579868281629344E-5	0.0	0.0	0.0
19	2.9388608252513593	5.579868281629344E-5	0.0	0.0	0.0
20	3.116468032655621	5.579868281629344E-5	0.0	0.0	0.0
21	3.2994319136102472	5.579868281629344E-5	0.0	0.0	0.0
22	3.5063892281758795	5.579868281629344E-5	0.0	0.0	0.0
23	3.736726190841539	5.579868281629344E-5	0.0	0.0	0.0
24	3.9613716878599363	5.579868281629344E-5	0.0	0.0	0.0
25	4.173239286513403	5.579868281629344E-5	0.0	0.0	0.0
26	4.378634237960179	5.579868281629344E-5	0.0	0.0	0.0
27	4.584196585455404	5.579868281629344E-5	0.0	0.0	0.0
28	4.776255651709086	5.579868281629344E-5	0.0	0.0	0.0
29	4.947669205320739	5.579868281629344E-5	0.0	0.0	0.0
30	5.112554313042886	5.579868281629344E-5	0.0	0.0	0.0
31	5.264159334254756	5.579868281629344E-5	0.0	0.0	0.0
32	5.413978797616504	5.579868281629344E-5	0.0	0.0	0.0
33	5.554200887533849	5.579868281629344E-5	0.0	0.0	0.0
34	5.691577244627563	5.579868281629344E-5	0.0	0.0	0.0
35	5.834031281857561	5.579868281629344E-5	0.0	0.0	0.0
36	5.957011578784671	5.579868281629344E-5	0.0	0.0	0.0
37	6.0885290741826745	5.579868281629344E-5	0.0	0.0	0.0
38	6.215526876272559	5.579868281629344E-5	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGAATT	20	7.85766E-4	44.000004	13
TATACTA	20	7.85766E-4	44.000004	30
CGTTTCG	20	7.85766E-4	44.000004	44
TGCGATG	50	2.7284841E-11	44.0	15
TCGCGAC	25	4.4438406E-5	44.0	23
TAATCGC	25	4.4438406E-5	44.0	28
CCGATAA	40	4.12625E-7	38.500004	23
ACATTCG	35	7.2924868E-6	37.714287	28
TTCGTAC	35	7.2924868E-6	37.714287	18
CAATTAG	35	7.2924868E-6	37.714287	38
ATATACG	35	7.2924868E-6	37.714287	37
CATTTCG	35	7.2924868E-6	37.714287	36
AGGGGGG	162290	0.0	37.161007	3
ACCGACC	30	1.3013858E-4	36.666664	38
GTTACGC	30	1.3013858E-4	36.666664	23
CTTATAC	30	1.3013858E-4	36.666664	20
TCGTACT	30	1.3013858E-4	36.666664	27
TCGTACA	30	1.3013858E-4	36.666664	19
TTACGCG	30	1.3013858E-4	36.666664	24
GTCCGAC	85	0.0	36.235294	10
>>END_MODULE