FastQCFastQC Report
Fri 17 Jun 2016
SRR1527845_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527845_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2594879
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAGGGGGGGAGTGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAAAAAAA202830.7816549442189791No Hit
TAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA186330.7180681642573701No Hit
TAAGGGGGGGAGTGCAGTGCTTAAGGGGGAAAAAAAAAAAAAAAAAAAAAAA176880.6816502811884484No Hit
TAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA165400.637409297312129No Hit
TAAGGGGGGCAGAGTGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAAAAA151950.5855764372828174No Hit
TAAGGGGGGCAGAGTGCAGTGCTTAAGGGGGAAAAAAAAAAAAAAAAAAAAA143260.55208739983637No Hit
TAAGGGGGGAGTGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAA98770.3806343185944316No Hit
TAAGGGGGGAGAGTGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAAAAAA85300.32872438368031803No Hit
TAAGGGGGGAGTGCAGTGCTTAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAA82730.31882026098326743No Hit
TAAGGGGGGAGAGTGCAGTGCTTAAGGGGGAAAAAAAAAAAAAAAAAAAAAA78600.3029042972716647No Hit
TAAGGGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA66500.25627399196648476Illumina Single End Adapter 2 (97% over 34bp)
TAAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA61820.23823846892282838No Hit
TAAGGGGGGGTGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAA59580.22960608182500997No Hit
TAAGGGGGGGCAGAGTGCAGTGCTTAAGGGGGAAAAAAAAAAAAAAAAAAAA54150.20868025060128045No Hit
TAAGGGGGGGCAGAGTGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAAAA51720.19931565209784347No Hit
TAAGGGGGGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA51150.1971190178809879No Hit
TAAGGGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT50460.1944599343553206No Hit
TAAGGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC47010.1811645167269842No Hit
TAAGGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA46660.17981570624295004Illumina Single End Adapter 2 (97% over 34bp)
TAAGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA43470.16752226211703897Illumina Single End Adapter 2 (100% over 34bp)
TAAGGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA42270.16289776902892197Illumina Single End Adapter 2 (100% over 34bp)
TAAGGGGGGGTGCAGTGCTTAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAA41540.16008453573365078No Hit
TAAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC41330.1592752494432303No Hit
TAAGGGGGGCAGTGCTTAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA40440.15584541706954352No Hit
TAAGGGGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA39530.1523385098110548Illumina Single End Adapter 2 (97% over 34bp)
TAAGGGGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA37060.14281976153801393Illumina Single End Adapter 2 (100% over 34bp)
TAAGGGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTAAGGGGGGAAG32290.12443740151274878No Hit
TAAGGGGGGGAGTGCAGTGCTTAAGGGGAAAAAAAAAAAAAAAAAAAAAAAA32040.12347396545272439No Hit
TAAGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA26670.10277935888340073Illumina Single End Adapter 2 (97% over 34bp)

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATACGA206.3129293E-446.09
CGTATTA301.8623141E-646.044
TTACGTA301.8623141E-646.041
TACGTAC206.3129293E-446.035
ACGCGTA206.3129293E-446.015
CCGATAA451.5897967E-840.8888923
TAATCGC402.9124567E-740.2528
CGTCGCA355.3757267E-639.4285739
TACGTAT355.3757267E-639.4285742
GTTATAG657.2759576E-1238.92307737
AGGGGGG2384000.038.6623
GATCGCG301.0019134E-438.33333244
TATTCGA301.0019134E-438.33333243
TAAGGGG3152450.037.0858541
ACACGAA504.0239684E-836.836
CACGCAT250.001892049936.828
ATAGACG250.001892049936.824
CGATTAG250.001892049936.833
CGACACG250.001892049936.845
TAACGCC250.001892049936.810
Produced by FastQC (version 0.11.5)