##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527845_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2594879
Sequences flagged as poor quality	0
Sequence length	52
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.74116134124173	31.0	31.0	33.0	30.0	34.0
2	31.4954126955438	31.0	31.0	34.0	30.0	34.0
3	32.27736476344369	34.0	31.0	34.0	30.0	34.0
4	35.998656199383475	37.0	35.0	37.0	35.0	37.0
5	36.058216972737455	37.0	35.0	37.0	35.0	37.0
6	36.17295103162807	37.0	37.0	37.0	35.0	37.0
7	36.468493135903444	37.0	37.0	37.0	35.0	37.0
8	36.4013007928308	37.0	37.0	37.0	35.0	37.0
9	34.565716166341474	35.0	35.0	35.0	33.0	38.0
10	33.32714935840939	35.0	33.0	35.0	26.0	39.0
11	32.824238047323206	35.0	33.0	35.0	22.0	39.0
12	33.27715396363376	35.0	33.0	35.0	25.0	39.0
13	33.39474981299706	35.0	33.0	35.0	27.0	39.0
14	33.94662101778156	35.0	33.0	35.0	29.0	40.0
15	34.01163060011661	35.0	34.0	35.0	29.0	40.0
16	34.1007280108244	35.0	34.0	35.0	29.0	40.0
17	34.13461359855315	35.0	34.0	35.0	30.0	40.0
18	34.10591592132042	35.0	34.0	35.0	29.0	40.0
19	34.13055213749851	35.0	34.0	35.0	29.0	40.0
20	34.17293137753244	35.0	34.0	35.0	30.0	40.0
21	34.12711999287828	35.0	34.0	35.0	30.0	39.0
22	33.97279834628127	35.0	34.0	35.0	30.0	39.0
23	33.70122576043045	35.0	34.0	35.0	29.0	37.0
24	33.491554712185035	35.0	33.0	35.0	29.0	37.0
25	33.38188370247707	35.0	33.0	35.0	29.0	37.0
26	33.15570860914902	35.0	33.0	35.0	27.0	37.0
27	32.99476237620328	35.0	33.0	35.0	25.0	36.0
28	32.79544518260774	35.0	33.0	35.0	24.0	36.0
29	32.55753081357551	35.0	33.0	35.0	23.0	36.0
30	32.150541123497476	35.0	33.0	35.0	22.0	36.0
31	31.82411125913771	35.0	33.0	35.0	21.0	36.0
32	31.5965580668694	35.0	32.0	35.0	20.0	36.0
33	31.324022815707398	35.0	32.0	35.0	18.0	36.0
34	30.971147402248814	35.0	31.0	35.0	17.0	36.0
35	30.585065816171003	35.0	31.0	35.0	15.0	36.0
36	30.207495609621873	35.0	30.0	35.0	11.0	35.0
37	29.747589772008638	35.0	29.0	35.0	10.0	35.0
38	29.385469996866906	34.0	27.0	35.0	9.0	35.0
39	29.084625525891575	34.0	25.0	35.0	9.0	35.0
40	28.79834358365072	34.0	24.0	35.0	8.0	35.0
41	28.567595252032948	34.0	23.0	35.0	8.0	35.0
42	28.410110452163664	34.0	23.0	35.0	8.0	35.0
43	28.32229402604129	34.0	23.0	35.0	8.0	35.0
44	28.213998417652615	34.0	23.0	35.0	8.0	35.0
45	28.142025119475704	34.0	23.0	35.0	8.0	35.0
46	27.973694341817094	34.0	23.0	35.0	8.0	35.0
47	27.769722595928364	34.0	23.0	35.0	8.0	35.0
48	27.513244355517156	33.0	20.0	35.0	8.0	35.0
49	27.460382160401313	34.0	20.0	35.0	8.0	35.0
50	27.299273299448643	34.0	20.0	35.0	8.0	35.0
51	27.1688795508384	34.0	18.0	35.0	8.0	35.0
52	26.646218956644994	33.0	17.0	35.0	8.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	17.0
13	48.0
14	177.0
15	809.0
16	2283.0
17	4866.0
18	8271.0
19	12676.0
20	18842.0
21	28135.0
22	40044.0
23	55305.0
24	73291.0
25	95509.0
26	114925.0
27	117720.0
28	100665.0
29	90822.0
30	100705.0
31	127492.0
32	170364.0
33	285444.0
34	701571.0
35	232600.0
36	67586.0
37	50927.0
38	71980.0
39	21804.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	0.0	100.0	0.0	0.0
3	0.0	100.0	0.0	0.0
4	100.0	0.0	0.0	0.0
5	100.0	0.0	0.0	0.0
6	100.0	0.0	0.0	0.0
7	98.01054307349206	1.4863506159632107	0.2978944297595379	0.20521188078519267
8	94.26385584838445	4.1900604999308255	0.9358817887076815	0.6102018629770406
9	79.74105921701937	14.467996388270898	3.6268743166829744	2.1640700780267594
10	34.54504044311893	45.282381182321025	8.38443719341056	11.788141181149488
11	23.150366548883397	47.65490028629466	15.3572478716734	13.837485293148543
12	30.848991417326204	36.311558265337226	17.78572334201325	15.05372697532332
13	21.909884815438406	36.88653690595978	22.9647316888379	18.238846589763916
14	25.91959008493267	39.19905321211509	18.237228017183075	16.644128685769164
15	22.774819172685895	38.894453267377784	18.618209172759116	19.712518387177205
16	26.25710100548041	40.480307559620314	18.067008134097968	15.19558330080131
17	27.615276088017975	37.94296381449771	16.982525967492126	17.459234129992186
18	24.20613832090051	39.98695122200303	18.989401817965305	16.81750863913115
19	26.46308363511362	37.99560596081744	18.673548940046917	16.86776146402202
20	23.71794600056496	35.38673672259863	21.332015866635786	19.56330141020063
21	23.93845724598334	35.27875480899109	24.115690943585424	16.667097001440144
22	21.48947985628617	37.921190159541155	21.57884818521403	19.010481798958644
23	21.953971649545124	41.95078074931432	20.87607938558985	15.219168215550704
24	23.796909220044558	42.415966216536496	19.356008507525786	14.431116055893165
25	28.41531339226222	39.90752555321462	16.398799327444554	15.278361727078604
26	29.687935352669626	38.662573476451115	15.714374350403236	15.935116820476022
27	31.579314488267084	35.87724128947824	14.902929963208303	17.640514259046373
28	32.52413696361179	36.470332528029246	14.817685140617346	16.18784536774162
29	32.01625201020934	36.868270158261716	14.845817473570058	16.269660357958887
30	28.439322218878026	39.23824579103689	16.024831986385493	16.297600003699593
31	26.649489244007142	41.60775897450325	15.64254055776782	16.1002112237218
32	24.506653296743316	43.169642977572366	16.589405517559776	15.734298208124542
33	23.320316669871698	44.00139659691261	17.133361517049543	15.544925216166149
34	21.463120245683903	45.23112638392773	17.54829415938084	15.757459211007527
35	22.012587099437006	44.971615246799566	17.514458284952784	15.501339368810646
36	23.628423521867497	43.50642168671449	16.86460139374514	16.000553397672878
37	24.54600002543471	41.723255689378966	16.66374424395126	17.067000041235065
38	24.946827963847255	40.32403823068436	17.614424410540916	17.11470939492747
39	24.94031513608149	38.83645441656432	18.2382685281279	17.98496191922629
40	25.344071920116505	38.33943702191894	18.562985017798518	17.753506040166034
41	24.65375071438784	38.978079517387904	18.20354629252462	18.16462347569964
42	24.13133714520022	39.040163337095876	18.168014770630926	18.660484747072985
43	24.01711216592373	38.69625520110957	18.646534192923834	18.64009844004287
44	24.6837713820182	38.467188643478174	18.278925529860928	18.570114444642698
45	24.882932884346438	38.75602677427349	17.871700376009827	18.489339965370256
46	23.97052039806095	39.640807914357474	18.225859471674788	18.162812215906794
47	23.401515061010553	39.04077993617429	18.265707187117393	19.291997815697766
48	23.313148705585114	38.206020396326764	19.522798558237206	18.958032339850913
49	22.9529392314632	38.07256523329219	19.52106437332916	19.45343116191545
50	21.364387318252604	37.86920314974224	20.23951791201054	20.52689161999461
51	20.992809298622404	37.21738084897215	20.885906433402095	20.903903419003353
52	21.61642219155498	35.577188762944246	21.750532491110373	21.0558565543904
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	577.0
6	1154.0
7	4284.0
8	7414.0
9	14877.0
10	22340.0
11	24057.0
12	25774.0
13	17966.0
14	10303.5
15	10449.0
16	11394.5
17	12340.0
18	14324.5
19	16309.0
20	17783.0
21	19257.0
22	23288.0
23	27319.0
24	33120.0
25	38921.0
26	46138.0
27	53355.0
28	61625.0
29	69895.0
30	78939.0
31	87983.0
32	100243.5
33	112504.0
34	130337.5
35	148171.0
36	147270.5
37	146370.0
38	128645.5
39	108087.0
40	105253.0
41	102948.5
42	100644.0
43	102134.5
44	103625.0
45	104165.5
46	104706.0
47	101247.5
48	97789.0
49	95868.5
50	93948.0
51	98330.0
52	102712.0
53	111922.0
54	121132.0
55	129395.0
56	137658.0
57	137830.0
58	138002.0
59	134632.0
60	131262.0
61	118017.5
62	104773.0
63	97660.5
64	80429.0
65	70310.0
66	61634.0
67	52958.0
68	43850.5
69	34743.0
70	29563.5
71	24384.0
72	22613.0
73	20842.0
74	21949.5
75	23057.0
76	17139.5
77	11222.0
78	7024.5
79	2827.0
80	2108.0
81	1389.0
82	862.5
83	336.0
84	203.5
85	71.0
86	53.5
87	36.0
88	23.0
89	7.0
90	4.0
91	2.5
92	1.0
93	2.0
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	2594879.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.143322434068185
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.82722019114085	22.747465192887038
2	8.467336409040898	4.765979574347262
3	3.385104276497228	2.8580424337921375
4	1.6851183019053053	1.8969931084028189
5	0.942281010495683	1.32594591509398
6	0.5660874766975543	0.9558949429552401
7	0.40899232452671674	0.805728200355011
8	0.2877193453150561	0.6477902644576504
9	0.22956163172485633	0.5814564318111104
>10	1.9509814916610988	12.721206200297983
>50	0.8118144110110637	16.20061576011649
>100	0.4095083174682755	19.9040416145812
>500	0.01861999848588629	3.5814056813413138
>1k	0.007310073479644247	4.146813155051728
>5k	0.001517185061812957	2.8754809033035835
>10k+	8.275554882616129E-4	3.9851406212055194
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TAAGGGGGGGAGTGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAAAAAAA	20283	0.7816549442189791	No Hit
TAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	18633	0.7180681642573701	No Hit
TAAGGGGGGGAGTGCAGTGCTTAAGGGGGAAAAAAAAAAAAAAAAAAAAAAA	17688	0.6816502811884484	No Hit
TAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	16540	0.637409297312129	No Hit
TAAGGGGGGCAGAGTGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAAAAA	15195	0.5855764372828174	No Hit
TAAGGGGGGCAGAGTGCAGTGCTTAAGGGGGAAAAAAAAAAAAAAAAAAAAA	14326	0.55208739983637	No Hit
TAAGGGGGGAGTGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAA	9877	0.3806343185944316	No Hit
TAAGGGGGGAGAGTGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAAAAAA	8530	0.32872438368031803	No Hit
TAAGGGGGGAGTGCAGTGCTTAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAA	8273	0.31882026098326743	No Hit
TAAGGGGGGAGAGTGCAGTGCTTAAGGGGGAAAAAAAAAAAAAAAAAAAAAA	7860	0.3029042972716647	No Hit
TAAGGGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA	6650	0.25627399196648476	Illumina Single End Adapter 2 (97% over 34bp)
TAAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	6182	0.23823846892282838	No Hit
TAAGGGGGGGTGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAA	5958	0.22960608182500997	No Hit
TAAGGGGGGGCAGAGTGCAGTGCTTAAGGGGGAAAAAAAAAAAAAAAAAAAA	5415	0.20868025060128045	No Hit
TAAGGGGGGGCAGAGTGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAAAA	5172	0.19931565209784347	No Hit
TAAGGGGGGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA	5115	0.1971190178809879	No Hit
TAAGGGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	5046	0.1944599343553206	No Hit
TAAGGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	4701	0.1811645167269842	No Hit
TAAGGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA	4666	0.17981570624295004	Illumina Single End Adapter 2 (97% over 34bp)
TAAGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA	4347	0.16752226211703897	Illumina Single End Adapter 2 (100% over 34bp)
TAAGGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA	4227	0.16289776902892197	Illumina Single End Adapter 2 (100% over 34bp)
TAAGGGGGGGTGCAGTGCTTAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAA	4154	0.16008453573365078	No Hit
TAAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	4133	0.1592752494432303	No Hit
TAAGGGGGGCAGTGCTTAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4044	0.15584541706954352	No Hit
TAAGGGGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA	3953	0.1523385098110548	Illumina Single End Adapter 2 (97% over 34bp)
TAAGGGGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA	3706	0.14281976153801393	Illumina Single End Adapter 2 (100% over 34bp)
TAAGGGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTAAGGGGGGAAG	3229	0.12443740151274878	No Hit
TAAGGGGGGGAGTGCAGTGCTTAAGGGGAAAAAAAAAAAAAAAAAAAAAAAA	3204	0.12347396545272439	No Hit
TAAGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA	2667	0.10277935888340073	Illumina Single End Adapter 2 (97% over 34bp)
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.40329433472620496	0.0	0.0	0.0	0.0
8	0.7184920761237807	1.1561232720292546E-4	0.0	0.0	0.0
9	0.8819293693463164	1.1561232720292546E-4	0.0	0.0	0.0
10	1.1751993060177373	1.1561232720292546E-4	0.0	0.0	0.0
11	1.4840383694191521	1.1561232720292546E-4	0.0	0.0	0.0
12	1.6606554679428212	1.1561232720292546E-4	0.0	0.0	0.0
13	1.7860177680731935	1.1561232720292546E-4	0.0	0.0	0.0
14	1.9409382865251135	1.1561232720292546E-4	0.0	0.0	0.0
15	2.10125404691317	1.1561232720292546E-4	0.0	0.0	3.8537442400975154E-5
16	2.2670421241221654	1.1561232720292546E-4	0.0	0.0	3.8537442400975154E-5
17	2.4137541673426774	1.1561232720292546E-4	0.0	0.0	3.8537442400975154E-5
18	2.571064007223458	1.1561232720292546E-4	0.0	0.0	3.8537442400975154E-5
19	2.727757248025823	1.1561232720292546E-4	0.0	0.0	3.8537442400975154E-5
20	2.8866085856026427	1.1561232720292546E-4	0.0	0.0	3.8537442400975154E-5
21	3.036442161657634	1.1561232720292546E-4	0.0	0.0	3.8537442400975154E-5
22	3.211903136909274	1.1561232720292546E-4	0.0	0.0	3.8537442400975154E-5
23	3.3974609220699694	1.1561232720292546E-4	0.0	0.0	3.8537442400975154E-5
24	3.585947552853139	1.1561232720292546E-4	0.0	0.0	3.8537442400975154E-5
25	3.760483629487155	1.1561232720292546E-4	0.0	0.0	3.8537442400975154E-5
26	3.931320111650678	1.1561232720292546E-4	0.0	0.0	3.8537442400975154E-5
27	4.096029140472446	1.1561232720292546E-4	0.0	0.0	3.8537442400975154E-5
28	4.246594927933056	1.1561232720292546E-4	0.0	0.0	3.8537442400975154E-5
29	4.378739817925999	1.1561232720292546E-4	0.0	0.0	3.8537442400975154E-5
30	4.513620866329413	1.1561232720292546E-4	0.0	0.0	3.8537442400975154E-5
31	4.634589898026074	1.1561232720292546E-4	0.0	0.0	3.8537442400975154E-5
32	4.758718999999615	1.1561232720292546E-4	0.0	0.0	3.8537442400975154E-5
33	4.88500619874761	1.1561232720292546E-4	0.0	0.0	3.8537442400975154E-5
34	5.009019688393948	1.1561232720292546E-4	0.0	0.0	3.8537442400975154E-5
35	5.140201142326868	1.1561232720292546E-4	0.0	0.0	3.8537442400975154E-5
36	5.254734421142566	1.1561232720292546E-4	0.0	0.0	3.8537442400975154E-5
37	5.371194572078313	1.1561232720292546E-4	0.0	0.0	3.8537442400975154E-5
38	5.49042941886693	1.1561232720292546E-4	0.0	0.0	3.8537442400975154E-5
39	5.623576282362299	1.1561232720292546E-4	0.0	0.0	3.8537442400975154E-5
40	5.7628891366418245	1.1561232720292546E-4	0.0	0.0	3.8537442400975154E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATACGA	20	6.3129293E-4	46.0	9
CGTATTA	30	1.8623141E-6	46.0	44
TTACGTA	30	1.8623141E-6	46.0	41
TACGTAC	20	6.3129293E-4	46.0	35
ACGCGTA	20	6.3129293E-4	46.0	15
CCGATAA	45	1.5897967E-8	40.88889	23
TAATCGC	40	2.9124567E-7	40.25	28
CGTCGCA	35	5.3757267E-6	39.42857	39
TACGTAT	35	5.3757267E-6	39.42857	42
GTTATAG	65	7.2759576E-12	38.923077	37
AGGGGGG	238400	0.0	38.662	3
GATCGCG	30	1.0019134E-4	38.333332	44
TATTCGA	30	1.0019134E-4	38.333332	43
TAAGGGG	315245	0.0	37.085854	1
ACACGAA	50	4.0239684E-8	36.8	36
CACGCAT	25	0.0018920499	36.8	28
ATAGACG	25	0.0018920499	36.8	24
CGATTAG	25	0.0018920499	36.8	33
CGACACG	25	0.0018920499	36.8	45
TAACGCC	25	0.0018920499	36.8	10
>>END_MODULE