FastQCFastQC Report
Fri 17 Jun 2016
SRR1527844_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527844_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2620409
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAGGGGGGGAGTGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAAAAAAA193780.7395028791307006No Hit
TAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA177100.6758486938489373No Hit
TAAGGGGGGGAGTGCAGTGCTTAAGGGGGAAAAAAAAAAAAAAAAAAAAAAA167310.638488113878406No Hit
TAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA156710.5980364133995876No Hit
TAAGGGGGGCAGAGTGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAAAAA141840.5412895467844905No Hit
TAAGGGGGGCAGAGTGCAGTGCTTAAGGGGGAAAAAAAAAAAAAAAAAAAAA134000.5113705532227985No Hit
TAAGGGGGGAGTGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAA92860.3543721609870825No Hit
TAAGGGGGGAGAGTGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAAAAAA81920.31262295313441524No Hit
TAAGGGGGGAGTGCAGTGCTTAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAA78720.3004111190276022No Hit
TAAGGGGGGAGAGTGCAGTGCTTAAGGGGGAAAAAAAAAAAAAAAAAAAAAA74550.28449757270716136No Hit
TAAGGGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA64700.2469080208471273Illumina Single End Adapter 2 (97% over 34bp)
TAAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA58900.22477407152852857No Hit
TAAGGGGGGGTGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAA57770.22046176760956016No Hit
TAAGGGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT51630.19703031091711257No Hit
TAAGGGGGGGCAGAGTGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAAAA50580.1930233028508145No Hit
TAAGGGGGGGCAGAGTGCAGTGCTTAAGGGGGAAAAAAAAAAAAAAAAAAAA50470.19260352105339282No Hit
TAAGGGGGGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA49020.18707003372374312No Hit
TAAGGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA45590.17398047404050285Illumina Single End Adapter 2 (97% over 34bp)
TAAGGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC44510.1698589800294534No Hit
TAAGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA42130.16077642841251116Illumina Single End Adapter 2 (100% over 34bp)
TAAGGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA41580.15867751942540267Illumina Single End Adapter 2 (100% over 34bp)
TAAGGGGGGGTGCAGTGCTTAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAA39270.14986210167954697No Hit
TAAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC39190.14955680582687664No Hit
TAAGGGGGGCAGTGCTTAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA38060.14524450190790827No Hit
TAAGGGGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA36600.13967285259667478Illumina Single End Adapter 2 (97% over 34bp)
TAAGGGGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA34420.1313535406114084Illumina Single End Adapter 2 (100% over 34bp)
TAAGGGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTAAGGGGGGAAG29880.11402800097236729No Hit
TAAGGGGGGGAGTGCAGTGCTTAAGGGGAAAAAAAAAAAAAAAAAAAAAAAA29340.11196725396684258No Hit
TAAGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA26860.10250308253406243Illumina Single End Adapter 2 (97% over 34bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGGTA351.0202348E-746.00000430
CCGATAA551.8189894E-1246.023
ATATCGC206.3129416E-446.022
ACGTATC206.3129416E-446.010
TATACGA253.418362E-546.045
TAATCGC501.6370905E-1146.028
TGCGATA402.912475E-740.2541
GCGATAT402.912475E-740.2542
CGGTATA355.375745E-639.42857434
AGGGGGG2394850.038.7739943
CAATCGA301.0019161E-438.33333212
ATCGCAT601.2369128E-1038.33333229
GACTACG301.0019161E-438.33333221
TAAGGGG3174150.037.2163541
CGACGTT250.001892053236.80000317
GACGTAA250.001892053236.8000039
CGCATAT250.001892053236.80000345
ATATCGT250.001892053236.80000336
CGAATAG250.001892053236.80000315
ACGTATA250.001892053236.80000318
Produced by FastQC (version 0.11.5)