##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527844_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2620409
Sequences flagged as poor quality	0
Sequence length	52
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.6837558564331	31.0	31.0	33.0	30.0	34.0
2	31.445525870198125	31.0	31.0	34.0	30.0	34.0
3	32.25064865828197	34.0	31.0	34.0	30.0	34.0
4	35.98384107213798	37.0	35.0	37.0	35.0	37.0
5	36.065915282690604	37.0	35.0	37.0	35.0	37.0
6	36.05834661688309	37.0	36.0	37.0	35.0	37.0
7	36.41327060012387	37.0	37.0	37.0	35.0	37.0
8	36.365873418996806	37.0	37.0	37.0	35.0	37.0
9	34.52828050888239	35.0	35.0	35.0	33.0	38.0
10	33.2994929417507	35.0	33.0	35.0	26.0	39.0
11	32.814781204002884	35.0	33.0	35.0	22.0	39.0
12	33.22734237288912	35.0	33.0	35.0	25.0	39.0
13	33.1885991843258	35.0	33.0	35.0	27.0	39.0
14	33.8590903175802	35.0	33.0	35.0	29.0	40.0
15	33.96124727094129	35.0	34.0	35.0	29.0	40.0
16	34.075859531851705	35.0	34.0	35.0	29.0	40.0
17	34.150165489433135	35.0	34.0	35.0	30.0	40.0
18	34.1316328863166	35.0	34.0	35.0	30.0	40.0
19	34.1064387276948	35.0	34.0	35.0	29.0	40.0
20	34.15234148562305	35.0	34.0	35.0	30.0	40.0
21	34.16988760151564	35.0	34.0	35.0	30.0	40.0
22	33.91800020531146	35.0	34.0	35.0	30.0	39.0
23	33.66196002227133	35.0	34.0	35.0	29.0	38.0
24	33.504587642616094	35.0	33.0	35.0	29.0	37.0
25	33.3642179522357	35.0	33.0	35.0	29.0	37.0
26	33.0923550483913	35.0	33.0	35.0	27.0	37.0
27	32.92344095902586	35.0	33.0	35.0	25.0	37.0
28	32.74891324216945	35.0	33.0	35.0	23.0	36.0
29	32.517921438981475	35.0	33.0	35.0	23.0	36.0
30	32.09419254780456	35.0	33.0	35.0	22.0	36.0
31	31.787925472702927	35.0	32.0	35.0	21.0	36.0
32	31.57112420236688	35.0	32.0	35.0	20.0	36.0
33	31.055008588353957	35.0	31.0	35.0	18.0	36.0
34	30.863820495197505	35.0	31.0	35.0	17.0	36.0
35	30.58558759338714	35.0	31.0	35.0	15.0	36.0
36	30.093023646308648	35.0	30.0	35.0	10.0	35.0
37	29.69791624131958	34.0	29.0	35.0	10.0	35.0
38	29.112125626190416	34.0	25.0	35.0	9.0	35.0
39	28.94190525219536	34.0	25.0	35.0	9.0	35.0
40	28.725451637511547	34.0	24.0	35.0	8.0	35.0
41	28.346133370782958	34.0	23.0	35.0	8.0	35.0
42	28.29498791982473	34.0	23.0	35.0	8.0	35.0
43	28.30743101553994	34.0	23.0	35.0	8.0	35.0
44	28.19198644181118	34.0	23.0	35.0	8.0	35.0
45	28.11770185493944	34.0	23.0	35.0	8.0	35.0
46	27.820713484040088	34.0	23.0	35.0	8.0	35.0
47	27.56561094088747	34.0	20.0	35.0	8.0	35.0
48	27.420427498150097	33.0	20.0	35.0	8.0	35.0
49	27.258377604412136	33.0	20.0	35.0	8.0	35.0
50	26.98134756826129	33.0	18.0	35.0	8.0	35.0
51	26.890944886847816	33.0	18.0	35.0	8.0	35.0
52	26.51922886847053	33.0	17.0	35.0	8.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	8.0
12	10.0
13	49.0
14	204.0
15	840.0
16	2324.0
17	4954.0
18	8232.0
19	13122.0
20	19416.0
21	29319.0
22	41850.0
23	56680.0
24	75298.0
25	97409.0
26	116153.0
27	118518.0
28	102549.0
29	93458.0
30	105904.0
31	133891.0
32	182268.0
33	315196.0
34	683546.0
35	208167.0
36	67953.0
37	53364.0
38	70752.0
39	18972.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	0.0	100.0	0.0	0.0
3	0.0	100.0	0.0	0.0
4	100.0	0.0	0.0	0.0
5	100.0	0.0	0.0	0.0
6	100.0	0.0	0.0	0.0
7	97.99943443943293	1.4924006137973118	0.3006400909171049	0.20752485585265507
8	94.23929623200043	4.208961272839469	0.9361134082503916	0.6156290869097153
9	79.74518481656871	14.439272647895807	3.647865657612991	2.167676877922492
10	34.63787523245417	45.140586832055604	8.51237345009882	11.709164485391403
11	23.17874805039977	47.576008172769974	15.387750538179345	13.857493238650914
12	30.8192728692353	36.27452813663821	17.85080115356038	15.055397840566108
13	22.201076244204625	36.662292031511114	22.947944385781	18.188687338503264
14	25.87233519652848	39.20063623655696	18.2750097408458	16.652018826068755
15	22.815140689869406	38.842218905522	18.625794675564006	19.71684572904459
16	26.19522372270894	40.420102358067005	18.088016031085225	15.296657888138837
17	27.66800907797218	37.86359304978726	17.031959514716977	17.436438357523578
18	24.274493027615154	39.93151450784973	18.97394643355293	16.820046030982187
19	26.48014107721352	37.946900655584685	18.668688742864187	16.90426952433761
20	23.72171672437394	35.34696301226259	21.287554729051838	19.643765534311626
21	23.91176339266122	35.23919357626996	24.168555366738552	16.68048766433026
22	21.68562235895236	37.76399027785357	21.595979864212037	18.95440749898203
23	21.992177557014955	41.90819066794535	20.858194274252607	15.241437500787091
24	23.826700335710953	42.35670843749964	19.344079492934117	14.472511733855287
25	28.487384984557757	39.8232871280781	16.374085114194006	15.315242773170143
26	29.736770099629485	38.543067131886666	15.767347768993314	15.952814999490538
27	31.593655799533582	35.85310537400841	14.885653346481408	17.6675854799766
28	32.54904100848379	36.41099538278185	14.829593395534818	16.210370213199543
29	32.05404194536044	36.7906307755774	14.901452406857096	16.253874872205067
30	28.48368327234413	39.18590571166562	16.08634377305222	16.24406724293803
31	26.63065193257999	41.59488079914242	15.611265264315607	16.163202003961977
32	24.46835589406081	43.101363184144155	16.634426152558625	15.795854769236406
33	23.309223865434745	43.867197830567676	17.23643141204293	15.587146891954653
34	21.488515724072084	45.16218651363203	17.57500451265432	15.774293249641563
35	22.067318498753437	44.90112039761732	17.499024007320997	15.53253709630825
36	23.898979128830653	43.25454537822149	16.918236809597282	15.928238683350576
37	24.57311053350832	41.690285753101904	16.69456943553468	17.042034277855098
38	25.060286390406993	39.989978663636094	17.54054424328416	17.40919070267275
39	24.9411446839024	38.839127785013716	18.269438091534564	17.950289439549323
40	25.420077552778974	38.341648193087416	18.51592633058427	17.72234792354934
41	25.013537962966847	38.681709610980576	18.201395278370665	18.10335714768191
42	24.09303280518423	39.03734111735992	18.153540153464593	18.716085923991255
43	24.074982187895095	38.677664440932695	18.60598860712202	18.64136476405019
44	24.713622949699836	38.45724083530472	18.262378124941563	18.56675809005388
45	24.863103431563545	38.78886845526786	17.845992743880824	18.50203536928777
46	24.096696355416274	39.496925861573516	18.25054791065059	18.155829872359618
47	23.597232340447615	38.930105949109475	18.29947157104101	19.173190139401903
48	23.287967641692575	38.17613967895851	19.537637063527104	18.998255615821805
49	23.08761723837767	38.02040063211506	19.433950959563944	19.458031169943318
50	21.48485217384004	37.86763821983515	20.196389189626505	20.45112041669831
51	21.019123350591453	37.1435527812643	20.877122617118168	20.96020125102608
52	21.63437081768533	35.55372462848357	21.771181521663223	21.04072303216788
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	567.0
6	1134.0
7	4171.5
8	7209.0
9	14534.0
10	21859.0
11	23777.0
12	25695.0
13	18269.0
14	10874.5
15	10906.0
16	11577.0
17	12248.0
18	14166.5
19	16085.0
20	17771.5
21	19458.0
22	23362.0
23	27266.0
24	32750.5
25	38235.0
26	45554.5
27	52874.0
28	61506.0
29	70138.0
30	79109.5
31	88081.0
32	100971.5
33	113862.0
34	130988.0
35	148114.0
36	147666.5
37	147219.0
38	130389.0
39	110412.0
40	107265.0
41	104665.0
42	102065.0
43	103531.0
44	104997.0
45	105243.0
46	105489.0
47	102277.5
48	99066.0
49	97278.5
50	95491.0
51	100137.5
52	104784.0
53	113633.0
54	122482.0
55	130541.5
56	138601.0
57	139324.5
58	140048.0
59	135913.0
60	131778.0
61	119176.5
62	106575.0
63	99198.5
64	81649.0
65	71476.0
66	62428.5
67	53381.0
68	44273.0
69	35165.0
70	29881.5
71	24598.0
72	22659.5
73	20721.0
74	22168.5
75	23616.0
76	17420.0
77	11224.0
78	7142.5
79	3061.0
80	2236.5
81	1412.0
82	879.0
83	346.0
84	223.0
85	100.0
86	70.0
87	40.0
88	25.0
89	8.0
90	6.0
91	4.0
92	2.0
93	2.5
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	2620409.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.169951508478604
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.76531128330062	23.559202137010583
2	8.68119015287146	5.064597915902849
3	3.361632804547167	2.941759976938554
4	1.64999393577362	1.9252097238320103
5	0.9384676975965265	1.368752861558212
6	0.5569862279576471	0.9748356756248981
7	0.39443866566555663	0.8054029725373304
8	0.2870873956850515	0.6699460328662689
9	0.22784856299330972	0.5981698380412239
>10	1.9569383309595128	13.293211373722771
>50	0.7747802611218229	15.852560950041934
>100	0.38023811447793904	19.146399277845642
>500	0.01654357394635786	3.3766818667016385
>1k	0.006373344061301799	3.964513898436205
>5k	0.0013560306513408082	2.6189596138312146
>10k+	8.136183908044849E-4	3.8397958851087646
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TAAGGGGGGGAGTGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAAAAAAA	19378	0.7395028791307006	No Hit
TAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	17710	0.6758486938489373	No Hit
TAAGGGGGGGAGTGCAGTGCTTAAGGGGGAAAAAAAAAAAAAAAAAAAAAAA	16731	0.638488113878406	No Hit
TAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	15671	0.5980364133995876	No Hit
TAAGGGGGGCAGAGTGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAAAAA	14184	0.5412895467844905	No Hit
TAAGGGGGGCAGAGTGCAGTGCTTAAGGGGGAAAAAAAAAAAAAAAAAAAAA	13400	0.5113705532227985	No Hit
TAAGGGGGGAGTGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAA	9286	0.3543721609870825	No Hit
TAAGGGGGGAGAGTGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAAAAAA	8192	0.31262295313441524	No Hit
TAAGGGGGGAGTGCAGTGCTTAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAA	7872	0.3004111190276022	No Hit
TAAGGGGGGAGAGTGCAGTGCTTAAGGGGGAAAAAAAAAAAAAAAAAAAAAA	7455	0.28449757270716136	No Hit
TAAGGGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA	6470	0.2469080208471273	Illumina Single End Adapter 2 (97% over 34bp)
TAAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5890	0.22477407152852857	No Hit
TAAGGGGGGGTGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAA	5777	0.22046176760956016	No Hit
TAAGGGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	5163	0.19703031091711257	No Hit
TAAGGGGGGGCAGAGTGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAAAA	5058	0.1930233028508145	No Hit
TAAGGGGGGGCAGAGTGCAGTGCTTAAGGGGGAAAAAAAAAAAAAAAAAAAA	5047	0.19260352105339282	No Hit
TAAGGGGGGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA	4902	0.18707003372374312	No Hit
TAAGGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA	4559	0.17398047404050285	Illumina Single End Adapter 2 (97% over 34bp)
TAAGGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	4451	0.1698589800294534	No Hit
TAAGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA	4213	0.16077642841251116	Illumina Single End Adapter 2 (100% over 34bp)
TAAGGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA	4158	0.15867751942540267	Illumina Single End Adapter 2 (100% over 34bp)
TAAGGGGGGGTGCAGTGCTTAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAA	3927	0.14986210167954697	No Hit
TAAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	3919	0.14955680582687664	No Hit
TAAGGGGGGCAGTGCTTAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3806	0.14524450190790827	No Hit
TAAGGGGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA	3660	0.13967285259667478	Illumina Single End Adapter 2 (97% over 34bp)
TAAGGGGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA	3442	0.1313535406114084	Illumina Single End Adapter 2 (100% over 34bp)
TAAGGGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTAAGGGGGGAAG	2988	0.11402800097236729	No Hit
TAAGGGGGGGAGTGCAGTGCTTAAGGGGAAAAAAAAAAAAAAAAAAAAAAAA	2934	0.11196725396684258	No Hit
TAAGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA	2686	0.10250308253406243	Illumina Single End Adapter 2 (97% over 34bp)
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.3928776004051276	0.0	0.0	0.0	0.0
8	0.71443045723015	0.0	0.0	0.0	0.0
9	0.872497384950212	0.0	0.0	0.0	0.0
10	1.15615539406253	0.0	0.0	0.0	0.0
11	1.4651529589464851	0.0	0.0	0.0	0.0
12	1.6407362362135072	0.0	0.0	0.0	0.0
13	1.7612899360367027	0.0	0.0	0.0	0.0
14	1.9141668342613691	0.0	0.0	0.0	0.0
15	2.073912889171118	0.0	0.0	0.0	7.632396316758186E-5
16	2.2388108115946785	0.0	0.0	0.0	7.632396316758186E-5
17	2.3830249399998245	0.0	0.0	0.0	7.632396316758186E-5
18	2.5443737981360925	0.0	0.0	0.0	7.632396316758186E-5
19	2.7029749935983274	0.0	0.0	0.0	7.632396316758186E-5
20	2.8561190256940807	0.0	0.0	0.0	7.632396316758186E-5
21	3.00811819834232	0.0	0.0	0.0	7.632396316758186E-5
22	3.180534031137887	0.0	0.0	0.0	7.632396316758186E-5
23	3.370580699425166	0.0	0.0	0.0	7.632396316758186E-5
24	3.554368802732703	0.0	0.0	0.0	7.632396316758186E-5
25	3.720144450732691	0.0	0.0	0.0	7.632396316758186E-5
26	3.887866359793452	0.0	0.0	0.0	7.632396316758186E-5
27	4.050589049266737	0.0	0.0	0.0	7.632396316758186E-5
28	4.195108473524553	0.0	0.0	0.0	7.632396316758186E-5
29	4.331728367594525	0.0	0.0	0.0	7.632396316758186E-5
30	4.459990787697645	0.0	0.0	0.0	7.632396316758186E-5
31	4.5805063255392575	0.0	0.0	0.0	7.632396316758186E-5
32	4.706593512692103	0.0	0.0	0.0	7.632396316758186E-5
33	4.828139424036476	0.0	0.0	0.0	7.632396316758186E-5
34	4.952051378239046	0.0	0.0	0.0	7.632396316758186E-5
35	5.0864960393587415	0.0	0.0	0.0	7.632396316758186E-5
36	5.195524820743632	0.0	0.0	0.0	7.632396316758186E-5
37	5.31046870927401	0.0	0.0	0.0	7.632396316758186E-5
38	5.426862753104572	0.0	0.0	0.0	7.632396316758186E-5
39	5.5520340526994065	0.0	0.0	0.0	7.632396316758186E-5
40	5.685066720500502	0.0	0.0	0.0	1.1448594475137278E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CACGGTA	35	1.0202348E-7	46.000004	30
CCGATAA	55	1.8189894E-12	46.0	23
ATATCGC	20	6.3129416E-4	46.0	22
ACGTATC	20	6.3129416E-4	46.0	10
TATACGA	25	3.418362E-5	46.0	45
TAATCGC	50	1.6370905E-11	46.0	28
TGCGATA	40	2.912475E-7	40.25	41
GCGATAT	40	2.912475E-7	40.25	42
CGGTATA	35	5.375745E-6	39.428574	34
AGGGGGG	239485	0.0	38.773994	3
CAATCGA	30	1.0019161E-4	38.333332	12
ATCGCAT	60	1.2369128E-10	38.333332	29
GACTACG	30	1.0019161E-4	38.333332	21
TAAGGGG	317415	0.0	37.216354	1
CGACGTT	25	0.0018920532	36.800003	17
GACGTAA	25	0.0018920532	36.800003	9
CGCATAT	25	0.0018920532	36.800003	45
ATATCGT	25	0.0018920532	36.800003	36
CGAATAG	25	0.0018920532	36.800003	15
ACGTATA	25	0.0018920532	36.800003	18
>>END_MODULE