FastQCFastQC Report
Fri 17 Jun 2016
SRR1527843_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527843_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1753852
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTTTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC160000.9122776608288499No Hit
AGTTTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG97530.5560902516289857No Hit
AGTTTAGGGAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATACCGTC65000.3706127997117203No Hit
AGTTTAGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCG59060.3367444915534492No Hit
AGTTTAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC45650.2602842201052312No Hit
AGTTTAGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGA43860.25007811377470845No Hit
AGTTTAGGGAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGA36670.20908263639121202No Hit
AGTTTAGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGC35120.20024494655193256No Hit
AGTTTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC35010.19961775566011272No Hit
AGTTTAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT33740.1923765517272837No Hit
AGTTTAGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC29340.16728891605449034No Hit
AGTTTAGGGGGGGGCTGGTGAGATGGCTCAGCGGTTAGGAGCGCCGACTG26500.15109598757477827No Hit
AGTTTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTG25740.14676266868584123No Hit
AGTTTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT23550.13427586820324633No Hit
AGTTTAGGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTG23470.13381972937283193No Hit
AGTTTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGATTG23430.1335916599576247No Hit
AGTTTAGGGGGGGCTGGCAAGATGGCTCAGCGGGTAAGGGCACTGACTGC21990.12538116101016505No Hit
AGTTTAGGGGGAGGTTCGAAGACGATCAGATACCGTCGTAGTTCCGACCA20510.1169425926474982No Hit
AGTTTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG20310.11580224557146213No Hit
AGTTTAGGGGAGCTGGAGAGATGGCTCAGCGGTTAAGAGCACCAACTGCT17700.10092071622919152No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAA351.4466605E-744.032
CCGTATA408.3164196E-944.031
ACGATAA207.8576163E-444.029
AACGACG207.8576163E-444.013
TCGTATC207.8576163E-444.034
TCCGTAT302.5284826E-644.043
CGATGTA408.3164196E-944.021
TTATTCG207.8576163E-444.034
CAATACG207.8576163E-444.019
CGATAGG302.5284826E-644.027
TCGGTAC254.443801E-544.011
TCGAACG302.5284826E-644.026
TAGGGTA22550.042.731715
TAGGGAT233500.042.6903655
TAGGGAC118400.042.587845
TTAGGGA697750.042.584314
GTTTAGG1790400.042.5561872
TTTAGGG1755150.042.549753
AGTTTAG1805950.042.5442541
TAGGGGG321950.042.4693265
Produced by FastQC (version 0.11.5)