##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527843_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1753852
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.10649929412516	31.0	31.0	33.0	3.0	34.0
2	29.934633595081	33.0	31.0	34.0	16.0	34.0
3	29.957407466536516	31.0	30.0	31.0	25.0	33.0
4	35.18071251166005	35.0	35.0	37.0	33.0	37.0
5	36.10443982730584	37.0	35.0	37.0	35.0	37.0
6	36.14934954602783	37.0	35.0	37.0	35.0	37.0
7	36.440786337729755	37.0	36.0	37.0	35.0	37.0
8	36.673885253715824	37.0	37.0	37.0	35.0	37.0
9	38.76918805007492	39.0	39.0	39.0	39.0	39.0
10	38.32704070810992	39.0	39.0	39.0	37.0	39.0
11	38.149589018913794	39.0	38.0	39.0	37.0	39.0
12	37.73385895731225	39.0	37.0	39.0	35.0	39.0
13	37.63560950410867	39.0	37.0	39.0	35.0	39.0
14	38.392924830601444	40.0	38.0	40.0	35.0	40.0
15	38.39121088894616	40.0	38.0	40.0	35.0	40.0
16	38.43169321014544	40.0	38.0	40.0	35.0	40.0
17	38.31898130515003	40.0	38.0	40.0	35.0	40.0
18	38.23255554060434	40.0	38.0	40.0	34.0	40.0
19	38.21007986990921	40.0	38.0	40.0	34.0	40.0
20	38.13231276071185	40.0	38.0	40.0	34.0	40.0
21	38.102123212220874	40.0	38.0	40.0	34.0	40.0
22	38.144467720195316	40.0	38.0	40.0	34.0	40.0
23	38.12127477118936	40.0	38.0	40.0	34.0	40.0
24	38.079180569398105	40.0	38.0	40.0	34.0	40.0
25	38.081363193701634	40.0	38.0	40.0	34.0	40.0
26	37.99209625441599	40.0	38.0	40.0	34.0	40.0
27	37.90916565365835	40.0	37.0	40.0	34.0	40.0
28	37.76376341903422	40.0	37.0	40.0	34.0	40.0
29	37.828531711911836	40.0	37.0	40.0	34.0	40.0
30	38.012323160677184	40.0	38.0	40.0	34.0	40.0
31	38.06107470869834	40.0	38.0	40.0	34.0	40.0
32	38.05224557146213	40.0	38.0	40.0	35.0	40.0
33	37.9535844529641	40.0	37.0	40.0	34.0	40.0
34	37.60803135042181	40.0	37.0	40.0	33.0	40.0
35	37.5699870912711	40.0	37.0	40.0	33.0	40.0
36	37.64689209807897	40.0	37.0	40.0	33.0	40.0
37	37.616230445898516	40.0	36.0	40.0	34.0	40.0
38	37.55539064869784	40.0	36.0	40.0	34.0	40.0
39	37.50035578828772	40.0	36.0	40.0	34.0	40.0
40	37.32352159703327	40.0	35.0	40.0	33.0	40.0
41	37.27411320909632	40.0	35.0	40.0	33.0	40.0
42	37.181140141813565	40.0	35.0	40.0	33.0	40.0
43	37.06287417638433	40.0	35.0	40.0	33.0	40.0
44	36.76968695192069	40.0	35.0	40.0	32.0	40.0
45	36.773148475469995	39.0	35.0	40.0	32.0	40.0
46	36.603453427085064	39.0	35.0	40.0	32.0	40.0
47	36.60984792331394	39.0	35.0	40.0	32.0	40.0
48	36.60328351537074	39.0	35.0	40.0	32.0	40.0
49	36.53192686726132	39.0	35.0	40.0	32.0	40.0
50	36.012064872064464	38.0	35.0	40.0	31.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	2.0
14	1.0
15	13.0
16	25.0
17	52.0
18	111.0
19	267.0
20	473.0
21	735.0
22	1154.0
23	1793.0
24	2630.0
25	3786.0
26	5089.0
27	7516.0
28	10415.0
29	14194.0
30	19574.0
31	27134.0
32	37276.0
33	61468.0
34	116049.0
35	114082.0
36	149342.0
37	333676.0
38	620873.0
39	226121.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	100.0	0.0	0.0	0.0
3	0.0	0.0	100.0	0.0
4	0.0	0.0	100.0	0.0
5	0.0	0.0	100.0	0.0
6	0.0	100.0	0.0	0.0
7	99.58964610468843	0.31809981686025957	0.07400852523474045	0.018245553216576997
8	99.08623988797231	0.7973876929182167	0.08934619340742549	0.02702622570205468
9	97.8054020521686	1.0829306007576467	0.3862355546534143	0.7254317924203411
10	48.25350143569697	39.576771586199975	4.283713791129468	7.886013186973587
11	33.790536487685394	23.19916389752385	25.03734636674018	17.972953248050576
12	31.003813320622264	18.506236558158843	26.317043855467848	24.172906265751042
13	26.979984628121418	18.27064085224979	28.4119754688537	26.337399050775094
14	25.25709124829233	20.379484699963278	29.740080690959097	24.623343360785288
15	26.99247142860401	19.363378437861346	28.639018571692482	25.005131561842163
16	31.060830674424068	21.742769629364396	25.48031418842639	21.71608550778515
17	29.33713905164176	22.42794717000066	24.409813370797536	23.825100407560047
18	28.510615490930817	22.210939121430997	25.373406650048008	23.90503873759017
19	27.654328871535338	24.856943459311275	24.421216841557897	23.067510827595488
20	28.663593051181056	24.452519368795087	23.660776393903248	23.223111186120608
21	27.751543459767415	24.618781972481145	26.211960872411126	21.41771369534031
22	27.092593901879976	22.0971324832426	27.69196032504453	23.11831328983289
23	27.83456072690284	22.377429794532265	27.025484476455254	22.762525002109644
24	28.199984947418592	22.33563607419554	25.6198926705332	23.84448630785266
25	28.213498060269625	23.056563495665543	24.41374756820986	24.316190875854975
26	25.8948303505655	23.121905383122407	25.063460314781405	25.91980395153069
27	23.84830647055738	21.772247601279926	24.86561009708915	29.51383583107355
28	25.376713656568512	24.86555307973535	24.060354009346284	25.697379254349855
29	28.161498233602376	23.525759300100578	23.741855071009415	24.57088739528763
30	26.831169334698707	22.44379799435756	25.214556302356183	25.510476368587543
31	29.589269790153335	22.837616857066617	24.215498229040993	23.357615123739063
32	30.584450683410005	23.727771784620366	23.42044824762865	22.26732928434098
33	30.60680148610031	22.281697657499038	24.751974510962157	22.3595263454385
34	28.039823200589332	23.713916567646528	26.856428022432908	21.38983220933123
35	25.461156357548987	26.929239183237808	26.676481253834417	20.93312320537879
36	25.221683471581414	27.98212163854191	24.592154868255705	22.20404002162098
37	28.00430138917081	27.374487699076088	22.57311335278005	22.048097558973048
38	27.63910523807026	27.85166593304338	23.514926002878237	20.994302826008123
39	28.303357409861263	24.79097438096259	23.039059168048386	23.866609041127756
40	26.548249225134164	24.408045832829682	23.626565981622168	25.417138960413993
41	24.52065510658824	26.268351035321107	23.451750774865836	25.75924308322481
42	24.129230972738863	24.280498012375045	23.924253585821383	27.66601742906471
43	23.952477175953273	23.889473000002283	24.908430129794304	27.249619694250143
44	24.69364575802291	23.08632655435008	25.665734622989856	26.554293064637154
45	26.322118399956214	23.649258888435284	24.831285650100465	25.19733706150804
46	26.35889459315837	25.406476715253056	23.24090060050677	24.993728091081802
47	23.055195079174297	25.946944211940348	24.986771973917982	26.011088734967373
48	25.565270045591078	23.945521058789453	25.26353420927193	25.22567468634754
49	26.733099486159606	24.06183646054513	25.006214891564397	24.198849161730866
50	24.106880170048555	24.465063186631482	25.236108862093264	26.191947781226695
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	3.5
2	7.0
3	9.0
4	11.0
5	36.5
6	62.0
7	704.0
8	1346.0
9	2505.0
10	3664.0
11	4467.5
12	5271.0
13	4870.5
14	4470.0
15	4552.5
16	4635.0
17	4913.0
18	5191.0
19	5475.0
20	5759.0
21	7027.5
22	8296.0
23	10340.5
24	12385.0
25	14425.0
26	16465.0
27	18829.0
28	21193.0
29	23169.5
30	25146.0
31	27001.0
32	28856.0
33	33717.5
34	38579.0
35	44241.5
36	49904.0
37	53612.5
38	57321.0
39	60021.5
40	62722.0
41	67766.0
42	72810.0
43	78943.0
44	85076.0
45	89396.0
46	93716.0
47	104948.5
48	116181.0
49	113498.0
50	110815.0
51	118294.5
52	125774.0
53	129379.5
54	132985.0
55	138313.5
56	143642.0
57	139421.0
58	135200.0
59	131163.5
60	127127.0
61	109182.5
62	91238.0
63	73607.0
64	55976.0
65	45305.5
66	34635.0
67	30485.0
68	26335.0
69	21931.5
70	17528.0
71	18660.5
72	19793.0
73	14621.0
74	9449.0
75	6171.5
76	2894.0
77	1932.5
78	971.0
79	663.5
80	356.0
81	204.0
82	52.0
83	30.5
84	9.0
85	6.0
86	3.0
87	1.5
88	0.0
89	2.0
90	4.0
91	2.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1753852.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	7.785103929013629
#Duplication Level	Percentage of deduplicated	Percentage of total
1	64.7716564554142	5.04254077159766
2	9.5227219625646	1.4827076033133204
3	3.9555319818296324	0.9238268271924279
4	1.9836276313312886	0.6177098906550885
5	1.073986739017875	0.4180549190818296
6	0.646913372214619	0.3021772701455691
7	0.4849489848359352	0.26427647730521864
8	0.4176516459218755	0.26011691757003214
9	0.32526326089615754	0.22789874613298144
>10	9.359961641819478	21.456159643942552
>50	5.109007071681418	27.557953622566156
>100	2.2281671045322895	29.97586279199688
>500	0.0833516084527777	4.323128663058304
>1k	0.03423369632881941	4.93674560920262
>5k	0.002232632369270831	1.2838389113031743
>10k+	7.442107897569438E-4	0.9270013349361789
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AGTTTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	16000	0.9122776608288499	No Hit
AGTTTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG	9753	0.5560902516289857	No Hit
AGTTTAGGGAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATACCGTC	6500	0.3706127997117203	No Hit
AGTTTAGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCG	5906	0.3367444915534492	No Hit
AGTTTAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC	4565	0.2602842201052312	No Hit
AGTTTAGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGA	4386	0.25007811377470845	No Hit
AGTTTAGGGAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGA	3667	0.20908263639121202	No Hit
AGTTTAGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGC	3512	0.20024494655193256	No Hit
AGTTTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC	3501	0.19961775566011272	No Hit
AGTTTAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	3374	0.1923765517272837	No Hit
AGTTTAGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC	2934	0.16728891605449034	No Hit
AGTTTAGGGGGGGGCTGGTGAGATGGCTCAGCGGTTAGGAGCGCCGACTG	2650	0.15109598757477827	No Hit
AGTTTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTG	2574	0.14676266868584123	No Hit
AGTTTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT	2355	0.13427586820324633	No Hit
AGTTTAGGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTG	2347	0.13381972937283193	No Hit
AGTTTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGATTG	2343	0.1335916599576247	No Hit
AGTTTAGGGGGGGCTGGCAAGATGGCTCAGCGGGTAAGGGCACTGACTGC	2199	0.12538116101016505	No Hit
AGTTTAGGGGGAGGTTCGAAGACGATCAGATACCGTCGTAGTTCCGACCA	2051	0.1169425926474982	No Hit
AGTTTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG	2031	0.11580224557146213	No Hit
AGTTTAGGGGAGCTGGAGAGATGGCTCAGCGGTTAAGAGCACCAACTGCT	1770	0.10092071622919152	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.02286395887452305	0.0	0.0	0.0	0.0
7	0.02417535801196452	0.0	0.0	0.0	0.0
8	0.033184099912649416	0.0	0.0	0.0	0.0
9	0.05433753817311837	0.0	0.0	0.0	0.0
10	0.09419266848057875	0.0	0.0	0.0	0.0
11	0.11996451239899376	0.0	0.0	0.0	0.0
12	0.131767104635967	0.0	0.0	0.0	0.0
13	0.14203022832029155	0.0	0.0	0.0	0.0
14	0.15417492468007563	0.0	0.0	0.0	0.0
15	0.1701397837445805	0.0	0.0	0.0	0.0
16	0.1846221916102385	0.0	0.0	0.0	0.0
17	0.19864846064548206	0.0	0.0	0.0	0.0
18	0.21318788586494186	0.0	0.0	0.0	0.0
19	0.23759131329211358	0.0	0.0	0.0	0.0
20	0.2523588079267806	0.0	0.0	0.0	0.0
21	0.26632805960822237	0.0	0.0	0.0	0.0
22	0.2800692418744569	0.0	0.0	0.0	0.0
23	0.2943805976787095	0.0	0.0	0.0	0.0
24	0.3097752832051963	0.0	0.0	0.0	0.0
25	0.3238585695942417	0.0	0.0	0.0	0.0
26	0.33862606422890873	0.0	0.0	0.0	0.0
27	0.3505426911734856	0.0	0.0	0.0	0.0
28	0.3698715741122968	0.0	0.0	0.0	0.0
29	0.3844109993317566	0.0	0.0	0.0	0.0
30	0.39946358073543264	0.0	0.0	0.0	0.0
31	0.413261780355469	0.0	0.0	0.0	0.0
32	0.42745910145211796	0.0	0.0	0.0	0.0
33	0.43983186722710926	0.0	0.0	0.0	0.0
34	0.45516953539979427	0.0	0.0	0.0	0.0
35	0.4714194812333082	0.0	0.0	0.0	0.0
36	0.4865860973445878	0.0	0.0	0.0	0.0
37	0.5019807828710746	0.0	0.0	0.0	0.0
38	0.5156079304297055	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACGAA	35	1.4466605E-7	44.0	32
CCGTATA	40	8.3164196E-9	44.0	31
ACGATAA	20	7.8576163E-4	44.0	29
AACGACG	20	7.8576163E-4	44.0	13
TCGTATC	20	7.8576163E-4	44.0	34
TCCGTAT	30	2.5284826E-6	44.0	43
CGATGTA	40	8.3164196E-9	44.0	21
TTATTCG	20	7.8576163E-4	44.0	34
CAATACG	20	7.8576163E-4	44.0	19
CGATAGG	30	2.5284826E-6	44.0	27
TCGGTAC	25	4.443801E-5	44.0	11
TCGAACG	30	2.5284826E-6	44.0	26
TAGGGTA	2255	0.0	42.73171	5
TAGGGAT	23350	0.0	42.690365	5
TAGGGAC	11840	0.0	42.58784	5
TTAGGGA	69775	0.0	42.58431	4
GTTTAGG	179040	0.0	42.556187	2
TTTAGGG	175515	0.0	42.54975	3
AGTTTAG	180595	0.0	42.544254	1
TAGGGGG	32195	0.0	42.469326	5
>>END_MODULE