FastQCFastQC Report
Fri 17 Jun 2016
SRR1527842_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527842_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2207489
Sequences flagged as poor quality0
Sequence length52
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTTTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC203140.9202310860892172No Hit
AGTTTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT118750.5379415254164347No Hit
AGTTTAGGGAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATACCGTCGT84690.3836485708422556No Hit
AGTTTAGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC74490.3374422250801703No Hit
AGTTTAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT56170.2544520040643464No Hit
AGTTTAGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGG53970.24448592948821038No Hit
AGTTTAGGGAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGAAC47910.21703392406485378No Hit
AGTTTAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT44690.2024472149125092No Hit
AGTTTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA42540.19270764203128532No Hit
AGTTTAGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCAT42430.19220933830247852No Hit
AGTTTAGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC37270.16883436338754124No Hit
AGTTTAGGGGGGGGCTGGTGAGATGGCTCAGCGGTTAGGAGCGCCGACTGCT33900.1535681491504601No Hit
AGTTTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT32550.14745260338783114No Hit
AGTTTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC30310.13730532745576535No Hit
AGTTTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGATTGCT29940.1356292149134152No Hit
AGTTTAGGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCT29750.1347685084727489No Hit
AGTTTAGGGGGGGCTGGCAAGATGGCTCAGCGGGTAAGGGCACTGACTGCTC27430.12425882982882361No Hit
AGTTTAGGGGGAGGTTCGAAGACGATCAGATACCGTCGTAGTTCCGACCATA25950.11755437965942299No Hit
AGTTTAGGGGAGCTGGAGAGATGGCTCAGCGGTTAAGAGCACCAACTGCTCT23940.10844901152395324No Hit
AGTTTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGAT23200.10509678643925292No Hit
AGTTTAGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGA22720.10292237016809598No Hit
AGTTTAGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGAGCACTGACTCTTCC22360.10129155796472825No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGTCG206.3127227E-446.00000422
TAACGAC206.3127227E-446.00000416
GTTAACG206.3127227E-446.00000418
CTACGGG206.3127227E-446.00000421
GCGATTA206.3127227E-446.00000445
TTACGCC206.3127227E-446.00000433
TCGGTAC206.3127227E-446.00000411
TATGCGT650.046.044
TCGTAAG301.8621886E-646.037
TAGGGAT299950.044.6734435
TTAGGGA876750.044.4679764
AGTTTAG2274900.044.4612051
GTTTAGG2256200.044.453552
TTTAGGG2212700.044.4283453
TTAGGGG1075650.044.261614
TAGGGAC151200.044.17465
TAGGGGG402900.044.14475
TAGGGGA274050.044.1200525
TAGGGAG268100.043.9410675
TTAGGGC176600.043.8120044
Produced by FastQC (version 0.11.5)