##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527842_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2207489
Sequences flagged as poor quality	0
Sequence length	52
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.474274616996958	31.0	31.0	33.0	30.0	33.0
2	32.24170358266791	33.0	31.0	34.0	31.0	34.0
3	30.765945832572665	31.0	31.0	31.0	28.0	33.0
4	35.38902979810998	35.0	35.0	37.0	35.0	37.0
5	36.18165345331279	37.0	35.0	37.0	35.0	37.0
6	36.03861672696897	37.0	35.0	37.0	35.0	37.0
7	36.319719373460075	37.0	35.0	37.0	35.0	37.0
8	36.59144031974791	37.0	37.0	37.0	35.0	37.0
9	38.70159126500744	39.0	39.0	39.0	38.0	39.0
10	38.1009055990766	39.0	38.0	39.0	37.0	39.0
11	37.83682908499204	39.0	38.0	39.0	35.0	39.0
12	37.42546576676033	39.0	37.0	39.0	35.0	39.0
13	37.32649494516168	39.0	37.0	39.0	35.0	39.0
14	38.10960598218156	40.0	38.0	40.0	35.0	40.0
15	38.120953264093274	40.0	38.0	40.0	34.0	40.0
16	38.15820645085887	40.0	38.0	40.0	35.0	40.0
17	38.05466074802638	40.0	38.0	40.0	34.0	40.0
18	38.027297078264034	40.0	38.0	40.0	34.0	40.0
19	38.03741128494865	40.0	38.0	40.0	34.0	40.0
20	38.023647229952225	40.0	38.0	40.0	34.0	40.0
21	37.98490230302394	40.0	38.0	40.0	34.0	40.0
22	37.99208738978994	40.0	38.0	40.0	34.0	40.0
23	37.9888570226171	40.0	38.0	40.0	34.0	40.0
24	37.95003463210915	40.0	37.0	40.0	34.0	40.0
25	37.93639515304493	40.0	37.0	40.0	34.0	40.0
26	37.83720281278865	40.0	37.0	40.0	34.0	40.0
27	37.76505069787437	40.0	37.0	40.0	34.0	40.0
28	37.66335777890626	40.0	37.0	40.0	34.0	40.0
29	37.60630336096805	40.0	36.0	40.0	34.0	40.0
30	37.51211806717949	40.0	36.0	40.0	33.0	40.0
31	37.42929364540435	40.0	36.0	40.0	33.0	40.0
32	37.33985537413776	40.0	36.0	40.0	33.0	40.0
33	37.21803823258009	39.0	35.0	40.0	33.0	40.0
34	37.00018935541695	39.0	35.0	40.0	32.0	40.0
35	36.840696827934366	39.0	35.0	40.0	31.0	40.0
36	36.9515372443532	39.0	35.0	40.0	32.0	40.0
37	36.9084878792148	39.0	35.0	40.0	32.0	40.0
38	36.85683643270703	39.0	35.0	40.0	32.0	40.0
39	36.78865036247066	39.0	35.0	40.0	32.0	40.0
40	36.58184163092093	39.0	35.0	40.0	31.0	40.0
41	36.52717046381658	39.0	35.0	40.0	31.0	40.0
42	36.43301280323481	38.0	35.0	40.0	31.0	40.0
43	36.303415781460295	38.0	35.0	40.0	31.0	40.0
44	36.23558124185443	38.0	35.0	40.0	31.0	40.0
45	36.115795820500125	38.0	35.0	40.0	30.0	40.0
46	35.97055251464447	38.0	35.0	40.0	30.0	40.0
47	35.84133057967673	38.0	35.0	40.0	30.0	40.0
48	35.74090652320351	38.0	34.0	40.0	30.0	40.0
49	36.06336566116524	38.0	35.0	40.0	31.0	40.0
50	36.122741268472915	38.0	35.0	40.0	31.0	40.0
51	36.132052300147365	38.0	35.0	40.0	31.0	40.0
52	35.694894062892274	37.0	35.0	40.0	30.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	0.0
14	1.0
15	9.0
16	32.0
17	69.0
18	156.0
19	357.0
20	688.0
21	1256.0
22	1982.0
23	3111.0
24	4663.0
25	6703.0
26	9427.0
27	12978.0
28	17359.0
29	23168.0
30	31115.0
31	40924.0
32	55709.0
33	80489.0
34	166986.0
35	148933.0
36	201904.0
37	334870.0
38	845336.0
39	219263.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	100.0	0.0	0.0	0.0
3	0.0	0.0	100.0	0.0
4	0.0	0.0	100.0	0.0
5	0.0	0.0	100.0	0.0
6	0.0	100.0	0.0	0.0
7	99.67030413288582	0.29794032948748556	0.023239073897989976	0.008516463728698083
8	99.11831950238484	0.8031297098196185	0.059569945761904136	0.018980842033640935
9	97.8350061993514	1.0786010711718155	0.3704661721983666	0.7159265572784281
10	48.300625733582365	39.60644877505619	4.220360780959724	7.872564710401727
11	33.78961344767743	23.24396633460008	24.897202205764106	18.069218011958384
12	31.05668929720601	18.502425153647426	26.28515929184698	24.155726257299584
13	26.943146715566872	18.324123019412557	28.362632837581526	26.37009742743905
14	25.313784123046595	20.41722518209604	29.724043925020695	24.544946769836677
15	27.047337495226476	19.354841632279935	28.585872908086973	25.011947964406616
16	31.01075475347782	21.703165904790467	25.58848537863609	21.697593963095628
17	29.440780905363518	22.37723494884912	24.29529660170447	23.886687544082893
18	28.568205775883822	22.15911381664869	25.403750596265713	23.868929811201777
19	27.635788898608325	24.820916434917685	24.501367843735576	23.041926822738414
20	28.729792085034173	24.407641442380914	23.60813575967989	23.254430712905023
21	27.776854154199636	24.57547919831084	26.168103215916368	21.47956343157316
22	27.093000236920773	22.01537584105742	27.701202588098965	23.190421333922842
23	27.866050521656057	22.35549078613755	26.972863737939353	22.805594954267043
24	28.27180565792174	22.18629402003815	25.656073484397883	23.885826837642227
25	28.283176043006332	22.918211596977383	24.385987880347308	24.41262447966898
26	25.903639836936897	23.076626882398962	25.096297195591916	25.923436085072225
27	23.88963206611675	21.71163706818018	24.79283022474857	29.605900640954495
28	25.378835409825374	24.864540661357772	24.091716878317403	25.66490705049946
29	28.09350352368687	23.43409185730937	23.74589409052548	24.726510528478286
30	26.796464217941747	22.40341854478097	25.21842690948856	25.581690327788724
31	29.702616864681996	22.800204213928133	24.185805682383922	23.31137323900595
32	30.651115362296256	23.650310375272536	23.407636459343625	22.29093780308758
33	30.649439249753907	22.18701882546187	24.72678233051218	22.436759594272043
34	28.00507726199315	23.604466432222313	26.939296186753364	21.451160119031172
35	25.458111003044635	26.924890678956952	26.741379005739102	20.875619312259314
36	25.18282084304837	28.0227443941963	24.575343297293895	22.219091465461435
37	28.17237141385529	27.27796152098606	22.60251353460878	21.94715353054987
38	27.616173851828936	27.912755171146948	23.451713689173538	21.019357287850585
39	28.516065085715038	24.63976037932692	23.003240333247412	23.84093420171063
40	26.47877294065791	24.416746810516383	23.652303590187763	25.45217665863794
41	24.5717192701753	26.27609922405049	23.42666260171625	25.72551890405796
42	24.147073892553937	24.18961091085845	23.893346693913312	27.76996850267431
43	23.98942871289506	23.84315391832077	24.935073289153422	27.23234407963075
44	24.45348538543114	23.02883502477249	25.745133950837356	26.772545638959016
45	26.343007824727554	23.5756101162905	24.939603323051664	25.14177873593028
46	26.232792099983282	25.31536963491098	23.29135048917571	25.16048777593003
47	23.011983298671023	25.84316388439535	25.013850578643883	26.131002238289746
48	25.586175061347983	23.87364104645595	25.24673962135259	25.29344427084348
49	26.759680342688004	24.03314354001311	25.102865744744367	24.10431037255452
50	24.178059324417926	24.38340576102531	25.21792860575976	26.220606308797006
51	23.687230151543222	23.605100636968064	26.03174919557923	26.675920015909476
52	23.84632494204954	23.772032386118344	26.668989064045167	25.712653607786944
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	5.0
2	10.0
3	12.0
4	14.0
5	50.0
6	86.0
7	758.0
8	1430.0
9	2843.0
10	4256.0
11	5252.0
12	6248.0
13	5746.5
14	5264.5
15	5284.0
16	5309.0
17	5334.0
18	5594.5
19	5855.0
20	7126.0
21	8397.0
22	9671.5
23	10946.0
24	14485.5
25	18025.0
26	19380.5
27	20736.0
28	24266.5
29	27797.0
30	28981.0
31	30165.0
32	35798.5
33	41432.0
34	45569.5
35	49707.0
36	57534.5
37	65362.0
38	66158.0
39	75623.5
40	84293.0
41	87756.5
42	91220.0
43	96861.5
44	102503.0
45	109987.0
46	117471.0
47	122722.0
48	127973.0
49	135955.5
50	143938.0
51	144148.0
52	144358.0
53	158700.5
54	173043.0
55	172536.5
56	172030.0
57	169495.0
58	166960.0
59	161892.0
60	156824.0
61	132636.0
62	108448.0
63	97997.5
64	68150.0
65	48753.0
66	40389.5
67	32026.0
68	29797.5
69	27569.0
70	27452.5
71	27336.0
72	20447.5
73	13559.0
74	9437.5
75	5316.0
76	3676.0
77	2036.0
78	1415.5
79	795.0
80	476.0
81	157.0
82	96.5
83	36.0
84	24.0
85	12.0
86	6.5
87	1.0
88	1.5
89	1.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	2207489.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	7.448215744895653
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.3436496086366	4.941418155888874
2	9.685873065937352	1.4428494454555063
3	4.31245454023172	0.963602754171019
4	2.169686659839038	0.6464117736521273
5	1.245564415207927	0.46386162443167134
6	0.7487118834403191	0.3345940583178399
7	0.5156133232836598	0.2688279490931529
8	0.38888488399149773	0.2317198812717914
9	0.3040314094452272	0.20380423776954823
>10	6.25690323179703	13.716451158302537
>50	5.044201765657571	26.78596773418858
>100	2.839644698756575	36.690490971030435
>500	0.10158968823743532	5.13080211409921
>1k	0.03954089661936105	5.500479034347669
>5k	0.002433285945806834	1.220264593226068
>10k+	0.001216642972903417	1.4584545147539694
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AGTTTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	20314	0.9202310860892172	No Hit
AGTTTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	11875	0.5379415254164347	No Hit
AGTTTAGGGAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATACCGTCGT	8469	0.3836485708422556	No Hit
AGTTTAGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC	7449	0.3374422250801703	No Hit
AGTTTAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	5617	0.2544520040643464	No Hit
AGTTTAGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGG	5397	0.24448592948821038	No Hit
AGTTTAGGGAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGAAC	4791	0.21703392406485378	No Hit
AGTTTAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT	4469	0.2024472149125092	No Hit
AGTTTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	4254	0.19270764203128532	No Hit
AGTTTAGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCAT	4243	0.19220933830247852	No Hit
AGTTTAGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	3727	0.16883436338754124	No Hit
AGTTTAGGGGGGGGCTGGTGAGATGGCTCAGCGGTTAGGAGCGCCGACTGCT	3390	0.1535681491504601	No Hit
AGTTTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT	3255	0.14745260338783114	No Hit
AGTTTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	3031	0.13730532745576535	No Hit
AGTTTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGATTGCT	2994	0.1356292149134152	No Hit
AGTTTAGGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCT	2975	0.1347685084727489	No Hit
AGTTTAGGGGGGGCTGGCAAGATGGCTCAGCGGGTAAGGGCACTGACTGCTC	2743	0.12425882982882361	No Hit
AGTTTAGGGGGAGGTTCGAAGACGATCAGATACCGTCGTAGTTCCGACCATA	2595	0.11755437965942299	No Hit
AGTTTAGGGGAGCTGGAGAGATGGCTCAGCGGTTAAGAGCACCAACTGCTCT	2394	0.10844901152395324	No Hit
AGTTTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGAT	2320	0.10509678643925292	No Hit
AGTTTAGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGA	2272	0.10292237016809598	No Hit
AGTTTAGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGAGCACTGACTCTTCC	2236	0.10129155796472825	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.01907144271160581	0.0	0.0	0.0	0.0
7	0.020385152542096472	0.0	0.0	0.0	0.0
8	0.03148373559279344	0.0	0.0	0.0	0.0
9	0.051687686778960164	0.0	0.0	0.0	0.0
10	0.0876108555920324	0.0	0.0	0.0	0.0
11	0.11048752677816288	0.0	0.0	0.0	0.0
12	0.12185791186275446	0.0	0.0	0.0	0.0
13	0.13155218440499591	0.0	0.0	0.0	0.0
14	0.1431943715234821	0.0	0.0	0.0	0.0
15	0.1567844732182131	0.0	0.0	0.0	0.0
16	0.1702839742349792	0.0	0.0	0.0	0.0
17	0.18188086101448298	0.0	0.0	0.0	0.0
18	0.19338714711602187	0.0	0.0	0.0	0.0
19	0.2155843132174158	0.0	0.0	0.0	0.0
20	0.22890261287825217	0.0	0.0	0.0	0.0
21	0.2418132094882466	0.0	0.0	0.0	0.0
22	0.2525946901660665	0.0	0.0	0.0	0.0
23	0.2650069830472541	0.0	0.0	0.0	0.0
24	0.27850648406402023	0.0	0.0	0.0	0.0
25	0.2916888827079093	0.0	0.0	0.0	0.0
26	0.3056866874534822	0.0	0.0	0.0	0.0
27	0.31587926372453046	0.0	0.0	0.0	0.0
28	0.33159848135143594	0.0	0.0	0.0	0.0
29	0.3436030711817816	0.0	0.0	0.0	0.0
30	0.35619656541889905	0.0	0.0	0.0	0.0
31	0.36856355796110424	0.0	0.0	0.0	0.0
32	0.38197245829990545	0.0	0.0	0.0	0.0
33	0.3935240447404268	0.0	0.0	0.0	0.0
34	0.40756714982498216	0.0	0.0	0.0	0.0
35	0.42301456541799304	0.0	0.0	0.0	0.0
36	0.43705767050254835	0.0	0.0	0.0	0.0
37	0.4514178779599808	0.0	0.0	0.0	0.0
38	0.46405667253608057	0.0	0.0	0.0	0.0
39	0.47474755253593565	0.0	0.0	0.0	0.0
40	0.4862538386374745	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAAGTCG	20	6.3127227E-4	46.000004	22
TAACGAC	20	6.3127227E-4	46.000004	16
GTTAACG	20	6.3127227E-4	46.000004	18
CTACGGG	20	6.3127227E-4	46.000004	21
GCGATTA	20	6.3127227E-4	46.000004	45
TTACGCC	20	6.3127227E-4	46.000004	33
TCGGTAC	20	6.3127227E-4	46.000004	11
TATGCGT	65	0.0	46.0	44
TCGTAAG	30	1.8621886E-6	46.0	37
TAGGGAT	29995	0.0	44.673443	5
TTAGGGA	87675	0.0	44.467976	4
AGTTTAG	227490	0.0	44.461205	1
GTTTAGG	225620	0.0	44.45355	2
TTTAGGG	221270	0.0	44.428345	3
TTAGGGG	107565	0.0	44.26161	4
TAGGGAC	15120	0.0	44.1746	5
TAGGGGG	40290	0.0	44.1447	5
TAGGGGA	27405	0.0	44.120052	5
TAGGGAG	26810	0.0	43.941067	5
TTAGGGC	17660	0.0	43.812004	4
>>END_MODULE