FastQCFastQC Report
Fri 17 Jun 2016
SRR1527841_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527841_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2220234
Sequences flagged as poor quality0
Sequence length52
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTTTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC189840.855045008769346No Hit
AGTTTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT112650.5073789519483081No Hit
AGTTTAGGGAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATACCGTCGT78070.3516296030058093No Hit
AGTTTAGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC70500.31753409775726343No Hit
AGTTTAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT53280.23997470536889354No Hit
AGTTTAGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGG51320.23114680704826607No Hit
AGTTTAGGGAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGAAC45030.20281645988666058No Hit
AGTTTAGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCAT41680.1877279602059963No Hit
AGTTTAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT41420.18655691246958656No Hit
AGTTTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA41150.18534082443562255No Hit
AGTTTAGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC34550.15561422804983618No Hit
AGTTTAGGGGGGGGCTGGTGAGATGGCTCAGCGGTTAGGAGCGCCGACTGCT31980.1440388715784012No Hit
AGTTTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT30700.1382737134914608No Hit
AGTTTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC30460.1371927463501595No Hit
AGTTTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGATTGCT29610.13336432105805063No Hit
AGTTTAGGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCT28360.12773428386377292No Hit
AGTTTAGGGGGAGGTTCGAAGACGATCAGATACCGTCGTAGTTCCGACCATA27070.12192408547927831No Hit
AGTTTAGGGGGGGCTGGCAAGATGGCTCAGCGGGTAAGGGCACTGACTGCTC26410.11895142584069968No Hit
AGTTTAGGGGAGCTGGAGAGATGGCTCAGCGGTTAAGAGCACCAACTGCTCT22680.10215139485297495No Hit
AGTTTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGAT22430.10102538741411941No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGTA453.110472E-1046.09
ACGCGTA405.6152203E-946.013
TCACGTA351.0201438E-745.99999636
TAGGGTA29100.044.4982835
AGTTTAG2288400.044.45321
TAGGGAC150850.044.4448175
GTTTAGG2271550.044.4396972
TAGGGGA264600.044.4353755
TTAGGGA879900.044.429034
TTTAGGG2224950.044.420463
TAGGGAT302350.044.3644755
TAGGGAG263000.044.3121645
TTAGGGG1083900.044.2981834
TTAGGGT92100.044.027144
TAGGGGG413000.043.9561735
TAGGGGC226100.043.924815
TAGGGGT186150.043.8995445
TTAGGGC176200.043.5329174
AGGGATT108400.043.432666
TAGGGTG38100.043.2230955
Produced by FastQC (version 0.11.5)