##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527841_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2220234
Sequences flagged as poor quality	0
Sequence length	52
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.45646089556326	31.0	31.0	33.0	30.0	33.0
2	32.23403298931554	33.0	31.0	34.0	31.0	34.0
3	30.7553073234623	31.0	31.0	31.0	28.0	33.0
4	35.371383376707136	35.0	35.0	37.0	35.0	37.0
5	36.17433297571337	37.0	35.0	37.0	35.0	37.0
6	35.952269445472865	37.0	35.0	37.0	35.0	37.0
7	36.25664727231454	37.0	35.0	37.0	35.0	37.0
8	36.55401187442405	37.0	37.0	37.0	35.0	37.0
9	38.651664644357304	39.0	39.0	39.0	38.0	39.0
10	38.010352512392835	39.0	38.0	39.0	37.0	39.0
11	37.79829918828376	39.0	38.0	39.0	35.0	39.0
12	37.37413128526092	39.0	37.0	39.0	35.0	39.0
13	37.124262577728295	39.0	37.0	39.0	34.0	39.0
14	38.0404331255174	40.0	38.0	40.0	34.0	40.0
15	38.0688850814824	40.0	38.0	40.0	34.0	40.0
16	38.13997533593306	40.0	38.0	40.0	35.0	40.0
17	38.07057229102878	40.0	38.0	40.0	34.0	40.0
18	38.05360335892523	40.0	38.0	40.0	34.0	40.0
19	38.019189418772974	40.0	38.0	40.0	34.0	40.0
20	38.01795891784379	40.0	38.0	40.0	34.0	40.0
21	38.031486771214205	40.0	38.0	40.0	34.0	40.0
22	37.93009430537502	40.0	38.0	40.0	34.0	40.0
23	37.95326798886964	40.0	37.0	40.0	34.0	40.0
24	37.96054695135738	40.0	37.0	40.0	34.0	40.0
25	37.92070655615579	40.0	37.0	40.0	34.0	40.0
26	37.77642987180631	40.0	37.0	40.0	34.0	40.0
27	37.701723782268	40.0	37.0	40.0	34.0	40.0
28	37.62577998535289	40.0	37.0	40.0	34.0	40.0
29	37.56472020516756	40.0	36.0	40.0	34.0	40.0
30	37.4524050167685	40.0	36.0	40.0	33.0	40.0
31	37.393300886302974	40.0	36.0	40.0	33.0	40.0
32	37.30531196261295	39.0	36.0	40.0	33.0	40.0
33	37.0065817386816	39.0	35.0	40.0	32.0	40.0
34	36.89949482802263	39.0	35.0	40.0	31.0	40.0
35	36.817446719580005	39.0	35.0	40.0	31.0	40.0
36	36.78412635785237	39.0	35.0	40.0	31.0	40.0
37	36.81758814611433	39.0	35.0	40.0	32.0	40.0
38	36.49145855797182	39.0	35.0	40.0	31.0	40.0
39	36.59843331828987	39.0	35.0	40.0	31.0	40.0
40	36.47655787633195	39.0	35.0	40.0	31.0	40.0
41	36.21649384704495	38.0	35.0	40.0	31.0	40.0
42	36.26906127912643	38.0	35.0	40.0	31.0	40.0
43	36.25244726456761	38.0	35.0	40.0	31.0	40.0
44	36.181196666657655	38.0	35.0	40.0	31.0	40.0
45	36.05758447082605	38.0	35.0	40.0	30.0	40.0
46	35.740289987451774	38.0	35.0	40.0	30.0	40.0
47	35.521768876613905	38.0	34.0	40.0	29.0	40.0
48	35.59213938710965	38.0	34.0	40.0	29.0	40.0
49	35.54828815341086	38.0	34.0	40.0	29.0	40.0
50	35.390112483639115	37.0	34.0	40.0	29.0	40.0
51	35.74242219513799	38.0	35.0	40.0	30.0	40.0
52	35.50723482299613	37.0	34.0	40.0	30.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	2.0
14	3.0
15	16.0
16	30.0
17	81.0
18	170.0
19	410.0
20	704.0
21	1263.0
22	2028.0
23	3249.0
24	4995.0
25	7101.0
26	10040.0
27	13529.0
28	18260.0
29	24591.0
30	33065.0
31	43715.0
32	59054.0
33	87415.0
34	170895.0
35	153595.0
36	216433.0
37	364577.0
38	822066.0
39	182947.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	100.0	0.0	0.0	0.0
3	0.0	0.0	100.0	0.0
4	0.0	0.0	100.0	0.0
5	0.0	0.0	100.0	0.0
6	0.0	100.0	0.0	0.0
7	99.66413450113816	0.29474370719482723	0.030582362039316576	0.010539429627687892
8	99.12175923799023	0.7959070980806529	0.061840328541946474	0.0204933353871709
9	97.81225762689878	1.0719140414929238	0.3941026035994404	0.7217257280088495
10	48.25712064584183	39.61217601387962	4.257118844229932	7.873584496048615
11	33.71991420724122	23.234262694833067	24.929039011203326	18.11678408672239
12	31.035512473009604	18.487465735593638	26.291327851028317	24.185693940368445
13	27.171685507023135	18.34284134014703	28.298098308556668	26.18737484427317
14	25.284542079798793	20.40870466806652	29.749026453968362	24.557726798166318
15	26.969139288921802	19.35998637981402	28.624550385229664	25.046323946034516
16	30.990201933670054	21.763877140877945	25.56865627677083	21.677264648681174
17	29.369561947074047	22.398810215499807	24.328967126888426	23.902660710537717
18	28.5742403728616	22.116993073703043	25.390296698456112	23.91846985497925
19	27.61461179317135	24.908590716113707	24.4369287201259	23.039868770589045
20	28.66517673362357	24.368017064867935	23.72087806960888	23.24592813189961
21	27.732617372763414	24.61952208641071	26.163953889544977	21.4839066512809
22	27.233931198243067	21.99538427030664	27.683388327536644	23.087296203913642
23	27.76139812290056	22.377596235351767	27.008909871662173	22.852095770085494
24	28.276208723945313	22.170591027792565	25.69296749802048	23.860232750241643
25	28.288504725177617	22.896505503473957	24.424047195025388	24.390942576323035
26	25.997935352760116	23.03608538559449	25.13433268745547	25.831646574189925
27	23.845234331156085	21.794729744702586	24.742887461411726	29.617148462729602
28	25.347823697862477	24.828238825276973	24.07435432481441	25.749583152046135
29	28.064789567225795	23.464463655632695	23.749748900341135	24.720997876800375
30	26.789293380787793	22.431734673011945	25.25341923418883	25.525552712011436
31	29.706238171291854	22.802281200990528	24.18015398376928	23.31132664394834
32	30.58632558550135	23.70871718926924	23.3994254659644	22.305531759265016
33	30.619880607179244	22.247294654527405	24.783288608317864	22.34953612997549
34	27.990968519534427	23.62223981796513	26.882932159403016	21.503859503097424
35	25.505374658707147	26.88820187421686	26.762854726123464	20.84356874095253
36	25.423761639538895	27.887465915754827	24.592587988473287	22.096184456232994
37	28.091588544270557	27.30000531475511	22.607346793175854	22.001059347798474
38	27.645374316400883	27.819590187340616	23.386904263244325	21.148131233014176
39	28.477313652524916	24.643033121734014	23.017528783002152	23.862124442738917
40	26.44572599104419	24.364684082848925	23.690205626974453	25.499384299132434
41	24.813015204703646	26.194220969501416	23.457257208024018	25.53550661777092
42	24.158399520050587	24.157363593206842	23.903381355298585	27.780855531443983
43	23.961213097358204	23.819065918277083	24.95885568818422	27.26086529618049
44	24.52854068535118	23.062929402936806	25.72264905410871	26.6858808576033
45	26.348664149814837	23.498243878798363	24.946559686951915	25.206532284434886
46	26.4223951169111	25.221665824413105	23.31965909899587	25.036279959679923
47	23.338440903075984	25.808856183627494	24.96218867020323	25.890514243093293
48	25.613336251944613	23.88730196907173	25.210991273892752	25.288370505090906
49	26.83356799328359	23.998731665220873	25.007769451328105	24.159930890167434
50	24.179568459901073	24.310770846676522	25.292829494548773	26.21683119887363
51	23.667910679685114	23.58382044415138	26.04964161435236	26.698627261811144
52	23.848522272877542	23.752676519682158	26.705788669122267	25.693012538318033
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	3.0
2	6.0
3	11.5
4	17.0
5	44.0
6	71.0
7	735.0
8	1399.0
9	2803.5
10	4208.0
11	5188.0
12	6168.0
13	5800.0
14	5312.5
15	5193.0
16	5328.0
17	5463.0
18	5699.5
19	5936.0
20	7231.0
21	8526.0
22	9567.5
23	10609.0
24	14216.0
25	17823.0
26	19404.5
27	20986.0
28	24554.5
29	28123.0
30	29258.0
31	30393.0
32	35861.0
33	41329.0
34	45745.0
35	50161.0
36	58000.0
37	65839.0
38	66667.5
39	76222.0
40	84948.0
41	88689.5
42	92431.0
43	97539.5
44	102648.0
45	110486.5
46	118325.0
47	123431.5
48	128538.0
49	136770.0
50	145002.0
51	145269.0
52	145536.0
53	159258.5
54	172981.0
55	173127.5
56	173274.0
57	170650.5
58	168027.0
59	162652.0
60	157277.0
61	133432.0
62	109587.0
63	98375.5
64	68307.5
65	49451.0
66	41323.5
67	33196.0
68	30410.5
69	27625.0
70	27395.0
71	27165.0
72	20366.5
73	13568.0
74	9390.5
75	5213.0
76	3670.5
77	2128.0
78	1449.5
79	771.0
80	466.5
81	162.0
82	93.5
83	25.0
84	17.0
85	9.0
86	6.0
87	3.0
88	2.0
89	1.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	2220234.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	7.885349646277836
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.39190417390715	5.235233780934308
2	9.903040250236353	1.561778698685528
3	4.296472048611922	1.0163755304629387
4	2.192173856891852	0.6914422938808675
5	1.1842190839032967	0.46689907671861625
6	0.8119356243266447	0.3841437772850689
7	0.49707050119135776	0.2743702290521094
8	0.38334187594063446	0.24182277806815716
9	0.28874025738079306	0.2049136097763427
>10	6.482832680597585	15.013502655680433
>50	4.885981441288502	27.2650940380839
>100	2.556499790538099	35.16626607741985
>500	0.0862549132702639	4.627288186114877
>1k	0.03593954719594329	5.226339877121073
>5k	0.002395969813062886	1.1957889821969454
>10k+	0.001197984906531443	1.4287404085189952
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AGTTTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	18984	0.855045008769346	No Hit
AGTTTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	11265	0.5073789519483081	No Hit
AGTTTAGGGAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATACCGTCGT	7807	0.3516296030058093	No Hit
AGTTTAGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC	7050	0.31753409775726343	No Hit
AGTTTAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	5328	0.23997470536889354	No Hit
AGTTTAGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGG	5132	0.23114680704826607	No Hit
AGTTTAGGGAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGAAC	4503	0.20281645988666058	No Hit
AGTTTAGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCAT	4168	0.1877279602059963	No Hit
AGTTTAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT	4142	0.18655691246958656	No Hit
AGTTTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	4115	0.18534082443562255	No Hit
AGTTTAGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	3455	0.15561422804983618	No Hit
AGTTTAGGGGGGGGCTGGTGAGATGGCTCAGCGGTTAGGAGCGCCGACTGCT	3198	0.1440388715784012	No Hit
AGTTTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT	3070	0.1382737134914608	No Hit
AGTTTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	3046	0.1371927463501595	No Hit
AGTTTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGATTGCT	2961	0.13336432105805063	No Hit
AGTTTAGGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCT	2836	0.12773428386377292	No Hit
AGTTTAGGGGGAGGTTCGAAGACGATCAGATACCGTCGTAGTTCCGACCATA	2707	0.12192408547927831	No Hit
AGTTTAGGGGGGGCTGGCAAGATGGCTCAGCGGGTAAGGGCACTGACTGCTC	2641	0.11895142584069968	No Hit
AGTTTAGGGGAGCTGGAGAGATGGCTCAGCGGTTAAGAGCACCAACTGCTCT	2268	0.10215139485297495	No Hit
AGTTTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGAT	2243	0.10102538741411941	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.01941236824586958	0.0	0.0	0.0	0.0
7	0.020583415982279345	0.0	0.0	0.0	0.0
8	0.029276193410244147	0.0	0.0	0.0	0.0
9	0.04832823927567995	0.0	0.0	0.0	0.0
10	0.08228862363156315	0.0	0.0	0.0	0.0
11	0.10566453806220426	0.0	0.0	0.0	0.0
12	0.1185911034602659	0.0	0.0	0.0	0.0
13	0.12674339731758003	0.0	0.0	0.0	0.0
14	0.1397150030131959	0.0	0.0	0.0	0.0
15	0.15394773704033	0.0	0.0	0.0	0.0
16	0.1686759143405605	0.0	0.0	0.0	0.0
17	0.18092687527530882	0.0	0.0	0.0	0.0
18	0.1927274332345149	0.0	0.0	0.0	0.0
19	0.21556286409450534	0.0	0.0	0.0	0.0
20	0.2285344697901212	0.0	0.0	0.0	0.0
21	0.24263208292459262	0.0	0.0	0.0	0.0
22	0.2530363916596179	0.0	0.0	0.0	0.0
23	0.26569271527235416	0.0	0.0	0.0	0.0
24	0.2793399254312834	0.0	0.0	0.0	0.0
25	0.29046487892717615	0.0	0.0	0.0	4.504029755422176E-5
26	0.30330136373012934	0.0	0.0	0.0	4.504029755422176E-5
27	0.3155973649624319	0.0	0.0	0.0	4.504029755422176E-5
28	0.3309110661308673	0.0	0.0	0.0	4.504029755422176E-5
29	0.34226122111453117	0.0	0.0	0.0	4.504029755422176E-5
30	0.3532510537177613	0.0	0.0	0.0	4.504029755422176E-5
31	0.36306983858458164	0.0	0.0	0.0	4.504029755422176E-5
32	0.3758612830899806	0.0	0.0	0.0	4.504029755422176E-5
33	0.38734655896630715	0.0	0.0	0.0	4.504029755422176E-5
34	0.40036320495947725	0.0	0.0	0.0	4.504029755422176E-5
35	0.41504634196215356	0.0	0.0	0.0	4.504029755422176E-5
36	0.42918899539417915	0.0	0.0	0.0	4.504029755422176E-5
37	0.4423407622800119	0.0	0.0	0.0	4.504029755422176E-5
38	0.4563032545218207	0.0	0.0	0.0	4.504029755422176E-5
39	0.4678786109932557	0.0	0.0	0.0	4.504029755422176E-5
40	0.4791386853818111	0.0	0.0	0.0	4.504029755422176E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAACGTA	45	3.110472E-10	46.0	9
ACGCGTA	40	5.6152203E-9	46.0	13
TCACGTA	35	1.0201438E-7	45.999996	36
TAGGGTA	2910	0.0	44.498283	5
AGTTTAG	228840	0.0	44.4532	1
TAGGGAC	15085	0.0	44.444817	5
GTTTAGG	227155	0.0	44.439697	2
TAGGGGA	26460	0.0	44.435375	5
TTAGGGA	87990	0.0	44.42903	4
TTTAGGG	222495	0.0	44.42046	3
TAGGGAT	30235	0.0	44.364475	5
TAGGGAG	26300	0.0	44.312164	5
TTAGGGG	108390	0.0	44.298183	4
TTAGGGT	9210	0.0	44.02714	4
TAGGGGG	41300	0.0	43.956173	5
TAGGGGC	22610	0.0	43.92481	5
TAGGGGT	18615	0.0	43.899544	5
TTAGGGC	17620	0.0	43.532917	4
AGGGATT	10840	0.0	43.43266	6
TAGGGTG	3810	0.0	43.223095	5
>>END_MODULE