FastQCFastQC Report
Fri 17 Jun 2016
SRR1527840_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527840_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1210368
Sequences flagged as poor quality0
Sequence length50
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC251172.075154002749577No Hit
ATTTGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG154741.2784541560913707No Hit
ATTTGCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT72880.6021309221658206No Hit
ATTTGCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC62850.5192635628172588No Hit
ATTTGCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT54310.4487065090947546No Hit
ATTTGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGT49140.40599222715736044No Hit
ATTTGCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG45740.37790159686971236No Hit
ATTTGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT42130.34807595706429784No Hit
ATTTGCGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC37120.3066835871404399No Hit
ATTTGCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC36710.303296187605753No Hit
ATTTGCGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG30580.2526504335871404No Hit
ATTTGCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA30380.25099804357022No Hit
ATTTGCGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCG29960.24752802453468697No Hit
ATTTGCGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCT26800.22142026226734346No Hit
ATTTGCGGGGGAGTGGCCTTGTTCTCGATACTTCGTTGTGGTTGTGAACT25310.20910995664128595No Hit
ATTTGCGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCT24920.20588779610829105No Hit
ATTTGCGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCG23950.19787370452622674No Hit
ATTTGCGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTA23600.1949820219966159No Hit
ATTTGCGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTC23020.19019009094754655No Hit
ATTTGCGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGA20750.17143546425549916No Hit
ATTTGCGGGGGGGCTGGAGCGATGGCTGAGTGGTTAAAAGCACCGACTGC19660.16242993866328256No Hit
ATTTGCGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG19140.15813372461928935No Hit
ATTTGCGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAA18720.15466370558375636No Hit
ATTTGCGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAA18690.15441584708121828No Hit
ATTTGCGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGC18310.15127630604906936No Hit
ATTTGCGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGC18250.15078058904399325No Hit
ATTTGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTG17940.1482193845177665No Hit
ATTTGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGC17570.14516246298646363No Hit
ATTTGCGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC17270.14268387796108292No Hit
ATTTGCGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA16590.1370657519035533No Hit
ATTTGCGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGC16580.13698313240270726No Hit
ATTTGCGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTC16470.13607431789340102No Hit
ATTTGCGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG15740.1300430943316413No Hit
ATTTGCGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTC15570.12863856281725888No Hit
ATTTGCGGGGTCGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGAC15550.12847332381556684No Hit
ATTTGCGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGC15080.12459020727580371No Hit
ATTTGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGC14800.12227686125211505No Hit
ATTTGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACT14600.12062447123519458No Hit
ATTTGCGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCA14400.11897208121827413No Hit
ATTTGCGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCC14280.11798064720812183No Hit
ATTTGCGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTC14120.11665873519458546No Hit
ATTTGCGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGT13440.11104060913705585No Hit
ATTTGCGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGT13180.10889250211505923No Hit
ATTTGCGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCC12920.1067443950930626No Hit
ATTTGCGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACTGACTGCT12870.10633129758883249No Hit
ATTTGCGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGC12820.10591820008460237No Hit
ATTTGCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC12720.10509200507614212No Hit
ATTTGCGGGGCTCTCAGCTCCTGAAGGCAAATCCCAATTCCAGAGCAAGG12560.10377009306260575No Hit
ATTTGCGGGGGCTCCGCTTCAAGGGAGGCTCTCAGCGATACGTGCCCGGC12180.10063055203045686No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGATA850.044.019
GTCATAT254.4429806E-544.035
TTAGGAT207.8566483E-444.032
GCCTTAA254.4429806E-544.030
ACACGTA302.5278277E-644.011
CTCGTTA254.4429806E-544.044
CATATCT254.4429806E-544.027
CTCGTAA254.4429806E-544.017
GTCGAAC207.8566483E-444.022
ACGTTTA207.8566483E-444.010
ACGTTAA302.5278277E-644.011
CGACCAA207.8566483E-444.037
TATTGCG207.8566483E-444.038
CGTTAAC207.8566483E-444.012
TTATAAC254.4429806E-544.021
AAACGAA207.8566483E-444.027
AGACGAA207.8566483E-444.025
ACTAGAT850.044.018
AATGACG207.8566483E-444.044
CGGATAT351.4461693E-744.044
Produced by FastQC (version 0.11.5)