FastQCFastQC Report
Fri 17 Jun 2016
SRR1527839_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527839_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1558990
Sequences flagged as poor quality0
Sequence length52
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC304781.9549836753282572No Hit
ATTTGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT193231.239456314665264No Hit
ATTTGCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG99510.6382978723404255No Hit
ATTTGCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT80940.5191822910987242No Hit
ATTTGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC65580.4206569638034881No Hit
ATTTGCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT63410.4067376955593044No Hit
ATTTGCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG62870.4032739145215813No Hit
ATTTGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC56720.36382529714751216No Hit
ATTTGCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC48540.3113554288353357No Hit
ATTTGCGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC45620.29262535359431424No Hit
ATTTGCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC40800.26170790062797067No Hit
ATTTGCGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA38770.24868664968986331No Hit
ATTTGCGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT38530.24714719145087524No Hit
ATTTGCGGGGGAGTGGCCTTGTTCTCGATACTTCGTTGTGGTTGTGAACTCT33340.2138564070327584No Hit
ATTTGCGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG32020.20538938671832405No Hit
ATTTGCGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCT31550.20237461433363907No Hit
ATTTGCGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG31080.19935984194895412No Hit
ATTTGCGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCA30020.1925605680600902No Hit
ATTTGCGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG29120.18678759966388495No Hit
ATTTGCGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCT25860.16587662525096378No Hit
ATTTGCGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA25380.16279770877298763No Hit
ATTTGCGGGGGGGCTGGAGCGATGGCTGAGTGGTTAAAAGCACCGACTGCTC25350.16260527649311413No Hit
ATTTGCGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAAAA24770.15888491908222632No Hit
ATTTGCGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG24440.15676816400361773No Hit
ATTTGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTT24000.15394582389880626No Hit
ATTTGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCT23640.1516366365403242No Hit
ATTTGCGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG23290.14939159327513324No Hit
ATTTGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTC22930.14708240591665117No Hit
ATTTGCGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT22770.14605610042399245No Hit
ATTTGCGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTT21300.13662691871019056No Hit
ATTTGCGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAG21190.1359213336839877No Hit
ATTTGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGC20600.1321368321798087No Hit
ATTTGCGGGGTCGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG20510.1315595353401882No Hit
ATTTGCGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTC20340.13046908575423832No Hit
ATTTGCGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGT19900.12764674564942688No Hit
ATTTGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCTC19830.1271977369963887No Hit
ATTTGCGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACA19740.12662044015676815No Hit
ATTTGCGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC18960.12161720088005697No Hit
ATTTGCGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCAT18580.11917972533499253No Hit
ATTTGCGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC17960.11520279155094004No Hit
ATTTGCGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTC17680.1134067569387873No Hit
ATTTGCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA17680.1134067569387873No Hit
ATTTGCGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT17150.11000711999435532No Hit
ATTTGCGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCTT16970.10885252631511427No Hit
ATTTGCGGGGCTCTCAGCTCCTGAAGGCAAATCCCAATTCCAGAGCAAGGAA16590.10641505077004984No Hit
ATTTGCGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC16570.1062867625834675No Hit
ATTTGCGGGGGGCTGGTGAGATGGCTTAGTGGGTAAGAGCACCCGACTGCTC16260.10429829569144125No Hit
ATTTGCGGGGCTTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAGGA15980.10250226107928852No Hit
ATTTGCGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACTGACTGCTCT15850.10166838786650331No Hit
ATTTGCGGGGGCTCCGCTTCAAGGGAGGCTCTCAGCGATACGTGCCCGGCCC15600.10006478553422407No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAA750.046.00000420
GGTACGA206.312151E-446.010
CGAACGC206.312151E-446.044
TTAATCG206.312151E-446.025
AATCACG253.41772E-546.045
CGACGTT206.312151E-446.045
ACGTTTA206.312151E-446.010
ACCGCAA253.41772E-546.031
CATGACG206.312151E-446.035
TTATGCT206.312151E-446.029
TAGCGGA206.312151E-446.012
GCGTTTA253.41772E-546.021
TAACGGT253.41772E-546.040
ATCACAA1600.046.040
ACTAGAT1300.046.018
CTTATCG206.312151E-446.015
TACGATA206.312151E-446.012
CACTACG206.312151E-446.026
TACGAAC301.8618339E-646.045
GGTATGT253.41772E-546.036
Produced by FastQC (version 0.11.5)