##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1527839_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1558990 Sequences flagged as poor quality 0 Sequence length 52 %GC 57 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.09695315556867 33.0 31.0 34.0 31.0 34.0 2 32.62399438097743 34.0 31.0 34.0 31.0 34.0 3 32.99522832090007 34.0 31.0 34.0 31.0 34.0 4 36.52128301015401 37.0 37.0 37.0 35.0 37.0 5 36.06497026921276 37.0 37.0 37.0 35.0 37.0 6 36.122223362561655 37.0 37.0 37.0 35.0 37.0 7 36.46177012039846 37.0 37.0 37.0 35.0 37.0 8 36.59989993521447 37.0 37.0 37.0 35.0 37.0 9 38.69243035555071 39.0 39.0 39.0 38.0 39.0 10 38.07718458745726 39.0 38.0 39.0 37.0 39.0 11 37.66879518149571 39.0 38.0 39.0 35.0 39.0 12 36.719393325165655 39.0 35.0 39.0 33.0 39.0 13 36.5554775848466 39.0 35.0 39.0 33.0 39.0 14 37.27504474050507 40.0 35.0 40.0 33.0 40.0 15 37.33779883129462 40.0 35.0 40.0 33.0 40.0 16 37.343369745796956 40.0 35.0 40.0 33.0 40.0 17 37.11378135844361 40.0 35.0 40.0 32.0 40.0 18 37.05688747201714 39.0 35.0 40.0 32.0 40.0 19 37.02373010731307 39.0 35.0 40.0 32.0 40.0 20 36.92252548124106 39.0 35.0 40.0 32.0 40.0 21 36.92606495230886 39.0 35.0 40.0 32.0 40.0 22 36.87544564108814 39.0 35.0 40.0 32.0 40.0 23 36.8185132682057 39.0 35.0 40.0 32.0 40.0 24 36.75698689536174 38.0 35.0 40.0 32.0 40.0 25 36.68677990237269 38.0 35.0 40.0 32.0 40.0 26 36.481202573461026 38.0 35.0 40.0 32.0 40.0 27 36.301633750056126 38.0 35.0 40.0 31.0 40.0 28 36.05981180123028 37.0 35.0 40.0 31.0 40.0 29 35.87624423504962 37.0 35.0 40.0 31.0 40.0 30 35.67513261791288 37.0 34.0 40.0 30.0 40.0 31 35.43587322561402 36.0 34.0 40.0 30.0 40.0 32 35.30889806862135 36.0 34.0 40.0 30.0 40.0 33 35.06604339989352 35.0 34.0 39.0 30.0 40.0 34 34.56059115196377 35.0 33.0 39.0 27.0 40.0 35 34.38998133406885 35.0 33.0 39.0 27.0 40.0 36 34.481826054047815 35.0 33.0 39.0 28.0 40.0 37 34.421519060417324 35.0 33.0 38.0 29.0 40.0 38 34.35048140142015 35.0 33.0 38.0 29.0 40.0 39 34.21852866278809 35.0 33.0 38.0 29.0 40.0 40 33.93790082040295 35.0 33.0 37.0 27.0 40.0 41 33.737866182592576 35.0 33.0 37.0 27.0 40.0 42 33.6720088005696 35.0 33.0 37.0 27.0 40.0 43 33.53321894303363 35.0 33.0 37.0 27.0 40.0 44 33.45507475994073 35.0 33.0 36.0 27.0 40.0 45 33.32512139269655 35.0 33.0 36.0 26.0 39.0 46 33.13529272156973 35.0 33.0 36.0 25.0 39.0 47 32.938287609285496 35.0 32.0 35.0 25.0 39.0 48 32.75478547007999 35.0 32.0 35.0 24.0 39.0 49 33.119807696008316 35.0 33.0 35.0 26.0 39.0 50 33.34889640087493 35.0 33.0 35.0 27.0 39.0 51 33.381104432998285 35.0 33.0 35.0 27.0 39.0 52 32.981169218532514 35.0 33.0 35.0 26.0 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 1.0 12 0.0 13 0.0 14 8.0 15 23.0 16 55.0 17 139.0 18 364.0 19 699.0 20 1331.0 21 2128.0 22 3546.0 23 5220.0 24 7207.0 25 9841.0 26 12885.0 27 16933.0 28 22187.0 29 28223.0 30 36232.0 31 46781.0 32 62569.0 33 92668.0 34 200333.0 35 220385.0 36 272732.0 37 244688.0 38 219176.0 39 52636.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 100.0 0.0 0.0 2 0.0 0.0 100.0 0.0 3 0.0 0.0 100.0 0.0 4 0.0 0.0 100.0 0.0 5 100.0 0.0 0.0 0.0 6 0.0 0.0 0.0 100.0 7 99.18402298924303 0.7449053553903489 0.02232214446532691 0.04874951090128866 8 99.63418623596047 0.26664699581139073 0.03944861737406911 0.0597181508540786 9 98.86067261496225 0.6862776541222202 0.22136126594782518 0.23168846496770343 10 70.25144484570139 21.345294068595695 3.138249764270457 5.265011321432466 11 44.318052072174936 23.518111084740763 14.046016972527084 18.11781987055722 12 37.50954143387706 13.697650401862743 23.968274331458186 24.824533832802008 13 27.066947190167994 14.041142021436956 27.605693429720525 31.286217358674527 14 25.81350746316525 14.643455057441034 31.38724430560812 28.155793173785593 15 29.629375428963623 15.694391881923552 28.528855220367035 26.14737746874579 16 36.85514339412055 15.818061693788927 23.518560093393802 23.808234818696718 17 34.44063143445436 18.197615122611435 23.44896375217288 23.912789690761326 18 34.93838959839383 18.270354524403622 22.24529984156409 24.545956035638458 19 32.58968947844438 21.80424505609401 20.83881230796862 24.76725315749299 20 33.03273273080649 21.999243099699164 21.341573711184804 23.62645045830955 21 35.38784726008505 21.961911237403704 22.03792198795374 20.6123195145575 22 31.29057915701833 19.35156736091957 25.508887164125493 23.84896631793661 23 34.62741903411825 17.231156068993386 24.439348552588534 23.702076344299837 24 37.565090218667216 15.300611293209066 21.938306211072554 25.195992277051165 25 31.325730120141888 16.207352195973034 21.527719869915778 30.9391978139693 26 27.02602325864823 17.116594718375357 24.611062290328995 31.24631973264742 27 27.291579804873667 16.438784084567573 23.141777689401472 33.127858421157285 28 28.079205126395934 21.657739947016978 20.172098602300206 30.09095632428688 29 32.78507238660928 21.118095690158373 19.87498316217551 26.221848761056837 30 33.00380374473217 17.367462267237123 23.419008460605905 26.20972552742481 31 36.13057171630351 17.544628252907334 20.976786252637925 25.34801377815124 32 40.27594788933861 16.39157403190527 20.499490054458334 22.83298802429778 33 38.45156158795117 16.73994060256961 22.45857895175723 22.34991885772199 34 30.94817798703006 20.087492543249155 28.483441202316882 20.480888267403895 35 28.883828632640363 26.19894931975189 25.539163176158926 19.37805887144882 36 29.52687316788434 29.238930333100278 19.727515891699113 21.506680607316277 37 32.88917825002085 27.28182990269341 18.159256954823316 21.66973489246243 38 33.1168897812045 27.351041379354584 17.618393960192176 21.913674879248745 39 34.90548367853546 23.733763526385673 17.69870236499272 23.662050430086147 40 31.19263112656271 20.72014573537996 18.09190565686759 29.995317481189744 41 25.35891827401074 23.696624096370087 20.26837888633025 30.676078743288926 42 25.728067530901416 20.81418097614481 19.701152669356443 33.75659882359733 43 24.581876727881514 17.415826913578663 24.86366172970962 33.138634628830204 44 26.77002418232317 17.405499714558783 22.642223490849844 33.1822526122682 45 29.946054817542127 19.175555968928602 22.4108557463486 28.467533467180672 46 28.282157037569196 22.464865072899762 20.86831859088256 28.384659298648486 47 25.9933033566604 20.553627669196082 23.888543223497262 29.564525750646254 48 28.91442536514025 17.395172515538906 27.498829370297432 26.191572749023408 49 30.306480477745207 17.389078826676247 25.031206101386154 27.27323459419239 50 26.899146242118295 17.83860063246076 25.25154106184132 30.010712063579625 51 24.856926599914047 18.32083592582377 26.928331804565776 29.89390566969641 52 25.084189122444663 19.249834828959774 26.181117261816944 29.484858786778616 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 4.5 4 9.0 5 4.5 6 0.0 7 2.0 8 4.0 9 51.0 10 98.0 11 461.5 12 825.0 13 1763.5 14 1885.0 15 1068.0 16 1105.0 17 1142.0 18 1230.0 19 1318.0 20 1625.0 21 1932.0 22 2114.5 23 2297.0 24 2699.0 25 3101.0 26 3321.0 27 3541.0 28 4811.5 29 6082.0 30 5983.0 31 5884.0 32 6935.0 33 7986.0 34 9418.0 35 10850.0 36 11180.5 37 11511.0 38 13610.0 39 17079.5 40 18450.0 41 19245.0 42 20040.0 43 23831.0 44 27622.0 45 29210.0 46 30798.0 47 37395.5 48 43993.0 49 47831.5 50 51670.0 51 61227.0 52 70784.0 53 80917.0 54 91050.0 55 111588.5 56 132127.0 57 160684.0 58 189241.0 59 190060.5 60 190880.0 61 196439.5 62 201999.0 63 161826.5 64 108840.0 65 96026.0 66 79612.0 67 63198.0 68 54382.5 69 45567.0 70 38079.5 71 30592.0 72 31483.0 73 32374.0 74 24344.0 75 16314.0 76 10793.0 77 5272.0 78 3676.5 79 2081.0 80 1513.0 81 945.0 82 550.0 83 155.0 84 110.0 85 65.0 86 41.0 87 17.0 88 14.5 89 8.5 90 5.0 91 2.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 52 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 52 1558990.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 6.868568726909132 #Duplication Level Percentage of deduplicated Percentage of total 1 66.88968488854744 4.594363977782832 2 10.239791291418081 1.4066542046862138 3 4.748285317283649 0.9784177211020882 4 2.3824727472832645 0.6545671121881245 5 1.3565002744132026 0.465860768143909 6 0.8426679325876615 0.34727535653644703 7 0.567072122155929 0.2726481690899546 8 0.42039940126757747 0.23100337442862443 9 0.2980164623732534 0.18422518982049177 >10 4.80282956714708 9.305136688079797 >50 4.135795855607334 20.708554010971813 >100 3.0707840472964323 37.78490082515422 >500 0.1597517712466352 7.263901052750728 >1k 0.07847455429659274 9.853898033300343 >5k 0.005605325306899481 2.752982693469766 >10k+ 0.001868441768966494 3.195610822494647 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ATTTGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC 30478 1.9549836753282572 No Hit ATTTGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT 19323 1.239456314665264 No Hit ATTTGCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG 9951 0.6382978723404255 No Hit ATTTGCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT 8094 0.5191822910987242 No Hit ATTTGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC 6558 0.4206569638034881 No Hit ATTTGCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT 6341 0.4067376955593044 No Hit ATTTGCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG 6287 0.4032739145215813 No Hit ATTTGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC 5672 0.36382529714751216 No Hit ATTTGCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC 4854 0.3113554288353357 No Hit ATTTGCGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC 4562 0.29262535359431424 No Hit ATTTGCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC 4080 0.26170790062797067 No Hit ATTTGCGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA 3877 0.24868664968986331 No Hit ATTTGCGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT 3853 0.24714719145087524 No Hit ATTTGCGGGGGAGTGGCCTTGTTCTCGATACTTCGTTGTGGTTGTGAACTCT 3334 0.2138564070327584 No Hit ATTTGCGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG 3202 0.20538938671832405 No Hit ATTTGCGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCT 3155 0.20237461433363907 No Hit ATTTGCGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG 3108 0.19935984194895412 No Hit ATTTGCGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCA 3002 0.1925605680600902 No Hit ATTTGCGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG 2912 0.18678759966388495 No Hit ATTTGCGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCT 2586 0.16587662525096378 No Hit ATTTGCGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA 2538 0.16279770877298763 No Hit ATTTGCGGGGGGGCTGGAGCGATGGCTGAGTGGTTAAAAGCACCGACTGCTC 2535 0.16260527649311413 No Hit ATTTGCGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAAAA 2477 0.15888491908222632 No Hit ATTTGCGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG 2444 0.15676816400361773 No Hit ATTTGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTT 2400 0.15394582389880626 No Hit ATTTGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCT 2364 0.1516366365403242 No Hit ATTTGCGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG 2329 0.14939159327513324 No Hit ATTTGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTC 2293 0.14708240591665117 No Hit ATTTGCGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT 2277 0.14605610042399245 No Hit ATTTGCGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTT 2130 0.13662691871019056 No Hit ATTTGCGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAG 2119 0.1359213336839877 No Hit ATTTGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGC 2060 0.1321368321798087 No Hit ATTTGCGGGGTCGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG 2051 0.1315595353401882 No Hit ATTTGCGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTC 2034 0.13046908575423832 No Hit ATTTGCGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGT 1990 0.12764674564942688 No Hit ATTTGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCTC 1983 0.1271977369963887 No Hit ATTTGCGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACA 1974 0.12662044015676815 No Hit ATTTGCGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC 1896 0.12161720088005697 No Hit ATTTGCGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCAT 1858 0.11917972533499253 No Hit ATTTGCGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC 1796 0.11520279155094004 No Hit ATTTGCGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTC 1768 0.1134067569387873 No Hit ATTTGCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA 1768 0.1134067569387873 No Hit ATTTGCGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT 1715 0.11000711999435532 No Hit ATTTGCGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCTT 1697 0.10885252631511427 No Hit ATTTGCGGGGCTCTCAGCTCCTGAAGGCAAATCCCAATTCCAGAGCAAGGAA 1659 0.10641505077004984 No Hit ATTTGCGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC 1657 0.1062867625834675 No Hit ATTTGCGGGGGGCTGGTGAGATGGCTTAGTGGGTAAGAGCACCCGACTGCTC 1626 0.10429829569144125 No Hit ATTTGCGGGGCTTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAGGA 1598 0.10250226107928852 No Hit ATTTGCGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACTGACTGCTCT 1585 0.10166838786650331 No Hit ATTTGCGGGGGCTCCGCTTCAAGGGAGGCTCTCAGCGATACGTGCCCGGCCC 1560 0.10006478553422407 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 5.772968396205235E-4 0.0 0.0 0.0 0.0 8 0.002950628291393787 0.0 0.0 0.0 0.0 9 0.004618374716964188 0.0 0.0 0.0 0.0 10 0.009621613993675393 0.0 0.0 0.0 0.0 11 0.017318905188615705 0.0 0.0 0.0 0.0 12 0.021167550786085863 0.0 0.0 0.0 0.0 13 0.02687637508899993 0.0 0.0 0.0 0.0 14 0.03207204664558464 0.0 0.0 0.0 0.0 15 0.03867888825457508 0.0 0.0 0.0 0.0 16 0.04714590856900942 0.0 0.0 0.0 0.0 17 0.05516392023040558 0.0 0.0 0.0 0.0 18 0.06273292323876356 0.0 0.0 0.0 0.0 19 0.07190552857940076 0.0 0.0 0.0 0.0 20 0.07947453158775875 0.0 0.0 0.0 0.0 21 0.08685110231624321 0.0 0.0 0.0 0.0 22 0.09435596123131001 0.0 0.0 0.0 0.0 23 0.10205325242625032 0.0 0.0 0.0 0.0 24 0.10910910268827895 0.0 0.0 0.0 0.0 25 0.11770441118929563 0.0 0.0 0.0 0.0 26 0.1250809819177801 0.0 0.0 0.0 0.0 27 0.13207268808651756 0.0 0.0 0.0 0.0 28 0.14066799658753423 0.0 0.0 0.0 0.0 29 0.147403126383107 0.0 0.0 0.0 0.0 30 0.15317609477931224 0.0 0.0 0.0 0.0 31 0.16234870011994945 0.0 0.0 0.0 0.0 32 0.171649593647169 0.0 0.0 0.0 0.0 33 0.1804373344280592 0.0 0.0 0.0 0.0 34 0.1898023720485699 0.0 0.0 0.0 0.0 35 0.20186146158730972 0.0 0.0 0.0 0.0 36 0.2159090180180758 0.0 0.0 0.0 0.0 37 0.22790396346352446 0.0 0.0 0.0 0.0 38 0.23919332388277026 0.0 0.0 0.0 0.0 39 0.24862250559657215 0.0 0.0 0.0 0.0 40 0.2589497046164504 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTATAA 75 0.0 46.000004 20 GGTACGA 20 6.312151E-4 46.0 10 CGAACGC 20 6.312151E-4 46.0 44 TTAATCG 20 6.312151E-4 46.0 25 AATCACG 25 3.41772E-5 46.0 45 CGACGTT 20 6.312151E-4 46.0 45 ACGTTTA 20 6.312151E-4 46.0 10 ACCGCAA 25 3.41772E-5 46.0 31 CATGACG 20 6.312151E-4 46.0 35 TTATGCT 20 6.312151E-4 46.0 29 TAGCGGA 20 6.312151E-4 46.0 12 GCGTTTA 25 3.41772E-5 46.0 21 TAACGGT 25 3.41772E-5 46.0 40 ATCACAA 160 0.0 46.0 40 ACTAGAT 130 0.0 46.0 18 CTTATCG 20 6.312151E-4 46.0 15 TACGATA 20 6.312151E-4 46.0 12 CACTACG 20 6.312151E-4 46.0 26 TACGAAC 30 1.8618339E-6 46.0 45 GGTATGT 25 3.41772E-5 46.0 36 >>END_MODULE