FastQCFastQC Report
Fri 17 Jun 2016
SRR1527838_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527838_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1568399
Sequences flagged as poor quality0
Sequence length52
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC291751.8601771615513654No Hit
ATTTGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT188151.1996309612541196No Hit
ATTTGCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG96040.6123441802755549No Hit
ATTTGCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT79880.5093091745149034No Hit
ATTTGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC62060.39569012732091774No Hit
ATTTGCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT60370.3849148080303545No Hit
ATTTGCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG58750.3745858037399922No Hit
ATTTGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC54490.3474243480134838No Hit
ATTTGCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC45390.2894033979873744No Hit
ATTTGCGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC44330.28264491369861877No Hit
ATTTGCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC38600.24611084296789273No Hit
ATTTGCGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA38330.24438934225283235No Hit
ATTTGCGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT36120.23029854010363435No Hit
ATTTGCGGGGGAGTGGCCTTGTTCTCGATACTTCGTTGTGGTTGTGAACTCT32320.20607001152130291No Hit
ATTTGCGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG30660.1954859700879687No Hit
ATTTGCGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCT30030.19146913508616112No Hit
ATTTGCGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCA29580.18859996722772715No Hit
ATTTGCGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG29530.18828117079901224No Hit
ATTTGCGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG27770.17705953650824824No Hit
ATTTGCGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCT25450.16226738221587747No Hit
ATTTGCGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA23830.15193837792551512No Hit
ATTTGCGGGGGGGCTGGAGCGATGGCTGAGTGGTTAAAAGCACCGACTGCTC23540.15008935863896877No Hit
ATTTGCGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG23070.14709267220904884No Hit
ATTTGCGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG22960.1463913200658761No Hit
ATTTGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCT22780.1452436529225025No Hit
ATTTGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTT22710.14479733792230168No Hit
ATTTGCGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAAAA22520.1435859114931851No Hit
ATTTGCGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT22240.14180065149238172No Hit
ATTTGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTC22120.141035540063466No Hit
ATTTGCGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAG21410.1365086307757146No Hit
ATTTGCGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTT20640.13159916577350533No Hit
ATTTGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGC19980.12739105291446884No Hit
ATTTGCGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGT19970.12732729362872586No Hit
ATTTGCGGGGTCGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG19870.12668970077129607No Hit
ATTTGCGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTC19450.12401181077009103No Hit
ATTTGCGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC18800.1198674571967975No Hit
ATTTGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCTC18460.11769964148153626No Hit
ATTTGCGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC18140.11565934433776098No Hit
ATTTGCGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACA17790.11342776933675677No Hit
ATTTGCGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCAT17270.11011228647812196No Hit
ATTTGCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA17230.10985724933515004No Hit
ATTTGCGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCTT16630.1060316921905714No Hit
ATTTGCGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC16230.10348132076085231No Hit
ATTTGCGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTC16220.10341756147510933No Hit
ATTTGCGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTT15890.10131350504559108No Hit
ATTTGCGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGA15790.1006759121881613No Hit
ATTTGCGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT15780.10061215290241832No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG253.4177305E-546.019
CGAACAC301.8618412E-646.044
CTCGTTA206.3121616E-446.044
ACGTTTA206.3121616E-446.010
CAACGAA253.4177305E-546.022
GCGAAAT501.6370905E-1146.039
ACCGCAA253.4177305E-546.031
TAGCGTC301.8618412E-646.014
ACCGATA206.3121616E-446.022
CACGATA301.8618412E-646.029
TACGGTA206.3121616E-446.011
CGACATA301.8618412E-646.039
TCGATAT253.4177305E-546.029
ACGGGTA206.3121616E-446.030
CGTTATA206.3121616E-446.046
ATACTAG206.3121616E-446.010
ACTAGGC206.3121616E-446.011
ATATCCC206.3121616E-446.014
CTTATAA501.6370905E-1146.020
ACGGATA301.8618412E-646.043
Produced by FastQC (version 0.11.5)