##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527838_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1568399
Sequences flagged as poor quality	0
Sequence length	52
%GC	57
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.06094048772028	31.0	31.0	34.0	31.0	34.0
2	32.60518911322948	34.0	31.0	34.0	31.0	34.0
3	32.98069241309131	34.0	31.0	34.0	31.0	34.0
4	36.51416826968138	37.0	37.0	37.0	35.0	37.0
5	36.07046548741742	37.0	37.0	37.0	35.0	37.0
6	36.06469590965054	37.0	37.0	37.0	35.0	37.0
7	36.411527296306616	37.0	37.0	37.0	35.0	37.0
8	36.562865061760434	37.0	37.0	37.0	35.0	37.0
9	38.639675873295	39.0	39.0	39.0	38.0	39.0
10	37.971043720379825	39.0	38.0	39.0	37.0	39.0
11	37.62492835050265	39.0	38.0	39.0	35.0	39.0
12	36.65433540827302	39.0	35.0	39.0	33.0	39.0
13	36.33945507488847	39.0	35.0	39.0	32.0	39.0
14	37.18944796572811	40.0	35.0	40.0	33.0	40.0
15	37.27621606491716	40.0	35.0	40.0	33.0	40.0
16	37.312069824069006	40.0	35.0	40.0	33.0	40.0
17	37.11264289252926	40.0	35.0	40.0	32.0	40.0
18	37.077426088641985	39.0	35.0	40.0	32.0	40.0
19	36.98810379246608	39.0	35.0	40.0	32.0	40.0
20	36.90181898866296	39.0	35.0	40.0	32.0	40.0
21	36.96974303095067	39.0	35.0	40.0	32.0	40.0
22	36.8138394630448	39.0	35.0	40.0	32.0	40.0
23	36.771258461654206	39.0	35.0	40.0	32.0	40.0
24	36.754292115718	38.0	35.0	40.0	32.0	40.0
25	36.653672949294155	38.0	35.0	40.0	32.0	40.0
26	36.40727200157613	38.0	35.0	40.0	32.0	40.0
27	36.22223235286429	38.0	35.0	40.0	31.0	40.0
28	35.9946231794333	37.0	35.0	40.0	31.0	40.0
29	35.80548317105532	37.0	35.0	40.0	31.0	40.0
30	35.59928181540539	37.0	34.0	40.0	30.0	40.0
31	35.37951758449221	36.0	34.0	40.0	30.0	40.0
32	35.2520283422777	36.0	34.0	39.0	30.0	40.0
33	34.774916331877286	35.0	34.0	39.0	29.0	40.0
34	34.41689455298046	35.0	33.0	39.0	26.0	40.0
35	34.33171342241356	35.0	33.0	39.0	27.0	40.0
36	34.296341683461925	35.0	33.0	39.0	28.0	40.0
37	34.306765689088046	35.0	33.0	38.0	28.0	40.0
38	33.9817769585418	35.0	33.0	38.0	27.0	40.0
39	34.006718315938734	35.0	33.0	38.0	28.0	40.0
40	33.796091428265385	35.0	33.0	37.0	27.0	40.0
41	33.41230005884982	35.0	33.0	37.0	26.0	40.0
42	33.479165696994194	35.0	33.0	37.0	26.0	40.0
43	33.4587933300136	35.0	33.0	36.0	26.0	40.0
44	33.379590270077955	35.0	33.0	36.0	26.0	40.0
45	33.24792224427585	35.0	33.0	36.0	26.0	39.0
46	32.88854813092842	35.0	32.0	35.0	24.0	39.0
47	32.618123959528155	35.0	32.0	35.0	23.0	39.0
48	32.59164727853053	35.0	32.0	35.0	23.0	39.0
49	32.49597455749462	34.0	31.0	35.0	24.0	39.0
50	32.48387495783917	34.0	32.0	35.0	24.0	38.0
51	32.89941717636902	35.0	33.0	35.0	26.0	39.0
52	32.75038940983767	35.0	32.0	35.0	25.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	1.0
14	12.0
15	28.0
16	68.0
17	168.0
18	373.0
19	763.0
20	1358.0
21	2456.0
22	3648.0
23	5623.0
24	7702.0
25	10347.0
26	13937.0
27	18177.0
28	23292.0
29	29871.0
30	38740.0
31	49391.0
32	66949.0
33	101770.0
34	209236.0
35	222101.0
36	271765.0
37	238849.0
38	207362.0
39	44411.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	0.0	0.0	100.0	0.0
3	0.0	0.0	100.0	0.0
4	0.0	0.0	100.0	0.0
5	100.0	0.0	0.0	0.0
6	0.0	0.0	0.0	100.0
7	99.17686762105816	0.7481514589080968	0.02779904858393814	0.04718187144980327
8	99.62885719769012	0.27225215012251347	0.04048714644679065	0.05840350574056729
9	98.83849709162018	0.6923620838829916	0.2389060436789363	0.23023478081789137
10	70.23844060089301	21.271564187429345	3.1779540792872223	5.312041132390418
11	44.362180797105836	23.499313631288977	14.017670248450809	18.120835323154374
12	37.62626729550325	13.649332854713627	23.969028289357492	24.755371560425633
13	27.306508101573645	14.076966384191778	27.517615096668642	31.09891041756594
14	25.75460708658957	14.56421484583961	31.393350799127006	28.28782726844381
15	29.545479179724037	15.684784292772438	28.532790444268326	26.236946083235196
16	36.89858256731865	15.813705568544739	23.423758877683547	23.863952986453064
17	34.49715282909514	18.291518931088326	23.3828891755223	23.828439064294226
18	34.955199537872694	18.225783107487317	22.281830070026825	24.537187284613164
19	32.60388459824318	21.847565574831403	20.810074477221676	24.73847534970374
20	33.06754212416611	21.99586967346957	21.36886085747313	23.567727344891193
21	35.36982617305927	21.97323512703081	21.964882660598484	20.692056039311424
22	31.378686163406122	19.444605613750074	25.393219454998377	23.783488767845427
23	34.690088427753395	17.16820783486855	24.405779396696886	23.735924340681166
24	37.63423720622112	15.318168399750318	21.85878720912217	25.18880718490639
25	31.41585782699428	16.183254388711035	21.5044130989627	30.896474685331988
26	26.95289910284309	17.20824866631514	24.626960358939275	31.21189187190249
27	27.23516146082725	16.493762110279338	23.156862507563446	33.11421392132996
28	28.127090109085763	21.61191125472536	20.171971545505958	30.08902709068292
29	32.88302275122593	21.18102600167432	19.791137331763153	26.144813915336595
30	33.12798592705045	17.29323979421053	23.39417456909881	26.18459970964021
31	36.11192049982179	17.547766862896495	20.9896206258739	25.350692011407812
32	40.331956345292234	16.34029350949599	20.45187480991763	22.875875335294143
33	38.353569467973394	16.810645760421934	22.445500156529047	22.39028461507563
34	31.010858843954885	20.103366554046513	28.395771739206673	20.490002862791933
35	28.941296188023586	26.253651016099855	25.42643804287047	19.37861475300609
36	29.70506867193871	29.2380319038714	19.611017349539246	21.445882074650648
37	32.83214284120304	27.334562187300556	18.115096987437507	21.718197984058904
38	33.17191607492736	27.340746838017623	17.50766227216416	21.979674814890853
39	34.87836959855241	23.769844280696432	17.649717960799517	23.702068159951644
40	31.175230282600282	20.74414737576344	18.068425190273647	30.012197151362628
41	25.615866880812852	23.71137701567012	20.247079984111185	30.425676119405843
42	25.733311485151418	20.787312412211435	19.765633617465962	33.71374248517118
43	24.58079863606136	17.342398203518364	24.83118135117403	33.245621809246245
44	26.75205735275271	17.406986359976003	22.690399573067825	33.15055671420347
45	29.968394522057206	19.165786257196032	22.358978805775827	28.506840414970934
46	28.5103471756868	22.384036205072817	20.846225992237944	28.25939062700244
47	26.20493892179222	20.673884642874675	23.77794170998579	29.343234725347312
48	28.962783067318966	17.359039377097282	27.47649035736442	26.201687198219332
49	30.35139655151527	17.29878685207017	25.02749619197666	27.3223204044379
50	27.02207792787422	17.785780276575032	25.15189055846121	30.040251237089542
51	24.87358127619311	18.26078695536021	26.94212378355253	29.923507984894147
52	25.165280008467235	19.11484258788739	26.239815251093628	29.480062152551746
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	3.0
4	6.0
5	3.5
6	1.0
7	5.0
8	9.0
9	58.5
10	108.0
11	440.0
12	772.0
13	1703.0
14	1918.5
15	1203.0
16	1215.5
17	1228.0
18	1238.5
19	1249.0
20	1568.5
21	1888.0
22	2076.5
23	2265.0
24	2644.5
25	3024.0
26	3279.0
27	3534.0
28	4731.5
29	5929.0
30	5937.0
31	5945.0
32	6888.5
33	7832.0
34	9539.5
35	11247.0
36	11371.0
37	11495.0
38	13600.0
39	17191.0
40	18677.0
41	19398.5
42	20120.0
43	23764.0
44	27408.0
45	29252.5
46	31097.0
47	37392.0
48	43687.0
49	48011.0
50	52335.0
51	61536.0
52	70737.0
53	81244.0
54	91751.0
55	112247.5
56	132744.0
57	161123.0
58	189502.0
59	191116.5
60	192731.0
61	197213.0
62	201695.0
63	162537.0
64	110086.5
65	96794.0
66	80884.0
67	64974.0
68	55233.0
69	45492.0
70	38292.5
71	31093.0
72	31914.0
73	32735.0
74	24658.5
75	16582.0
76	11037.0
77	5492.0
78	3817.5
79	2143.0
80	1534.0
81	925.0
82	534.0
83	143.0
84	102.5
85	62.0
86	37.5
87	13.0
88	10.5
89	6.0
90	4.0
91	3.0
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1568399.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	9.64930330191027
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.31092517665586	6.977500490712919
2	10.104509330485257	1.9500295049366907
3	3.89233789773347	1.1267504678625018
4	2.038340941466809	0.7867427990765831
5	1.0761534941762778	0.5192065732358716
6	0.6622135096254409	0.3833939402999013
7	0.45066888521848486	0.3044048533544863
8	0.3274553132121679	0.25277725080049873
9	0.24761089100127365	0.2150345329314781
>10	3.7104692193544784	9.942625511540266
>50	2.9592265360836683	20.60543539963569
>100	2.0602210061341997	35.49513508672945
>500	0.10167324445857087	6.494852033315398
>1k	0.05284333100149408	9.192036426694683
>5k	0.004013417544417272	2.6565859205297686
>10k+	0.0013378058481390907	3.0974892083438275
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
ATTTGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	29175	1.8601771615513654	No Hit
ATTTGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	18815	1.1996309612541196	No Hit
ATTTGCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	9604	0.6123441802755549	No Hit
ATTTGCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	7988	0.5093091745149034	No Hit
ATTTGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC	6206	0.39569012732091774	No Hit
ATTTGCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT	6037	0.3849148080303545	No Hit
ATTTGCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	5875	0.3745858037399922	No Hit
ATTTGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	5449	0.3474243480134838	No Hit
ATTTGCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	4539	0.2894033979873744	No Hit
ATTTGCGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	4433	0.28264491369861877	No Hit
ATTTGCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	3860	0.24611084296789273	No Hit
ATTTGCGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA	3833	0.24438934225283235	No Hit
ATTTGCGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	3612	0.23029854010363435	No Hit
ATTTGCGGGGGAGTGGCCTTGTTCTCGATACTTCGTTGTGGTTGTGAACTCT	3232	0.20607001152130291	No Hit
ATTTGCGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG	3066	0.1954859700879687	No Hit
ATTTGCGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCT	3003	0.19146913508616112	No Hit
ATTTGCGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCA	2958	0.18859996722772715	No Hit
ATTTGCGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG	2953	0.18828117079901224	No Hit
ATTTGCGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG	2777	0.17705953650824824	No Hit
ATTTGCGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCT	2545	0.16226738221587747	No Hit
ATTTGCGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA	2383	0.15193837792551512	No Hit
ATTTGCGGGGGGGCTGGAGCGATGGCTGAGTGGTTAAAAGCACCGACTGCTC	2354	0.15008935863896877	No Hit
ATTTGCGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG	2307	0.14709267220904884	No Hit
ATTTGCGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG	2296	0.1463913200658761	No Hit
ATTTGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCT	2278	0.1452436529225025	No Hit
ATTTGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTT	2271	0.14479733792230168	No Hit
ATTTGCGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAAAA	2252	0.1435859114931851	No Hit
ATTTGCGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT	2224	0.14180065149238172	No Hit
ATTTGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTC	2212	0.141035540063466	No Hit
ATTTGCGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAG	2141	0.1365086307757146	No Hit
ATTTGCGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTT	2064	0.13159916577350533	No Hit
ATTTGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGC	1998	0.12739105291446884	No Hit
ATTTGCGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGT	1997	0.12732729362872586	No Hit
ATTTGCGGGGTCGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG	1987	0.12668970077129607	No Hit
ATTTGCGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTC	1945	0.12401181077009103	No Hit
ATTTGCGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	1880	0.1198674571967975	No Hit
ATTTGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCTC	1846	0.11769964148153626	No Hit
ATTTGCGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC	1814	0.11565934433776098	No Hit
ATTTGCGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACA	1779	0.11342776933675677	No Hit
ATTTGCGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCAT	1727	0.11011228647812196	No Hit
ATTTGCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	1723	0.10985724933515004	No Hit
ATTTGCGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCTT	1663	0.1060316921905714	No Hit
ATTTGCGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC	1623	0.10348132076085231	No Hit
ATTTGCGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTC	1622	0.10341756147510933	No Hit
ATTTGCGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTT	1589	0.10131350504559108	No Hit
ATTTGCGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGA	1579	0.1006759121881613	No Hit
ATTTGCGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT	1578	0.10061215290241832	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0013389450006025252	0.0	0.0	0.0	0.0
8	0.003443001430120779	0.0	0.0	0.0	0.0
9	0.005419539288153079	0.0	0.0	0.0	0.0
10	0.011157875005021044	0.0	0.0	0.0	0.0
11	0.01734252572208985	0.0	0.0	0.0	0.0
12	0.02033921215200979	0.0	0.0	0.0	0.0
13	0.02741649286948028	0.0	0.0	0.0	0.0
14	0.03207092072871763	0.0	0.0	0.0	0.0
15	0.038383090017272394	0.0	0.0	0.0	0.0
16	0.0459704450206867	0.0	0.0	0.0	0.0
17	0.052920207166671236	0.0	0.0	0.0	0.0
18	0.059232376455226	0.0	0.0	0.0	0.0
19	0.06981641788856024	0.0	0.0	0.0	0.0
20	0.07976286646446472	0.0	0.0	0.0	0.0
21	0.087413980753622	0.0	0.0	0.0	0.0
22	0.09519261361426525	0.0	0.0	0.0	0.0
23	0.10284372790342254	0.0	0.0	0.0	0.0
24	0.11125995362149554	0.0	0.0	0.0	0.0
25	0.1178909193387652	0.0	0.0	0.0	0.0
26	0.12515947791346463	0.0	0.0	0.0	0.0
27	0.13210924005944916	0.0	0.0	0.0	0.0
28	0.1393777986341486	0.0	0.0	0.0	0.0
29	0.14594500506567526	0.0	0.0	0.0	0.0
30	0.15359611935483253	0.0	0.0	0.0	0.0
31	0.16009956650061624	0.0	0.0	0.0	0.0
32	0.1700460150765207	0.0	0.0	0.0	0.0
33	0.1762306657935895	0.0	0.0	0.0	0.0
34	0.1863683922267229	0.0	0.0	0.0	0.0
35	0.19701619294580014	0.0	0.0	0.0	0.0
36	0.21193586580965684	0.0	0.0	0.0	0.0
37	0.2230299815289349	0.0	0.0	0.0	0.0
38	0.23348650439078322	0.0	0.0	0.0	0.0
39	0.24324167510945877	0.0	0.0	0.0	0.0
40	0.25076527082713007	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATCACG	25	3.4177305E-5	46.0	19
CGAACAC	30	1.8618412E-6	46.0	44
CTCGTTA	20	6.3121616E-4	46.0	44
ACGTTTA	20	6.3121616E-4	46.0	10
CAACGAA	25	3.4177305E-5	46.0	22
GCGAAAT	50	1.6370905E-11	46.0	39
ACCGCAA	25	3.4177305E-5	46.0	31
TAGCGTC	30	1.8618412E-6	46.0	14
ACCGATA	20	6.3121616E-4	46.0	22
CACGATA	30	1.8618412E-6	46.0	29
TACGGTA	20	6.3121616E-4	46.0	11
CGACATA	30	1.8618412E-6	46.0	39
TCGATAT	25	3.4177305E-5	46.0	29
ACGGGTA	20	6.3121616E-4	46.0	30
CGTTATA	20	6.3121616E-4	46.0	46
ATACTAG	20	6.3121616E-4	46.0	10
ACTAGGC	20	6.3121616E-4	46.0	11
ATATCCC	20	6.3121616E-4	46.0	14
CTTATAA	50	1.6370905E-11	46.0	20
ACGGATA	30	1.8618412E-6	46.0	43
>>END_MODULE