FastQCFastQC Report
Fri 17 Jun 2016
SRR1527837_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527837_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2054751
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACATGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGGACATGGGA232071.1294312546873075No Hit
GGACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA155390.7562473506522202No Hit
GGACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA108780.5294072128447681No Hit
GGACATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA80190.39026626583951046No Hit
GGACATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC69520.33833783266196243No Hit
GGACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC47500.23117156288036847No Hit
GGACATGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC41720.20304163375513626No Hit
GGACATGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAA41640.20265229217554828Illumina Single End Adapter 2 (100% over 34bp)
GGACATGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT39070.19014469393128414No Hit
GGACATGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA38530.1875166382690652No Hit
GGACATGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC34660.16868223935649623No Hit
GGACATGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC33960.16527550053510134No Hit
GGACATGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA27270.13271681094205576Illumina Single End Adapter 2 (97% over 34bp)
GGACATGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA27030.1315487862032918Illumina Single End Adapter 2 (97% over 34bp)
GGACATGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTT23020.11203303952644382No Hit
GGACATGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22060.10736094057138797No Hit
GGACATGGGAAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCG21700.10560890346324203Illumina Single End Adapter 2 (100% over 23bp)
GGACATGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA21110.10273750931378059Illumina Single End Adapter 2 (97% over 34bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTAG351.4468242E-744.014
TATATCG207.8579324E-444.019
TACGGTT207.8579324E-444.042
TAACGCG302.5286936E-644.037
CGTTCGA408.3182385E-944.021
ATGTACG254.4440676E-544.032
CCGTATA207.8579324E-444.012
TTACGGT207.8579324E-444.041
CGTCATA254.4440676E-544.026
CGATATT254.4440676E-544.010
ATGGGAT156750.041.4456145
ATGGGGT185150.041.0294385
ATGGGAC88200.040.83225
ATGGGAG185350.040.5459945
ATGGGGC183400.040.4852755
CATGGGG1037500.040.2043384
GGACATG2247500.039.9377061
ATGGGCG28650.039.930195
ACATGGG2129150.039.9113243
TGGGGTC37750.039.8622556
Produced by FastQC (version 0.11.5)