##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527837_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2054751
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.45357564006539	34.0	31.0	34.0	31.0	34.0
2	32.946397884707196	34.0	31.0	34.0	31.0	34.0
3	32.42595282834757	34.0	31.0	34.0	31.0	34.0
4	36.277424369181475	37.0	37.0	37.0	35.0	37.0
5	36.136746009613816	37.0	37.0	37.0	35.0	37.0
6	36.349525076274446	37.0	37.0	37.0	35.0	37.0
7	36.541290891207744	37.0	37.0	37.0	35.0	37.0
8	36.64352322982201	37.0	37.0	37.0	35.0	37.0
9	38.6393957224014	39.0	39.0	39.0	38.0	39.0
10	38.066865522878444	39.0	39.0	39.0	37.0	39.0
11	37.87792194772019	39.0	38.0	39.0	35.0	39.0
12	37.525234931142506	39.0	37.0	39.0	35.0	39.0
13	37.34605360941545	39.0	37.0	39.0	35.0	39.0
14	38.16597339531651	40.0	38.0	40.0	34.0	40.0
15	38.26691555327142	40.0	38.0	40.0	35.0	40.0
16	38.13817391985695	40.0	38.0	40.0	34.0	40.0
17	38.241992338731066	40.0	38.0	40.0	34.0	40.0
18	38.214691220493386	40.0	38.0	40.0	34.0	40.0
19	37.94665290344183	40.0	38.0	40.0	34.0	40.0
20	38.037276536183704	40.0	38.0	40.0	34.0	40.0
21	38.073165069636175	40.0	38.0	40.0	34.0	40.0
22	38.11855548433849	40.0	38.0	40.0	34.0	40.0
23	38.03219368186218	40.0	38.0	40.0	34.0	40.0
24	37.912766072385416	40.0	38.0	40.0	34.0	40.0
25	37.64199895753792	40.0	37.0	40.0	34.0	40.0
26	37.2718674914868	40.0	35.0	40.0	33.0	40.0
27	37.06139186694641	39.0	35.0	40.0	33.0	40.0
28	36.87400371139861	39.0	35.0	40.0	32.0	40.0
29	36.82829744333985	40.0	35.0	40.0	32.0	40.0
30	36.77179862669492	40.0	35.0	40.0	32.0	40.0
31	36.72282237604459	40.0	35.0	40.0	32.0	40.0
32	36.42391997862515	40.0	35.0	40.0	31.0	40.0
33	36.32932798183332	40.0	35.0	40.0	30.0	40.0
34	35.876901872781666	40.0	35.0	40.0	28.0	40.0
35	35.86638721674792	39.0	35.0	40.0	28.0	40.0
36	35.57286454660443	39.0	35.0	40.0	26.0	40.0
37	35.121109078423615	39.0	35.0	40.0	23.0	40.0
38	34.90948830296226	39.0	35.0	40.0	22.0	40.0
39	34.58780260965927	39.0	35.0	40.0	18.0	40.0
40	34.11264673919127	39.0	34.0	40.0	15.0	40.0
41	33.90844194746711	38.0	34.0	40.0	15.0	40.0
42	33.7683388400833	38.0	34.0	40.0	14.0	40.0
43	33.5834283570126	38.0	34.0	40.0	12.0	40.0
44	33.26491725761418	38.0	33.0	40.0	10.0	40.0
45	33.2845079525451	37.0	33.0	40.0	11.0	40.0
46	33.1905048348924	37.0	33.0	40.0	10.0	40.0
47	33.08659492074709	37.0	33.0	40.0	10.0	40.0
48	32.95935906589168	37.0	33.0	40.0	10.0	40.0
49	32.822728885397794	36.0	33.0	40.0	10.0	40.0
50	32.19227341901768	35.0	31.0	40.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	2.0
12	1.0
13	5.0
14	11.0
15	45.0
16	142.0
17	422.0
18	793.0
19	1611.0
20	2590.0
21	4177.0
22	6701.0
23	10035.0
24	14982.0
25	21574.0
26	29048.0
27	38153.0
28	49075.0
29	58422.0
30	60457.0
31	54459.0
32	53896.0
33	58930.0
34	93345.0
35	121122.0
36	172432.0
37	246308.0
38	560465.0
39	395547.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	100.0	0.0	0.0	0.0
2	100.0	0.0	0.0	0.0
3	0.0	100.0	0.0	0.0
4	0.0	0.0	0.0	100.0
5	0.0	100.0	0.0	0.0
6	0.0	0.0	100.0	0.0
7	99.27660334512551	0.3360991185793315	0.2580361318719397	0.12926140442321235
8	98.21803225792323	1.2327041086730217	0.35921627486736835	0.19004735853638713
9	95.3205522226294	2.530379593439789	1.0923951369290001	1.056673047001802
10	48.00435673227559	35.38263273749471	7.274166066837296	9.338844463392402
11	30.253787441884683	34.43083857849443	20.035761024085158	15.279612955535733
12	30.600982795482274	27.683110994957538	22.224736719923726	19.491169489636455
13	21.421330370443915	28.103307894727873	23.973951101617665	26.501410633210543
14	21.766871022328253	34.48948315391986	23.706716774927962	20.03692904882392
15	26.50905146170996	30.221910100055922	22.738375598795184	20.53066283943894
16	24.953923857440635	30.667365534802027	25.977502870177453	18.401207737579885
17	30.34956547046333	31.066099979997574	18.129641985817262	20.45469256372183
18	28.58127335136958	30.65884868774854	19.783954357486625	20.975923603395252
19	24.00602311423623	31.417675426365534	24.237292012511492	20.339009446886752
20	24.10953930670918	35.53909938479164	20.17704334977815	20.174317958721034
21	24.629066976971906	30.661817417292898	25.92951652049324	18.77959908524196
22	24.7151601337583	30.269020431186068	20.138498533398938	24.877320901656695
23	23.67075134651352	35.499532546766005	20.522097324688005	20.30761878203247
24	23.605439296537632	36.09493315735094	19.901778853009443	20.397848693101984
25	23.65683238504325	31.257996711037006	19.29659603523736	25.788574868682385
26	27.733676732606533	31.037970050872342	19.680584168106012	21.547769048415113
27	23.47101911618488	30.345185377692967	20.27681212954757	25.906983376574583
28	24.624248874924504	35.502160602428226	19.103239273274475	20.770351249372794
29	29.187332187695738	30.924866322002032	19.74618822426659	20.14161326603564
30	24.77025196727	34.266828438092986	19.831843371775946	21.13107622286107
31	29.60120228679777	30.496298578270554	19.72448243120456	20.17801670372712
32	27.264812135387693	30.864518377165894	22.458487670768868	19.412181816677542
33	29.668753050856285	30.744795841442592	19.959985419157846	19.62646568854328
34	24.9533885127687	31.908586490528535	21.145773867490515	21.99225112921225
35	25.10678909512637	35.88215798410611	20.494162066352565	18.516890854414964
36	28.1284204266113	32.31729781370103	20.04150381238408	19.51277794730359
37	25.792419616780816	31.74535503328627	22.744459060976244	19.717766288956668
38	29.04697454825427	30.917858173569453	20.16268637903084	19.872480899145444
39	26.710730399936537	28.978012420969744	20.090317512924923	24.220939666168796
40	26.00164204811191	29.65074600280034	22.59383253737314	21.753779411714607
41	27.649384280625732	29.275810061657104	20.96502203916679	22.109783618550374
42	27.233567473625758	28.117178188500695	22.135918172080217	22.513336165793323
43	25.284426190813384	30.083645171604733	22.345383941898554	22.28654469568332
44	25.20490317318254	28.043397959168775	21.73319297569389	25.01850589195479
45	24.629066976971906	31.255611993862033	21.801960432188622	22.313360596977443
46	24.824127108345486	29.26072307544807	23.930880189375745	21.984269626830695
47	26.787187352628127	29.595021489221807	21.901291202681005	21.716499955469057
48	28.205120717790138	28.926668000161577	21.254594838985355	21.61361644306293
49	27.340636408012454	28.772099393065144	21.930491821150106	21.956772377772293
50	23.61429681747326	30.81794339070768	22.603128067585804	22.964631724233254
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	168.5
6	337.0
7	373.5
8	410.0
9	807.0
10	1204.0
11	11608.5
12	22013.0
13	21730.5
14	21448.0
15	20873.0
16	20298.0
17	16858.0
18	13418.0
19	13665.5
20	13913.0
21	14203.0
22	14493.0
23	16526.0
24	18559.0
25	21794.5
26	25030.0
27	28929.0
28	32828.0
29	34474.5
30	36121.0
31	38501.5
32	40882.0
33	41654.5
34	42427.0
35	45182.5
36	47938.0
37	51060.0
38	54182.0
39	58313.5
40	62445.0
41	64766.0
42	67087.0
43	68444.5
44	69802.0
45	72241.0
46	74680.0
47	80639.0
48	86598.0
49	88871.5
50	91145.0
51	94475.5
52	97806.0
53	107036.0
54	116266.0
55	132714.5
56	149163.0
57	152555.0
58	155947.0
59	154986.0
60	154025.0
61	144957.0
62	135889.0
63	126733.0
64	117577.0
65	103727.5
66	89878.0
67	75372.5
68	60867.0
69	49614.5
70	38362.0
71	34238.5
72	30115.0
73	24292.5
74	18470.0
75	19039.0
76	19608.0
77	13802.0
78	7996.0
79	5606.5
80	3217.0
81	2428.5
82	1640.0
83	1077.0
84	514.0
85	310.0
86	106.0
87	69.0
88	32.0
89	21.0
90	10.0
91	6.0
92	2.0
93	2.5
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2054751.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.669801443155194
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.98868704123343	14.373635004290438
2	9.059517615693622	3.382787901115335
3	3.3682012905344734	1.8865094794457309
4	1.6578436047344405	1.2380644369678668
5	0.931978631003327	0.8699927995047858
6	0.5919857264591779	0.6631353582104902
7	0.4279447416934152	0.559275034924088
8	0.30942806799305084	0.46215684722955075
9	0.26076604331370684	0.4381605226605703
>10	4.036979937417102	20.16374073690586
>50	1.543669813876842	19.94723662019915
>100	0.7829612037217333	25.092994671232006
>500	0.027574658025701616	3.544539692833488
>1k	0.011135919587302574	4.1802352271588505
>5k	5.302818851096465E-4	0.7410837754543698
>10k+	7.954228276644697E-4	2.456451891867453
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGACATGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGGACATGGGA	23207	1.1294312546873075	No Hit
GGACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	15539	0.7562473506522202	No Hit
GGACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	10878	0.5294072128447681	No Hit
GGACATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	8019	0.39026626583951046	No Hit
GGACATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC	6952	0.33833783266196243	No Hit
GGACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	4750	0.23117156288036847	No Hit
GGACATGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC	4172	0.20304163375513626	No Hit
GGACATGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAA	4164	0.20265229217554828	Illumina Single End Adapter 2 (100% over 34bp)
GGACATGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	3907	0.19014469393128414	No Hit
GGACATGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3853	0.1875166382690652	No Hit
GGACATGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC	3466	0.16868223935649623	No Hit
GGACATGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC	3396	0.16527550053510134	No Hit
GGACATGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA	2727	0.13271681094205576	Illumina Single End Adapter 2 (97% over 34bp)
GGACATGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA	2703	0.1315487862032918	Illumina Single End Adapter 2 (97% over 34bp)
GGACATGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTT	2302	0.11203303952644382	No Hit
GGACATGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2206	0.10736094057138797	No Hit
GGACATGGGAAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCG	2170	0.10560890346324203	Illumina Single End Adapter 2 (100% over 23bp)
GGACATGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA	2111	0.10273750931378059	Illumina Single End Adapter 2 (97% over 34bp)
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.03241268650070008	0.0	0.0	0.0	0.0
8	0.1134444027524503	0.0	0.0	0.0	0.0
9	0.20328497224237876	0.0	0.0	0.0	0.0
10	0.461564442601561	0.0	0.0	0.0	0.0
11	0.6695215137989956	0.0	0.0	0.0	0.0
12	0.7686576135015873	0.0	0.0	0.0	0.0
13	0.8504680129125135	0.0	0.0	0.0	0.0
14	0.9529135160416031	0.0	0.0	0.0	0.0
15	1.0400286944744157	0.0	0.0	0.0	0.0
16	1.138726784899971	0.0	0.0	0.0	0.0
17	1.2322660993959853	0.0	0.0	0.0	0.0
18	1.315877203612506	0.0	0.0	0.0	0.0
19	1.4065451239590587	0.0	0.0	0.0	0.0
20	1.5100126487345669	0.0	0.0	0.0	0.0
21	1.609976099293783	0.0	0.0	0.0	0.0
22	1.7133949563718427	0.0	0.0	0.0	0.0
23	1.8424130223078126	0.0	0.0	0.0	0.0
24	1.9786825751636086	0.0	0.0	0.0	0.0
25	2.1017631820108615	0.0	0.0	0.0	0.0
26	2.2664060024791324	0.0	0.0	0.0	0.0
27	2.4051089402073536	0.0	0.0	0.0	0.0
28	2.6237729048434577	0.0	0.0	0.0	0.0
29	2.7201349457914854	0.0	0.0	0.0	0.0
30	2.8477416485014486	0.0	0.0	0.0	0.0
31	2.913978384728855	0.0	0.0	0.0	0.0
32	2.990386669722998	0.0	0.0	0.0	0.0
33	3.053362670221355	0.0	0.0	0.0	0.0
34	3.1183827140125495	0.0	0.0	0.0	0.0
35	3.257231654833116	0.0	0.0	0.0	0.0
36	3.3097927680774943	0.0	0.0	0.0	0.0
37	3.3662959648152015	0.0	0.0	0.0	0.0
38	3.411605591139754	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTAG	35	1.4468242E-7	44.0	14
TATATCG	20	7.8579324E-4	44.0	19
TACGGTT	20	7.8579324E-4	44.0	42
TAACGCG	30	2.5286936E-6	44.0	37
CGTTCGA	40	8.3182385E-9	44.0	21
ATGTACG	25	4.4440676E-5	44.0	32
CCGTATA	20	7.8579324E-4	44.0	12
TTACGGT	20	7.8579324E-4	44.0	41
CGTCATA	25	4.4440676E-5	44.0	26
CGATATT	25	4.4440676E-5	44.0	10
ATGGGAT	15675	0.0	41.445614	5
ATGGGGT	18515	0.0	41.029438	5
ATGGGAC	8820	0.0	40.8322	5
ATGGGAG	18535	0.0	40.545994	5
ATGGGGC	18340	0.0	40.485275	5
CATGGGG	103750	0.0	40.204338	4
GGACATG	224750	0.0	39.937706	1
ATGGGCG	2865	0.0	39.93019	5
ACATGGG	212915	0.0	39.911324	3
TGGGGTC	3775	0.0	39.862255	6
>>END_MODULE