FastQCFastQC Report
Fri 17 Jun 2016
SRR1527836_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527836_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3070577
Sequences flagged as poor quality0
Sequence length52
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACATGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGGACATGGGAAG304890.9929404147819775No Hit
GGACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA207280.675052278447992No Hit
GGACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA146330.4765553835647176No Hit
GGACATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA108370.3529304101476693No Hit
GGACATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA104420.3400663784037984No Hit
GGACATGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT63580.20706206032286442No Hit
GGACATGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG60170.19595665570347204No Hit
GGACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC58630.19094131168181094No Hit
GGACATGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA52480.17091250276413847No Hit
GGACATGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC52060.1695446816673218No Hit
GGACATGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC51240.16687417381163214No Hit
GGACATGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA49150.16006763549652067Illumina Single End Adapter 2 (100% over 34bp)
GGACATGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT35740.11639506190530315No Hit
GGACATGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGGGCATGGGAAG33840.11020729980065636No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATGCG206.31311E-446.00000420
ACGTACA206.31311E-446.00000426
ATAGTCG405.6170393E-946.00000417
ATACGTC351.0203257E-746.00000420
TCGTTAG650.046.014
ATGGGAT242500.043.1451535
CGAACGT800.043.12500438
ATGGGAG280050.042.4767045
TGGGATT88350.042.4595346
ATGGGAC136950.042.4395755
ATGGGGC281200.042.286635
ATGGGGT273100.042.016485
GGACATG3368500.041.637611
ACATGGG3192950.041.5648843
CATGGGG1541050.041.544924
GACATGG3328250.041.525432
ATGGGCT88750.041.4907045
ATGGGGG546200.041.09855
CATGGGA1164950.040.9220124
TGGGGTT60700.040.6952256
Produced by FastQC (version 0.11.5)