##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527836_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3070577
Sequences flagged as poor quality	0
Sequence length	52
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.07354578634569	33.0	31.0	34.0	30.0	34.0
2	32.63333536335353	34.0	31.0	34.0	31.0	34.0
3	32.031837664386856	33.0	31.0	34.0	30.0	34.0
4	36.11641460220669	37.0	35.0	37.0	35.0	37.0
5	35.83331569278347	37.0	35.0	37.0	35.0	37.0
6	36.124459669957794	37.0	35.0	37.0	35.0	37.0
7	36.44080119143731	37.0	36.0	37.0	35.0	37.0
8	36.55544479099531	37.0	37.0	37.0	35.0	37.0
9	38.55576981134165	39.0	39.0	39.0	37.0	39.0
10	37.799143288053024	39.0	38.0	39.0	35.0	39.0
11	37.53279725602061	39.0	37.0	39.0	35.0	39.0
12	37.19174637209879	39.0	37.0	39.0	34.0	39.0
13	37.023466599274336	39.0	37.0	39.0	33.0	39.0
14	37.83816885230365	40.0	38.0	40.0	33.0	40.0
15	37.955919034109876	40.0	38.0	40.0	33.0	40.0
16	37.7913268418281	40.0	37.0	40.0	33.0	40.0
17	37.90162272432836	40.0	38.0	40.0	33.0	40.0
18	37.906853988680304	40.0	38.0	40.0	33.0	40.0
19	37.59059877019857	40.0	37.0	40.0	33.0	40.0
20	37.79199479446371	40.0	37.0	40.0	33.0	40.0
21	37.824714052114636	40.0	37.0	40.0	33.0	40.0
22	37.8640170886449	40.0	38.0	40.0	33.0	40.0
23	37.80962665974506	40.0	37.0	40.0	33.0	40.0
24	37.672631886450006	40.0	37.0	40.0	33.0	40.0
25	37.44139033152401	40.0	37.0	40.0	33.0	40.0
26	37.00536544108811	39.0	35.0	40.0	32.0	40.0
27	36.8126280500375	39.0	35.0	40.0	32.0	40.0
28	36.67060327749475	39.0	35.0	40.0	31.0	40.0
29	36.5289777784436	39.0	35.0	40.0	31.0	40.0
30	36.20931212602713	39.0	35.0	40.0	30.0	40.0
31	36.09495837427298	39.0	35.0	40.0	30.0	40.0
32	35.572322400643266	38.0	35.0	40.0	27.0	40.0
33	35.54597849199027	38.0	35.0	40.0	27.0	40.0
34	35.22608649774945	38.0	35.0	40.0	25.0	40.0
35	35.15243486810459	38.0	35.0	40.0	25.0	40.0
36	34.89856922656556	38.0	34.0	40.0	24.0	40.0
37	34.392973047085285	38.0	34.0	40.0	21.0	40.0
38	34.25322406831029	38.0	33.0	40.0	21.0	40.0
39	33.933702688452364	38.0	33.0	40.0	18.0	40.0
40	33.454269669837295	38.0	33.0	40.0	15.0	40.0
41	33.240522546739584	37.0	33.0	40.0	15.0	40.0
42	33.14743417930897	37.0	33.0	40.0	14.0	40.0
43	32.871795105610445	37.0	32.0	40.0	10.0	40.0
44	32.67486697125654	37.0	32.0	40.0	10.0	40.0
45	32.66263962766607	36.0	32.0	40.0	10.0	40.0
46	32.52760539794312	36.0	31.0	40.0	10.0	40.0
47	32.37632861836717	36.0	31.0	40.0	10.0	40.0
48	32.24874868795018	35.0	31.0	40.0	10.0	40.0
49	32.41565086952713	35.0	31.0	40.0	10.0	40.0
50	32.1990599812348	35.0	31.0	40.0	10.0	40.0
51	32.17097991680391	35.0	31.0	40.0	10.0	40.0
52	31.686843221974243	35.0	31.0	39.0	9.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	2.0
13	5.0
14	27.0
15	73.0
16	276.0
17	678.0
18	1551.0
19	3087.0
20	5497.0
21	9106.0
22	14689.0
23	22821.0
24	33091.0
25	44679.0
26	58733.0
27	73883.0
28	85905.0
29	88868.0
30	82725.0
31	82915.0
32	93703.0
33	112930.0
34	168419.0
35	198896.0
36	300840.0
37	406722.0
38	800704.0
39	379750.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	100.0	0.0	0.0	0.0
2	100.0	0.0	0.0	0.0
3	0.0	100.0	0.0	0.0
4	0.0	0.0	0.0	100.0
5	0.0	100.0	0.0	0.0
6	0.0	0.0	100.0	0.0
7	99.3227657212309	0.3217961966106045	0.2352977958214368	0.12014028633706303
8	98.27582894029364	1.191013936468618	0.3506507083196415	0.18250641491810823
9	95.28573945548345	2.5539499579395013	1.0601264843708527	1.1001841022061978
10	47.60730637922449	35.93637287063637	7.19802825332177	9.258292496817374
11	30.082977889823315	34.53611487352377	19.992529091437863	15.388378145215054
12	31.022149908632805	26.905464347580278	22.357361499157978	19.71502424462894
13	21.552789589709036	27.259274071290186	24.008744936212313	27.179191402788465
14	21.801309656133032	34.14260577083721	23.720786028163438	20.335298544866323
15	27.146689368154586	29.333574764612646	22.90377346016726	20.615962407065513
16	25.16041773256297	29.731317599265545	26.55784238597501	18.550422282196475
17	30.937149597616344	30.21249752082426	18.227844473530546	20.62250840802885
18	29.33152303296742	29.615183074712014	19.988067389288723	21.06522650303184
19	24.172753199154425	30.390574800762206	24.939677461271938	20.496994538811435
20	24.201770546708325	35.09682382171169	20.325430692667858	20.375974938912133
21	24.68584894630553	29.78922202569745	26.450240459692104	19.074688568304914
22	24.72222647404706	29.554705841931337	20.182949328416125	25.54011835560548
23	23.907591309385825	35.0992337922156	20.605345509980697	20.387829388417877
24	23.631942791208296	35.71133373304106	20.08720185163896	20.56952162411169
25	23.866328706298525	30.270401947256165	19.350043981961697	26.51322536448361
26	28.35017653033941	30.063339886933306	19.72896299294888	21.857520589778403
27	23.57794642505301	29.443716930075357	20.437233783748134	26.541102861123495
28	24.862916644005345	35.132745409087605	19.122301769341725	20.882036177565325
29	29.83579307732716	30.117727059116252	19.761465027582766	20.285014835973826
30	24.818136786669083	34.04317820396622	19.815005453372443	21.323679555992246
31	30.227055045354668	29.65452421482998	19.767196849321806	20.351223890493543
32	27.67763192390225	30.22223510434684	22.67824581503737	19.421887156713545
33	30.34292903255642	29.936881569815704	20.012851004876282	19.70733839275159
34	25.082191392692643	31.2291794017867	21.116552361331436	22.57207684418922
35	25.346767073419752	35.596990402780975	20.431404260502177	18.624838263297093
36	28.76980450254138	31.618259369493096	19.967973446033106	19.64396268193242
37	26.076662464416295	31.185278858012676	22.973695171949768	19.764363505621258
38	29.489604071156663	30.34732560036762	20.179562342843056	19.983507985632667
39	26.666193357144273	28.47129383174563	20.121397379059374	24.74111543205072
40	25.94235545957649	29.317844821999252	22.84365446624527	21.89614525217899
41	28.011184868511684	28.901213029342692	20.962183980404987	22.125418121740637
42	27.76562841446412	27.690886761673784	22.016318105685023	22.52716671817707
43	25.45772341810676	29.80794814785625	22.376869233372098	22.35745920066489
44	25.126743279846103	27.723063124617948	21.736403288372188	25.41379030716377
45	24.61426630890546	31.03989901572245	21.878591548103174	22.46724312726891
46	24.784429766783248	28.885548221067246	24.222385564667487	22.107636447482022
47	27.33303870901137	28.959801366323006	21.986616847582717	21.720543077082908
48	28.922414256343355	28.265469323843696	21.21852016738222	21.593596252430732
49	27.837048216019333	28.244593768532756	21.98235054844741	21.9360074670005
50	23.659331780313604	30.521364551353052	22.62506362810638	23.19424004022697
51	22.91233862560685	31.444513523028405	22.95080045216257	22.69234739920217
52	25.78906179522611	28.8890654753162	22.68635504011135	22.635517689346337
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	245.5
6	491.0
7	525.5
8	560.0
9	1081.0
10	1602.0
11	15535.0
12	29468.0
13	29005.0
14	27702.0
15	26862.0
16	21759.0
17	16656.0
18	17090.0
19	17524.0
20	17011.5
21	16499.0
22	18072.0
23	19645.0
24	23497.5
25	27350.0
26	32040.5
27	36731.0
28	40950.5
29	45170.0
30	48525.5
31	51881.0
32	55376.5
33	58872.0
34	63028.0
35	67184.0
36	70306.5
37	73429.0
38	79985.0
39	89292.5
40	92044.0
41	96488.0
42	100932.0
43	100498.0
44	100064.0
45	108883.0
46	117702.0
47	120005.0
48	122308.0
49	123097.5
50	123887.0
51	132763.5
52	141640.0
53	153888.5
54	166137.0
55	190589.0
56	215041.0
57	220367.0
58	225693.0
59	230811.0
60	235929.0
61	216290.5
62	196652.0
63	187283.5
64	158907.0
65	139899.0
66	121124.5
67	102350.0
68	84333.5
69	66317.0
70	55858.5
71	45400.0
72	41602.5
73	37805.0
74	34449.0
75	31093.0
76	23679.0
77	16265.0
78	11003.0
79	5741.0
80	4345.5
81	2950.0
82	2154.5
83	1359.0
84	864.0
85	369.0
86	206.0
87	43.0
88	33.0
89	15.5
90	8.0
91	5.0
92	2.0
93	2.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	3070577.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.482111498202617
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.7551933923876	14.555622653406175
2	8.585328108484019	3.1734998269930923
3	3.482068688618123	1.9306794524232098
4	1.7687548531874937	1.3076129763839304
5	0.9822491761775213	0.9077019396565307
6	0.604779442094177	0.6706560648363211
7	0.42754900417886654	0.5531405856325515
8	0.31443184658027784	0.46490915576659475
9	0.226752870328277	0.37717846487502255
>10	2.4060991020778686	11.209413869711325
>50	1.3825188806319915	18.389201377097955
>100	1.0118465160597365	33.41830540875777
>500	0.036011233385881296	4.519862190979722
>1k	0.014475103615893464	4.576304596910231
>5k	0.0010591539231141558	1.1032695947665265
>10k+	8.826282692617964E-4	2.842641841803072
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGACATGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGGACATGGGAAG	30489	0.9929404147819775	No Hit
GGACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	20728	0.675052278447992	No Hit
GGACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	14633	0.4765553835647176	No Hit
GGACATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	10837	0.3529304101476693	No Hit
GGACATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	10442	0.3400663784037984	No Hit
GGACATGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	6358	0.20706206032286442	No Hit
GGACATGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	6017	0.19595665570347204	No Hit
GGACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	5863	0.19094131168181094	No Hit
GGACATGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5248	0.17091250276413847	No Hit
GGACATGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	5206	0.1695446816673218	No Hit
GGACATGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	5124	0.16687417381163214	No Hit
GGACATGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA	4915	0.16006763549652067	Illumina Single End Adapter 2 (100% over 34bp)
GGACATGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT	3574	0.11639506190530315	No Hit
GGACATGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGGGCATGGGAAG	3384	0.11020729980065636	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.028040332484741467	0.0	0.0	0.0	0.0
8	0.10203294038872825	0.0	0.0	0.0	0.0
9	0.18644704236369908	0.0	0.0	0.0	0.0
10	0.4295283915694021	0.0	0.0	0.0	0.0
11	0.6162034041159039	0.0	0.0	0.0	0.0
12	0.7064144621678596	0.0	0.0	0.0	0.0
13	0.779364920664748	0.0	0.0	0.0	0.0
14	0.8743959197245339	0.0	0.0	0.0	0.0
15	0.9579632753062373	0.0	0.0	0.0	0.0
16	1.0519846921278966	0.0	0.0	0.0	0.0
17	1.1412513022796693	0.0	0.0	3.256716897182517E-5	0.0
18	1.21674199995636	0.0	0.0	3.256716897182517E-5	0.0
19	1.3031101320696403	0.0	0.0	3.256716897182517E-5	0.0
20	1.3927675482490751	0.0	0.0	3.256716897182517E-5	0.0
21	1.4780283966173133	0.0	0.0	3.256716897182517E-5	0.0
22	1.5704865893283249	0.0	0.0	3.256716897182517E-5	0.0
23	1.6735942462931235	0.0	0.0	3.256716897182517E-5	0.0
24	1.790119576874314	0.0	0.0	3.256716897182517E-5	0.0
25	1.8874302777621275	0.0	0.0	3.256716897182517E-5	0.0
26	2.0280553133824686	0.0	0.0	3.256716897182517E-5	0.0
27	2.135624672496407	0.0	0.0	3.256716897182517E-5	0.0
28	2.3128552060410796	0.0	0.0	3.256716897182517E-5	0.0
29	2.3821255744441516	0.0	0.0	3.256716897182517E-5	0.0
30	2.4822370518635424	0.0	0.0	3.256716897182517E-5	0.0
31	2.5336931788390262	0.0	0.0	3.256716897182517E-5	0.0
32	2.5978830688824934	0.0	0.0	3.256716897182517E-5	0.0
33	2.6455288370882735	0.0	0.0	3.256716897182517E-5	0.0
34	2.700176546622996	0.0	0.0	3.256716897182517E-5	0.0
35	2.819470086566792	0.0	0.0	3.256716897182517E-5	0.0
36	2.8647710186066004	0.0	0.0	3.256716897182517E-5	0.0
37	2.9136217720643383	0.0	0.0	3.256716897182517E-5	0.0
38	2.9573269128245276	0.0	0.0	3.256716897182517E-5	0.0
39	2.9944860526213803	0.0	0.0	3.256716897182517E-5	0.0
40	3.031189252052627	0.0	0.0	3.256716897182517E-5	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTATGCG	20	6.31311E-4	46.000004	20
ACGTACA	20	6.31311E-4	46.000004	26
ATAGTCG	40	5.6170393E-9	46.000004	17
ATACGTC	35	1.0203257E-7	46.000004	20
TCGTTAG	65	0.0	46.0	14
ATGGGAT	24250	0.0	43.145153	5
CGAACGT	80	0.0	43.125004	38
ATGGGAG	28005	0.0	42.476704	5
TGGGATT	8835	0.0	42.459534	6
ATGGGAC	13695	0.0	42.439575	5
ATGGGGC	28120	0.0	42.28663	5
ATGGGGT	27310	0.0	42.01648	5
GGACATG	336850	0.0	41.63761	1
ACATGGG	319295	0.0	41.564884	3
CATGGGG	154105	0.0	41.54492	4
GACATGG	332825	0.0	41.52543	2
ATGGGCT	8875	0.0	41.490704	5
ATGGGGG	54620	0.0	41.0985	5
CATGGGA	116495	0.0	40.922012	4
TGGGGTT	6070	0.0	40.695225	6
>>END_MODULE