FastQCFastQC Report
Fri 17 Jun 2016
SRR1527835_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527835_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3086989
Sequences flagged as poor quality0
Sequence length52
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACATGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGGACATGGGAAG283740.9191480760054539No Hit
GGACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA195360.6328496797364681No Hit
GGACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA138770.44953189013631084No Hit
GGACATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA102930.3334317031903904No Hit
GGACATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA101220.32789232485117376No Hit
GGACATGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT61090.19789510101914845No Hit
GGACATGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG57070.18487270281818302No Hit
GGACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC55160.1786854439714557No Hit
GGACATGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA49980.16190533882692812No Hit
GGACATGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC49940.16177576272542596No Hit
GGACATGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC48530.1572082051474754No Hit
GGACATGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA47240.15302937587403131Illumina Single End Adapter 2 (100% over 34bp)
GGACATGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGGACATGGGGAG38950.12617472883771208No Hit
GGACATGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT34420.11150023534259436No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTAG600.046.014
TACGACG206.3131173E-446.021
CGTACCG453.110472E-1046.032
CGATAAC206.3131173E-446.040
ACGGTAC206.3131173E-446.032
CGAACGT900.043.44444738
ATGGGAT237950.043.1582265
ATGGGAC141250.042.661955
ATGGGAG282100.042.3963135
ATGGGGC279300.042.335485
TGGGATT84000.042.303576
ATGGGGT276450.042.2145085
GGACATG3387600.041.626231
TAACGGG1050.041.61904529
ACATGGG3209100.041.6108553
ATGGGCT91350.041.593875
CATGGGG1557900.041.5370034
GACATGG3348900.041.520742
ATGGGGG553750.041.2110145
CATGGGA1164350.041.0576744
Produced by FastQC (version 0.11.5)