##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527835_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3086989
Sequences flagged as poor quality	0
Sequence length	52
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.032390785973	33.0	31.0	34.0	30.0	34.0
2	32.6181622286312	34.0	31.0	34.0	31.0	34.0
3	32.03669789558693	33.0	31.0	34.0	30.0	34.0
4	36.10774447204056	37.0	35.0	37.0	35.0	37.0
5	35.860933096943334	37.0	35.0	37.0	35.0	37.0
6	36.07064618629998	37.0	35.0	37.0	35.0	37.0
7	36.40316470191504	37.0	36.0	37.0	35.0	37.0
8	36.53142787356871	37.0	37.0	37.0	35.0	37.0
9	38.514302124173426	39.0	39.0	39.0	37.0	39.0
10	37.71938643124417	39.0	38.0	39.0	35.0	39.0
11	37.50113460073878	39.0	37.0	39.0	35.0	39.0
12	37.14132023146179	39.0	37.0	39.0	34.0	39.0
13	36.82076450547767	39.0	37.0	39.0	33.0	39.0
14	37.771485418315386	40.0	38.0	40.0	33.0	40.0
15	37.90635373174313	40.0	38.0	40.0	33.0	40.0
16	37.76680610134989	40.0	37.0	40.0	33.0	40.0
17	37.91911794956185	40.0	38.0	40.0	33.0	40.0
18	37.9352226392773	40.0	38.0	40.0	33.0	40.0
19	37.56998032710839	40.0	37.0	40.0	33.0	40.0
20	37.78760889656555	40.0	37.0	40.0	33.0	40.0
21	37.87130015688427	40.0	38.0	40.0	33.0	40.0
22	37.81018040556672	40.0	37.0	40.0	33.0	40.0
23	37.77444526041395	40.0	37.0	40.0	33.0	40.0
24	37.68540023952142	40.0	37.0	40.0	33.0	40.0
25	37.426149558679995	40.0	37.0	40.0	33.0	40.0
26	36.94884594664899	39.0	35.0	40.0	32.0	40.0
27	36.757153005728235	39.0	35.0	40.0	31.0	40.0
28	36.63526497826847	39.0	35.0	40.0	31.0	40.0
29	36.49554047649668	39.0	35.0	40.0	31.0	40.0
30	36.16537344318363	39.0	35.0	40.0	30.0	40.0
31	36.07053313115142	39.0	35.0	40.0	30.0	40.0
32	35.556231006977995	38.0	35.0	40.0	27.0	40.0
33	35.38134149490005	38.0	35.0	40.0	26.0	40.0
34	35.159332605331606	38.0	35.0	40.0	25.0	40.0
35	35.15226617263618	38.0	34.0	40.0	25.0	40.0
36	34.76502410601398	38.0	34.0	40.0	24.0	40.0
37	34.33096878544109	38.0	34.0	40.0	21.0	40.0
38	33.944978747899654	38.0	33.0	40.0	18.0	40.0
39	33.78591890026171	38.0	33.0	40.0	18.0	40.0
40	33.37771757528129	38.0	33.0	40.0	15.0	40.0
41	32.988293771050046	37.0	32.0	40.0	12.0	40.0
42	33.02897969510096	37.0	32.0	40.0	13.0	40.0
43	32.861866368814404	37.0	32.0	40.0	11.0	40.0
44	32.65964180630381	37.0	32.0	40.0	10.0	40.0
45	32.64362134105434	36.0	32.0	40.0	10.0	40.0
46	32.34966920840988	36.0	31.0	40.0	10.0	40.0
47	32.126816778420654	35.0	31.0	40.0	10.0	40.0
48	32.14368305167268	35.0	31.0	40.0	10.0	40.0
49	32.063461515411944	35.0	31.0	39.0	10.0	40.0
50	31.613018705282073	35.0	30.0	39.0	10.0	40.0
51	31.818897637795274	35.0	31.0	39.0	10.0	40.0
52	31.517937057760815	35.0	30.0	39.0	9.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	4.0
13	6.0
14	15.0
15	74.0
16	243.0
17	734.0
18	1555.0
19	3100.0
20	5531.0
21	9319.0
22	14988.0
23	23556.0
24	33971.0
25	45506.0
26	59943.0
27	73956.0
28	87532.0
29	90192.0
30	84137.0
31	85799.0
32	97556.0
33	118647.0
34	174997.0
35	205457.0
36	312412.0
37	434082.0
38	794584.0
39	329092.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	100.0	0.0	0.0	0.0
2	100.0	0.0	0.0	0.0
3	0.0	100.0	0.0	0.0
4	0.0	0.0	0.0	100.0
5	0.0	100.0	0.0	0.0
6	0.0	0.0	100.0	0.0
7	99.30903543873983	0.32539798489725746	0.24301997836726985	0.12254659799565207
8	98.28499550856839	1.1883424268761567	0.3444780658434481	0.18218399871201355
9	95.24688944469838	2.5572167571701745	1.0843576054206867	1.1115361927107612
10	47.601271011979634	35.94424858656769	7.210229774061391	9.244250627391287
11	30.09712700628347	34.53028177295092	19.91876226316323	15.453828957602376
12	31.0376227450114	26.86572579299764	22.311061037146555	19.785590424844404
13	21.81264656271856	27.163718432427196	24.002223525901776	27.021411478952466
14	21.813035291023063	34.0487121917182	23.77154567120259	20.36670684605614
15	27.11354008712049	29.30240438174545	22.91307160472551	20.67098392640855
16	25.172133752339253	29.649506363644313	26.627240978182947	18.551118905833484
17	31.018315905887583	30.10574381703336	18.26326559634647	20.612674680732585
18	29.340888483891586	29.558576334415186	20.032173746002982	21.068361435690246
19	24.223701477394314	30.321034509679173	24.938929163660774	20.516334849265743
20	24.249681485745494	35.00410918211889	20.340273321349702	20.405936010785915
21	24.608218558601926	29.756989739840346	26.4899874926668	19.14480420889093
22	24.95315014080063	29.416139804838952	20.1300037026371	25.500706351723313
23	23.989330703802313	35.000578233352954	20.661395294897392	20.34869576794734
24	23.72748979669186	35.58765515523379	20.091292842313337	20.593562205761017
25	23.93646365438944	30.14691662328567	19.32886706107472	26.587752661250168
26	28.4591231131695	29.94824406565751	19.728836092386466	21.86379672878653
27	23.61637828965377	29.324918229381446	20.464925530994766	26.593777949970022
28	24.900671819692263	34.98574176973096	19.144059146307292	20.969527264269487
29	29.94568493765284	30.039983945521026	19.76913425995363	20.245196856872504
30	24.886871964882285	33.97229468585732	19.83544482989735	21.305388519363042
31	30.26502523980487	29.547594759812878	19.745583803505617	20.441796196876634
32	27.72063651668341	30.08705246439168	22.738467807951373	19.453843210973538
33	30.319123262182018	29.785917604500696	20.162883638393268	19.73207549492402
34	25.17838579923673	31.058678861505502	21.188057359452852	22.574877979804917
35	25.472264397443595	35.490278714954925	20.454332684696965	18.583124202904514
36	29.00052445927083	31.435874892978237	20.01630067356897	19.54729997418196
37	26.136374311667453	31.120000751541387	22.960528851900673	19.783096084890488
38	29.584783100943994	30.22226512630916	20.10658282229059	20.086368950456254
39	26.722025896431767	28.48701436901784	20.05569180842562	24.735267926124777
40	25.979943563128995	29.22200241076337	22.848445524101315	21.949608502006324
41	28.204344103590913	28.8113433510777	20.975487764938585	22.008824780392803
42	27.792421676915595	27.679042588101222	22.03010765506453	22.498428079918654
43	25.440453464524815	29.796478056773125	22.336879075370856	22.426189403331207
44	25.154219856306582	27.68267071894328	21.73833466850708	25.424774756243057
45	24.609870653896078	31.02913551036301	21.892206289040875	22.468787546700035
46	24.964520443707443	28.785007008447387	24.25583635056685	21.994636197278318
47	27.549984791005087	28.90661417970715	21.9194496643817	21.623951364906063
48	28.89806215700801	28.250894318055554	21.244260993479408	21.60678253145703
49	28.03388674206484	28.104440929332757	21.872024811231917	21.989647517370486
50	23.896942943431284	30.328614711617046	22.566779473461033	23.207662871490633
51	22.935812210539137	31.40374001980571	22.955637354068966	22.70481041558619
52	25.794779314082426	28.83026146189701	22.707920242022244	22.667038981998317
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	235.0
6	470.0
7	509.5
8	549.0
9	1092.0
10	1635.0
11	14937.0
12	28239.0
13	28328.5
14	27438.0
15	26458.0
16	21782.0
17	17106.0
18	17206.5
19	17307.0
20	16763.5
21	16220.0
22	17775.0
23	19330.0
24	23142.5
25	26955.0
26	31368.5
27	35782.0
28	40412.5
29	45043.0
30	48518.5
31	51994.0
32	55352.5
33	58711.0
34	62815.0
35	66919.0
36	70329.0
37	73739.0
38	80867.5
39	90405.0
40	92814.0
41	97243.0
42	101672.0
43	102031.5
44	102391.0
45	110118.5
46	117846.0
47	120468.5
48	123091.0
49	124199.0
50	125307.0
51	133682.0
52	142057.0
53	154813.0
54	167569.0
55	191097.0
56	214625.0
57	221613.0
58	228601.0
59	232881.5
60	237162.0
61	217696.0
62	198230.0
63	188635.0
64	159847.5
65	140655.0
66	121664.0
67	102673.0
68	85008.5
69	67344.0
70	56947.5
71	46551.0
72	42430.0
73	38309.0
74	34832.0
75	31355.0
76	23826.5
77	16298.0
78	11068.0
79	5838.0
80	4391.5
81	2945.0
82	2098.5
83	1252.0
84	831.0
85	410.0
86	230.0
87	50.0
88	34.5
89	14.0
90	9.0
91	6.0
92	3.0
93	2.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	3086989.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.397471822915655
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.9116419196185	15.306863506358098
2	8.656976089771858	3.358468995460083
3	3.4856375506987716	2.0283766852372853
4	1.7029475772452098	1.3213151058206576
5	0.9794817208469265	0.9499734540594598
6	0.5942038639202392	0.6915631624476268
7	0.4074734050454589	0.553276772506975
8	0.29176558692264665	0.45276118009827904
9	0.23925032040010102	0.41767662137260525
>10	2.4186344985204573	11.929020805613883
>50	1.3387111812795771	18.631093773835296
>100	0.9241087130253465	31.761589182325455
>500	0.03387871617480483	4.390478746363191
>1k	0.013898960481971211	4.853187802984492
>5k	5.212110180739204E-4	0.5840985702374658
>10k+	8.686850301232007E-4	2.7702556352792618
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGACATGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGGACATGGGAAG	28374	0.9191480760054539	No Hit
GGACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	19536	0.6328496797364681	No Hit
GGACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	13877	0.44953189013631084	No Hit
GGACATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	10293	0.3334317031903904	No Hit
GGACATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	10122	0.32789232485117376	No Hit
GGACATGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	6109	0.19789510101914845	No Hit
GGACATGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	5707	0.18487270281818302	No Hit
GGACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	5516	0.1786854439714557	No Hit
GGACATGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4998	0.16190533882692812	No Hit
GGACATGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	4994	0.16177576272542596	No Hit
GGACATGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	4853	0.1572082051474754	No Hit
GGACATGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA	4724	0.15302937587403131	Illumina Single End Adapter 2 (100% over 34bp)
GGACATGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGGACATGGGGAG	3895	0.12617472883771208	No Hit
GGACATGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT	3442	0.11150023534259436	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.029672927243990826	0.0	0.0	0.0	0.0
8	0.1000651443850302	0.0	0.0	0.0	0.0
9	0.18007838706260373	0.0	0.0	0.0	0.0
10	0.41866038395342514	0.0	0.0	0.0	0.0
11	0.600261290208679	0.0	0.0	0.0	0.0
12	0.6851012426672074	0.0	0.0	0.0	0.0
13	0.7582793459905429	0.0	0.0	0.0	0.0
14	0.8536797507214959	0.0	0.0	0.0	0.0
15	0.935539452845475	0.0	0.0	0.0	0.0
16	1.0287370638508917	0.0	0.0	0.0	0.0
17	1.1138037744870488	0.0	0.0	0.0	0.0
18	1.1918409816167146	0.0	0.0	0.0	0.0
19	1.2748344746288374	0.0	0.0	0.0	0.0
20	1.3615856745845223	0.0	0.0	3.2394025375535835E-5	0.0
21	1.4460045047131687	0.0	0.0	3.2394025375535835E-5	0.0
22	1.5369669279676734	0.0	0.0	3.2394025375535835E-5	0.0
23	1.6435108774278107	0.0	0.0	3.2394025375535835E-5	0.0
24	1.7560153275570467	0.0	0.0	3.2394025375535835E-5	0.0
25	1.8530678275821522	0.0	0.0	3.2394025375535835E-5	0.0
26	1.9896086445400356	0.0	0.0	3.2394025375535835E-5	0.0
27	2.096055411924046	0.0	0.0	3.2394025375535835E-5	0.0
28	2.2733803068297296	0.0	0.0	3.2394025375535835E-5	0.0
29	2.344873920833537	0.0	0.0	3.2394025375535835E-5	0.0
30	2.4408898120466254	0.0	0.0	3.2394025375535835E-5	0.0
31	2.4897076082875578	0.0	0.0	3.2394025375535835E-5	0.0
32	2.5529731398459794	0.0	0.0	3.2394025375535835E-5	0.0
33	2.6026007867213004	0.0	0.0	3.2394025375535835E-5	0.0
34	2.6568283851999475	0.0	0.0	3.2394025375535835E-5	0.0
35	2.773511664602627	0.0	0.0	3.2394025375535835E-5	0.0
36	2.816109807971457	0.0	0.0	3.2394025375535835E-5	0.0
37	2.8651219683646425	0.0	0.0	3.2394025375535835E-5	0.0
38	2.9071046252513373	0.0	0.0	3.2394025375535835E-5	0.0
39	2.9445521185854564	0.0	0.0	3.2394025375535835E-5	0.0
40	2.9803799106507993	0.0	0.0	3.2394025375535835E-5	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTAG	60	0.0	46.0	14
TACGACG	20	6.3131173E-4	46.0	21
CGTACCG	45	3.110472E-10	46.0	32
CGATAAC	20	6.3131173E-4	46.0	40
ACGGTAC	20	6.3131173E-4	46.0	32
CGAACGT	90	0.0	43.444447	38
ATGGGAT	23795	0.0	43.158226	5
ATGGGAC	14125	0.0	42.66195	5
ATGGGAG	28210	0.0	42.396313	5
ATGGGGC	27930	0.0	42.33548	5
TGGGATT	8400	0.0	42.30357	6
ATGGGGT	27645	0.0	42.214508	5
GGACATG	338760	0.0	41.62623	1
TAACGGG	105	0.0	41.619045	29
ACATGGG	320910	0.0	41.610855	3
ATGGGCT	9135	0.0	41.59387	5
CATGGGG	155790	0.0	41.537003	4
GACATGG	334890	0.0	41.52074	2
ATGGGGG	55375	0.0	41.211014	5
CATGGGA	116435	0.0	41.057674	4
>>END_MODULE