FastQCFastQC Report
Fri 17 Jun 2016
SRR1527834_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527834_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3702889
Sequences flagged as poor quality0
Sequence length50
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCACCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC319340.8624077038226099No Hit
CGCACCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC204240.5515693287052352No Hit
CGCACCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT158640.42842224004014157No Hit
CGCACCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG151790.40992317079988083No Hit
CGCACCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT141040.38089178476589497No Hit
CGCACCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT137670.3717907828184966No Hit
CGCACCGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC128940.3482145967648504No Hit
CGCACCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGT126590.3418682007481186No Hit
CGCACCGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG97490.26328091390263114No Hit
CGCACCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC91850.24804956346247484No Hit
CGCACCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC76300.20605532599005805No Hit
CGCACCGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGC61610.1663835994003601No Hit
CGCACCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA60090.16227869644485698No Hit
CGCACCGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCT59810.1615225301109485No Hit
CGCACCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTT54940.14837063708904047No Hit
CGCACCGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCC52620.14210525889379888No Hit
CGCACCGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCA46020.12428133816595636No Hit
CGCACCGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCG45980.12417331440396943No Hit
CGCACCGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG45680.1233631361890675No Hit
CGCACCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG45510.12290403520062308No Hit
CGCACCGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAA45200.12206685104522441No Hit
CGCACCGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTC45040.12163475599727672No Hit
CGCACCGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGC44200.11936625699555131No Hit
CGCACCGGGGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAA44040.11893416194760362No Hit
CGCACCGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGA41590.1123177065259045No Hit
CGCACCGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATC41530.11215567088292411No Hit
CGCACCGGGGTCTCTCGGGTGGAGTCTTCTGACTGCTGGTGGAGCAGGTC41000.11072435603659736No Hit
CGCACCGGGGGGGCTGGCGAGATGGCTCAGCGGGTAAGAGCACCCGATTG38230.10324371051900286No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCACCG3779450.042.7841
GCACCGG3753600.042.7258072
CACCGGG3556650.042.699173
ACCGGAT32300.042.637774
ACCGGGG1842150.042.602724
ACCGGGA1223500.042.5237434
ACCGGGT205150.042.3806954
CCGGGAT340450.042.2681735
ACCGGGC300200.042.1825454
CGGGATA32300.042.024776
CGGGATC103500.041.916916
CCGGGGG758800.041.9037975
CACCGGA109650.041.752853
CCGGGAC223150.041.7521865
CCGGGGT316200.041.5439575
CCGGGGA454300.041.5060545
CGGGGGG344050.041.4997836
CGGGATT113250.041.0472376
CGGGAGT139950.040.887466
CGGGGGA117200.040.79016
Produced by FastQC (version 0.11.5)