##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527834_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3702889
Sequences flagged as poor quality	0
Sequence length	50
%GC	61
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.69384418490535	34.0	31.0	34.0	31.0	34.0
2	33.00337115155221	34.0	31.0	34.0	31.0	34.0
3	33.16734177016919	34.0	34.0	34.0	31.0	34.0
4	36.51642379774279	37.0	37.0	37.0	35.0	37.0
5	36.483271305189	37.0	37.0	37.0	35.0	37.0
6	36.52220171871207	37.0	37.0	37.0	35.0	37.0
7	36.525115389632255	37.0	37.0	37.0	35.0	37.0
8	36.6750353575276	37.0	37.0	37.0	35.0	37.0
9	38.705526414645426	39.0	39.0	39.0	39.0	39.0
10	38.27042452528283	39.0	39.0	39.0	37.0	39.0
11	38.10019392965871	39.0	39.0	39.0	37.0	39.0
12	37.627295606214496	39.0	37.0	39.0	35.0	39.0
13	37.53175912105386	39.0	37.0	39.0	35.0	39.0
14	38.330848966847235	40.0	38.0	40.0	35.0	40.0
15	38.36661887515397	40.0	38.0	40.0	35.0	40.0
16	38.34010768348713	40.0	38.0	40.0	35.0	40.0
17	38.26612463943694	40.0	38.0	40.0	35.0	40.0
18	38.18870238886448	40.0	38.0	40.0	34.0	40.0
19	38.14096965909591	40.0	38.0	40.0	34.0	40.0
20	38.03556655357479	40.0	38.0	40.0	34.0	40.0
21	38.04500377948137	40.0	38.0	40.0	34.0	40.0
22	38.04688420311816	40.0	38.0	40.0	34.0	40.0
23	37.96103393863548	40.0	37.0	40.0	34.0	40.0
24	37.90890626211048	40.0	37.0	40.0	34.0	40.0
25	37.85776808324527	40.0	37.0	40.0	34.0	40.0
26	37.74051153032133	40.0	37.0	40.0	34.0	40.0
27	37.60431085025773	40.0	36.0	40.0	34.0	40.0
28	37.44228303900009	40.0	36.0	40.0	33.0	40.0
29	37.44171429389323	40.0	36.0	40.0	33.0	40.0
30	37.536499203729846	40.0	36.0	40.0	33.0	40.0
31	37.5334305187112	40.0	36.0	40.0	34.0	40.0
32	37.43887407913119	40.0	35.0	40.0	33.0	40.0
33	37.276894338447626	40.0	35.0	40.0	33.0	40.0
34	36.92973243324334	39.0	35.0	40.0	33.0	40.0
35	36.89780303973465	39.0	35.0	40.0	33.0	40.0
36	36.80387394815237	39.0	35.0	40.0	33.0	40.0
37	36.69577781024492	39.0	35.0	40.0	33.0	40.0
38	36.60112738999198	39.0	35.0	40.0	33.0	40.0
39	36.41975063254664	38.0	35.0	40.0	32.0	40.0
40	36.20151076632327	38.0	35.0	40.0	32.0	40.0
41	36.064949016835236	37.0	35.0	40.0	31.0	40.0
42	35.92615225571169	37.0	35.0	40.0	31.0	40.0
43	35.76169120921529	37.0	35.0	40.0	31.0	40.0
44	35.451205261621396	36.0	35.0	40.0	30.0	40.0
45	35.36858544774094	36.0	35.0	40.0	30.0	40.0
46	35.19633399758945	36.0	35.0	40.0	30.0	40.0
47	35.08552403272148	35.0	35.0	39.0	30.0	40.0
48	34.9493238927767	35.0	34.0	39.0	30.0	40.0
49	34.868177793069144	35.0	34.0	39.0	30.0	40.0
50	34.404993506421604	35.0	34.0	39.0	29.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	4.0
14	6.0
15	47.0
16	132.0
17	262.0
18	469.0
19	852.0
20	1472.0
21	2518.0
22	3800.0
23	5831.0
24	8811.0
25	12148.0
26	16520.0
27	22551.0
28	28958.0
29	38034.0
30	49639.0
31	64334.0
32	80273.0
33	107949.0
34	224887.0
35	320957.0
36	403521.0
37	677391.0
38	1039444.0
39	592077.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	0.0	100.0
2	100.0	0.0	0.0	0.0
3	0.0	0.0	0.0	100.0
4	0.0	100.0	0.0	0.0
5	0.0	0.0	0.0	100.0
6	0.0	0.0	0.0	100.0
7	99.29290345997408	0.31453818896542673	0.10670047090258444	0.2858578801578983
8	99.15355280701095	0.6113334750245012	0.12425433222545963	0.11085938573908102
9	94.56564860572381	2.8627917282964734	1.3344445377649723	1.2371151282147532
10	50.75990665666726	33.834446563210506	6.691018823410586	8.714627956711638
11	37.771264545061975	21.29696569354361	22.385224077740382	18.546545683654035
12	30.43553290417293	16.353474273735994	26.195357192721687	27.015635629369395
13	26.20191963626239	16.449399374380384	28.93694626006883	28.4117347292884
14	26.033753644789243	18.37195227834267	28.172948203416304	27.421345873451784
15	28.66983050261566	18.675012942596982	26.501847611419084	26.153308943368277
16	32.02804620932467	18.658647342655964	23.947490729535776	25.365815718483596
17	31.517633933936445	19.653735232139013	22.789503006976446	26.039127826948093
18	31.71858513717262	19.89530336988227	22.294619147373847	26.091492345571254
19	30.90543626881605	21.073680577516637	21.523221463025223	26.497661690642087
20	30.623953351018624	22.307365951288304	21.92185615069747	25.146824546995607
21	30.91999247074379	20.191639554952904	24.678973633830235	24.20939434047307
22	29.392617494070173	19.093253943069858	25.13026990547111	26.383858657388863
23	28.752333650833172	21.451817756351865	24.13739650310879	25.658452089706174
24	28.849879107907366	20.99101539365614	22.978922673620517	27.180182824815972
25	27.91334009742123	21.652336864540093	22.179897912143733	28.254425125894944
26	27.275918883876887	20.621223050434402	23.05580858621471	29.047049479474
27	26.81517053306216	19.65889336677389	22.47150805762744	31.054428042536518
28	28.454107049927774	22.4238695785912	21.826714222327485	27.29530914915354
29	29.799299952010443	20.803054047798895	22.443475891391827	26.95417010879883
30	29.76173468877949	20.368285411742022	23.183060577835306	26.68691932164318
31	32.60986219138624	20.598430036655163	21.80519048775159	24.986517284207007
32	33.53735421180597	20.388242801769106	21.857960095482206	24.216442890942723
33	32.90965513684045	20.767325188521717	22.777188298109934	23.545831376527897
34	29.30533429438474	22.96401539446632	25.297652724669845	22.432997586479097
35	28.24445993385165	25.12238417084606	24.431437183237197	22.201718712065095
36	29.006162485561948	25.339565944320775	21.28257152725885	24.37170004285843
37	30.263667098851734	25.30281085930472	20.886070308885845	23.5474517329577
38	31.405154191767565	24.530846050205664	20.77302344196653	23.290976316060245
39	30.21281491289639	22.42449071522263	20.50345014392816	26.85924422795282
40	28.356318539389108	23.2070688589369	21.514066449196832	26.92254615247716
41	26.62467062879822	22.793202820824497	21.697760856455595	28.884365693921694
42	26.062325929834785	21.10330609424155	23.424979792805022	29.40938818311864
43	26.360525524799687	20.564942670439216	23.656771780088466	29.417760024672628
44	26.925219740586336	20.44933023917271	23.560954703206065	29.06449531703489
45	28.152234647055312	21.249921345198302	22.87160106608651	27.72624294165988
46	27.045936294606726	22.636352318419483	23.083300633640384	27.23441075333341
47	26.767721095609403	22.216977068445747	24.559742406537165	26.45555942940769
48	27.730644909960844	21.501238627460882	25.00004726039587	25.768069202182403
49	28.172138025201406	20.723548558976518	24.611701836052877	26.492611579769203
50	25.708899186554067	21.567727252963834	24.467571131621824	28.255802428860278
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	81.5
10	163.0
11	111.5
12	60.0
13	111.0
14	162.0
15	1089.5
16	2017.0
17	3494.0
18	4971.0
19	6094.0
20	7217.0
21	6296.5
22	5376.0
23	5347.5
24	5319.0
25	6060.5
26	6802.0
27	7051.5
28	7301.0
29	8610.5
30	9920.0
31	12316.5
32	14713.0
33	15904.0
34	17095.0
35	20735.0
36	24375.0
37	26495.0
38	28615.0
39	34149.5
40	39684.0
41	43963.0
42	48242.0
43	53995.5
44	59749.0
45	65012.5
46	70276.0
47	74938.0
48	79600.0
49	93714.5
50	107829.0
51	116695.5
52	125562.0
53	140976.5
54	156391.0
55	168389.0
56	180387.0
57	192524.0
58	204661.0
59	225941.0
60	247221.0
61	291413.5
62	335606.0
63	354559.0
64	373512.0
65	383481.0
66	393450.0
67	377066.0
68	360682.0
69	311501.0
70	262320.0
71	221177.5
72	180035.0
73	149492.5
74	118950.0
75	100800.0
76	82650.0
77	68596.0
78	54542.0
79	54268.0
80	53994.0
81	38585.5
82	23177.0
83	14745.0
84	6313.0
85	4507.0
86	2701.0
87	1764.5
88	828.0
89	514.0
90	200.0
91	137.0
92	74.0
93	54.0
94	34.0
95	43.0
96	52.0
97	56.5
98	61.0
99	30.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	3702889.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	7.499412443111458
#Duplication Level	Percentage of deduplicated	Percentage of total
1	62.71947482935948	4.703592099607143
2	10.138508093808628	1.520657075065893
3	3.999007866961378	0.899706280727723
4	1.9063119845611007	0.5718487926988006
5	1.0984389109029746	0.41188232182117823
6	0.7016707429151274	0.315727098023098
7	0.5391804268713367	0.28304754816527355
8	0.43796898491011904	0.26276080441054733
9	0.37299928541260124	0.25175479340654494
>10	11.13369568421768	24.3751998815133
>50	4.800437931512678	24.55710353612322
>100	2.0277251108748153	26.42423708802083
>500	0.07474808861520801	3.852824706065335
>1k	0.04396946389129883	6.285601946810229
>5k	0.002931297592753255	1.5242744097834706
>10k+	0.002931297592753255	3.7597816177574477
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGCACCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	31934	0.8624077038226099	No Hit
CGCACCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC	20424	0.5515693287052352	No Hit
CGCACCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	15864	0.42842224004014157	No Hit
CGCACCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG	15179	0.40992317079988083	No Hit
CGCACCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	14104	0.38089178476589497	No Hit
CGCACCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT	13767	0.3717907828184966	No Hit
CGCACCGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC	12894	0.3482145967648504	No Hit
CGCACCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGT	12659	0.3418682007481186	No Hit
CGCACCGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG	9749	0.26328091390263114	No Hit
CGCACCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC	9185	0.24804956346247484	No Hit
CGCACCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC	7630	0.20605532599005805	No Hit
CGCACCGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGC	6161	0.1663835994003601	No Hit
CGCACCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA	6009	0.16227869644485698	No Hit
CGCACCGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCT	5981	0.1615225301109485	No Hit
CGCACCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTT	5494	0.14837063708904047	No Hit
CGCACCGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCC	5262	0.14210525889379888	No Hit
CGCACCGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCA	4602	0.12428133816595636	No Hit
CGCACCGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCG	4598	0.12417331440396943	No Hit
CGCACCGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG	4568	0.1233631361890675	No Hit
CGCACCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG	4551	0.12290403520062308	No Hit
CGCACCGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAA	4520	0.12206685104522441	No Hit
CGCACCGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTC	4504	0.12163475599727672	No Hit
CGCACCGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGC	4420	0.11936625699555131	No Hit
CGCACCGGGGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAA	4404	0.11893416194760362	No Hit
CGCACCGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGA	4159	0.1123177065259045	No Hit
CGCACCGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATC	4153	0.11215567088292411	No Hit
CGCACCGGGGTCTCTCGGGTGGAGTCTTCTGACTGCTGGTGGAGCAGGTC	4100	0.11072435603659736	No Hit
CGCACCGGGGGGGCTGGCGAGATGGCTCAGCGGGTAAGAGCACCCGATTG	3823	0.10324371051900286	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.00429394453898024	0.0	0.0	0.0	0.0
8	0.0066704673026925735	0.0	0.0	0.0	0.0
9	0.007291603934117387	0.0	0.0	0.0	0.0
10	0.01571745736909748	0.0	0.0	5.401188099346213E-5	0.0
11	0.022414930612286783	0.0	0.0	5.401188099346213E-5	0.0
12	0.029193421676966282	0.0	0.0	5.401188099346213E-5	0.0
13	0.03867250679131889	0.0	0.0	5.401188099346213E-5	0.0
14	0.04933985328752766	0.0	0.0	5.401188099346213E-5	0.0
15	0.060871389879631824	0.0	0.0	5.401188099346213E-5	0.0
16	0.0754545977478666	0.0	0.0	5.401188099346213E-5	0.0
17	0.08774230067387924	0.0	0.0	5.401188099346213E-5	0.0
18	0.10086718775529054	0.0	0.0	5.401188099346213E-5	0.0
19	0.12336313618906751	0.0	0.0	5.401188099346213E-5	0.0
20	0.13600191634153766	0.0	0.0	5.401188099346213E-5	0.0
21	0.148316625208047	0.0	0.0	5.401188099346213E-5	0.0
22	0.16003720338362828	0.0	0.0	5.401188099346213E-5	0.0
23	0.1730000548220592	0.0	0.0	5.401188099346213E-5	0.0
24	0.18771829239277765	0.0	0.0	5.401188099346213E-5	0.0
25	0.20257155966597973	0.0	0.0	5.401188099346213E-5	0.0
26	0.220719551679783	0.0	0.0	5.401188099346213E-5	0.0
27	0.2364370090488805	0.0	0.0	5.401188099346213E-5	0.0
28	0.2616335515323306	0.0	0.0	5.401188099346213E-5	0.0
29	0.2749204742567222	0.0	0.0	5.401188099346213E-5	0.0
30	0.2934465494374798	0.0	0.0	5.401188099346213E-5	0.0
31	0.3080297573057145	0.0	0.0	5.401188099346213E-5	0.0
32	0.3236932027938186	0.0	0.0	5.401188099346213E-5	0.0
33	0.3358188700768508	0.0	0.0	5.401188099346213E-5	0.0
34	0.35437195119810505	0.0	0.0	5.401188099346213E-5	0.0
35	0.3905869174042214	0.0	0.0	5.401188099346213E-5	0.0
36	0.40749263615517506	0.0	0.0	5.401188099346213E-5	0.0
37	0.42539757470450773	0.0	0.0	5.401188099346213E-5	0.0
38	0.4395216815842981	0.0	0.0	5.401188099346213E-5	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCACCG	377945	0.0	42.784	1
GCACCGG	375360	0.0	42.725807	2
CACCGGG	355665	0.0	42.69917	3
ACCGGAT	3230	0.0	42.63777	4
ACCGGGG	184215	0.0	42.60272	4
ACCGGGA	122350	0.0	42.523743	4
ACCGGGT	20515	0.0	42.380695	4
CCGGGAT	34045	0.0	42.268173	5
ACCGGGC	30020	0.0	42.182545	4
CGGGATA	3230	0.0	42.02477	6
CGGGATC	10350	0.0	41.91691	6
CCGGGGG	75880	0.0	41.903797	5
CACCGGA	10965	0.0	41.75285	3
CCGGGAC	22315	0.0	41.752186	5
CCGGGGT	31620	0.0	41.543957	5
CCGGGGA	45430	0.0	41.506054	5
CGGGGGG	34405	0.0	41.499783	6
CGGGATT	11325	0.0	41.047237	6
CGGGAGT	13995	0.0	40.88746	6
CGGGGGA	11720	0.0	40.7901	6
>>END_MODULE