FastQCFastQC Report
Fri 17 Jun 2016
SRR1527833_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527833_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5523429
Sequences flagged as poor quality0
Sequence length52
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCACCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC479210.8675951116598042No Hit
CGCACCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC304410.551125034828908No Hit
CGCACCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT221830.4016164596304216No Hit
CGCACCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG212050.3839100674599058No Hit
CGCACCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT207090.37493013850635176No Hit
CGCACCGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC195960.3547796124472678No Hit
CGCACCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC187360.33920957434231525No Hit
CGCACCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC184950.3348463427338344No Hit
CGCACCGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC148940.26965133434321326No Hit
CGCACCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA136460.24705667439556114No Hit
CGCACCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT112220.20317089257416No Hit
CGCACCGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCAT91560.16576659173133212No Hit
CGCACCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC87090.15767379285585095No Hit
CGCACCGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTG83270.15075779918597668No Hit
CGCACCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCT82480.14932752824377754No Hit
CGCACCGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCGC77300.13994929598986425No Hit
CGCACCGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT71080.12868817540697997No Hit
CGCACCGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGT69140.12517586448563023No Hit
CGCACCGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAG68540.12408958275737772No Hit
CGCACCGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA67650.12247826486046984No Hit
CGCACCGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG66690.12074021409526582No Hit
CGCACCGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGA66030.11954530419418807No Hit
CGCACCGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG65200.11804261447010543No Hit
CGCACCGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG62730.11357075468879929No Hit
CGCACCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGAT62580.11329918425673617No Hit
CGCACCGGGGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAA61500.11134387714588165No Hit
CGCACCGGGGTCTCTCGGGTGGAGTCTTCTGACTGCTGGTGGAGCAGGTCTC60390.10933425594861453No Hit
CGCACCGGGGGGGCTGGCGAGATGGCTCAGCGGGTAAGAGCACCCGATTGCT57240.10363127687528888No Hit
CGCACCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG56060.10149492280972562No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCACCG5645900.044.715951
GCACCGG5610000.044.667152
CACCGGG5305900.044.62373
ACCGGGG2742350.044.55664
ACCGGGT311400.044.404624
ACCGGGA1833150.044.358894
ACCGGAT49050.044.3588184
CGGGATA45600.043.881586
CCGGGAT505150.043.87375
CCGGGGG1133850.043.8457455
CGGGATC157050.043.65686
ACCGGGC445300.043.6447374
CACCGGA169300.043.5546383
CCGGGGT479350.043.5481345
CGGGGGG520100.043.5058636
CCGGGAC335300.043.3316425
CCGGGGA673950.043.218635
CGGGGTA54200.042.817346
CGGGATT168250.042.7738536
CGGGAGT203700.042.6804126
Produced by FastQC (version 0.11.5)