##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527833_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5523429
Sequences flagged as poor quality	0
Sequence length	52
%GC	61
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.37412773840308	33.0	31.0	34.0	31.0	34.0
2	32.7027082270814	34.0	31.0	34.0	31.0	34.0
3	32.906935890730196	34.0	31.0	34.0	31.0	34.0
4	36.37881685453004	37.0	37.0	37.0	35.0	37.0
5	36.27400732407351	37.0	37.0	37.0	35.0	37.0
6	36.299942662429444	37.0	37.0	37.0	35.0	37.0
7	36.45037168034567	37.0	37.0	37.0	35.0	37.0
8	36.60049346157975	37.0	37.0	37.0	35.0	37.0
9	38.634244053829605	39.0	39.0	39.0	38.0	39.0
10	38.04082880399115	39.0	38.0	39.0	37.0	39.0
11	37.759425711817784	39.0	38.0	39.0	35.0	39.0
12	37.27052017867886	39.0	37.0	39.0	35.0	39.0
13	37.17917818804225	39.0	37.0	39.0	34.0	39.0
14	37.98307935161292	40.0	38.0	40.0	34.0	40.0
15	38.0342676623525	40.0	38.0	40.0	34.0	40.0
16	38.01328232878525	40.0	38.0	40.0	34.0	40.0
17	37.927870893244034	40.0	38.0	40.0	33.0	40.0
18	37.90880845938275	40.0	38.0	40.0	34.0	40.0
19	37.8624041333744	40.0	37.0	40.0	34.0	40.0
20	37.823173430852464	40.0	37.0	40.0	33.0	40.0
21	37.81629147401008	40.0	37.0	40.0	33.0	40.0
22	37.78974184333681	40.0	37.0	40.0	33.0	40.0
23	37.73696719918007	40.0	37.0	40.0	33.0	40.0
24	37.68755170022101	40.0	37.0	40.0	33.0	40.0
25	37.631290453810486	40.0	36.0	40.0	33.0	40.0
26	37.49748770193298	40.0	36.0	40.0	33.0	40.0
27	37.37808614902084	40.0	36.0	40.0	33.0	40.0
28	37.24805695882033	40.0	35.0	40.0	33.0	40.0
29	37.115106576005594	39.0	35.0	40.0	33.0	40.0
30	36.957909117687585	39.0	35.0	40.0	32.0	40.0
31	36.84845862959404	39.0	35.0	40.0	32.0	40.0
32	36.65895243697348	39.0	35.0	40.0	32.0	40.0
33	36.454665027829634	38.0	35.0	40.0	31.0	40.0
34	36.212306702955715	38.0	35.0	40.0	30.0	40.0
35	36.11633986786107	38.0	35.0	40.0	30.0	40.0
36	36.0510163161326	38.0	35.0	40.0	30.0	40.0
37	35.93966628339027	38.0	35.0	40.0	30.0	40.0
38	35.85188349483627	37.0	35.0	40.0	30.0	40.0
39	35.67574961133745	37.0	34.0	40.0	30.0	40.0
40	35.43348072365916	37.0	34.0	40.0	29.0	40.0
41	35.295383900109876	37.0	34.0	40.0	29.0	40.0
42	35.15755973327438	36.0	34.0	40.0	29.0	40.0
43	34.97533090404529	36.0	34.0	40.0	29.0	40.0
44	34.815917249954694	35.0	34.0	39.0	28.0	40.0
45	34.649951325526224	35.0	34.0	39.0	28.0	40.0
46	34.479146921233166	35.0	33.0	39.0	28.0	40.0
47	34.26664577384809	35.0	33.0	39.0	27.0	40.0
48	34.03862365208279	35.0	33.0	39.0	26.0	40.0
49	34.35044552939849	35.0	33.0	39.0	28.0	40.0
50	34.41969345491723	35.0	34.0	39.0	29.0	40.0
51	34.3896255387731	35.0	34.0	38.0	29.0	40.0
52	33.93952126477954	35.0	33.0	37.0	28.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	5.0
14	13.0
15	48.0
16	143.0
17	348.0
18	758.0
19	1554.0
20	2829.0
21	5227.0
22	8532.0
23	13190.0
24	18893.0
25	26360.0
26	35835.0
27	47351.0
28	60623.0
29	78131.0
30	96376.0
31	121980.0
32	162238.0
33	224894.0
34	426929.0
35	516459.0
36	717095.0
37	1027966.0
38	1382845.0
39	546807.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	0.0	100.0
2	100.0	0.0	0.0	0.0
3	0.0	0.0	0.0	100.0
4	0.0	100.0	0.0	0.0
5	0.0	0.0	0.0	100.0
6	0.0	0.0	0.0	100.0
7	99.33099529296022	0.30691079762227413	0.07632939610520928	0.285764513312292
8	99.17319476723607	0.6024880558797805	0.1132086607793818	0.11110851610476029
9	94.50824840873305	2.876709377453752	1.3120472807743162	1.3029949330388786
10	50.67947827336967	34.005180477562035	6.682062899695099	8.633278349373189
11	37.85941667757475	21.34080115812116	22.255251221659588	18.544530942644506
12	30.480250583469072	16.40843034281784	26.15263091097939	26.95868816273369
13	26.203812885075557	16.44896675597713	28.946945819345192	28.400274539602123
14	26.0352038561553	18.418920565467577	28.112319358137857	27.433556220239275
15	28.769628431903442	18.66047341243999	26.44905908992403	26.120839065732536
16	32.08403692706107	18.587764955428955	24.00396565249594	25.324232465014035
17	31.565952961466508	19.62892978256804	22.80757116638957	25.99754608957588
18	31.783299830594363	19.854767753871734	22.278859744553607	26.08307267098029
19	31.00173823181216	21.012635448016077	21.58164792197021	26.40397839820155
20	30.67547351473152	22.254744290186405	21.907061718363718	25.16272047671836
21	30.904226342006027	20.193090198135977	24.691636300566188	24.21104715929181
22	29.388320190229656	19.07425622742684	25.162521325068177	26.37490225727533
23	28.735899384241204	21.403805498359805	24.20481914404983	25.655475973349162
24	28.803683364084158	20.94047013186917	23.078236363679157	27.177610140367513
25	27.926257402783673	21.6161554715377	22.198040383971623	28.25954674170701
26	27.266703346779693	20.610964674299247	23.11078860613579	29.011543372785276
27	26.77899906018526	19.587723495676325	22.522277375159526	31.11100006897889
28	28.459259637446234	22.392195138201288	21.850502649712706	27.298042574639776
29	29.78298444679926	20.679599574829332	22.479242514025255	27.05817346434615
30	29.800383059146775	20.37022653862302	23.17640002252224	26.65299037970797
31	32.7081238846376	20.533675729334078	21.77839164765221	24.979808738376104
32	33.57892352739575	20.31627454611981	21.83478777404399	24.27001415244045
33	33.01849267909482	20.651356249894768	22.753275184672418	23.576875886337998
34	29.25715167154317	22.891939047283852	25.380664800796755	22.47024448037623
35	28.27529782676667	25.08729631538669	24.439799986566317	22.197605871280324
36	29.046050922352762	25.313297228949626	21.263258747419403	24.377393101278212
37	30.288630486605335	25.262622186326645	20.902504585466744	23.54624274160128
38	31.459479247402296	24.491706148481317	20.807310096680883	23.241504507435508
39	30.267592830468175	22.372949846915745	20.50974132192158	26.849716000694496
40	28.373624427868993	23.16667780105438	21.533217861585623	26.926479909491007
41	26.586781508370976	22.800003403682748	21.659099809194615	28.954115278751658
42	26.020430424651064	21.076255347900734	23.433848792118084	29.469465435330118
43	26.353937019920053	20.553482266179216	23.581764154115135	29.510816559785596
44	26.75792519465716	20.35147007411519	23.596139282319008	29.294465448908642
45	28.153507540334093	21.160261134885594	22.847347182339085	27.838884142441227
46	26.96922509549774	22.560840376512488	23.150383575130594	27.319550952859174
47	26.80456289019013	22.149248229677614	24.568307115018587	26.477881765113665
48	27.85588445148838	21.35052337958902	25.001715419895866	25.791876749026738
49	28.215081609630538	20.658543813996705	24.62707495651705	26.49929961985571
50	25.66952521703456	21.54049594916491	24.558838359287318	28.231140474513204
51	25.683067529246777	21.81887374672509	24.848404858648497	27.64965386537964
52	25.94008178615132	21.529995225791804	24.765105154786998	27.764817833269877
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	118.0
10	236.0
11	164.0
12	92.0
13	155.5
14	1532.5
15	2846.0
16	4895.0
17	6944.0
18	8473.5
19	10003.0
20	8683.0
21	7363.0
22	7169.0
23	6975.0
24	7794.0
25	8613.0
26	8867.0
27	9121.0
28	10966.0
29	12811.0
30	14647.5
31	16484.0
32	19790.0
33	23096.0
34	25419.0
35	27742.0
36	33288.5
37	38835.0
38	39601.0
39	51872.5
40	63378.0
41	70006.0
42	76634.0
43	82112.5
44	87591.0
45	94298.0
46	101005.0
47	116718.0
48	132431.0
49	139310.5
50	146190.0
51	170167.0
52	194144.0
53	206575.5
54	219007.0
55	231420.5
56	243834.0
57	282618.5
58	321403.0
59	336810.0
60	352217.0
61	398330.0
62	444443.0
63	503926.5
64	559762.5
65	556115.0
66	544713.5
67	533312.0
68	464337.0
69	395362.0
70	347896.0
71	300430.0
72	246677.5
73	192925.0
74	166749.0
75	140573.0
76	116844.5
77	93116.0
78	90061.5
79	87007.0
80	64920.0
81	42833.0
82	29308.5
83	15784.0
84	10648.5
85	5513.0
86	3781.5
87	2050.0
88	1325.5
89	381.0
90	161.0
91	107.5
92	54.0
93	61.5
94	69.0
95	57.5
96	46.0
97	45.0
98	44.0
99	22.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	5523429.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	7.62447825300777
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.54255869221898	5.2260124815433215
2	9.543414703052285	1.4552711572571349
3	4.213946750348269	0.9638743607208942
4	2.09029857939357	0.6374974424351724
5	1.1881088886818456	0.4529355191979981
6	0.7175932160194478	0.32827643220277125
7	0.4776967429003103	0.25495318994432414
8	0.3242053227014279	0.19775171459571225
9	0.28848453670360663	0.19795896687831036
>10	5.268017731451244	11.63683873359731
>50	4.5678256348677975	24.98747414143888
>100	2.6165677304891823	34.92045366551126
>500	0.10696756396860678	5.411328367789317
>1k	0.046724190913116484	6.449274672669375
>5k	0.004980751315611402	2.557242762961456
>10k+	0.0026089649748440675	4.322856391256824
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGCACCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	47921	0.8675951116598042	No Hit
CGCACCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	30441	0.551125034828908	No Hit
CGCACCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT	22183	0.4016164596304216	No Hit
CGCACCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	21205	0.3839100674599058	No Hit
CGCACCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	20709	0.37493013850635176	No Hit
CGCACCGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	19596	0.3547796124472678	No Hit
CGCACCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC	18736	0.33920957434231525	No Hit
CGCACCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	18495	0.3348463427338344	No Hit
CGCACCGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	14894	0.26965133434321326	No Hit
CGCACCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	13646	0.24705667439556114	No Hit
CGCACCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	11222	0.20317089257416	No Hit
CGCACCGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCAT	9156	0.16576659173133212	No Hit
CGCACCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	8709	0.15767379285585095	No Hit
CGCACCGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTG	8327	0.15075779918597668	No Hit
CGCACCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCT	8248	0.14932752824377754	No Hit
CGCACCGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCGC	7730	0.13994929598986425	No Hit
CGCACCGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	7108	0.12868817540697997	No Hit
CGCACCGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGT	6914	0.12517586448563023	No Hit
CGCACCGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAG	6854	0.12408958275737772	No Hit
CGCACCGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA	6765	0.12247826486046984	No Hit
CGCACCGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG	6669	0.12074021409526582	No Hit
CGCACCGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGA	6603	0.11954530419418807	No Hit
CGCACCGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG	6520	0.11804261447010543	No Hit
CGCACCGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG	6273	0.11357075468879929	No Hit
CGCACCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGAT	6258	0.11329918425673617	No Hit
CGCACCGGGGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAA	6150	0.11134387714588165	No Hit
CGCACCGGGGTCTCTCGGGTGGAGTCTTCTGACTGCTGGTGGAGCAGGTCTC	6039	0.10933425594861453	No Hit
CGCACCGGGGGGGCTGGCGAGATGGCTCAGCGGGTAAGAGCACCCGATTGCT	5724	0.10363127687528888	No Hit
CGCACCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	5606	0.10149492280972562	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0031502170119322615	0.0	0.0	0.0	0.0
8	0.004870163081665393	0.0	0.0	0.0	0.0
9	0.00535898985937902	0.0	0.0	0.0	0.0
10	0.01236550700660767	0.0	0.0	1.8104695470875068E-5	0.0
11	0.01817711425275857	0.0	0.0	1.8104695470875068E-5	0.0
12	0.02447754827662309	0.0	0.0	1.8104695470875068E-5	0.0
13	0.03222635793815762	0.0	0.0	1.8104695470875068E-5	0.0
14	0.04256413905202728	0.0	0.0	1.8104695470875068E-5	0.0
15	0.053245909379843574	0.0	0.0	1.8104695470875068E-5	0.0
16	0.067096001415063	0.0	0.0	1.8104695470875068E-5	0.0
17	0.07978739294014642	0.0	0.0	1.8104695470875068E-5	0.0
18	0.09171838725545309	0.0	0.0	1.8104695470875068E-5	0.0
19	0.11210427435565841	0.0	0.0	1.8104695470875068E-5	0.0
20	0.12425252501661559	0.0	0.0	1.8104695470875068E-5	0.0
21	0.1354412268176164	0.0	0.0	1.8104695470875068E-5	0.0
22	0.1462497300137288	0.0	0.0	1.8104695470875068E-5	0.0
23	0.15904974971163746	0.0	0.0	1.8104695470875068E-5	0.0
24	0.1734067732200414	0.0	0.0	1.8104695470875068E-5	0.0
25	0.18598953657229955	0.0	0.0	1.8104695470875068E-5	0.0
26	0.20293553153303862	0.0	0.0	1.8104695470875068E-5	0.0
27	0.21613385453130654	0.0	0.0	1.8104695470875068E-5	0.0
28	0.23903629430196352	0.0	0.0	1.8104695470875068E-5	0.0
29	0.25134748722215855	0.0	0.0	1.8104695470875068E-5	0.0
30	0.267659817841417	0.0	0.0	1.8104695470875068E-5	0.0
31	0.28067709388497614	0.0	0.0	1.8104695470875068E-5	0.0
32	0.2949798033069675	0.0	0.0	1.8104695470875068E-5	0.0
33	0.30756256665922566	0.0	0.0	1.8104695470875068E-5	0.0
34	0.3248706555293822	0.0	0.0	1.8104695470875068E-5	0.0
35	0.3581289811093797	0.0	0.0	1.8104695470875068E-5	0.0
36	0.3737171239098031	0.0	0.0	1.8104695470875068E-5	0.0
37	0.3899751404426489	0.0	0.0	1.8104695470875068E-5	0.0
38	0.40230443805831484	0.0	0.0	1.8104695470875068E-5	0.0
39	0.4141268042007963	0.0	0.0	1.8104695470875068E-5	0.0
40	0.42683630042135057	0.0	0.0	1.8104695470875068E-5	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCACCG	564590	0.0	44.71595	1
GCACCGG	561000	0.0	44.66715	2
CACCGGG	530590	0.0	44.6237	3
ACCGGGG	274235	0.0	44.5566	4
ACCGGGT	31140	0.0	44.40462	4
ACCGGGA	183315	0.0	44.35889	4
ACCGGAT	4905	0.0	44.358818	4
CGGGATA	4560	0.0	43.88158	6
CCGGGAT	50515	0.0	43.8737	5
CCGGGGG	113385	0.0	43.845745	5
CGGGATC	15705	0.0	43.6568	6
ACCGGGC	44530	0.0	43.644737	4
CACCGGA	16930	0.0	43.554638	3
CCGGGGT	47935	0.0	43.548134	5
CGGGGGG	52010	0.0	43.505863	6
CCGGGAC	33530	0.0	43.331642	5
CCGGGGA	67395	0.0	43.21863	5
CGGGGTA	5420	0.0	42.81734	6
CGGGATT	16825	0.0	42.773853	6
CGGGAGT	20370	0.0	42.680412	6
>>END_MODULE