FastQCFastQC Report
Fri 17 Jun 2016
SRR1527832_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527832_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5552206
Sequences flagged as poor quality0
Sequence length52
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCACCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC453000.8158919175549322No Hit
CGCACCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC288150.518982905173187No Hit
CGCACCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT213470.3844778093608198No Hit
CGCACCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG205640.3703753066799034No Hit
CGCACCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT197910.3564529125900588No Hit
CGCACCGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC183720.3308955035169805No Hit
CGCACCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC176540.3179637066780303No Hit
CGCACCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC175520.3161265990490987No Hit
CGCACCGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC141200.25431333059328126No Hit
CGCACCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA129400.23306051684681728No Hit
CGCACCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT105750.19046483505835338No Hit
CGCACCGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCAT87780.15809932124276369No Hit
CGCACCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC82910.1493280328575705No Hit
CGCACCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCT79940.14397880770274013No Hit
CGCACCGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTG77780.14008846213559079No Hit
CGCACCGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCGC74960.13500939986736804No Hit
CGCACCGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT66080.11901575698019849No Hit
CGCACCGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGT66040.11894371354376981No Hit
CGCACCGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA65410.1178090294200179No Hit
CGCACCGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG64500.11617004124126519No Hit
CGCACCGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAG64150.11553966117251414No Hit
CGCACCGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGA63510.11438696618965506No Hit
CGCACCGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG61710.11114501155036394No Hit
CGCACCGGGGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAA59700.1075248288698222No Hit
CGCACCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGAT59520.10720063340589309No Hit
CGCACCGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG58530.10541755835428296No Hit
CGCACCGGGGTCTCTCGGGTGGAGTCTTCTGACTGCTGGTGGAGCAGGTCTC57120.10287802722017157No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCACCG5669150.044.7423131
GCACCGG5629200.044.6982542
CACCGGG5320400.044.6568533
ACCGGGG2755350.044.5842824
ACCGGGA1828850.044.4418074
ACCGGGT313300.044.3188634
ACCGGAT49250.044.225384
CGGGATA45550.043.828766
ACCGGGC447350.043.778924
CCGGGAT494600.043.7678955
CCGGGGG1143750.043.68145
CCGGGGT480950.043.565865
CACCGGA166650.043.4467473
CGGGATC149800.043.4359176
CGGGGGG518350.043.315526
CCGGGGA677300.043.2799345
CCGGGAC342650.043.214365
CGGGAGT208300.043.0297666
CGGGATT166200.043.010836
CCGGGGC530100.042.6677975
Produced by FastQC (version 0.11.5)