##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527832_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5552206
Sequences flagged as poor quality	0
Sequence length	52
%GC	61
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.32334084866448	33.0	31.0	34.0	31.0	34.0
2	32.67479989034989	34.0	31.0	34.0	31.0	34.0
3	32.8898038005074	34.0	31.0	34.0	31.0	34.0
4	36.362728616337364	37.0	37.0	37.0	35.0	37.0
5	36.278376558794825	37.0	37.0	37.0	35.0	37.0
6	36.24919122237179	37.0	37.0	37.0	35.0	37.0
7	36.409263813338335	37.0	37.0	37.0	35.0	37.0
8	36.57097935487264	37.0	37.0	37.0	35.0	37.0
9	38.590071045634836	39.0	39.0	39.0	37.0	39.0
10	37.9475001467885	39.0	38.0	39.0	35.0	39.0
11	37.725538101432115	39.0	38.0	39.0	35.0	39.0
12	37.21595956634174	39.0	37.0	39.0	35.0	39.0
13	36.98770362627035	39.0	37.0	39.0	33.0	39.0
14	37.918662780163416	40.0	38.0	40.0	33.0	40.0
15	37.980306746543626	40.0	38.0	40.0	34.0	40.0
16	37.99237960551176	40.0	38.0	40.0	34.0	40.0
17	37.94215110174226	40.0	38.0	40.0	34.0	40.0
18	37.93578570391661	40.0	38.0	40.0	34.0	40.0
19	37.84482528205906	40.0	37.0	40.0	34.0	40.0
20	37.817718758994175	40.0	37.0	40.0	33.0	40.0
21	37.866196247041266	40.0	37.0	40.0	34.0	40.0
22	37.72694565007134	40.0	37.0	40.0	33.0	40.0
23	37.700916176381064	40.0	37.0	40.0	33.0	40.0
24	37.701178234381075	40.0	37.0	40.0	34.0	40.0
25	37.61808657675886	40.0	36.0	40.0	33.0	40.0
26	37.43798158785895	40.0	36.0	40.0	33.0	40.0
27	37.31557942914942	40.0	35.0	40.0	33.0	40.0
28	37.20656816407749	39.0	35.0	40.0	33.0	40.0
29	37.06465376104561	39.0	35.0	40.0	32.0	40.0
30	36.89889604240189	39.0	35.0	40.0	32.0	40.0
31	36.80989664288393	39.0	35.0	40.0	32.0	40.0
32	36.62568445767322	39.0	35.0	40.0	31.0	40.0
33	36.240241266264256	38.0	35.0	40.0	31.0	40.0
34	36.10872399186918	38.0	35.0	40.0	30.0	40.0
35	36.09465733079789	38.0	35.0	40.0	30.0	40.0
36	35.89095757614181	38.0	35.0	40.0	30.0	40.0
37	35.84762182815263	38.0	35.0	40.0	30.0	40.0
38	35.48295344228943	37.0	34.0	40.0	29.0	40.0
39	35.47405589778189	37.0	34.0	40.0	29.0	40.0
40	35.320428312638256	37.0	34.0	40.0	29.0	40.0
41	34.99704315726038	36.0	34.0	40.0	28.0	40.0
42	34.99334066495371	36.0	34.0	40.0	28.0	40.0
43	34.928603153413256	36.0	34.0	40.0	29.0	40.0
44	34.76519729275174	35.0	34.0	39.0	28.0	40.0
45	34.59619743936014	35.0	33.0	39.0	28.0	40.0
46	34.25585163806962	35.0	33.0	39.0	27.0	40.0
47	33.95519870840527	35.0	33.0	39.0	26.0	40.0
48	33.89128933616656	35.0	33.0	38.0	26.0	40.0
49	33.86282893682259	35.0	33.0	38.0	26.0	40.0
50	33.712012486568405	35.0	33.0	38.0	26.0	40.0
51	33.99486546428573	35.0	33.0	38.0	27.0	40.0
52	33.739987133042256	35.0	33.0	37.0	27.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	2.0
14	5.0
15	57.0
16	127.0
17	373.0
18	836.0
19	1584.0
20	2979.0
21	5423.0
22	8919.0
23	13942.0
24	20086.0
25	27600.0
26	37542.0
27	49537.0
28	63751.0
29	81394.0
30	100957.0
31	128501.0
32	171160.0
33	242635.0
34	444576.0
35	534921.0
36	747130.0
37	1050304.0
38	1342040.0
39	475823.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	0.0	100.0
2	100.0	0.0	0.0	0.0
3	0.0	0.0	0.0	100.0
4	0.0	100.0	0.0	0.0
5	0.0	0.0	0.0	100.0
6	0.0	0.0	0.0	100.0
7	99.31785672217494	0.3090123097017654	0.08160720261460039	0.2915237655087005
8	99.1711402638879	0.6043723882002937	0.11300013003840276	0.11148721787340023
9	94.4964938260576	2.870462659346573	1.3284629568859656	1.3045805577098546
10	50.732843846211765	33.93905053234697	6.712232939483874	8.615872681957406
11	37.80054270320662	21.317941012995554	22.282728702789488	18.59878758100834
12	30.49528421675997	16.408378939830403	26.15835939804827	26.937977445361355
13	26.441958385549814	16.44681411316511	28.885923901238534	28.22530360004654
14	26.05764267392096	18.399677533578547	28.071959145608073	27.470720646892428
15	28.72200707250415	18.690588929877602	26.460833765894133	26.12657023172411
16	32.06307546946205	18.58466706746832	24.004530811717	25.34772665135263
17	31.518841339820604	19.6402294871624	22.827557190781466	26.01337198223553
18	31.7423020687633	19.854450645383114	22.3430290590803	26.060218226773284
19	30.99232989554062	21.000373545217883	21.571641974379194	26.435654584862306
20	30.667071790924183	22.266771081620533	21.91021010387583	25.155947023579454
21	30.90351834928315	20.178646109312226	24.653227924180047	24.264607617224577
22	29.515601546484405	19.074544424324312	25.070413453679492	26.339440575511787
23	28.720710290648437	21.40181758385766	24.19431123412928	25.683160891364622
24	28.807360533813046	20.928474195662048	23.07037959326437	27.193785677260536
25	27.896389291031348	21.591814136579227	22.250381199833	28.26141537255642
26	27.376379766889052	20.590842630838985	23.084950378282073	28.947827223989886
27	26.78088673222859	19.600335434240012	22.52472260575346	31.094055227777933
28	28.461804190982825	22.38418387214019	21.838508873770174	27.31550306310681
29	29.829296679554034	20.734443210500476	22.470942180459446	26.965317929486044
30	29.810565386082576	20.354576181071092	23.180119757804377	26.654738675041955
31	32.72353367292208	20.520636302039225	21.76181863569183	24.994011389346866
32	33.57011609439564	20.3337556279432	21.845767249990363	24.250361027670802
33	32.972641865233385	20.718251448163127	22.772966276827624	23.536140409775864
34	29.25075186331343	22.888073677381566	25.35005005217746	22.511124407127543
35	28.26831353159447	25.08302105505451	24.448660586440777	22.20000482691024
36	29.24410585630288	25.22581114605618	21.268645291619222	24.261437706021717
37	30.3002626343475	25.254682553204976	20.928366130507406	23.516688681940114
38	31.49330194160663	24.460025438537404	20.701069088574883	23.345603531281082
39	30.226400101148986	22.420673872691324	20.44990765832536	26.903018367834335
40	28.356908947542653	23.16077609512327	21.53284658386234	26.949468373471735
41	26.803490360408098	22.772804179095658	21.668846580980606	28.754858879515638
42	26.00541118251016	21.100910160754122	23.413792643860837	29.479886012874886
43	26.362782648914685	20.52549923399816	23.60499592414258	29.50672219294457
44	26.76040838542374	20.377774167601128	23.58763345596327	29.27418399101186
45	28.157672824099105	21.1884789577332	22.82701686500825	27.82683135315945
46	27.109837062962	22.55919539008459	23.137199880551982	27.193767666401424
47	27.047591533887612	22.16904416010501	24.445994979292916	26.33736932671446
48	27.85247881652806	21.368605559664033	24.975910475944158	25.80300514786375
49	28.208787642245266	20.66967616115108	24.613441936412304	26.508094260191356
50	25.742524682981866	21.51087333575159	24.489995508091738	28.256606473174806
51	25.64532007638045	21.831880877618733	24.90617242948118	27.61662661651963
52	25.96677788972527	21.49261032461692	24.770982921022743	27.769628864635067
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	114.5
10	229.0
11	167.0
12	105.0
13	145.5
14	1474.5
15	2763.0
16	4681.0
17	6599.0
18	8224.5
19	9850.0
20	8663.0
21	7476.0
22	7259.0
23	7042.0
24	7836.5
25	8631.0
26	8973.5
27	9316.0
28	10860.5
29	12405.0
30	14386.0
31	16367.0
32	19459.5
33	22552.0
34	24908.0
35	27264.0
36	33117.0
37	38970.0
38	39961.5
39	52234.5
40	63516.0
41	70566.0
42	77616.0
43	82905.0
44	88194.0
45	95273.5
46	102353.0
47	117552.0
48	132751.0
49	140423.0
50	148095.0
51	171174.5
52	194254.0
53	207280.5
54	220307.0
55	233043.0
56	245779.0
57	284058.0
58	322337.0
59	338737.0
60	355137.0
61	401392.5
62	447648.0
63	505945.5
64	561198.0
65	558153.0
66	546448.5
67	534744.0
68	467330.0
69	399916.0
70	351410.5
71	302905.0
72	248631.5
73	194358.0
74	167909.0
75	141460.0
76	117729.0
77	93998.0
78	89806.5
79	85615.0
80	64522.5
81	43430.0
82	29693.0
83	15956.0
84	10791.0
85	5626.0
86	3861.5
87	2097.0
88	1354.5
89	385.0
90	158.0
91	108.5
92	59.0
93	64.5
94	70.0
95	63.5
96	57.0
97	55.5
98	54.0
99	27.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	5552206.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	8.103584120552467
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.29027993346517	5.533960280569112
2	10.10939998248903	1.6384474633282302
3	4.282039241404196	1.0409959560067672
4	2.084944732637224	0.6758210011051408
5	1.1091028152295166	0.44938539807769745
6	0.676226555598904	0.32879152667083017
7	0.4648226758256325	0.2636710758285312
8	0.3536176376988551	0.22924562188829747
9	0.2674432044717197	0.1950523654416025
>10	5.487248660215352	12.967940157180625
>50	4.365639830121923	25.16091180057321
>100	2.364941257333704	33.486671428867574
>500	0.09363972596467447	5.026007254247811
>1k	0.0436830483408407	6.377606022162576
>5k	0.004414776162106241	2.350695787004861
>10k+	0.0025559230412194023	4.274796861047184
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGCACCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	45300	0.8158919175549322	No Hit
CGCACCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	28815	0.518982905173187	No Hit
CGCACCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT	21347	0.3844778093608198	No Hit
CGCACCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	20564	0.3703753066799034	No Hit
CGCACCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	19791	0.3564529125900588	No Hit
CGCACCGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	18372	0.3308955035169805	No Hit
CGCACCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	17654	0.3179637066780303	No Hit
CGCACCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC	17552	0.3161265990490987	No Hit
CGCACCGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	14120	0.25431333059328126	No Hit
CGCACCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	12940	0.23306051684681728	No Hit
CGCACCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	10575	0.19046483505835338	No Hit
CGCACCGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCAT	8778	0.15809932124276369	No Hit
CGCACCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	8291	0.1493280328575705	No Hit
CGCACCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCT	7994	0.14397880770274013	No Hit
CGCACCGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTG	7778	0.14008846213559079	No Hit
CGCACCGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCGC	7496	0.13500939986736804	No Hit
CGCACCGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	6608	0.11901575698019849	No Hit
CGCACCGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGT	6604	0.11894371354376981	No Hit
CGCACCGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA	6541	0.1178090294200179	No Hit
CGCACCGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG	6450	0.11617004124126519	No Hit
CGCACCGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAG	6415	0.11553966117251414	No Hit
CGCACCGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGA	6351	0.11438696618965506	No Hit
CGCACCGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG	6171	0.11114501155036394	No Hit
CGCACCGGGGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAAAAA	5970	0.1075248288698222	No Hit
CGCACCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGAT	5952	0.10720063340589309	No Hit
CGCACCGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG	5853	0.10541755835428296	No Hit
CGCACCGGGGTCTCTCGGGTGGAGTCTTCTGACTGCTGGTGGAGCAGGTCTC	5712	0.10287802722017157	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0034040523712556775	0.0	0.0	0.0	0.0
8	0.0051150839864371024	0.0	0.0	0.0	0.0
9	0.005619388041437944	0.0	0.0	0.0	0.0
10	0.012949807698057312	0.0	0.0	5.403257732151869E-5	0.0
11	0.019199575808246307	0.0	0.0	5.403257732151869E-5	0.0
12	0.025647463368614204	0.0	0.0	5.403257732151869E-5	0.0
13	0.03225744866094666	0.0	0.0	5.403257732151869E-5	0.0
14	0.04268573608399977	0.0	0.0	5.403257732151869E-5	0.0
15	0.05417666419437607	0.0	0.0	5.403257732151869E-5	0.0
16	0.06790093883404182	0.0	0.0	5.403257732151869E-5	0.0
17	0.08041848591352699	0.0	0.0	5.403257732151869E-5	0.0
18	0.09109892536408051	0.0	0.0	5.403257732151869E-5	0.0
19	0.11019043601768379	0.0	0.0	5.403257732151869E-5	0.0
20	0.12195152701466769	0.0	0.0	5.403257732151869E-5	0.0
21	0.13214567326932755	0.0	0.0	5.403257732151869E-5	0.0
22	0.14237584124220176	0.0	0.0	5.403257732151869E-5	0.0
23	0.1546412362941865	0.0	0.0	5.403257732151869E-5	0.0
24	0.16796927203349443	0.0	0.0	5.403257732151869E-5	0.0
25	0.18057687340851547	0.0	0.0	5.403257732151869E-5	0.0
26	0.1973089615190791	0.0	0.0	5.403257732151869E-5	0.0
27	0.21065500811749419	0.0	0.0	5.403257732151869E-5	0.0
28	0.23340272316985355	0.0	0.0	5.403257732151869E-5	0.0
29	0.24665871547273283	0.0	0.0	5.403257732151869E-5	0.0
30	0.26184186970007955	0.0	0.0	5.403257732151869E-5	0.0
31	0.2744314602159934	0.0	0.0	5.403257732151869E-5	0.0
32	0.28916434296566085	0.0	0.0	5.403257732151869E-5	0.0
33	0.30099747739907345	0.0	0.0	5.403257732151869E-5	0.0
34	0.3176395112141012	0.0	0.0	5.403257732151869E-5	0.0
35	0.3510676657170141	0.0	0.0	5.403257732151869E-5	0.0
36	0.3650260815250731	0.0	0.0	5.403257732151869E-5	0.0
37	0.38013719231599113	0.0	0.0	5.403257732151869E-5	0.0
38	0.39303296743672694	0.0	0.0	5.403257732151869E-5	0.0
39	0.404181689224067	0.0	0.0	5.403257732151869E-5	0.0
40	0.4162309539667656	0.0	0.0	5.403257732151869E-5	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCACCG	566915	0.0	44.742313	1
GCACCGG	562920	0.0	44.698254	2
CACCGGG	532040	0.0	44.656853	3
ACCGGGG	275535	0.0	44.584282	4
ACCGGGA	182885	0.0	44.441807	4
ACCGGGT	31330	0.0	44.318863	4
ACCGGAT	4925	0.0	44.22538	4
CGGGATA	4555	0.0	43.82876	6
ACCGGGC	44735	0.0	43.77892	4
CCGGGAT	49460	0.0	43.767895	5
CCGGGGG	114375	0.0	43.6814	5
CCGGGGT	48095	0.0	43.56586	5
CACCGGA	16665	0.0	43.446747	3
CGGGATC	14980	0.0	43.435917	6
CGGGGGG	51835	0.0	43.31552	6
CCGGGGA	67730	0.0	43.279934	5
CCGGGAC	34265	0.0	43.21436	5
CGGGAGT	20830	0.0	43.029766	6
CGGGATT	16620	0.0	43.01083	6
CCGGGGC	53010	0.0	42.667797	5
>>END_MODULE